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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Mol Biol. 1992;226(4):989-96.
dnaR function of the prs gene of Escherichia coli in initiation of chromosome replication.
Sakakibara Y.
(
1325564
)
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Deletion
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OCR66,67-1
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
prs
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Related strains
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Strains Search
|
Search strains by gene name:
prs
Search strains by all related name:
prs b1207 dnaR ECK1195 JW1198 prsA
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General information (Go to Linear View: )
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Gene Name
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prs
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Alternative name
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b1207,dnaR,ECK1195,JW1198,prsA
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Location, Length
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1,260,151 - 1,261,098
(
-
)
;
27.16
min
;
948
(bp) ,
315
(aa)
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Product
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phosphoribosylpyrophosphate synthase
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Operon Name
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lolB-ispE-prs
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Note
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phosphoribosylpyrophosphate synthetase; GO_process: GO:0009152 - purine ribonucleotide biosynthetic process
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Function
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enzyme; Purine ribonucleotide biosynthesis
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Gene Ontology
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GO:0000287
;
magnesium ion binding ( prs )
GO:0004749
;
ribose phosphate diphosphokinase activity ( prs )
GO:0005515
;
protein binding ( prs )
GO:0005737
;
cytoplasm ( prs )
GO:0009116
;
nucleoside metabolic process ( prs )
GO:0009156
;
ribonucleoside monophosphate biosynthetic process ( prs )
GO:0009165
;
nucleotide biosynthetic process ( prs )
GO:0016301
;
kinase activity ( prs )
GO:0016740
;
transferase activity ( prs )
GO:0044249
;
cellular biosynthetic process ( prs )
GO:0046872
;
metal ion binding ( prs )
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PID
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1787458
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EC number
(KEGG Pathway)
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2.7.6.1
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
|
KPRS_ECOLI
(
P08330
;
P76828; P78058
)
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- Protein name
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Ribose-phosphate pyrophosphokinase
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- Synonyms
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EC 2.7.6.1, RPPK, Phosphoribosyl pyrophosphate synthetase, P-Rib-PP synthetase, PRPP synthetase
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- Gene name
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OrderedLocusNames=b1207, c1665, z1978, ECs1712;
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MMBR References
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Genes Genet Syst. 1996;71(1):47-50.
10Sa RNA complements the temperature-sensitive phenotype caused by a mutation in the phosphoribosyl pyrophosphate synthetase (prs) gene in Escherichia coli.
Ando H, Kitabatake M, Inokuchi H.
(
8925474
)
|
J Bacteriol. 1983;154(1):177-84.
Chromosomal location of the gene encoding phosphoribosylpyrophosphate synthetase in Escherichia coli.
Hove-Jensen B.
(
6300032
)
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Mol Gen Genet. 1985;201(2):269-76.
Cloning and characterization of the prs gene encoding phosphoribosylpyrophosphate synthetase of Escherichia coli.
Hove-Jensen B.
(
3003529
)
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J Biol Chem. 1986;261:6765-6771
Phosphoribosylpyrphosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene.
Hove-Jensen, B., K. W. Harlow, C. J. King, and R. L. Switzer.
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Eur J Biochem. 1982;126:327-332
Phosphoribosyl-pyrophosphate synthetase of Escherichia coli. Identification of a mutant enzyme.
Hove-Jensen, B., and P. Nygaard.
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J Gen Microbiol. 1993;139(2):259-66.
Characterization of the hemA-prs region of the Escherichia coli and Salmonella typhimurium chromosomes: identification of two open reading frames and implications for prs expression.
Post DA, Hove-Jensen B, Switzer RL.
(
7679718
)
|
J Bacteriol. 1995;177:733-737
Suppression of thermosensitive initiation of DNA replication in a dnaR mutant of Escherichia coli by a rifampin resistance mutation in the rpoB gene.
Sakakibara, Y.
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| Sequences |
Amino acid
FASTA format
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0001 MPDMKLFAGN ATPELAQRIA NRLYTSLGDA AVGRFSDGEV SVQINENVRG GDIFIIQSTC APTNDNLMEL
0071 VVMVDALRRA SAGRITAVIP YFGYARQDRR VRSARVPITA KVVADFLSSV GVDRVLTVDL HAEQIQGFFD
0141 VPVDNVFGSP ILLEDMLQLN LDNPIVVSPD IGGVVRARAI AKLLNDTDMA IIDKRRPRAN VSQVMHIIGD
0211 VAGRDCVLVD DMIDTGGTLC KAAEALKERG AKRVFAYATH PIFSGNAANN LRNSVIDEVV VCDTIPLSDE
0281 IKSLPNVRTL TLSGMLAEAI RRISNEESIS AMFEH
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 atcgcgctct ttaatacacc gcctggaaag
-070 gatcatgcct ggcccgcaca gttttcggca gattctttcc accaatggac gcatgcctga ggttcttctc
0001 gtgcctgata tgaagctttt tgctggtaac gccaccccgg aactagcaca acgtattgcc aaccgcctgt
0071 acacttcact cggcgacgcc gctgtaggtc gctttagcga tggcgaagtc agcgtacaaa ttaatgaaaa
0141 tgtacgcggt ggtgatattt tcatcatcca gtccacttgt gcccctacta acgacaacct gatggaatta
0211 gtcgttatgg ttgatgccct gcgtcgtgct tccgcaggtc gtatcaccgc tgttatcccc tactttggct
0281 atgcgcgcca ggaccgtcgc gtccgttccg ctcgtgtacc aatcactgcg aaagtggttg cagacttcct
0351 ctccagcgtc ggtgttgacc gtgtgctgac agtggatctg cacgctgaac agattcaggg tttcttcgac
0421 gttccggttg ataacgtatt tggtagcccg atcctgctgg aagacatgct gcagctgaat ctggataacc
0491 caattgtggt ttctccggac atcggcggcg ttgtgcgtgc ccgcgctatc gctaagctgc tgaacgatac
0561 cgatatggca atcatcgaca aacgtcgtcc gcgtgcgaac gtttcacagg tgatgcatat catcggtgac
0631 gttgcaggtc gtgactgcgt actggtcgat gatatgatcg acactggcgg tacgctgtgt aaagctgctg
0701 aagctctgaa agaacgtggt gctaaacgtg tatttgcgta cgcgactcac ccgatcttct ctggcaacgc
0771 ggcgaacaac ctgcgtaact ctgtaattga tgaagtcgtt gtctgcgata ccattccgct gagcgatgaa
0841 atcaaatcac tgccgaacgt gcgtactctg accctgtcag gtatgctggc cgaagcgatt cgtcgtatca
0911 gcaacgaaga atcgatctct gccatgttcg aacactaatc gaacccggct caaagacccg ctgcggcggg
0981 tttttttgtc tgtaatatcc atttgtatga cctatgcctc cttcacctgc catttagttg acagatga
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