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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Deletion
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OCL56-20
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
hisS
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Related strains
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Strains Search
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Search strains by gene name:
hisS
Search strains by all related name:
hisS b2514 ECK2510 JW2498
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General information (Go to Linear View: )
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Gene Name
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hisS
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Alternative name
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b2514,ECK2510,JW2498
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Location, Length
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2,637,323 - 2,638,597
(
-
)
;
56.84
min
;
1275
(bp) ,
424
(aa)
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Product
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histidyl tRNA synthetase
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Operon Name
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hisS
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Note
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histidine tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( hisS )
GO:0004812
;
aminoacyl-tRNA ligase activity ( hisS )
GO:0004821
;
histidine-tRNA ligase activity ( hisS )
GO:0005524
;
ATP binding ( hisS )
GO:0005737
;
cytoplasm ( hisS )
GO:0006412
;
translation ( hisS )
GO:0006418
;
tRNA aminoacylation for protein translation ( hisS )
GO:0006427
;
histidyl-tRNA aminoacylation ( hisS )
GO:0016874
;
ligase activity ( hisS )
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PID
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1788861
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EC number
(KEGG Pathway)
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6.1.1.21
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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hisS
(
EG10453
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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SYH_ECOLI
(
P60906
;
P04804
)
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- Protein name
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Histidyl-tRNA synthetase
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- Synonyms
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EC 6.1.1.21, Histidine--tRNA ligase, HisRS
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- Gene name
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Name=hisS; OrderedLocusNames=b2514;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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hisS
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Histidyl-Trna Synthetase Complexed With Histidyl-Adenylate
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Histidyl-tRNA synthetase (Histidine--tRNA ligase) (HisRS)
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PSI-BLAST
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Histidyl-tRNA synthetase
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nr
(database updated :
2007.02.20
)
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BLAST
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histidyl-tRNA synthetase [Escherichia coli O157:H7 EDL933]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AA_TRNA_LIGASE_II_2
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MMBR References
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Mol Gen Genet. 1981;183(1):115-22.
Strains of Escherichia coli carrying the structural gene for histidyl-tRNA synthetase on a high copy-number plasmid.
Eisenbeis SJ, Parker J.
(
6460151
)
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Gene. 1982;18(2):107-14.
The nucleotide sequence of the promoter region of hisS, the structural gene for histidyl-tRNA synthetase.
Eisenbeis SJ, Parker J.
(
6290315
)
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J Biol Chem. 1985;260:10063-10068
Primary structure of histidine-tRNA synthetase and characterization of hisS transcripts.
Freedman, R., B. Gibson, D. Donovan, K. Biemann, S. Eisenbeis, J. Parker, and P. Schimmel.
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| Sequences |
Amino acid
FASTA format
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0001 MAKNIQAIRG MNDYLPGETA IWQRIEGTLK NVLGSYGYSE IRLPIVEQTP LFKRAIGEVT DVVEKEMYTF
0071 EDRNGDSLTL RPEGTAGCVR AGIEHGLLYN QEQRLWYIGP MFRHERPQKG RYRQFHQLGC EVFGLQGPDI
0141 DAELIMLTAR WWRALGISEH VTLELNSIGS LEARANYRDA LVAFLEQHKE KLDEDCKRRM YTNPLRVLDS
0211 KNPEVQALLN DAPALGDYLD EESREHFAGL CKLLESAGIA YTVNQRLVRG LDYYNRTVFE WVTNSLGSQG
0281 TVCAGGRYDG LVEQLGGRAT PAVGFAMGLE RLVLLVQAVN PEFKADPVVD IYLVASGADT QSAAMALAER
0351 LRDELPGVKL MTNHGGGNFK KQFARADKWG ARVAVVLGES EVANGTAVVK DLRSGEQTAV AQDSVAAHLR
0421 TLLG
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 ggaagcgcct cgcttcccgt gtatgattga
-070 acccgcatgg ctcccgaaac attgagggaa gcgttgaggg ttcattttta tattcagaaa gagaataaac
0001 gtggcaaaaa acattcaagc cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc
0071 gcattgaagg cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
0141 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat gtacaccttt
0211 gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg ctgtgtacgc gccggcatcg
0281 agcatggtct tctgtacaat caggaacagc gtctgtggta tatcgggccg atgttccgtc acgagcgtcc
0351 gcagaaaggg cgttatcgtc agttccatca gttgggctgc gaagttttcg gtctgcaagg tccggatatc
0421 gacgctgaac tgattatgct cactgcccgc tggtggcgcg cgctgggtat ttccgagcac gtaactcttg
0491 agctgaactc tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
0561 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cgctgcgcgt gctggattca
0631 aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga ctatctggac gaggaatctc
0701 gtgagcattt tgccggtctg tgcaaactgc tggagagcgc ggggatcgct tacaccgtaa accagcgtct
0771 ggtgcgtggt ctggattact acaaccgtac cgttttcgag tgggtgacta acagtctcgg ctcccagggc
0841 accgtgtgtg caggcggtcg ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg
0911 gttttgctat gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
0981 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt agctgagcgt
1051 ctgcgtgatg aattaccggg cgtgaaattg atgaccaacc acggcggcgg caactttaag aaacagtttg
1121 cccgtgctga taaatggggt gcccgcgttg ctgtggtgct gggtgagtct gaagtggcta acggcacagc
1191 agtagtgaag gatttgcgct ctggtgagca aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc
1261 acgttactgg gttaaggaag gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc
1331 ggttaaacgc ttttttgctg aaaatggcaa agcactggct gttgg
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