|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
16-1-2
(LD)
,
DE(sbcB-rfb)202
,
OCL74-2
(D)
,
OCL74-2,75-5 (LD 16-1-2_CRS)
(D)
,
OCL74-2,75-6 (LD 16-1-2_P/O)
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
thiM
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
thiM
Search strains by all related name:
thiM b2104 ECK2097 f262 f262c JW2091
|
General information (Go to Linear View: )
|
|
Gene Name
|
thiM
|
|
Alternative name
|
b2104,ECK2097,f262,f262c,JW2091
|
|
Location, Length
|
2,182,535 - 2,183,323
(
-
)
;
47.04
min
;
789
(bp) ,
262
(aa)
|
|
Product
|
hydoxyethylthiazole kinase
|
|
Operon Name
|
thiMD
|
|
Note
|
GO_process: GO:0009228 - thiamin biosynthetic process
|
|
Function
|
enzyme; Biosynthesis of cofactors, carriers: Thiamin
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( thiM )
GO:0000287
;
magnesium ion binding ( thiM )
GO:0004417
;
hydroxyethylthiazole kinase activity ( thiM )
GO:0005524
;
ATP binding ( thiM )
GO:0009228
;
thiamin biosynthetic process ( thiM )
GO:0016301
;
kinase activity ( thiM )
GO:0016740
;
transferase activity ( thiM )
GO:0046872
;
metal ion binding ( thiM )
|
|
PID
|
1788421
|
EC number
(KEGG Pathway)
|
2.7.1.50
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
THIM_ECOLI
(
P76423
;
Q9S6P4
)
|
|
- Protein name
|
Hydroxyethylthiazole kinase
|
|
- Synonyms
|
EC 2.7.1.50, 4-methyl-5-beta- hydroxyethylthiazole kinase, Thz kinase, TH kinase
|
|
- Gene name
|
Name=thiM; OrderedLocusNames=b2104;
|
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
thiM
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Structure Of Hydroxyethylthiazole Kinase Protein From Pyrococcus Horikoshii Ot3
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Hydroxyethylthiazole kinase
|
|
PSI-BLAST
|
Hydroxyethylthiazole kinase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
hydroxyethylthiazole kinase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
ATP_GTP_A
|
|
MMBR References
|
J Bacteriol. 1989;171(6):3228-32.
The thiM locus and its relation to phosphorylation of hydroxyethylthiazole in Escherichia coli.
Mizote T, Nakayama H.
(
2542220
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MQVDLLGSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM VIETEEASQF AAIASALLIN
0071 VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGIANG
0141 GRGVDTTDAA ANAIPAAQTL ARETGAIVVV TGEMDYVTDG HRIIGIHGGD PLMTKVVGTG CALSAVVAAC
0211 CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL DALWQLTQEV QA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ggctgagaaa tacccgtatc acctgatctg
-070 gataatgcca gcgtagggaa gtcacggacc accaggtcat tgcttcttca cgttatggca ggagcaaact
0001 atgcaagtcg acctgctggg ttcagcgcaa tctgcgcacg cgttacacct ttttcaccaa cattcccctc
0071 ttgtgcactg catgaccaat gatgtggtgc aaacctttac cgccaatacc ttgctggcgc tcggtgcatc
0141 gccagcgatg gttatcgaaa ccgaagaggc cagtcagttt gcggctatcg ccagtgcctt gttgattaac
0211 gttggcacac tgacgcagcc acgcgctcag gcgatgcgtg ctgccgttga gcaagcaaaa agctctcaaa
0281 caccctggac gcttgatcca gtagcggtgg gtgcgctcga ttatcgccgc catttttgtc atgaactttt
0351 atcttttaaa ccggcagcga tacgtggtaa tgcttcggaa atcatggcat tagctggcat tgctaatggc
0421 ggacggggag tggataccac tgacgccgca gctaacgcga tacccgctgc acaaacactg gcacgggaaa
0491 ctggcgcaat cgtcgtggtc actggcgaga tggattatgt taccgatgga catcgtatca ttggtattca
0561 cggtggtgat ccgttaatga ccaaagtggt aggaactggc tgtgcattat cggcggttgt cgctgcctgc
0631 tgtgcgttac caggcgatac gctggaaaat gtcgcatctg cctgtcactg gatgaaacaa gccggagaac
0701 gcgcagtcgc cagaagcgag gggccaggca gttttgttcc acatttcctt gatgcgctct ggcaattgac
0771 gcaggaggtg caggcatgaa acgaattaac gctctgacga ttgccggtac tgatccgagt ggtggtgcgg
0841 ggattcaggc cgatcttaaa accttctcgg cacttggcgc ttatggttg
|
