MG1655
W3110
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Gene Report : prs
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Mol Biol. 1992;226(4):989-96.
dnaR function of the prs gene of Escherichia coli in initiation of chromosome replication.
Sakakibara Y. ( 1325564 )
     Deletion OCR66,67-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  prs
Related strains
     Strains Search Search strains by gene name:  prs   Search strains by all related name:  prs b1207 dnaR ECK1195 JW1198 prsA

General information  (Go to Linear View:)
 Gene Name prs  
 Alternative name b1207,dnaR,ECK1195,JW1198,prsA  
 Location, Length 1,260,151 - 1,261,098 (  -  ) ;   27.16 min ; 948 (bp) ,   315 (aa) Go to Linear View
 Product phosphoribosylpyrophosphate synthase  
 Operon Name lolB-ispE-prs  
 Note phosphoribosylpyrophosphate synthetase; GO_process: GO:0009152 - purine ribonucleotide biosynthetic process  
 Function enzyme; Purine ribonucleotide biosynthesis  
 Gene Ontology GO:0000287 ; magnesium ion binding ( prs )
GO:0004749 ; ribose phosphate diphosphokinase activity ( prs )
GO:0005515 ; protein binding ( prs )
GO:0005737 ; cytoplasm ( prs )
GO:0009116 ; nucleoside metabolic process ( prs )
GO:0009156 ; ribonucleoside monophosphate biosynthetic process ( prs )
GO:0009165 ; nucleotide biosynthetic process ( prs )
GO:0016301 ; kinase activity ( prs )
GO:0016740 ; transferase activity ( prs )
GO:0044249 ; cellular biosynthetic process ( prs )
GO:0046872 ; metal ion binding ( prs )
 PID 1787458  
 EC number
  (KEGG Pathway)
 
2.7.6.1  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) KPRS_ECOLI ( P08330 ; P76828; P78058 )
    -  Protein name Ribose-phosphate pyrophosphokinase  
    -  Synonyms EC 2.7.6.1, RPPK, Phosphoribosyl pyrophosphate synthetase, P-Rib-PP synthetase, PRPP synthetase  
    -  Gene name OrderedLocusNames=b1207, c1665, z1978, ECs1712;  

 Linear View (Whole Mode)
View Location
   1250.0  –  1275.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP prsA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Human Phosphoribosyl Pyrophosphate Synthetase 1 Mutant Y146m  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Ribose-phosphate pyrophosphokinase (RPPK) (Phosphoribosyl pyrophosphate synthetase) (P-Rib-PP synthetase) (PRPP synthetase)  
         PSI-BLAST Ribose-phosphate pyrophosphokinase (RPPK) (Phosphoribosyl pyrophosphate synthetase) (P-Rib-PP synthetase) (PRPP synthetase)
    nr     (database updated : 2007.02.20 )
         BLAST ribose-phosphate pyrophosphokinase [Escherichia coli O157:H7 str. Sakai]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    PUR_PYR_PR_TRANSFER    PRPP_SYNTHETASE    AA_TRNA_LIGASE_II_2     

Other Cross-Reference
    COG COG0462FE 
    EcoCyc prs 

MMBR References
Genes Genet Syst. 1996;71(1):47-50.
10Sa RNA complements the temperature-sensitive phenotype caused by a mutation in the phosphoribosyl pyrophosphate synthetase (prs) gene in Escherichia coli.
Ando H, Kitabatake M, Inokuchi H. ( 8925474 )
J Bacteriol. 1983;154(1):177-84.
Chromosomal location of the gene encoding phosphoribosylpyrophosphate synthetase in Escherichia coli.
Hove-Jensen B. ( 6300032 )
Mol Gen Genet. 1985;201(2):269-76.
Cloning and characterization of the prs gene encoding phosphoribosylpyrophosphate synthetase of Escherichia coli.
Hove-Jensen B. ( 3003529 )
J Biol Chem. 1986;261:6765-6771
Phosphoribosylpyrphosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene.
Hove-Jensen, B., K. W. Harlow, C. J. King, and R. L. Switzer.
Eur J Biochem. 1982;126:327-332
Phosphoribosyl-pyrophosphate synthetase of Escherichia coli. Identification of a mutant enzyme.
Hove-Jensen, B., and P. Nygaard.
J Gen Microbiol. 1993;139(2):259-66.
Characterization of the hemA-prs region of the Escherichia coli and Salmonella typhimurium chromosomes: identification of two open reading frames and implications for prs expression.
Post DA, Hove-Jensen B, Switzer RL. ( 7679718 )
J Bacteriol. 1995;177:733-737
Suppression of thermosensitive initiation of DNA replication in a dnaR mutant of Escherichia coli by a rifampin resistance mutation in the rpoB gene.
Sakakibara, Y.

Sequences
Amino acid
FASTA format
0001 MPDMKLFAGN ATPELAQRIA NRLYTSLGDA AVGRFSDGEV SVQINENVRG GDIFIIQSTC APTNDNLMEL 
0071 VVMVDALRRA SAGRITAVIP YFGYARQDRR VRSARVPITA KVVADFLSSV GVDRVLTVDL HAEQIQGFFD 
0141 VPVDNVFGSP ILLEDMLQLN LDNPIVVSPD IGGVVRARAI AKLLNDTDMA IIDKRRPRAN VSQVMHIIGD 
0211 VAGRDCVLVD DMIDTGGTLC KAAEALKERG AKRVFAYATH PIFSGNAANN LRNSVIDEVV VCDTIPLSDE 
0281 IKSLPNVRTL TLSGMLAEAI RRISNEESIS AMFEH

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             atcgcgctct ttaatacacc gcctggaaag 
-070 gatcatgcct ggcccgcaca gttttcggca gattctttcc accaatggac gcatgcctga ggttcttctc 
0001 gtgcctgata tgaagctttt tgctggtaac gccaccccgg aactagcaca acgtattgcc aaccgcctgt 
0071 acacttcact cggcgacgcc gctgtaggtc gctttagcga tggcgaagtc agcgtacaaa ttaatgaaaa 
0141 tgtacgcggt ggtgatattt tcatcatcca gtccacttgt gcccctacta acgacaacct gatggaatta 
0211 gtcgttatgg ttgatgccct gcgtcgtgct tccgcaggtc gtatcaccgc tgttatcccc tactttggct 
0281 atgcgcgcca ggaccgtcgc gtccgttccg ctcgtgtacc aatcactgcg aaagtggttg cagacttcct 
0351 ctccagcgtc ggtgttgacc gtgtgctgac agtggatctg cacgctgaac agattcaggg tttcttcgac 
0421 gttccggttg ataacgtatt tggtagcccg atcctgctgg aagacatgct gcagctgaat ctggataacc 
0491 caattgtggt ttctccggac atcggcggcg ttgtgcgtgc ccgcgctatc gctaagctgc tgaacgatac 
0561 cgatatggca atcatcgaca aacgtcgtcc gcgtgcgaac gtttcacagg tgatgcatat catcggtgac 
0631 gttgcaggtc gtgactgcgt actggtcgat gatatgatcg acactggcgg tacgctgtgt aaagctgctg 
0701 aagctctgaa agaacgtggt gctaaacgtg tatttgcgta cgcgactcac ccgatcttct ctggcaacgc 
0771 ggcgaacaac ctgcgtaact ctgtaattga tgaagtcgtt gtctgcgata ccattccgct gagcgatgaa 
0841 atcaaatcac tgccgaacgt gcgtactctg accctgtcag gtatgctggc cgaagcgatt cgtcgtatca 
0911 gcaacgaaga atcgatctct gccatgttcg aacactaatc gaacccggct caaagacccg ctgcggcggg 
0981 tttttttgtc tgtaatatcc atttgtatga cctatgcctc cttcacctgc catttagttg acagatga