MG1655
W3110
Search for
Japanese | English
Gene Report : tonB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR68 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  tonB
Related strains
     Strains Search Search strains by gene name:  tonB   Search strains by all related name:  tonB b1252 ECK1246 exbA JW5195 T1rec

General information  (Go to Linear View:)
 Gene Name tonB  
 Alternative name b1252,ECK1246,exbA,JW5195,T1rec  
 Location, Length 1,309,113 - 1,309,832 (  +  ) ;   28.22 min ; 720 (bp) ,   239 (aa) Go to Linear View
 Product membrane spanning protein in TonB-ExbB-ExbD transport complex  
 Operon Name tonB  
 Note energy transducer; uptake of iron, cyanocobalimin; sensitivity to phages, colicins; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane  
 Function membrane; Transport of small molecules: Cations  
 Gene Ontology GO:0005381 ; iron ion transmembrane transporter activity ( tonB )
GO:0005886 ; plasma membrane ( tonB )
GO:0006810 ; transport ( tonB )
GO:0006826 ; iron ion transport ( tonB )
GO:0008565 ; protein transporter activity ( tonB )
GO:0009597 ; detection of virus ( tonB )
GO:0015031 ; protein transport ( tonB )
GO:0016020 ; membrane ( tonB )
GO:0016021 ; integral to membrane ( tonB )
GO:0030288 ; outer membrane-bounded periplasmic space ( tonB )
GO:0042597 ; periplasmic space ( tonB )
GO:0043213 ; bacteriocin transport ( tonB )
GO:0046718 ; entry of virus into host cell ( tonB )
 PID 1787505  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) TONB_ECOLI ( P94739 ; P97239 )
    -  Protein name TonB protein.  
    -  Synonyms  
    -  Gene name Name=tonB; Synonyms=exbA; OrderedLocusNames=b1252;  

 Linear View (Whole Mode)
View Location
   1300.0  –  1325.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP tonB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain C, Crystal Structure Of Tonb In Complex With Fhua, E. Coli Outer Membrane Receptor For Ferrichrome  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Protein tonB  
         PSI-BLAST Cortactin-binding protein 2
    nr     (database updated : 2007.02.20 )
         BLAST membrane protein (tonB)  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0810M 
    EcoCyc tonB 

MMBR References
J Bacteriol. 1989;171(9):5127-34.
Involvement of ExbB and TonB in transport across the outer membrane of Escherichia coli: phenotypic complementation of exb mutants by overexpressed tonB and physical stabilization of TonB by ExbB.
Fischer E, Gunter K, Braun V. ( 2670904 )
J Mol Biol. 1969;44(1):117-27.
Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.
Gottesman S, Beckwith JR. ( 4897798 )
Biken J. 1966;9(2):77-87.
Studies on defective lysogeny due to chromosomal deletion in Escherichia coli. I. Single lysogens.
Gratia JP. ( 5330517 )
J Bacteriol. 1995;177:4829-4835
Mutual inhibition of cobalamin and siderophore uptake systems suggests their competition for TonB function.
Kadner, R. J., and K. Heller.
Mol Microbiol. 1993;8(2):379-88.
A sequence-specific function for the N-terminal signal-like sequence of the TonB protein.
Karlsson M, Hannavy K, Higgins CF. ( 8316087 )
Mol Microbiol. 1993;8(2):389-96.
ExbB acts as a chaperone-like protein to stabilize TonB in the cytoplasm.
Karlsson M, Hannavy K, Higgins CF. ( 8100348 )
FEMS Microbiol Lett. 1994;119(1-2):71-6.
Energy-dependent receptor activities of Escherichia coli K-12: mutated TonB proteins alter FhuA receptor activities to phages T5, T1, phi 80 and to colicin M.
Killmann H, Braun V. ( 8039674 )
Mol Microbiol. 1997;24(2):271-83.
TonB protein appears to transduce energy by shuttling between the cytoplasmic membrane and the outer membrane in Escherichia coli.
Letain TE, Postle K. ( 9159515 )
J Bacteriol. 1976;128(1):242-7.
Transport of vitamin B12 in tonB mutants of Escherichia coli.
Bassford PJ Jr, Bradbeer C, Kadner RJ, Schnaitman CA. ( 135755 )
Proc Natl Acad Sci U S A. 1983;80:5235-5239
DNA sequence of the Escherichia coli tonB gene.
Postle, K., and R. F. Good.
Cell. 1985;41(2):577-85.
A bidirectional rho-independent transcription terminator between the E. coli tonB gene and an opposing gene.
Postle K, Good RF. ( 2985285 )
J Mol Biol. 1979;131(3):619-36.
Identification of the Escherichia coli tonB gene product in minicells containing tonB hybrid plasmids.
Postle K, Reznikoff WS. ( 390162 )
J Mol Biol. 1965;15:243-255.
Interaction of prophages at the att80 site with the chromosome of Escherichia coli.
Signer, E.
Mol Microbiol. 1993;8(2):409-23.
Activity domains of the TonB protein.
Traub I, Gaisser S, Braun V. ( 8316089 )
FEMS Microbiol Lett. 1994;119(1-2):65-70.
Energy-coupled colicin transport through the outer membrane of Escherichia coli K-12: mutated TonB proteins alter receptor activities and colicin uptake.
Traub I, Braun V. ( 8039672 )
Mol Microbiol. 1993;8(2):261-8.
Evolutionary relationship of uptake systems for biopolymers in Escherichia coli: cross-complementation between the TonB-ExbB-ExbD and the TolA-TolQ-TolR proteins.
Braun V, Herrmann C. ( 8316079 )
J Bacteriol. 1980;142(1):162-8.
Penetration of colicin M into cells of Escherichia coli.
Braun V, Frenz J, Hantke K, Schaller H. ( 6989802 )

Sequences
Amino acid
FASTA format
0001 MTLDLPRRFP WPTLLSVCIH GAVVAGLLYT SVHQVIELPA PAQPISVTMV TPADLEPPQA VQPPPEPVVE 
0071 PEPEPEPIPE PPKEAPVVIE KPKPKPKPKP KPVKKVQEQP KRDVKPVESR PASPFENTAP ARLTSSTATA 
0141 ATSKPVTSVA SGPRALSRNQ PQYPARAQAL RIEGQVKVKF DVTPDGRVDN VQILSAKPAN MFEREVKNAM 
0211 RRWRYEPGKP GSGIVVNILF KINGTTEIQ

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             ACATTTTCAC TGATCCTGAT CGTCTTGCCT 
-070 TATTGAATAT GATTGCTATT TGCATTTAAA ATCGAGACCT GGTTTTTCTA CTGAAATGAT TATGACTTCA 
0001 atgacccttg atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat ggtgctgttg 
0071 tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg cctgcgcagc cgatttctgt 
0141 cacgatggtt acgcctgctg atctcgaacc gccacaagcc gttcagccgc caccggagcc ggtggtagag 
0211 ccagaaccgg aacctgagcc gatccccgaa ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc 
0281 cgaaacctaa gccaaaaccg aagccggtga aaaaggtaca ggagcagcca aaacgcgatg tcaaacccgt 
0351 agagtcgcgt ccggcatcac cgtttgaaaa tacggcaccg gcacgcctga catcaagtac agcaacggct 
0421 gcaaccagca agccggttac cagtgtggct tcaggaccac gcgcattaag ccgtaatcag ccgcagtatc 
0491 cggcacgagc acaggcattg cgcattgaag ggcaggttaa agttaaattt gacgttacgc cggatggtcg 
0561 cgtggataac gtacaaatcc tctcagccaa gcctgcgaac atgtttgagc gtgaggtgaa aaatgcgatg 
0631 cgcagatggc gttatgagcc gggtaagcca ggcagtggga ttgtggtgaa tatcctgttt aaaattaacg 
0701 gcaccaccga aattcagtaa GCAGAAAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTAT TACTCAACAG 
0771 GTAAGGCGCG AGGTTTTCCT TCAGGATCAA CCGCGACATA CTTAAATAAT