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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(bioH-asd)29
,
OCL18,19-8 (AK1-13 / BK2-13)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
glgB
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Related strains
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Strains Search
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Search strains by gene name:
glgB
Search strains by all related name:
glgB b3432 ECK3418 f728 JW3395
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General information (Go to Linear View: )
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Gene Name
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glgB
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Alternative name
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b3432,ECK3418,f728,JW3395
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Location, Length
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3,569,339 - 3,571,525
(
-
)
;
76.93
min
;
2187
(bp) ,
728
(aa)
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Product
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1,4-alpha-glucan branching enzyme
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Operon Name
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glgBXCAP
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0000271 - polysaccharide biosynthetic process
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Function
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enzyme; Macromolecule synthesis, modification: Polysaccharides - (cytoplasmic)
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Gene Ontology
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GO:0003824
;
catalytic activity ( glgB )
GO:0003844
;
1,4-alpha-glucan branching enzyme activity ( glgB )
GO:0004553
;
hydrolase activity, hydrolyzing O-glycosyl compounds ( glgB )
GO:0005975
;
carbohydrate metabolic process ( glgB )
GO:0005978
;
glycogen biosynthetic process ( glgB )
GO:0016740
;
transferase activity ( glgB )
GO:0016757
;
transferase activity, transferring glycosyl groups ( glgB )
GO:0043169
;
cation binding ( glgB )
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PID
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1789839
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EC number
(KEGG Pathway)
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2.4.1.18
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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GLGB_ECOLI
(
P07762
)
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- Protein name
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1,4-alpha-glucan branching enzyme
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- Synonyms
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EC 2.4.1.18, Glycogen branching enzyme, BE, 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl- transferase
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- Gene name
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Name=glgB; OrderedLocusNames=b3432;
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MMBR References
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J Biol Chem. 1986;261:8738-8743
Biosynthesis of bacterial glycogen: primary structure of Escherichia coli 1,4-a-D-glucan-6-a-D-(1,4-a-D-glucano)-transferase as deduced from the nucleotide sequence of the glgB gene.
Baecker, P. A., J. Greenberg, and J. Preiss.
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Mol Gen Genet. 1977;150(3):325-8.
Relative order of glg mutations affecting glycogen biosynthesis in Escherichia coli K12.
Latil-Damotte M, Lares C.
(
321940
)
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Gene. 1988;70(2):363-76.
Analysis of the Escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes.
Romeo T, Kumar A, Preiss J.
(
2975249
)
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Gene. 1991;108(1):23-9.
A simple method for cloning genes involved in glucan biosynthesis: isolation of structural and regulatory genes for glycogen synthesis in Escherichia coli.
Romeo T, Moore J, Smith J.
(
1662181
)
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J Bacteriol. 1980;144(1):438-40.
Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell envelope gene murG.
Salmond GP, Lutkenhaus JF, Donachie WD.
(
6998962
)
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| Sequences |
Amino acid
FASTA format
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0001 MSDRIDRDVI NALIAGHFAD PFSVLGMHKT TAGLEVRALL PDATDVWVIE PKTGRKLAKL ECLDSRGFFS
0071 GVIPRRKNFF RYQLAVVWHG QQNLIDDPYR FGPLIQEMDA WLLSEGTHLR PYETLGAHAD TMDGVTGTRF
0141 SVWAPNARRV SVVGQFNYWD GRRHPMRLRK ESGIWELFIP GAHNGQLYKY EMIDANGNLR LKSDPYAFEA
0211 QMRPETASLI CGLPEKVVQT EERKKANQFD APISIYEVHL GSWRRHTDNN FWLSYRELAD QLVPYAKWMG
0281 FTHLELLPIN EHPFDGSWGY QPTGLYAPTR RFGTRDDFRY FIDAAHAAGL NVILDWVPGH FPTDDFALAE
0351 FDGTNLYEHS DPREGYHQDW NTLIYNYGRR EVSNFLVGNA LYWIERFGID ALRVDAVASM IYRDYSRKEG
0421 EWIPNEFGGR ENLEAIEFLR NTNRILGEQV SGAVTMAEES TDFPGVSRPQ DMGGLGFWYK WNLGWMHDTL
0491 DYMKLDPVYR QYHHDKLTFG ILYNYTENFV LPLSHDEVVH GKKSILDRMP GDAWQKFANL RAYYGWMWAF
0561 PGKKLLFMGN EFAQGREWNH DASLDWHLLE GGDNWHHGVQ RLVRDLNLTY RHHKAMHELD FDPYGFEWLV
0631 VDDKERSVLI FVRRDKEGNE IIVASNFTPV PRHDYRFGIN QPGKWREILN TDSMHYHGSN AGNGGTVHSD
0701 EIASHGRQHS LSLTLPPLAT IWLVREAE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 ggatgcacag agtgctgcgc cgttcaggtc
-070 aaaaaaatgt cacaaccaga agtcaaaaat ccaattggat ggggtgacac aataaaacag gaagacaagc
0001 atgtccgatc gtatcgatag agacgtgatt aacgcgctaa ttgcaggcca ttttgcggat cctttttccg
0071 tactgggaat gcataaaacc accgcgggac tggaagtccg tgccctttta cccgacgcta ccgatgtgtg
0141 ggtgattgaa ccgaaaaccg ggcgcaaact cgcaaaactg gagtgtctcg actcacgggg attctttagc
0211 ggcgtcattc cgcgacgtaa gaattttttc cgctatcagt tggctgttgt ctggcatggt cagcaaaacc
0281 tgattgatga tccttaccgt tttggtccgc taatccagga aatggatgcc tggctattat ctgaaggtac
0351 tcacctgcgc ccgtatgaaa ccttaggcgc gcatgcagat actatggatg gcgtcacagg tacgcgtttc
0421 tctgtctggg ctccaaacgc ccgtcgggtc tcggtggttg ggcaattcaa ctactgggac ggtcgccgtc
0491 acccgatgcg cctgcgtaaa gagagcggca tctgggaact gtttatccct ggggcgcata acggtcagct
0561 ctataaatac gagatgattg atgccaatgg caacttgcgt ctgaagtccg acccttatgc ctttgaagcg
0631 caaatgcgcc cggaaaccgc gtctcttatt tgcgggctgc cggaaaaggt tgtacagact gaagagcgca
0701 aaaaagcgaa tcagtttgat gcgccaatct ctatttatga agttcacctg ggttcctggc gtcgccacac
0771 cgacaacaat ttctggttga gctaccgcga gctggccgat caactggtgc cttatgctaa atggatgggc
0841 tttacccacc tcgaactact gcccattaac gagcatccct tcgatggcag ttggggttat cagccaaccg
0911 gcctgtatgc gccaacccgc cgttttggta ctcgcgacga cttccgttat ttcattgatg ccgcacacgc
0981 agctggtctg aacgtgattc tcgactgggt gccaggccac ttcccgactg atgactttgc gcttgccgaa
1051 tttgatggca cgaacttgta tgaacacagc gatccgcgtg aaggctatca tcaggactgg aacacgctga
1121 tctacaacta tggtcgccgt gaagtcagta acttcctcgt cggtaacgcg ctttactgga ttgaacgttt
1191 tggtattgat gcgctgcgcg tcgatgcggt ggcgtcaatg atttatcgcg actacagccg taaagagggg
1261 gagtggatcc cgaacgaatt tggcgggcgc gagaatcttg aagcgattga attcttgcgt aataccaacc
1331 gtattcttgg tgagcaggtt tccggtgcgg tgacaatggc tgaggagtct accgatttcc ctggcgtttc
1401 tcgtccgcag gatatgggcg gtctgggctt ctggtacaag tggaacctcg gctggatgca tgacaccctg
1471 gactacatga agctcgaccc ggtttatcgt cagtatcatc acgataaact gaccttcggg attctctaca
1541 actacactga aaacttcgtc ctgccgttgt cgcatgatga agtggtccac ggtaaaaaat cgattctcga
1611 ccgcatgccg ggcgacgcat ggcagaaatt cgcgaacctg cgcgcctact atggctggat gtgggcattc
1681 ccgggcaaga aactactgtt catgggtaac gaatttgccc agggccgcga gtggaaccat gacgccagcc
1751 tcgactggca tctgttggaa ggcggcgata actggcacca cggtgtccag cgtctggtgc gcgatctgaa
1821 cctcacctac cgccaccata aagcaatgca tgaactggat tttgacccgt acggctttga atggctggtg
1891 gtggatgaca aagaacgctc ggtgctgatc tttgtgcgtc gcgataaaga gggtaacgaa atcatcgttg
1961 ccagtaactt tacgccggta ccgcgtcatg attatcgctt cggcataaac cagccgggca aatggcgtga
2031 aatcctcaat accgattcca tgcactatca cggcagtaat gcaggcaatg gcggcacggt acacagcgat
2101 gagattgcca gccacggtcg tcagcattca ctaagcctga cgctaccacc gctggccact atctggctgg
2171 ttcgggaggc agaatgacac aactcgccat tggcaaaccc gctcccctcg gcgcgcatta cgacggtcag
2241 ggcgtcaact tcacactttt ctccgctcat gccgagcggg tagaact
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