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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Nature. 1974;250(5466):513-4.
Replication of Escherichia coli requires DNA polymerase I.
Olivera RM, Bonhoeffer E.
(
4620020
)
|
J Bacteriol. 1984;158:636-643
Method for determining whether a gene of Escherichia coli is essential: Application to the polA gene.
C. M. Joyce and N. D. F. Grindley.
(
6233260
)
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Deletion
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OCR05,6-2
(D)
,
OCR06-18 (AK5-15 / BK6-15)
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
polA
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Related strains
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Strains Search
|
Search strains by gene name:
polA
Search strains by all related name:
polA b2651 b3863 ECK3855 JW3835 o43 resA
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General information (Go to Linear View: )
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Gene Name
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polA
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Alternative name
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b2651,b3863,ECK3855,JW3835,o43,resA
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Location, Length
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4,044,989 - 4,047,775
(
+
)
;
87.18
min
;
2787
(bp) ,
928
(aa)
|
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Product
|
fused DNA polymerase I 5'->3' polymerase/3'->5'exonuclease/5'->3' exonuclease
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Operon Name
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polA
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Note
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DNA polymerase I, 3' --> 5' polymerase, 5' --> 3' and 3' --> 5' exonuclease; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0003676
;
nucleic acid binding ( polA )
GO:0003677
;
DNA binding ( polA )
GO:0003824
;
catalytic activity ( polA )
GO:0003887
;
DNA-directed DNA polymerase activity ( polA )
GO:0004518
;
nuclease activity ( polA )
GO:0004527
;
exonuclease activity ( polA )
GO:0005515
;
protein binding ( polA )
GO:0005622
;
intracellular ( polA )
GO:0005634
;
nucleus ( polA )
GO:0005737
;
cytoplasm ( polA )
GO:0006139
;
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process ( polA )
GO:0006260
;
DNA replication ( polA )
GO:0006281
;
DNA repair ( polA )
GO:0006974
;
response to DNA damage stimulus ( polA )
GO:0008408
;
3'-5' exonuclease activity ( polA )
GO:0008409
;
5'-3' exonuclease activity ( polA )
GO:0016740
;
transferase activity ( polA )
GO:0016779
;
nucleotidyltransferase activity ( polA )
GO:0016787
;
hydrolase activity ( polA )
GO:0043565
;
sequence-specific DNA binding ( polA )
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PID
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1790294
|
EC number
(KEGG Pathway)
|
2.7.7.7
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Homology Analysis
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BLAST
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|
Bacteria
|
|
GTOP
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polA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain A, All-Oxygen Dna Complexed To The 3'-5' Exonuclease Of Dna Polymerase I From E. Coli
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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DNA polymerase I
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PSI-BLAST
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DNA polymerase I
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nr
(database updated :
2007.02.20
)
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BLAST
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DNA polymerase I [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
DNA_POLYMERASE_A
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MMBR References
|
J Biol Chem. 1982;257:1958-1964
Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.
Joyce, C. M., W. S. Kelley, and N. Grindley.
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Genetics 1980;95:15-38
Mapping of the polA locus of Escherichia coli K12: genetic fine structure of the cistron.
Kelley, W. S.
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Nature. 1971;230(5288):33-6.
Purification of an altered DNA polymerase from an E. coli strain with a pol mutation.
Kelley WS, Whitfield HJ.
(
4930962
)
|
Mol Gen Genet. 1979;175(1):77-87.
Characterization of lambda polA transducing phages; effective expression of the E. coli polA gene.
Murray NE, Kelley WS.
(
159999
)
|
J Bacteriol. 1993;175:428-437
Suppression of ColE1 high-copy-number mutants by mutations in the polA gene of Escherichia coli.
Yang, Y.-L., and B. Polisky.
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Nucleic Acids Res. 1995;23:4620-4627
DNA strand transfer catalyzed by the 5'-3' exonuclease domain of Escherichia coli DNA polymerase I.
Zhang, W., and D. H. Evans.
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In A. D. Hershey (ed.), The bacteriophage lambda. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. 1971:455-468
The role of recombination in growth of bacteriophage lambda. I. The gamma gene.
Zissler, J., E. Signer, and F. Schaefer.
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| Sequences |
Amino acid
FASTA format
|
0001 MVQIPQNPLI LVDGSSYLYR AYHAFPPLTN SAGEPTGAMY GVLNMLRSLI MQYKPTHAAV VFDAKGKTFR
0071 DELFEHYKSH RPPMPDDLRA QIEPLHAMVK AMGLPLLAVS GVEADDVIGT LAREAEKAGR PVLISTGDKD
0141 MAQLVTPNIT LINTMTNTIL GPEEVVNKYG VPPELIIDFL ALMGDSSDNI PGVPGVGEKT AQALLQGLGG
0211 LDTLYAEPEK IAGLSFRGAK TMAAKLEQNK EVAYLSYQLA TIKTDVELEL TCEQLEVQQP AAEELLGLFK
0281 KYEFKRWTAD VEAGKWLQAK GAKPAAKPQE TSVADEAPEV TATVISYDNY VTILDEETLK AWIAKLEKAP
0351 VFAFDTETDS LDNISANLVG LSFAIEPGVA AYIPVAHDYL DAPDQISRER ALELLKPLLE DEKALKVGQN
0421 LKYDRGILAN YGIELRGIAF DTMLESYILN SVAGRHDMDS LAERWLKHKT ITFEEIAGKG KNQLTFNQIA
0491 LEEAGRYAAE DADVTLQLHL KMWPDLQKHK GPLNVFENIE MPLVPVLSRI ERNGVKIDPK VLHNHSEELT
0561 LRLAELEKKA HEIAGEEFNL SSTKQLQTIL FEKQGIKPLK KTPGGAPSTS EEVLEELALD YPLPKVILEY
0631 RGLAKLKSTY TDKLPLMINP KTGRVHTSYH QAVTATGRLS STDPNLQNIP VRNEEGRRIR QAFIAPEDYV
0701 IVSADYSQIE LRIMAHLSRD KGLLTAFAEG KDIHRATAAE VFGLPLETVT SEQRRSAKAI NFGLIYGMSA
0771 FGLARQLNIP RKEAQKYMDL YFERYPGVLE YMERTRAQAK EQGYVETLDG RRLYLPDIKS SNGARRAAAE
0841 RAAINAPMQG TAADIIKRAM IAVDAWLQAE QPRVRMIMQV HDELVFEVHK DDVDAVAKQI HQLMENCTRL
0911 DVPLLVEVGS GENWDQAH
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AGATCTACAA AAGATTTTCA CGAAAAGCGG
-070 TGAAAAACTC ATGTTTTCAT CCTGTCTGTG GCATCCTTTA CCCATAATCT GATAAACAGG CACGGACATT
0001 atggttcaga tcccccaaaa tccacttatc cttgtagatg gttcatctta tctttatcgc gcatatcacg
0071 cgtttccccc gctgactaac agcgcaggcg agccgaccgg tgcgatgtat ggtgtcctca acatgctgcg
0141 cagtctgatc atgcaatata aaccgacgca tgcagcggtg gtctttgacg ccaagggaaa aacctttcgt
0211 gatgaactgt ttgaacatta caaatcacat cgcccgccaa tgccggacga tctgcgtgca caaatcgaac
0281 ccttgcacgc gatggttaaa gcgatgggac tgccgctgct ggcggtttct ggcgtagaag cggacgacgt
0351 tatcggtact ctggcgcgcg aagccgaaaa agccgggcgt ccggtgctga tcagcactgg cgataaagat
0421 atggcgcagc tggtgacgcc aaatattacg cttatcaata ccatgacgaa taccatcctc ggaccggaag
0491 aggtggtgaa taagtacggc gtgccgccag aactgatcat cgatttcctg gcgctgatgg gtgactcctc
0561 tgataacatt cctggcgtac cgggcgtcgg tgaaaaaacc gcgcaggcat tgctgcaagg tcttggcgga
0631 ctggatacgc tgtatgccga gccagaaaaa attgctgggt tgagcttccg tggcgcgaaa acaatggcag
0701 cgaagctcga gcaaaacaaa gaagttgctt atctctcata ccagctggcg acgattaaaa ccgacgttga
0771 actggagctg acctgtgaac aactggaagt gcagcaaccg gcagcggaag agttgttggg gctgttcaaa
0841 aagtatgagt tcaaacgctg gactgctgat gtcgaagcgg gcaaatggtt acaggccaaa ggggcaaaac
0911 cagccgcgaa gccacaggaa accagtgttg cagacgaagc accagaagtg acggcaacgg tgatttctta
0981 tgacaactac gtcaccatcc ttgatgaaga aacactgaaa gcgtggattg cgaagctgga aaaagcgccg
1051 gtatttgcat ttgataccga aaccgacagc cttgataaca tctctgctaa cctggtcggg ctttcttttg
1121 ctatcgagcc aggcgtagcg gcatatattc cggttgctca tgattatctt gatgcgcccg atcaaatctc
1191 tcgcgagcgt gcactcgagt tgctaaaacc gctgctggaa gatgaaaagg cgctgaaggt cgggcaaaac
1261 ctgaaatacg atcgcggtat tctggcgaac tacggcattg aactgcgtgg gattgcgttt gataccatgc
1331 tggagtccta cattctcaat agcgttgccg ggcgtcacga tatggacagc ctcgcggaac gttggttgaa
1401 gcacaaaacc atcacttttg aagagattgc tggtaaaggc aaaaatcaac tgacctttaa ccagattgcc
1471 ctcgaagaag ccggacgtta cgccgccgaa gatgcagatg tcaccttgca gttgcatctg aaaatgtggc
1541 cggatctgca aaaacacaaa gggccgttga acgtcttcga gaatatcgaa atgccgctgg tgccggtgct
1611 ttcacgcatt gaacgtaacg gtgtgaagat cgatccgaaa gtgctgcaca atcattctga agagctcacc
1681 cttcgtctgg ctgagctgga aaagaaagcg catgaaattg caggtgagga atttaacctt tcttccacca
1751 agcagttaca aaccattctc tttgaaaaac agggcattaa accgctgaag aaaacgccgg gtggcgcgcc
1821 gtcaacgtcg gaagaggtac tggaagaact ggcgctggac tatccgttgc caaaagtgat tctggagtat
1891 cgtggtctgg cgaagctgaa atcgacctac accgacaagc tgccgctgat gatcaacccg aaaaccgggc
1961 gtgtgcatac ctcttatcac caggcagtaa ctgcaacggg acgtttatcg tcaaccgatc ctaacctgca
2031 aaacattccg gtgcgtaacg aagaaggtcg tcgtatccgc caggcgttta ttgcgccaga ggattatgtg
2101 attgtctcag cggactactc gcagattgaa ctgcgcatta tggcgcatct ttcgcgtgac aaaggcttgc
2171 tgaccgcatt cgcggaagga aaagatatcc accgggcaac ggcggcagaa gtgtttggtt tgccactgga
2241 aaccgtcacc agcgagcaac gccgtagcgc gaaagcgatc aactttggtc tgatttatgg catgagtgct
2311 ttcggtctgg cgcggcaatt gaacattcca cgtaaagaag cgcagaagta catggacctt tacttcgaac
2381 gctaccctgg cgtgctggag tatatggaac gcacccgtgc tcaggcgaaa gagcagggct acgttgaaac
2451 gctggacgga cgccgtctgt atctgccgga tatcaaatcc agcaatggtg ctcgtcgtgc agcggctgaa
2521 cgtgcagcca ttaacgcgcc aatgcaggga accgccgccg acattatcaa acgggcgatg attgccgttg
2591 atgcgtggtt acaggctgag caaccgcgtg tacgtatgat catgcaggta cacgatgaac tggtatttga
2661 agttcataaa gatgatgttg atgccgtcgc gaagcagatt catcaactga tggaaaactg tacccgtctg
2731 gatgtgccgt tgctggtgga agtggggagt ggcgaaaact gggatcaggc gcactaaGAT TCGCCTGAAC
2801 ATGCCTTTTT TCGTAAGTAA GCAACATAAG CTGTCACGTT TTGTGATGGC TATTAGAAAT TCCTATGCAA
2871 CAACTGAAAA AAAATTA
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