|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
DE(gpt-lac)5
,
OCR36,37-1
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
proB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
proB
Search strains by all related name:
proB b0242 ECK0243 JW0232 pro pro-2
|
General information (Go to Linear View: )
|
|
Gene Name
|
proB
|
|
Alternative name
|
b0242,ECK0243,JW0232,pro,pro-2
|
|
Location, Length
|
259,612 - 260,715
(
+
)
;
5.60
min
;
1104
(bp) ,
367
(aa)
|
|
Product
|
gamma-glutamate kinase
|
|
Operon Name
|
proBA
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006561 - proline biosynthetic process
|
|
Function
|
enzyme; Amino acid biosynthesis: Proline
|
|
Gene Ontology
|
GO:0003723
;
RNA binding ( proB )
GO:0004349
;
glutamate 5-kinase activity ( proB )
GO:0005737
;
cytoplasm ( proB )
GO:0006561
;
proline biosynthetic process ( proB )
GO:0008652
;
amino acid biosynthetic process ( proB )
GO:0016301
;
kinase activity ( proB )
GO:0016740
;
transferase activity ( proB )
|
|
PID
|
1786437
|
EC number
(KEGG Pathway)
|
2.7.2.11
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
proB
(
EG10768
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
PROB_ECOLI
(
P07005
;
P78293
)
|
|
- Protein name
|
Glutamate 5-kinase
|
|
- Synonyms
|
EC 2.7.2.11, Gamma-glutamyl kinase, GK
|
|
- Gene name
|
Name=proB; OrderedLocusNames=b0242, c0389, z0303, ECs0269;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
proB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Glutamate 5-Kinase From Campylobacter Jejuni
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Glutamate 5-kinase (Gamma-glutamyl kinase) (GK)
|
|
PSI-BLAST
|
Glutamate 5-kinase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
gamma-glutamyl kinase [Escherichia coli CFT073]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
GLUTAMATE_5_KINASE
|
|
MMBR References
|
Nucleic Acids Res. 1984;12:6337-6355
Analysis of the Escherichia coli proBA locus by DNA and protein sequencing.
Deutch, A. H., K. E. Rushlow, and C. J. Smith.
|
J Biol Chem. 1979;254:9429-9440
ilvU, a locus in Escherichia coli affecting the derepression of isoleucyl-tRNA synthetase in the RPC-5 chromatographic profiles of tRNAIle and tRNAVal.
Fayerman, J. T., M. C. Vann, L. S. Williams, and H. E. Umbarger.
|
Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC.
(
6307829
)
|
J Gen Microbiol. 1983;129(10):3215-25.
Sub-cloning of the wild-type proAB region of the Escherichia coli genome.
Hayzer DJ.
(
6317797
)
|
J Gen Microbiol. 1980;118(2):287-93.
The gene-enzyme relationships of proline biosynthesis in Escherichia coli.
Hayzer DJ, Leisinger T.
(
6255065
)
|
J Bacteriol. 1966;91(6):2392
Elective selection of proline-requiring mutants.
Hirota Y, Inuzuka M, Tomoeda M.
(
5329291
)
|
J Bacteriol. 1983;156(3):1249-62.
Genetic analysis of the proBA genes of Salmonella typhimurium: physical and genetic analyses of the cloned proB+ A+ genes of Escherichia coli and of a mutant allele that confers proline overproduction and enhanced osmotolerance.
Mahan MJ, Csonka LN.
(
6315682
)
|
J Bacteriol. 1974;118(3):928-34.
Proline excretion and indirect suppression in Escherichia coli and Salmonella typhimurium.
Berg CM, Rossi JJ.
(
4598010
)
|
Sov. Genet. (Engl. Transl. Genetika) 1986;22:1493-1500.
Cloning of Escherichia coli genes for proline biosynthesis.
Nersisyan, A. A., Y. A. Fedorova, and E. M. Khurges.
|
Biotechnol. Bioeng. 1984;26:74-80.
Proline excretion by Escherichia coli K12.
Rancourt, D. E., J. T. Stephenson, G. A. Vickell, and J. M. Wood.
|
Mol Gen Genet. 1976;147(3):263-9.
Heptose-deficient mutants of Escherichia coli K12 deficient in up to three major outer membrane proteins.
van Alphen W, Lugtenberg B, Berendsen W.
(
787763
)
|
Mol Gen Genet. 1981;182(1):82-6.
Proline over-production results in enhanced osmotolerance in Salmonella typhimurium.
Csonka LN.
(
7022140
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSDSQTLVVK LGTSVLTGGS RRLNRAHIVE LVRQCAQLHA AGHRIVIVTS GAIAAGREHL GYPELPATIA
0071 SKQLLAAVGQ SRLIQLWEQL FSIYGIHVGQ MLLTRADMED RERFLNARDT LRALLDNNIV PVINENDAVA
0141 TAEIKVGDND NLSALAAILA GADKLLLLTD QKGLYTADPR SNPQAELIKD VYGIDDALRA IAGDSVSGLG
0211 TGGMSTKLQA ADVACRAGID TIIAAGSKPG VIGDVMEGIS VGTLFHAQAT PLENRKRWIF GAPPAGEITV
0281 DEGATAAILE RGSSLLPKGI KSVTGNFSRG EVIRICNLEG RDIAHGVSRY NSDALRRIAG HHSQEIDAIL
0351 GYEYGPVAVH RDDMITR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AAAACGCCAT GCTTTGCTCG CAGATGGTTG
-070 GCAACCGACG ACAGTCCTGC TAAAACGTTC GTTTGATATC ATTTTTCCTA AAATTGAATG GCAGAGAATC
0001 atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg cgccgtctga
0071 accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca gttacatgcc gccgggcatc ggattgttat
0141 tgtgacgtcg ggcgcgatcg ccgccggacg tgagcacctg ggttacccgg aactgccagc gaccatcgcc
0211 tcgaaacaac tgctggcggc ggtagggcag agtcgactga ttcaactgtg ggaacagctg ttttcgattt
0281 atggcattca cgtcgggcaa atgctgctga cccgtgctga tatggaagac cgtgaacgct tcctgaacgc
0351 ccgcgacacc ctgcgagcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga tgctgtcgct
0421 acggcagaga ttaaggtcgg cgataacgat aacctttctg cgctggcggc gattcttgcg ggtgccgata
0491 aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgacccgcgc agcaatccgc aggcagaact
0561 gattaaagat gtttacggca ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga
0631 actggcggca tgagtaccaa attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac accattattg
0701 ccgcgggcag caagccgggc gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc
0771 ccaggcgact ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgggtga aatcacggta
0841 gatgaagggg caactgccgc cattctggaa cgcggcagct ccctgttgcc gaaaggcatt aaaagcgtga
0911 ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa cctcgaaggc cgcgatatcg cccacggcgt
0981 cagtcgttac aacagcgatg cattacgccg tattgccgga caccactcgc aagaaattga tgcaatactg
1051 ggatatgaat acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaGGAGCA GGCTGATGCT
1121 GGAACAAATG GGCATTGCCG CGAAGCAAGC CTCGTATAAA TTAGCGCAAC TCTCCAGCCG CGAAAAAAAT
1191 CGCGTGCTGG AAAA
|
