PEC Original Annotations
|
Essentiality
|
Class
|
non-essential
|
References (PMID)
|
|
Deletion
|
DE(araA-leu)7697
,
OCR29
(D)
|
Related gene (W3110 PEC)
|
Gene Search
|
Search MG1655 PEC by gene name:
araB
|
Related strains
|
Strains Search
|
Search strains by gene name:
araB
Search strains by all related name:
araB b0063 ECK0064 JW0062
|
General information (Go to Linear View:)
|
Gene Name
|
araB
|
Alternative name
|
b0063,ECK0064,JW0062
|
Location, Length
|
68,348 - 70,048
(
-
)
;
1.47
min
;
1701
(bp) ,
566
(aa)
|
Product
|
L-ribulokinase
|
Operon Name
|
araBAD
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process
|
Function
|
enzyme; Degradation of small molecules: Carbon compounds
|
Gene Ontology
|
GO:0005515
;
protein binding ( araB )
GO:0005975
;
carbohydrate metabolic process ( araB )
GO:0008741
;
ribulokinase activity ( araB )
GO:0016301
;
kinase activity ( araB )
GO:0016740
;
transferase activity ( araB )
GO:0019568
;
arabinose catabolic process ( araB )
GO:0019572
;
L-arabinose catabolic process ( araB )
|
PID
|
1786249
|
EC number
(KEGG Pathway)
|
2.7.1.16
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
EcoGene
|
araB
(
EG10053
)
|
Number of removed
|
1 aa cleaved
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
ARAB_ECOLI
(
P08204
;
P78041
)
|
- Protein name
|
Ribulokinase
|
- Synonyms
|
EC 2.7.1.16
|
- Gene name
|
Name=araB; OrderedLocusNames=b0063;
|
Homology Analysis
|
BLAST
|
Bacteria
|
GTOP
|
araB
(
homologous genes of other bacterias
)
|
PDB
(database updated :
2007.02.20
)
|
PSI-BLAST
|
Chain O, Enterococcus Casseliflavus Glycerol Kinase
|
SWISS-PROT
(database updated :
2007.02.20
)
|
BLAST
|
Ribulokinase
|
PSI-BLAST
|
Glycerol kinase 2
|
nr
(database updated :
2007.02.20
)
|
BLAST
|
ribulokinase [Escherichia coli K12]
|
Pfam 28.0
(database updated :
2015-05
)
|
Pfam
|
|
PROSITE
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
|
MMBR References
|
J Mol Biol. 1984;180(1):201-4.
Deletion analysis of the Escherichia coli ara PC and PBAD promoters.
Dunn TM, Schleif R.
(
6392566
)
|
J Bacteriol. 1970;102(2):593-5.
Selection of araB and araC mutants of Escherichia coli B-r by resistance to ribitol.
Katz L.
(
4911550
)
|
Gene. 1986;47(2-3):231-44.
The organization of the araBAD operon of Escherichia coli.
Lee N, Gielow W, Martin R, Hamilton E, Fowler A.
(
3549454
)
|
Proc Natl Acad Sci U S A. 1981;78:752-756
Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and Pbad, in the L-arabinose regulatory region of Escherichia coli.
Lee, N., W. Gielow, and R. G. Wallace.
|
Gene. 1982;17(2):167-77.
The use of synthetic oligodeoxyribonucleotides to produce specific deletions in the araBAD promoter of Escherichia coli B/r.
Miyada CG, Soberon X, Itakura K, Wilcox G.
(
7044899
)
|
Proc Natl Acad Sci U S A. 1980;77:3346-3350
The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.
Ogden, S., D. Haggerty, C. M. Stoner, D. Kolodrubetz, and R. Schleif.
|
J Biol Chem. 1978;253:6931-6933
Nucleotide sequence of the L-arabinose regulatory region of Escherichia coli K12.
Smith, B. R., and R. Schleif.
|
Sequences |
Amino acid
FASTA format
|
0001 MAIAIGLDFG SDSVRALAVD CATGEEIATS VEWYPRWQKG QFCDAPNNQF RHHPRDYIES MEAALKTVLA
0071 ELSVEQRAAV VGIGVDSTGS TPAPIDADGN VLALRPEFAE NPNAMFVLWK DHTAVEEAEE ITRLCHAPGN
0141 VDYSRYIGGI YSSEWFWAKI LHVTRQDSAV AQSAASWIEL CDWVPALLSG TTRPQDIRRG RCSAGHKSLW
0211 HESWGGLPPA SFFDELDPIL NRHLPSPLFT DTWTADIPVG TLCPEWAQRL GLPESVVISG GAFDCHMGAV
0281 GAGAQPNALV KVIGTSTCDI LIADKQSVGE RAVKGICGQV DGSVVPGFIG LEAGQSAFGD IYAWFGRVLG
0351 WPLEQLAAQH PELKTQINAS QKQLLPALTE AWAKNPSLDH LPVVLDWFNG RRTPNANQRL KGVITDLNLA
0421 TDAPLLFGGL IAATAFGARA IMECFTDQGI AVNNVMALGG IARKNQVIMQ ACCDVLNRPL QIVASDQCCA
0491 LGAAIFAAVA AKVHADIPSA QQKMASAVEK TLQPCSEQAQ RFEQLYRRYQ QWAMSAEQHY LPTSAPAQAA
0561 QAVATL
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 atagcatttt tatccataag attagcggat
-070 cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
0001 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggac tgcgctaccg
0071 gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg caattttgtg atgccccgaa
0141 taaccagttc cgtcatcatc cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca
0211 gagcttagcg tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag taccggctcg acgcccgcac
0281 cgattgatgc cgacggaaac gtgctggcgc tgcgcccgga gtttgccgaa aacccgaacg cgatgttcgt
0351 attgtggaaa gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
0421 gttgactact cccgctacat tggtggtatt tattccagcg aatggttctg ggcaaaaatc ctgcatgtga
0491 ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg tgcgactggg tgccagctct
0561 gctttccggt accacccgcc cgcaggatat tcgtcgcgga cgttgcagcg ccgggcataa atctctgtgg
0631 cacgaaagct ggggcggcct gccgccagcc agtttctttg atgagctgga cccgatcctc aatcgccatt
0701 tgccttcccc gctgttcact gacacttgga ctgccgatat tccggtgggc accttatgcc cggaatgggc
0771 gcagcgtctc ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
0841 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt ctgattgccg
0911 acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt gatggcagcg tggtgcctgg
0981 atttatcggt ctggaagcag gccaatcggc gtttggtgat atctacgcct ggtttggtcg cgtactcggc
1051 tggccgctgg aacagcttgc cgcccagcat ccggaactga aaacgcaaat caacgccagc cagaaacaac
1121 tgcttccggc gctgaccgaa gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg
1191 gtttaacggc cgccgcacac cgaacgctaa ccaacgcctg aaaggggtga ttaccgatct taacctcgct
1261 accgacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca atcatggagt
1331 gctttaccga tcaggggatc gccgttaata acgtgatggc actgggcggc atcgcgcgga aaaaccaggt
1401 cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg caaattgttg cctctgacca gtgctgtgcg
1471 ctcggtgcgg cgatttttgc tgccgtcgcc gcgaaagtgc acgcagacat cccatcagct cagcaaaaaa
1541 tggccagtgc ggtagagaaa accctgcaac cgtgcagcga gcaggcacaa cgctttgaac agctttatcg
1611 ccgctatcag caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
1681 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg gtttgtcatt
1751 ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca t
|