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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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|
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Deletion
|
22
(LD)
,
OCL49,51-3 (LD 22B_CRS)
(D)
,
OCL49,51-4 (LD 22B_P/O)
(D)
,
OCL49-1
(D)
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|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
proV
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Related strains
|
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Strains Search
|
Search strains by gene name:
proV
Search strains by all related name:
proV b2677 ECK2671 JW2652 o400 osrA proU
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General information (Go to Linear View: )
|
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Gene Name
|
proV
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Alternative name
|
b2677,ECK2671,JW2652,o400,osrA,proU
|
|
Location, Length
|
2,802,837 - 2,804,039
(
+
)
;
60.41
min
;
1203
(bp) ,
400
(aa)
|
|
Product
|
glycine betaine transporter subunit
|
|
Operon Name
|
proVWX
|
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Note
|
ATP-binding component of transport system for glycine, betaine and proline; GO_component: GO:0005737 - cytoplasm;GO_function: GO:0015596 - glycine betaine/proline porter activity; GO_process: GO:0006561 - proline biosynthetic process
|
|
Function
|
transport; Transport of small molecules: Amino acids, amines
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( proV )
GO:0005515
;
protein binding ( proV )
GO:0005524
;
ATP binding ( proV )
GO:0005886
;
plasma membrane ( proV )
GO:0006810
;
transport ( proV )
GO:0006865
;
amino acid transport ( proV )
GO:0015171
;
amino acid transmembrane transporter activity ( proV )
GO:0016020
;
membrane ( proV )
GO:0016887
;
ATPase activity ( proV )
GO:0017111
;
nucleoside-triphosphatase activity ( proV )
|
|
PID
|
1789032
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
PROV_ECOLI
(
P14175
)
|
|
- Protein name
|
Glycine betaine/L-proline transport ATP-binding protein proV.
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- Synonyms
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- Gene name
|
Name=proV; OrderedLocusNames=b2677;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
proV
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain 1, Malk
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Glycine betaine/L-proline transport ATP-binding protein proV
|
|
PSI-BLAST
|
Methionine import ATP-binding protein metN
|
|
nr
(database updated :
2007.02.20
)
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BLAST
|
glycine betaine transporter subunit [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
|
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PROSITE
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GLYCOSAMINOGLYCAN
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
ATP_GTP_A
ABC_TRANSPORTER
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MMBR References
|
Mol Microbiol. 1988;2(2):265-79.
Cloned structural genes for the osmotically regulated binding-protein-dependent glycine betaine transport system (ProU) of Escherichia coli K-12.
Faatz E, Middendorf A, Bremer E.
(
2837616
)
|
Microbiol. Rev. 1985;49:379-397.
Structure and organization of the transfer ribonucleic acid genes of Escherichia coli K-12.
Fournier, M. J., and H. Ozeki.
|
J Bacteriol. 1989;171(4):1923-31.
Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.
Gowrishankar J.
(
2649479
)
|
J Bacteriol. 1986;168(3):1197-204.
Molecular cloning of an osmoregulatory locus in Escherichia coli: increased proU gene dosage results in enhanced osmotolerance.
Gowrishankar J, Jayashree P, Rajkumari K.
(
3536868
)
|
J Bacteriol. 1986;166(1):253-9.
Proline transport and osmotic stress response in Escherichia coli K-12.
Grothe S, Krogsrud RL, McClellan DJ, Milner JL, Wood JM.
(
3514577
)
|
Mol Microbiol. 1989;3(11):1521-31.
Characterization of the osmoregulated Escherichia coli proU promoter and identification of ProV as a membrane-associated protein.
May G, Faatz E, Lucht JM, Haardt M, Bolliger M, Bremer E.
(
2515417
)
|
Mol Microbiol. 1989;3(8):1025-38.
Molecular characterization of the proU loci of Salmonella typhimurium and Escherichia coli encoding osmoregulated glycine betaine transport systems.
Stirling DA, Hulton CS, Waddell L, Park SF, Stewart GS, Booth IR, Higgins CF.
(
2691838
)
|
J Bacteriol. 1986;168(2):805-14.
Osmotic regulation of transcription: induction of the proU betaine transport gene is dependent on accumulation of intracellular potassium.
Sutherland L, Cairney J, Elmore MJ, Booth IR, Higgins CF.
(
3536861
)
|
J Bacteriol. 1989;171(4):1915-22.
Osmoregulation in Escherichia coli: complementation analysis and gene-protein relationships in the proU locus.
Dattananda CS, Gowrishankar J.
(
2649478
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MAIKLEIKNL YKIFGEHPQR AFKYIEQGLS KEQILEKTGL SLGVKDASLA IEEGEIFVIM GLSGSGKSTM
0071 VRLLNRLIEP TRGQVLIDGV DIAKISDAEL REVRRKKIAM VFQSFALMPH MTVLDNTAFG MELAGINAEE
0141 RREKALDALR QVGLENYAHS YPDELSGGMR QRVGLARALA INPDILLMDE AFSALDPLIR TEMQDELVKL
0211 QAKHQRTIVF ISHDLDEAMR IGDRIAIMQN GEVVQVGTPD EILNNPANDY VRTFFRGVDI SQVFSAKDIA
0281 RRTPNGLIRK TPGFGPRSAL KLLQDEDREY GYVIERGNKF VGAVSIDSLK TALTQQQGLD AALIDAPLAV
0351 DAQTPLSELL SHVGQAPCAV PVVDEDQQYV GIISKGMLLR ALDREGVNNG
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AGGGGTTGCC TCAGATTCTC AGTATGTTAG
-070 GGTAGAAAAA AGTGACTATT TCCATTGGGT AATATATCGA CATAGACAAA TAAAGGAATC TTTCTATTGC
0001 atggcaatta aattagaaat taaaaatctt tataaaatat ttggcgagca tccacagcga gcgttcaaat
0071 atatcgaaca aggactttca aaagaacaaa ttctggaaaa aactgggcta tcgcttggcg taaaagacgc
0141 cagtctggcc attgaagaag gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg
0211 gtacgccttc tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
0281 aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt cctttgcctt
0351 aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg ccggaattaa tgccgaagaa
0421 cgccgggaaa aagcccttga tgcactgcgt caggtcgggc tggaaaatta tgcccacagc tacccggatg
0491 aactctctgg cgggatgcgt caacgtgtgg gattagcccg cgcgttagcg attaatccgg atatattatt
0561 aatggacgaa gccttctcgg cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta
0631 caggcgaaac atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc
0701 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca ataatccggc
0771 gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtat tcagtgcgaa agatattgcc
0841 cgccggacac cgaatggctt aattcgtaaa acccctggct tcggcccacg ttcggcactg aaattattgc
0911 aggatgaaga tcgcgaatat ggctacgtta tcgaacgcgg taataagttt gtcggcgcag tctccatcga
0981 ttcgcttaaa accgcgttaa cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc
1051 gatgcacaaa cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg
1121 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc gtgagggggt
1191 aaataatggc tgaTCAAAAT AATCCGTGGG ATACCACGCC AGCGGCGGAC AGTGCCGCGC AATCCGCAGA
1261 CGCCTGGGGT ACACCGACGA CTGCACCGAC TGACGGCGGT GGT
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