|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
OCL48,49-1
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
recA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
recA
Search strains by all related name:
recA b2699 ECK2694 JW2669 lexB recH rnmB srf tif umuB umuR zab
|
General information (Go to Linear View: )
|
|
Gene Name
|
recA
|
|
Alternative name
|
b2699,ECK2694,JW2669,lexB,recH,rnmB,srf,tif,umuB,umuR,zab
|
|
Location, Length
|
2,820,730 - 2,821,791
(
-
)
;
60.80
min
;
1062
(bp) ,
353
(aa)
|
|
Product
|
DNA strand exchange and recombination protein with protease and nuclease activity
|
|
Operon Name
|
recAX
|
|
Note
|
DNA strand exchange and renaturation, DNA-dependent ATPase, DNA- and ATP-dependent coprotease; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0008233 - peptidase activity; GO_process: GO:0006310 - DNA recombination; GO_process: GO:0006281 - DNA repair; GO_process: GO:0006508 - proteolysis; GO_process: GO:0009432 - SOS response
|
|
Function
|
enzyme; DNA - replication, repair,restriction/modification
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( recA )
GO:0003677
;
DNA binding ( recA )
GO:0003684
;
damaged DNA binding ( recA )
GO:0003697
;
single-stranded DNA binding ( recA )
GO:0005524
;
ATP binding ( recA )
GO:0005737
;
cytoplasm ( recA )
GO:0006259
;
DNA metabolic process ( recA )
GO:0006281
;
DNA repair ( recA )
GO:0006310
;
DNA recombination ( recA )
GO:0006950
;
response to stress ( recA )
GO:0006974
;
response to DNA damage stimulus ( recA )
GO:0008094
;
DNA-dependent ATPase activity ( recA )
GO:0009432
;
SOS response ( recA )
GO:0017111
;
nucleoside-triphosphatase activity ( recA )
|
|
PID
|
1789051
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
recA
(
EG10823
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
RECA_ECOLI
(
P03017
;
P26347; P78213
)
|
|
- Protein name
|
RecA protein
|
|
- Synonyms
|
Recombinase A
|
|
- Gene name
|
OrderedLocusNames=b2699, c3253, z4002, ECs3556, SF2722, S2913;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
recA
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Reca-Adp-Alf4 Complex
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Protein recA (Recombinase A)
|
|
PSI-BLAST
|
Protein recA (Recombinase A)
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
DNA strand exchange and recombination protein with protease and nuclease activity [Escherichia coli APEC O1]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
ATP_GTP_A
RECA
|
|
MMBR References
|
J Bacteriol. 1989;171(5):2415-23.
New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis.
Dutreix M, Moreau PL, Bailone A, Galibert F, Battista JR, Walker GC, Devoret R.
(
2651400
)
|
Trends Genet. 1996;12(1):20-6.
Exchanging partners: recombination in E. coli.
Eggleston AK, West SC.
(
8741856
)
|
J Bacteriol. 1997;179:7435-7445
Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity.
Fijalkowska, I. J., R. L. Dunn, and R. M. Schaaper.
|
J Biol Chem. 1996;271:23874-23883
Interaction of Escherichia coli RecA protein with LexA repressor. II. Inhibition of DNA strand exchange by the uncleavable LexA S119A repressor argues that recombination and SOS induction are competitive processes.
Harmon, F. G., W. M. Rehrauer, and S. C. Kowalczykowski.
|
Proc Natl Acad Sci U S A. 1981;77:313-317.
Organization of the recA gene of Escherichia coli.
Horii, T., T. Ogawa, and H. Ogawa.
|
Mol Gen Genet. 1984;193(2):288-92.
Functional domains of Escherichia coli recA protein deduced from the mutational sites in the gene.
Kawashima H, Horii T, Ogawa T, Ogawa H.
(
6363880
)
|
Mol Gen Genet. 1979;177(1):13-22.
Construction and characterization of a plasmid coding for a fragment of the Escherichia coli recA protein.
Little JW.
(
395410
)
|
Proc Natl Acad Sci U S A. 1981;78:4199-4203
Purified lexA protein is a repressor of the recA and lexA genes.
Little, J. W., D. W. Mount, and C. R. Yanisch-Perron.
|
Proc Natl Acad Sci U S A. 1980;77:2611-2615
Sequences of the recA gene and protein.
Sancar, A., C. Stachelek, W. Konigsberg, and W. D. Rupp.
|
Microbios. 1996;86(346):23-6.
The recA gene and cadmium toxicity in Escherichia coli K12.
Shapiro N, Keasling JD.
(
8771773
)
|
Mol Gen Genet. 1980;179(2):355-8.
Altered regulation of the recA gene in Escherichia coli strains carrying a recA-linked suppressor of lexA.
Thomas A, Lloyd RG.
(
7007810
)
|
Proc Natl Acad Sci U S A. 1981;78:1786-1790
Evidence that rnmB is the operator of the Escherichia coli recA gene.
Volkert, M. R., L. J. Margossian, and A. J. Clark.
|
Mol Gen Genet. 1981;183(3):497-504.
Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions.
Willis DK, Uhlin BE, Amini KS, Clark AJ.
(
6278241
)
|
J Mol Evol. 1997;44(5):528-41.
Evolutionary comparisons of RecA-like proteins across all major kingdoms of living organisms.
Brendel V, Brocchieri L, Sandler SJ, Clark AJ, Karlin S.
(
9115177
)
|
Proc Natl Acad Sci U S A. 1981;78:4204-4208
Mechanism of action of the lexA gene product.
Brent, R., and M. Ptashne.
|
J Bacteriol. 1996;178:2018-2024
Genetic characteristics of new recA mutants of Escherichia coli K-12.
Alexseyev, A. A., I. V. Bakhlanova, E. N. Zaitsev, and V. A. Lanzov.
|
Mutat Res. 1997;381(1):111-5.
Involvement of recA and recF in the induced precise excision of Tn10 in Escherichia coli.
Chan A, Nagel R.
(
9403037
)
|
Biochimie. 1991;73(4):523-32.
rec genes and homologous recombination proteins in Escherichia coli.
Clark AJ.
(
1911953
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MAIDENKQKA LAAALGQIEK QFGKGSIMRL GEDRSMDVET ISTGSLSLDI ALGAGGLPMG RIVEIYGPES
0071 SGKTTLTLQV IAAAQREGKT CAFIDAEHAL DPIYARKLGV DIDNLLCSQP DTGEQALEIC DALARSGAVD
0141 VIVVDSVAAL TPKAEIEGEI GDSHMGLAAR MMSQAMRKLA GNLKQSNTLL IFINQIRMKI GVMFGNPETT
0211 TGGNALKFYA SVRLDIRRIG AVKEGENVVG SETRVKVVKN KIAAPFKQAE FQILYGEGIN FYGELVDLGV
0281 KEKLIEKAGA WYSYKGEKIG QGKANATAWL KDNPETAKEI EKKVRELLLS NPNSTPDFSV DDSEGVAETN
0351 EDF
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 caatttctac aaaacacttg atactgtatg
-070 agcatacagt ataattgctt caacagaaca tattgactat ccggtattac ccggcatgac aggagtaaaa
0001 atggctatcg acgaaaacaa acagaaagcg ttggcggcag cactgggcca gattgagaaa caatttggta
0071 aaggctccat catgcgcctg ggtgaagacc gttccatgga tgtggaaacc atctctaccg gttcgctttc
0141 actggatatc gcgcttgggg caggtggtct gccgatgggc cgtatcgtcg aaatctacgg accggaatct
0211 tccggtaaaa ccacgctgac gctgcaggtg atcgccgcag cgcagcgtga aggtaaaacc tgtgcgttta
0281 tcgatgctga acacgcgctg gacccaatct acgcacgtaa actgggcgtc gatatcgaca acctgctgtg
0351 ctcccagccg gacaccggcg agcaggcact ggaaatctgt gacgccctgg cgcgttctgg cgcagtagac
0421 gttatcgtcg ttgactccgt ggcggcactg acgccgaaag cggaaatcga aggcgaaatc ggcgactctc
0491 acatgggcct tgcggcacgt atgatgagcc aggcgatgcg taagctggcg ggtaacctga agcagtccaa
0561 cacgctgctg atcttcatca accagatccg tatgaaaatt ggtgtgatgt tcggtaaccc ggaaaccact
0631 accggtggta acgcgctgaa attctacgcc tctgttcgtc tcgacatccg tcgtatcggc gcggtgaaag
0701 agggcgaaaa cgtggtgggt agcgaaaccc gcgtgaaagt ggtgaagaac aaaatcgctg cgccgtttaa
0771 acaggctgaa ttccagatcc tctacggcga aggtatcaac ttctacggcg aactggttga cctgggcgta
0841 aaagagaagc tgatcgagaa agcaggcgcg tggtacagct acaaaggtga gaagatcggt cagggtaaag
0911 cgaatgcgac tgcctggctg aaagataacc cggaaaccgc gaaagagatc gagaagaaag tacgtgagtt
0981 gctgctgagc aacccgaact caacgccgga tttctctgta gatgatagcg aaggcgtagc agaaactaac
1051 gaagattttt aatcgtcttg tttgatacac aagggtcgca tctgcggccc ttttgctttt ttaagttgta
1121 aggatatgcc atgacagaat caacatcccg tcgcccggca ta
|
