PEC Original Annotations
|
Essentiality
|
Class
|
essential
|
References (PMID)
|
Nucleic Acids Res. 1984;12(12):4977-85.
The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene.
Swindle J, Ajioka J, Dawson D, Myers R, Carroll D, Georgopoulos C.
(
6330693
)
|
|
Deletion
|
OCR39
(D)
|
Related gene (W3110 PEC)
|
Gene Search
|
Search MG1655 PEC by gene name:
nusB
|
Related strains
|
Strains Search
|
Search strains by gene name:
nusB
Search strains by all related name:
nusB b0416 ECK0410 groNB JW0406 ssaD ssyB
|
General information (Go to Linear View:)
|
Gene Name
|
nusB
|
Alternative name
|
b0416,ECK0410,groNB,JW0406,ssaD,ssyB
|
Location, Length
|
434,361 - 434,780
(
+
)
;
9.36
min
;
420
(bp) ,
139
(aa)
|
Product
|
transcription antitermination protein
|
Operon Name
|
nrdR-ribDE-nusB-thiL-pgpA
|
Note
|
transcription termination; L factor; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006350 - transcription
|
Function
|
factor; RNA synthesis, modification, DNA transcription
|
Gene Ontology
|
GO:0003715
;
transcription termination factor activity ( nusB )
GO:0003723
;
RNA binding ( nusB )
GO:0006350
;
transcription ( nusB )
GO:0006353
;
transcription termination ( nusB )
GO:0006355
;
regulation of transcription, DNA-dependent ( nusB )
|
PID
|
1786618
|
EC number
(KEGG Pathway)
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
NUSB_ECOLI
(
P04381
)
|
- Protein name
|
N utilization substance protein B
|
- Synonyms
|
NusB protein
|
- Gene name
|
OrderedLocusNames=b0416, c0527, z0518, ECs0469, SF0353, S0361;
|
Homology Analysis
|
BLAST
|
Bacteria
|
GTOP
|
nusB
(
homologous genes of other bacterias
)
|
PDB
(database updated :
2007.02.20
)
|
PSI-BLAST
|
Chain A, Solution Structure Of Escherichia Coli Nusb
|
SWISS-PROT
(database updated :
2007.02.20
)
|
BLAST
|
N utilization substance protein B (Protein nusB)
|
PSI-BLAST
|
N utilization substance protein B homolog
|
nr
(database updated :
2007.02.20
)
|
BLAST
|
transcription antitermination protein NusB [Escherichia coli O157:H7 EDL933]
|
Pfam 28.0
(database updated :
2015-05
)
|
Pfam
|
|
PROSITE
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
|
MMBR References
|
Virology. 1976;73(1):119-27.
Cooperative effects of bacterial mutations affecting lambda N gene expression. I. Isolation and characterization of a nusB mutant.
Friedman DI, Baumann M, Baron LS.
(
785802
)
|
J Bacteriol. 1987;169(3):1286-90.
secD, a new gene involved in protein export in Escherichia coli.
Gardel C, Benson S, Hunt J, Michaelis S, Beckwith J.
(
3029032
)
|
Mol Gen Genet. 1980;179(1):55-61.
Studies on the E. coli groNB (nusB) gene which affects bacteriophage lambda N gene function.
Georgopoulos CP, Swindle J, Keppel F, Ballivet M, Bisig R, Eisen HA.
(
6161293
)
|
Nucleic Acids Res. 1984;12:4987-4995
Molecular cloning and nucleotide sequencing of the nusB gene of E. coli.
Ishii, S., E. Hatada, T. Maekawa, and F. Imamoto.
|
J Bacteriol. 1985;161(1):285-91.
Identification of five new essential genes involved in the synthesis of a secreted protein in Escherichia coli.
Oliver DB.
(
3881390
)
|
J Bacteriol. 1994;176:4444-4447
ssaD1, a suppressor of secA51(Ts) that renders growth of Escherichia coli cold sensitive, is an early amber mutation in the transcription factor gene nusB.
Rajapandi, T., and D. Oliver.
|
Proc Natl Acad Sci U S A. 1985;82:5275-5279
Defective antitermination of rRNA transcription and derepression of rRNA and tRNA synthesis in the nusB5 mutant of Escherichia coli.
Sharrock, R. A., R. L. Gourse, and M. Nomura.
|
Nucleic Acids Res. 1984;12:4977-4985
The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene.
Swindle, J., J. Ajioka, D. Dawson, R. Myers, D. Carroll, and C. Georgopoulos.
|
Mol Gen Genet. 1992;234(3):429-32.
Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation.
Taura T, Ueguchi C, Shiba K, Ito K.
(
1406588
)
|
Nature. 1981;292(5820):212-5.
The nus mutations affect transcription termination in Escherichia coli.
Ward DF, Gottesman ME.
(
6265784
)
|
J Bacteriol. 1995;177:2589-2591
Structural and functional analyses of the transcription-translation proteins NusB and NusE.
Court, D. L., T. A. Patterson, T. Baker, N. Costantino, X. Mao, and D. I. Friedman.
|
Sequences |
Amino acid
FASTA format
|
0001 MKPAARRRAR ECAVQALYSW QLSQNDIADV EYQFLAEQDV KDVDVLYFRE LLAGVATNTA YLDGLMKPYL
0071 SRLLEELGQV EKAVLRIALY ELSKRSDVPY KVAINEAIEL AKSFGAEDSH KFVNGVLDKA APVIRPNKK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ACCAAAGCTG GCAACAAAGG TGCAGAAGCT
-070 GCACTGACCG CGCTTGAAAT GATTAATGTA TTGAAAGCCA TCAAGGCCTG AAATTAGTAA GGGGAAATCC
0001 gtgaaacctg ctgctcgtcg ccgcgctcgt gagtgtgccg tccaggcgct ctactcctgg cagttgtccc
0071 agaacgacat cgctgatgtt gaataccagt tcctggctga acaggatgta aaagacgttg acgtcctgta
0141 cttccgtgag ctgctggccg gggtggcgac taataccgca tacctcgacg gactgatgaa gccatacctg
0211 tcccgcctgc tggaagaact gggacaggta gaaaaagcag tactgcgcat tgcgctgtac gaactgtcta
0281 aacgtagcga tgtgccatac aaagtggcca ttaacgaagc gatcgaactg gcgaaatcgt tcggcgcaga
0351 agacagccat aagttcgtca acggcgtact cgataaagca gcacctgtga ttcgccctaa caaaaagtga
0421 TATCCAGGCC GGTAGATTCA CGGAAGACCG TTCCATGATC GCCGGCCTTT TCTTTTTTAC CTGCTGAGGC
0491 ATAACGTATG GCATGTGGCG AGTTCTCCCT
|