MG1655
W3110
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Gene Report : ispE
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Tetrahedron lett. 2000;41:2925-2928
     Deletion OCR66,67-1 (D)  ,  OCR67 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  ispE
Related strains
     Strains Search Search strains by gene name:  ispE   Search strains by all related name:  ispE b1208 ECK1196 ipk JW1199 ychB

General information  (Go to Linear View:)
 Gene Name ispE  
 Alternative name b1208,ECK1196,ipk,JW1199,ychB  
 Location, Length 1,261,249 - 1,262,100 (  -  ) ;   27.18 min ; 852 (bp) ,   283 (aa) Go to Linear View
 Product 4-diphosphocytidyl-2-C-methylerythritol kinase  
 Operon Name lolB-ispE-prs  
 Note GO_component: GO:0005737 - cytoplasm  
 Function  
 Gene Ontology GO:0000166 ; nucleotide binding ( ispE )
GO:0005515 ; protein binding ( ispE )
GO:0005524 ; ATP binding ( ispE )
GO:0008299 ; isoprenoid biosynthetic process ( ispE )
GO:0016114 ; terpenoid biosynthetic process ( ispE )
GO:0016301 ; kinase activity ( ispE )
GO:0016310 ; phosphorylation ( ispE )
GO:0016740 ; transferase activity ( ispE )
GO:0050515 ; 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity ( ispE )
 PID 1787459  
 EC number
  (KEGG Pathway)
 
2.7.1.148  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) ISPE_ECOLI ( P62615 ; P24209 )
    -  Protein name 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase  
    -  Synonyms EC 2.7.1.148, CMK, 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase  
    -  Gene name Name=ispE; Synonyms=ipk; OrderedLocusNames=b1208;  

 Linear View (Whole Mode)
View Location
   1250.0  –  1275.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP ispE (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Ternary Complex Of 4-Diphosphocytidyl-2-C-Methyl-D-Erythritol Kinase  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase)  
         PSI-BLAST 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase)
    nr     (database updated : 2007.02.20 )
         BLAST 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase [Escherichia coli O157:H7 str. Sakai]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG1947I 
    EcoCyc ispE 

Sequences
Amino acid
FASTA format
0001 MRTQWPSPAK LNLFLYITGQ RADGYHTLQT LFQFLDYGDT ISIELRDDGD IRLLTPVEGV EHEDNLIVRA 
0071 ARLLMKTAAD SGRLPTGSGA NISIDKRLPM GGGLGGGSSN AATVLVALNH LWQCGLSMDE LAEMGLTLGA 
0141 DVPVFVRGHA AFAEGVGEIL TPVDPPEKWY LVAHPGVSIP TPVIFKDPEL PRNTPKRSIE TLLKCEFSND 
0211 CEVIARKRFR EVDAVLSWLL EYAPSRLTGT GACVFAEFDT ESEARQVLEQ APEWLNGFVA KGANLSPLHR 
0281 AML

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gttatgacac caaaacgcaa cctgcgatgc 
-070 cagccaatat ggaactcacc gacggtggtc aacgcatcaa gttaaaaatg gataactgga tagtgaaata 
0001 atgcggacac agtggccctc tccggcaaaa cttaatctgt ttttatacat taccggtcag cgtgcggatg 
0071 gttaccacac gctgcaaacg ctgtttcagt ttcttgatta cggcgacacc atcagcattg agcttcgtga 
0141 cgatggggat attcgtctgt taacgcccgt tgaaggcgtg gaacatgaag ataacctgat cgttcgcgca 
0211 gcgcgattgt tgatgaaaac tgcggcagac agcgggcgtc ttccgacggg aagcggtgcg aatatcagca 
0281 ttgacaagcg tttgccgatg ggcggcggtc tcggcggtgg ttcatccaat gccgcgacgg tcctggtggc 
0351 attaaatcat ctctggcaat gcgggctaag catggatgag ctggcggaaa tggggctgac gctgggcgca 
0421 gatgttcctg tctttgttcg ggggcatgcc gcgtttgccg aaggcgttgg tgaaatacta acgccggtgg 
0491 atccgccaga gaagtggtat ctggtggcgc accctggtgt aagtattccg actccggtga tttttaaaga 
0561 tcctgaactc ccgcgcaata cgccaaaaag gtcaatagaa acgttgctaa aatgtgaatt cagcaatgat 
0631 tgcgaggtta tcgcaagaaa acgttttcgc gaggttgatg cggtgctttc ctggctgtta gaatacgccc 
0701 cgtcgcgcct gactgggaca ggggcctgtg tctttgctga atttgataca gagtctgaag cccgccaggt 
0771 gctagagcaa gccccggaat ggctcaatgg ctttgtggcg aaaggcgcta atctttcccc attgcacaga 
0841 gccatgcttt aagccgggca agctgagttt cggtgacaac gtcaccttgt tccagacgtt gcatcgcgct 
0911 ctttaataca ccgcctggaa aggatcatgc ctggcccgca ca