MG1655
W3110
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Gene Report : leuO
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR30 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  leuO
Related strains
     Strains Search Search strains by gene name:  leuO   Search strains by all related name:  leuO b0076 ECK0078 JW0075

General information  (Go to Linear View:)
 Gene Name leuO  
 Alternative name b0076,ECK0078,JW0075  
 Location, Length 84,368 - 85,312 (  +  ) ;   1.82 min ; 945 (bp) ,   314 (aa) Go to Linear View
 Product DNA-binding transcriptional activator  
 Operon Name leuO  
 Note probable transcriptional activator for leuABCD operon; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_process: GO:0009098 - leucine biosynthetic process; GO_process: GO:0006350 - transcription  
 Function putative regulator; Amino acid biosynthesis: Leucine  
 Gene Ontology GO:0003677; DNA binding ( leuO )
GO:0003700; transcription factor activity ( leuO )
GO:0006350; transcription ( leuO )
GO:0006355; regulation of transcription, DNA-dependent ( leuO )
 PID 87081684  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) LEUO_ECOLI ( P10151 ; P75640 )
    -  Protein name Probable HTH-type transcriptional regulator leuO.  
    -  Synonyms  
    -  Gene name Name=leuO; OrderedLocusNames=b0076;  

 Linear View (Whole Mode)
View Location
   75.0  –  100.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP leuO (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Dntr From Burkholderia Sp. Strain Dnt In Complex With Thiocyanate  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Probable HTH-type transcriptional regulator leuO  
         PSI-BLAST Probable HTH-type transcriptional regulator leuO
    nr     (database updated : 2007.02.20 )
         BLAST COG0583: Transcriptional regulator [Escherichia coli 101-1]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    HTH_LYSR_FAMILY    AA_TRNA_LIGASE_II_2     

Other Cross-Reference
    COG COG0583K 
    EcoCyc leuO 

MMBR References
Mol Microbiol. 1997;25(3):559-69.
The LysR-like regulator LeuO in Escherichia coli is involved in the translational regulation of rpoS by affecting the expression of the small regulatory DsrA-RNA.
Klauck E, Bohringer J, Hengge-Aronis R. ( 9302018 )
J Bacteriol. 1995;177:810-814
Effects of multicopy LeuO on the expression of the acid-inducible lysine decarboxylase gene in Escherichia coli.
Shi, X., and G. N. Bennett.
J Bacteriol. 1998;180:190-193
The leuO gene product has a latent ability to relieve bgl silencing in Escherichia coli.
Ueguchi, C., T. Ohta, C. Seto, T. Suzuki, and T. Mizuno.

Sequences
Amino acid
FASTA format
0001 MPEVQTDHPE TAELSKPQLR MVDLNLLTVF DAVMQEQNIT RAAHVLGMSQ PAVSNAVARL KVMFNDELFV 
0071 RYGRGIQPTA RAFQLFGSVR QALQLVQNEL PGSGFEPASS ERVFHLCVCS PLDSILTSQI YNHIEQIAPN 
0141 IHVMFKSSLN QNTEHQLRYQ ETEFVISYED FHRPEFTSVP LFKDEMVLVA SKNHPTIKGP LLKHDVYNEQ 
0211 HAAVSLDRFA SFSQPWYDTV DKQASIAYQG MAMMSVLSVV SQTHLVAIAP RWLAEEFAES LELQVLPLPL 
0281 KQNSRTCYLS WHEAAGRDKG HQWMEEQLVS ICKR

Nucleotide
FASTA format

View sequence in neighbor 100bp
0001 atgccagagg tacaaacaga tcatccagag acggcggagt taagcaaacc acagctacgc atggtcgatc 
0071 tcaacttatt aaccgttttc gatgccgtga tgcaggagca aaacattact cgtgccgctc atgttctggg 
0141 aatgtcgcaa cctgcggtca gtaacgctgt tgcacgcctg aaggtgatgt ttaatgacga gctttttgtt 
0211 cgttatggcc gtggtattca accgactgct cgcgcatttc aactttttgg ttcagttcgt caggcattgc 
0281 aactagtaca aaatgaattg cctggttcag gttttgaacc cgcgagcagt gaacgtgtat ttcatctttg 
0351 tgtttgcagc ccgttagaca gcattctgac ctcgcagatt tataatcaca ttgagcagat tgcgccaaat 
0421 atacatgtta tgttcaagtc ttcattaaat cagaacactg aacatcagct gcgttatcag gaaacggagt 
0491 ttgtgattag ttatgaagac ttccatcgtc ctgaatttac cagcgtacca ttatttaaag atgaaatggt 
0561 gctggtagcc agcaaaaatc atccaacaat taagggcccg ttactgaaac atgatgttta taacgaacaa 
0631 catgcggcgg tttcgctcga tcgtttcgcg tcatttagtc aaccttggta tgacacggta gataagcaag 
0701 ccagtatcgc gtatcagggc atggcaatga tgagcgtact tagcgtggtg tcgcaaacgc atttggtcgc 
0771 tattgcgccg cgttggctgg ctgaagagtt cgctgaatcc ttagaattac aggtattacc gctgccgtta 
0841 aaacaaaaca gcagaacctg ttatctctcc tggcatgaag ctgccgggcg cgataaaggc catcagtgga 
0911 tggaagagca attagtctca atttgcaaac gctaa