MG1655
W3110
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Gene Report : manX
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL82 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  manX
Related strains
     Strains Search Search strains by gene name:  manX   Search strains by all related name:  manX b1817 ECK1815 gptB JW1806 mpt ptsL ptsM ptsX

General information  (Go to Linear View:)
 Gene Name manX  
 Alternative name b1817,ECK1815,gptB,JW1806,mpt,ptsL,ptsM,ptsX  
 Location, Length 1,900,072 - 1,901,043 (  +  ) ;   40.95 min ; 972 (bp) ,   323 (aa) Go to Linear View
 Product fused mannose-specific PTS enzymes: IIAcomponent/IIB component  
 Operon Name manXYZ  
 Note PTS enzyme IIAB, mannose-specific; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0005351 ; sugar:hydrogen ion symporter activity ( manX )
GO:0005515 ; protein binding ( manX )
GO:0005737 ; cytoplasm ( manX )
GO:0005886 ; plasma membrane ( manX )
GO:0006810 ; transport ( manX )
GO:0008643 ; carbohydrate transport ( manX )
GO:0008982 ; protein-N(PI)-phosphohistidine-sugar phosphotransferase activity ( manX )
GO:0009401 ; phosphoenolpyruvate-dependent sugar phosphotransferase system ( manX )
GO:0016020 ; membrane ( manX )
GO:0016021 ; integral to membrane ( manX )
GO:0016740 ; transferase activity ( manX )
 PID 1788120  
 EC number
  (KEGG Pathway)   		2.7.1.69  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene manX ( EG10567 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) PTNA_ECOLI ( P08186 ; Q47350 )
    -  Protein name PTS system, mannose-specific IIAB component  
    -  Synonyms EIIAB-Man, Mannose- permease IIAB component, Phosphotransferase enzyme II, AB component, EC 2.7.1.69, EIII-Man  
    -  Gene name OrderedLocusNames=b1817, c2223, z2860, ECs2527, SF1411, S1526;  

 Linear View (Whole Mode)
View Location
   1890.0  –  1915.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP manX (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of The Iibsor Domain Of The Sorbose Permease From Klebsiella Pneumoniae Solved To 1.75a Resolution  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST PTS system mannose-specific EIIAB component  
         PSI-BLAST PTS system mannose-specific EIIAB component
    nr     (database updated : 2007.02.20 )
         BLAST PTS system, mannose-specific IIAB component [Escherichia coli UTI89]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    CRYSTALLIN_BETAGAMMA     

Other Cross-Reference
    COG COG2893G 
    EcoCyc manX 

MMBR References
J Biol Chem. 1985;260:15495-15503
The mannose-permease of the bacterial phosphotransferase system. Gene cloning and purification of the enzyme IIMan/IIIMan complex of Escherichia coli.
Erni, B., and B. Zanolari.
J Biol Chem. 1987;262:5238-5247
The mannose permease of Escherichia coli consists of three different proteins: amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA.
Erni, B., B. Zanolari, and H. P. Kocher.
Mol Gen Genet. 1985;199(3):427-33.
Gene fusions to the ptsM/pel locus of Escherichia coli.
Palva ET, Saris P, Silhavy TJ. ( 3162078 )
Proc Natl Acad Sci U S A. 1986;83:8934-8938
Pel, the protein that permits lambda DNA penetration of Escherichia coli, is encoded by a gene in ptsM and is required for mannose utilization by the phosphotransferase system.
Williams, N., D. K. Fox, C. Shea, and S. Roseman.

Sequences
Amino acid
FASTA format 
0001 MTIAIVIGTH GWAAEQLLKT AEMLLGEQEN VGWIDFVPGE NAETLIEKYN AQLAKLDTTK GVLFLVDTWG 
0071 GSPFNAASRI VVDKEHYEVI AGVNIPMLVE TLMARDDDPS FDELVALAVE TGREGVKALK AKPVEKAAPA 
0141 PAAAAPKAAP TPAKPMGPND YMVIGLARID DRLIHGQVAT RWTKETNVSR IIVVSDEVAA DTVRKTLLTQ 
0211 VAPPGVTAHV VDVAKMIRVY NNPKYAGERV MLLFTNPTDV ERLVEGGVKI TSVNVGGMAF RQGKTQVNNA 
0281 VSVDEKDIEA FKKLNARGIE LEVRKVSTDP KLKMMDLISK IDK
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             TTTTGGTTGT AGCCCTTATC TGAATCGATT 
-070 CGATTGTGGA CGACGATTCA AAAATACATC TGGCACGTTG AGGTGTTAAC GATAATAAAG GAGGTAGCAA 
0001 gtgaccattg ctattgttat aggcacacat ggttgggctg cagagcagtt gcttaaaacg gcagaaatgc 
0071 tgttaggcga gcaggaaaac gtcggctgga tcgatttcgt tccaggtgaa aatgccgaaa cgctgattga 
0141 aaagtacaac gctcagttgg caaaactcga caccactaaa ggcgtgctgt ttctcgttga tacatgggga 
0211 ggcagcccgt tcaatgctgc cagccgcatt gtcgtcgaca aagagcatta tgaagtcatt gcaggcgtta 
0281 acattccaat gctcgtggaa acgttaatgg cccgtgatga tgacccaagc tttgatgaac tggtggcact 
0351 ggcagtagaa acaggccgtg aaggcgtgaa agcactgaaa gccaaaccgg ttgaaaaagc cgcgccagca 
0421 cccgctgccg cagcaccaaa agcggctcca actccggcaa aaccaatggg gccaaacgac tacatggtta 
0491 ttggccttgc gcgtatcgac gaccgtctga ttcacggtca ggtcgccacc cgctggacca aagaaaccaa 
0561 tgtctcccgt attattgttg ttagtgatga agtggctgcg gataccgttc gtaagacact gctcacccag 
0631 gttgcacctc cgggcgtaac agcacacgta gttgatgttg ccaaaatgat tcgcgtctac aacaacccga 
0701 aatatgctgg cgaacgcgta atgctgttat ttaccaaccc aacagatgta gagcgtctcg ttgaaggcgg 
0771 cgtgaaaatc acctctgtta acgtcggtgg tatggcattc cgtcagggta aaacccaggt gaataacgcg 
0841 gtttcggttg atgaaaaaga tatcgaggcg ttcaagaaac tgaatgcgcg cggtattgag ctggaagtcc 
0911 gtaaggtttc caccgatccg aaactgaaaa tgatggatct gatcagcaaa atcgataagt aaCGTATTGT 
0981 GTTGATTATC ACTCAGTTTT CACACTTAAG TCTTACGTAA ACAGGAGAAG TACAATGGAG ATTACCACTC 
1051 TTCAAATTGT GCTGGTATTT AT