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PEC Original Annotations
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|
Essentiality
|
|
Class
|
non-essential
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|
References (PMID)
|
Protein Sci. 1992;1(12):1652-60.
Escherichia coli K12 arabinose-binding protein mutants with altered transport properties.
Kehres DG, Hogg RW.
(
1304895
)
|
|
|
Deletion
|
LD3-32-1
(LD)
,
OCL79
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
araF
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Related strains
|
|
Strains Search
|
Search strains by gene name:
araF
Search strains by all related name:
araF b1901 ECK1899 JW1889
|
General information (Go to Linear View: )
|
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Gene Name
|
araF
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|
Alternative name
|
b1901,ECK1899,JW1889
|
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Location, Length
|
1,983,163 - 1,984,152
(
-
)
;
42.74
min
;
990
(bp) ,
329
(aa)
|
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Product
|
L-arabinose transporter subunit
|
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Operon Name
|
araFGH
|
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Note
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L-arabinose-binding periplasmic protein; periplasmic-binding component of ABC superfamily; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0016052 - carbohydrate catabolic process
|
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Function
|
transport; Transport of small molecules: Carbohydrates, organic acids, alcohols
|
|
Gene Ontology
|
GO:0005351
;
sugar:hydrogen ion symporter activity ( araF )
GO:0006810
;
transport ( araF )
GO:0008643
;
carbohydrate transport ( araF )
GO:0042597
;
periplasmic space ( araF )
|
|
PID
|
1788211
|
EC number
(KEGG Pathway)
|
|
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
araF
(
EG10057
)
|
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Number of removed
|
23 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
ARAF_ECOLI
(
P02924
)
|
|
- Protein name
|
L-arabinose-binding periplasmic protein precursor
|
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- Synonyms
|
ABP
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- Gene name
|
Name=araF; OrderedLocusNames=b1901;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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araF
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain , L-Arabinose-Binding Protein Complex With D-Galactose
|
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SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
L-arabinose-binding periplasmic protein precursor
|
|
PSI-BLAST
|
6-phosphofructokinase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
L-arabinose transporter subunit [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
|
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MMBR References
|
J Bacteriol. 1981;148(2):472-9.
L-arabinose transport systems in Escherichia coli K-12.
Kolodrubetz D, Schleif R.
(
7028715
)
|
J Mol Biol. 1981;151(2):215-27.
Regulation of the L-arabinose transport operons in Escherichia coli.
Kolodrubetz D, Schleif R.
(
6461771
)
|
J Mol Biol. 1982;156(1):53-66.
Arabinose-inducible promoter from Escherichia coli. Its cloning from chromosomal DNA, identification as the araFG promoter and sequence.
Kosiba BE, Schleif R.
(
7047752
)
|
J Mol Biol. 1987;197(1):37-46.
High-affinity L-arabinose transport operon. Nucleotide sequence and analysis of gene products.
Scripture JB, Voelker C, Miller S, O'Donnell RT, Polgar L, Rade J, Horazdovsky BF, Hogg RW.
(
2445996
)
|
J Biol Chem. 1983;258:10853-10855
The nucleotide sequences defining the signal peptides of the galactose-binding protein and the arabinose-binding protein.
Scripture, J. B., and R. W. Hogg.
|
J Bacteriol. 1981;147(3):920-4.
High-affinity arabinose transport mutants of Escherichia coli: isolation and gene location.
Clark AF, Hogg RW.
(
7024251
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MHKFTKALAA IGLAAVMSQS AMAENLKLGF LVKQPEEPWF QTEWKFADKA GKDLGFEVIK IAVPDGEKTL
0071 NAIDSLAASG AKGFVICTPD PKLGSAIVAK ARGYDMKVIA VDDQFVNAKG KPMDTVPLVM MAATKIGERQ
0141 GQELYKEMQK RGWDVKESAV MAITANELDT ARRRTTGSMD ALKAAGFPEK QIYQVPTKSN DIPGAFDAAN
0211 SMLVQHPEVK HWLIVGMNDS TVLGGVRATE GQGFKAADII GIGINGVDAV SELSKAQATG FYGSLLPSPD
0281 VHGYKSSEML YNWVAKDVEP PKFTEVTDVV LITRDNFKEE LEKKGLGGK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttatctttta gttaaaaggt aatgctttgt
-070 tttccgatta atttaacgaa tgtcattcgt ttttgcccta cacaaaacga cactaaagct ggagagaacc
0001 atgcacaaat ttactaaagc cctggcagcc attggtctgg cagccgttat gtcacaatcc gctatggcgg
0071 agaacctgaa gctcggtttt ctggtgaagc aaccggaaga gccgtggttc cagaccgaat ggaagtttgc
0141 cgataaagcc gggaaggatt tagggtttga ggttattaag attgccgtgc cggatggcga aaaaacattg
0211 aacgcgatcg acagcctggc tgccagtggc gcaaaaggtt tcgttatttg tactccggac cccaaactcg
0281 gctctgccat cgtcgcgaaa gcgcgtggct acgatatgaa agtcattgcc gtggatgacc agtttgttaa
0351 cgccaaaggt aagccaatgg ataccgttcc gctggtgatg atggcggcga ctaaaattgg cgaacgtcag
0421 ggccaggaac tgtataaaga gatgcagaaa cgtggctggg atgtcaaaga aagcgcggtg atggcgatta
0491 ccgccaacga actggatacc gcccgccgcc gtactacggg atctatggat gcgctgaaag cggccggatt
0561 cccggaaaaa caaatttatc aggtacctac caaatctaac gacatcccgg gggcatttga cgctgccaac
0631 tcaatgctgg ttcaacatcc ggaagttaaa cattggctga tcgtcggtat gaacgacagc accgtgctgg
0701 gcggcgtacg cgcgacggaa ggtcagggct ttaaagcggc cgatatcatc ggcattggca ttaacggtgt
0771 ggatgcggtg agcgaactgt ctaaagcaca ggcaaccggc ttctacggtt ccctgctgcc aagcccggac
0841 gtacatggct ataaatccag cgaaatgctt tacaactggg tagcaaaaga cgttgaaccg ccaaaattta
0911 ccgaagttac cgacgtggta ctgatcacgc gtgacaactt taaagaagaa ctggagaaaa aaggtttagg
0981 cggtaagtaa tttgccggaa aaattcccct ctgcatgatg cagagggggt gtgaacgacc agtgattcac
1051 ggagacgtta tgcaacagtc taccccgtat ctctcatttc
|
