|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
FEMS Microbiol Lett. 1993;114(3):349-54.
Complementation of growth defect in an ampC deletion mutant of Escherichia coli.
Bishop RE, Weiner JH.
(
8288112
)
|
|
|
Deletion
|
OCR16-2
(D)
,
OCR16-4
(D)
,
OCR16-5
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
ampC
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
ampC
Search strains by all related name:
ampC ampA b4150 ECK4146 JW4111
|
General information (Go to Linear View: )
|
|
Gene Name
|
ampC
|
|
Alternative name
|
ampA,b4150,ECK4146,JW4111
|
|
Location, Length
|
4,375,834 - 4,376,967
(
-
)
;
94.31
min
;
1134
(bp) ,
377
(aa)
|
|
Product
|
beta-lactamase/D-alanine carboxypeptidase
|
|
Operon Name
|
ampC
|
|
Note
|
beta-lactamase; penicillin resistance; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0042493 - response to drug
|
|
Function
|
enzyme; Drug/analog sensitivity
|
|
Gene Ontology
|
GO:0005515
;
protein binding ( ampC )
GO:0008800
;
beta-lactamase activity ( ampC )
GO:0016787
;
hydrolase activity ( ampC )
GO:0017001
;
antibiotic catabolic process ( ampC )
GO:0030288
;
outer membrane-bounded periplasmic space ( ampC )
GO:0030655
;
beta-lactam antibiotic catabolic process ( ampC )
GO:0042597
;
periplasmic space ( ampC )
GO:0046677
;
response to antibiotic ( ampC )
|
|
PID
|
1790593
|
EC number
(KEGG Pathway)
|
3.5.2.6
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
ampC
(
EG10040
)
|
|
Number of removed
|
19 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
AMPC_ECOLI
(
P00811
)
|
|
- Protein name
|
Beta-lactamase precursor
|
|
- Synonyms
|
EC 3.5.2.6, Cephalosporinase
|
|
- Gene name
|
Name=ampC; Synonyms=ampA; OrderedLocusNames=b4150;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
ampC
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Ampc Beta-Lactamase From Escherichia Coli
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Beta-lactamase precursor
|
|
PSI-BLAST
|
Alanyl-tRNA synthetase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
beta-lactamase/D-alanine carboxypeptidase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
BETA_LACTAMASE_C
|
|
MMBR References
|
Nature. 1981;292(5820):269-71.
Recombination between short DNA homologies causes tandem duplication.
Edlund T, Normark S.
(
7019717
)
|
Mol Gen Genet. 1979;173(2):115-25.
Isolation and characterization of DNA repetitions carrying the chromosomal beta-lactamase gene of Escherichia coli K-12.
Edlund T, Grundstrom T, Normark S.
(
386030
)
|
J Bacteriol. 1965;90:54-62.
Resistance of Escherichia coli to penicillins. I. Genetic study of some ampicillin mutants.
Eriksson-Grennberg, K. G., H. G. Boman, J. A. T. Jansson, and S. Thoren.
|
Proc Natl Acad Sci U S A. 1982;79:1111-1115
Overlap between ampC and frd operons on the Escherichia coli chromosome.
Grundstrom, T., and B. Jaurin.
|
J Bacteriol. 1980;143(3):1127-34.
Physical mapping and expression of hybrid plasmids carrying chromosomal beta-lactamase genes of Escherichia coli K-12.
Grundstrom T, Jaurin B, Edlund T, Normark S.
(
6251026
)
|
J Bacteriol. 1997;179:6112-6121
AmpC and AmpH, proteins related to the class C beta-lactamases, bind penicillin and contribute to the normal morphology of Escherichia coli.
Henderson, T. A., K. D. Young, S. A. Denome, and P. K. Elf.
|
Proc Natl Acad Sci U S A. 1981;78:4897-4901
ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of -lactamase of the penicillinase type.
Jaurin, B., and T. Grundstrom.
|
EMBO J. 1982;1(7):875-81.
Sequence elements determining ampC promoter strength in E. coli.
Jaurin B, Grundstrom T, Normark S.
(
6329713
)
|
Nature. 1981;290(5803):221-5.
The E. coli beta-lactamase attenuator mediates growth rate-dependent regulation.
Jaurin B, Grundstrom T, Edlund T, Normark S.
(
7010184
)
|
J Bacteriol. 1977;132(3):912-22.
Escherichia coli K-12 mutants hyperproducing chromosomal beta-lactamase by gene repetitions.
Normark S, Edlund T, Grundstrom T, Bergstrom S, Wolf-Watz H.
(
336612
)
|
Eur J Biochem. 1979;102:65-71
Production of a soluble form of fumarate reductase by multiple gene duplications in Escherichia coli K12.
Cole, S. T., and J. R. Guest.
|
| Sequences |
Amino acid
FASTA format
|
0001 MFKTTLCALL ITASCSTFAA PQQINDIVHR TITPLIEQQK IPGMAVAVIY QGKPYYFTWG YADIAKKQPV
0071 TQQTLFELGS VSKTFTGVLG GDAIARGEIK LSDPTTKYWP ELTAKQWNGI TLLHLATYTA GGLPLQVPDE
0141 VKSSSDLLRF YQNWQPAWAP GTQRLYANSS IGLFGALAVK PSGLSFEQAM QTRVFQPLKL NHTWINVPPA
0211 EEKNYAWGYR EGKAVHVSPG ALDAEAYGVK STIEDMARWV QSNLKPLDIN EKTLQQGIQL AQSRYWQTGD
0281 MYQGLGWEML DWPVNPDSII NGSDNKIALA ARPVKAITPP TPAVRASWVH KTGATGGFGS YVAFIPEKEL
0351 GIVMLANKNY PNPARVDAAW QILNALQ
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tgacagttgt cacgctgatt ggtgtcgtta
-070 caatctaacg catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct
0001 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc cctcaacaaa
0071 tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atcccgggta tggcggtggc
0141 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc
0211 acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttactgg cgtgcttggt ggcgacgcta
0281 ttgctcgagg ggaaatcaag ttaagcgatc ccacaacaaa atactggcct gaacttaccg ctaaacagtg
0351 gaatgggatc acactattac atctcgcaac ctacactgct ggcggcctgc cattgcaggt gccggatgag
0421 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac
0491 gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
0561 gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt accgcccgca
0631 gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt ttcgcctggg gcgttagatg
0701 ctgaagctta tggtgtgaag tcgaccattg aagatatggc ccgctgggtg caaagcaatt taaaacccct
0771 tgatatcaat gagaaaacgc ttcaacaagg gatacaactg gcacaatctc gctactggca aaccggcgat
0841 atgtatcagg gcctgggctg ggaaatgctg gactggccgg taaatcctga cagcatcatt aacggcagtg
0911 acaataaaat tgcactggca gcacgccccg taaaagcgat tacgccccca actcctgcag tacgcgcatc
0981 atgggtacat aaaacagggg cgaccggcgg atttggtagc tatgtcgcgt ttattccaga aaaagagctg
1051 ggtatcgtga tgctggcaaa caaaaactat cccaatccag cgagagtcga cgccgcctgg cagattctta
1121 acgctctaca gtaaaattcc atcgggtccg aattttcgga ccttttctcc gcttttcctt gctgtcatct
1191 acacttagaa aaaaaccagt aaggaaacat ttatgcgcct gctc
|
