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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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J Bacteriol. 1992;174(10):3250-60.
Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12: three operons in the rfa locus.
Roncero C, Casadaban MJ.
(
1577693
)
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Deletion
|
OCL08
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
waaB
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Related strains
|
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Strains Search
|
Search strains by gene name:
waaB
Search strains by all related name:
waaB b3628 ECK3618 JW3603 lps phx* rfaB syn* waaB
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General information (Go to Linear View: )
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Gene Name
|
waaB
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Alternative name
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b3628,ECK3618,JW3603,lps,phx*,rfaB,syn*,waaB
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Location, Length
|
3,801,081 - 3,802,160
(
-
)
;
81.93
min
;
1080
(bp) ,
359
(aa)
|
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Product
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UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase
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Operon Name
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waaQGP-rfaS-waaBIJY-rfaZ-waaK
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Note
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GO_function: GO:0042280 - cell surface antigen activity, host-interacting
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Function
|
enzyme; Surface polysaccharides & antigens
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Gene Ontology
|
GO:0005515
;
protein binding ( rfaB )
GO:0009058
;
biosynthetic process ( rfaB )
GO:0009103
;
lipopolysaccharide biosynthetic process ( rfaB )
GO:0016740
;
transferase activity ( rfaB )
GO:0016757
;
transferase activity, transferring glycosyl groups ( rfaB )
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PID
|
226510984
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EC number
(KEGG Pathway)
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2.4.1.-
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
|
RFAB_ECOLI
(
P27127
)
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- Protein name
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Lipopolysaccharide 1,6-galactosyltransferase
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- Synonyms
|
EC 2.4.1.-, UDP-D- galactose--(Glucosyl)lipopolysaccharide-alpha-1,3-D- galactosyltransferase
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- Gene name
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Name=rfaB; Synonyms=waaB; OrderedLocusNames=b3628;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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rfaB
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Lipopolysaccharide 1,6-galactosyltransferase
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PSI-BLAST
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Glycogen synthase
|
|
nr
(database updated :
2007.02.20
)
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BLAST
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UDP-D-galactose:(glucosyl)lipopolysaccharide-1,6-D-galactosyltransferase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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GLYCOSAMINOGLYCAN
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
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MMBR References
|
J Bacteriol. 1992;174:2525-2538
Role of the rfaG and rfaP genes in determining the lipopolysaccharide core structure and cell surface properties of Escherichia coli K-12.
Parker, C. T., A. W. Kloser, C. A. Schnaitman, M. A. Stein, S. Gottesman, and B. W. Gibson.
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J Bacteriol. 1991;173(20):6428-31.
Effect of rfaH (sfrB) and temperature on expression of rfa genes of Escherichia coli K-12.
Pradel E, Schnaitman CA.
(
1655711
)
|
J Bacteriol. 1992;174:3250-3260
Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12.
Roncero, C., and M. J. Casadaban.
|
Microbiol. Rev. 1993;57:655-682
Genetics of lipopolysaccharide biosynthesis in enteric bacteria.
Schnaitman, C. A., and J. D. Klena.
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Nucleic Acids Res. 1994;22:2576-2586
Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.
Sofia, H. J., V. D. Burland, D. L. Daniels, G. Plunkett, and F. R. Blattner.
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J Biol Chem. 1984;259:3064-3069
Regulation of membrane glycosyltransferases by the sfrB and rfaH genes of Escherichia coli and Salmonella typhimurium.
Creeger, E. S., T. Schulte, and L. I. Rothfield.
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| Sequences |
Amino acid
FASTA format
|
0001 MKIAFIGEAV SGFGGMETVI RDVITTFRQQ HIQSEMFFFC RNDKMDKGWL EGIKYSCSFS NIRLGFLRRA
0071 KHIHALSKWL QEYQPDIVIC IDVISCLFAA KARKKSGIDM PVFSWPHFSL DHKKHAEYIT CADYHLAISS
0141 GIKQQMINRG VAESTINVIF NPVETKDSVI PAPEEGETAT FIYVGRMKFE GQKRVKDLLD GLSQAKGNWK
0211 LHVLGDGSDF EKCQAYGREL NIDDRIVWYG WQQYPWELVQ QDIEKVSALL LTSSFEGFPM TLLEALSWGI
0281 PCISADCVSG PADIIQPDVN GHLYQPGDIA GFVTLLNKYI AGEIHIEHEK IPASIDEFYQ SKYYDRLHKV
0351 IISAISRRK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 aaattcagtc cagataaaat gatgtcaacg
-070 attgtttctg attttataga caaataaaac cgttaaaaca gtgagtttta ctcaattaat gagagtttct
0001 atgaaaatag cgtttatcgg cgaagctgtt tctggatttg gcggcatgga aactgtcatc cgtgatgtta
0071 ttactacctt caggcaacaa catatccaaa gtgaaatgtt tttcttttgt cgtaatgaca aaatggataa
0141 gggatggctg gaaggtatta aatactcatg ttcattttcc aatattcgtt taggtttttt gcgtcgggcg
0211 aaacatattc atgctctaag taaatggctt caggagtacc aaccagatat cgttatttgt attgatgtga
0281 tctcctgcct gtttgccgca aaagcacgta aaaaatcagg aattgatatg ccagtatttt catggccaca
0351 tttttcttta gatcacaaaa aacatgctga atatatcacc tgtgccgact atcatttagc aatcagctct
0421 ggcattaaac agcaaatgat taatcgaggt gtagcagaat cgacgataaa tgttattttt aatcccgtcg
0491 aaactaaaga ctcagtcatt cccgcgcccg aggaaggtga aacggcaacg tttatttatg ttgggcgtat
0561 gaaatttgaa ggccagaaaa gagttaaaga cctgcttgat ggcttatcac aagcgaaagg taactggaaa
0631 ctccatgtat tgggtgatgg ctccgatttc gaaaaatgcc aggcttatgg tagagaatta aatattgatg
0701 atcgaattgt ctggtatggt tggcaacaat atccctggga attggtccag caagatattg aaaaagtgag
0771 tgcgttattg ctcacgtcat cttttgaagg tttcccaatg accctacttg aagccttgtc gtgggggatt
0841 ccttgtatca gtgccgattg tgtctccggg cctgctgaca ttattcaacc tgatgtcaat ggccatctgt
0911 atcaaccggg agatattgct ggttttgtaa ctcttctaaa taaatatata gcaggtgaaa ttcatatcga
0981 acatgaaaaa attcctgcgt cgatcgatga attttaccaa tcgaagtatt acgatagatt acataaggta
1051 attatttctg ctatttcccg gaggaaataa tgcagcaggt gtttttccag gaaactgagt ttcttaattc
1121 agttattgat tatgatcata aagtagaaac agaaaatctt tgtctggaca tcgcttatgg
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