MG1655
W3110
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Gene Report : argF
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Microbiol Mol Biol Rev. 1998;62(3):814-984
Linkage map of Escherichia coli K-12, edition 10: the traditional map.
Berlyn MK. ( 9729611 )
     Deletion 9-1 (LD)  ,  DE(gpt-lac)5  ,  OCR36,37-6 (LD 9C::Ap) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  argF
Related strains
     Strains Search Search strains by gene name:  argF   Search strains by all related name:  argF Arg5 argD b0273 ECK0274 JW0266

General information  (Go to Linear View:)
 Gene Name argF  
 Alternative name Arg5,argD,b0273,ECK0274,JW0266  
 Location, Length 288,525 - 289,529 (  -  ) ;   6.22 min ; 1005 (bp) ,   334 (aa) Go to Linear View
 Product ornithine carbamoyltransferase 2, chain F; CP4-6 prophage  
 Operon Name argF  
 Note ornithine carbamoyltransferase 2, chain F; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006526 - arginine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Arginine; Phage or Prophage Related  
 Gene Ontology GO:0003824; catalytic activity ( argD )
GO:0003992; acetylornithine transaminase activity ( argD )
GO:0004585; ornithine carbamoyltransferase activity ( argF )
GO:0005737; cytoplasm ( argD )
GO:0005737; cytoplasm ( argF )
GO:0006520; amino acid metabolic process ( argF )
GO:0006525; arginine metabolic process ( argD )
GO:0006526; arginine biosynthetic process ( argD )
GO:0006526; arginine biosynthetic process ( argF )
GO:0008483; transaminase activity ( argD )
GO:0008652; amino acid biosynthetic process ( argD )
GO:0008652; amino acid biosynthetic process ( argF )
GO:0009016; succinyldiaminopimelate transaminase activity ( argD )
GO:0009085; lysine biosynthetic process ( argD )
GO:0009348; ornithine carbamoyltransferase complex ( argF )
GO:0016597; amino acid binding ( argF )
GO:0016740; transferase activity ( argD )
GO:0016740; transferase activity ( argF )
GO:0016743; carboxyl- or carbamoyltransferase activity ( argF )
GO:0030170; pyridoxal phosphate binding ( argD )
 PID 1786469  
 EC number
  (KEGG Pathway)
 
2.1.3.3  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene argF ( EG10067 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 3 more] )
  botton Entry name(Acc.no) OTC2_ECOLI ( P06960 ; P78308 )
    -  Protein name Ornithine carbamoyltransferase chain F  
    -  Synonyms EC 2.1.3.3, OTCase-2  
    -  Gene name Name=argF; OrderedLocusNames=b0273;  

 Linear View (Whole Mode)
View Location
   280.0  –  305.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP argF (  homologous genes of other bacterias  )   argD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Ornithine Transcarbamylase From Escherichia Coli  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Ornithine carbamoyltransferase chain F  
         PSI-BLAST Ornithine carbamoyltransferase
    nr     (database updated : 2007.02.20 )
         BLAST CP4-6 prophage; ornithine carbamoyltransferase 2, chain F [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    CARBAMOYLTRANSFERASE     

Other Cross-Reference
    COG COG0078E 
    EcoCyc argF 

MMBR References
Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC. ( 6307829 )
J Bacteriol. 1992;174(11):3836-7.
Physical map location of the argFGH operon of Escherichia coli.
Hendrickson W, Rudd KE. ( 1592837 )
Mol Gen Genet. 1981;181(2):222-9.
Mapping of IS1 elements flanking the argF gene region on the Escherichia coli K-12 chromosome.
Hu M, Deonier RC. ( 6268939 )
Mol Gen Genet. 1985;201(2):347-50.
Amplification of the ArgF region in strain HfrP4X of E. coli K-12.
Jessop AP, Clugston C. ( 3003538 )
Eur J Biochem. 1972;27(1):93-102.
The dual genetic control of ornithine carbamolytransferase in Escherichia coli. A case of bacterial hybrid enzymes.
Legrain C, Halleux P, Stalon V, Glansdorff N. ( 4558857 )
Gene. 1979;5(2):159-75.
Mapping of restriction sites in the argF gene of Escherichia coli by partial endonuclease digestion of end-labeled DNA.
Moore SK, James E. ( 376404 )
Gene. 1981;16(1-3):119-32.
Nucleotide sequence of the argF regulatory region of Escherichia coli K-12.
Moore SK, Garvin RT, James E. ( 6282686 )
EMBO J. 1982;1(7):853-7.
Homologous control sites and DNA transcription starts in the related argF and argI genes of Escherichia coli K12.
Piette J, Cunin R, Van Vliet F, Charlier D, Crabeel M, Ota Y, Glansdorff N. ( 6329710 )
Nucleic Acids Res. 1984;12:6277-6289
Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferase-complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB.
Van Vliet, F., R. Cunin, A. Jacobs, J. Piette, D. Gigot, M. Lauwereys, A. Pierard, and N. Glansdorff.
Mol Gen Genet. 1981;181(2):230-40.
Characterization of P1argF derivatives from Escherichia coli K12 transduction. I. IS1 elements flank the argF gene segment.
York MK, Stodolsky M. ( 6268940 )
Gene. 1979;5(3):207-31.
Cloning and endonuclease restriction analysis of argF and of the control region of the argECBH bipolar operon in Escherichia coli.
Crabeel M, Charlier D, Cunin R, Glansdorff N. ( 381103 )

Sequences
Amino acid
FASTA format
0001 MSDLYKKHFL KLLDFTPAQF TSLLTLAAQL KADKKNGKEV QKLTGKNIAL IFEKDSTRTR CSFEVAAFDQ 
0071 GARVTYLGPS GSQIGHKESI KDTARVLGRM YDGIQYRGHG QEVVETLAQY AGVPVWNGLT NEFHPTQLLA 
0141 DLMTMQEHLP GKAFNEMTLV YAGDARNNMG NSMLEAAALT GLDLRLLAPK ACWPEESLVA ECSALAEKHG 
0211 GKITLTEDVA AGVKGADFIY TDVWVSMGEA KEKWAERIAL LRGYQVNAQM MALTDNPNVK FLHCLPAFHD 
0281 DQTTLGKQMA KEFDLHGGME VTDEVFESAA SIVFDQAENR MHTIKAVMMA TLGE

Nucleotide
FASTA format

View sequence in neighbor 100bp
0001 atgtccgatt tatacaaaaa acactttctg aaactgctcg actttacccc tgcacagttc acttctctgc 
0071 tgacccttgc cgcacagctc aaagccgata aaaaaaatgg caaggaagta cagaagctta ccggtaaaaa 
0141 catcgcgctc atcttcgaaa aagactcgac tcgtacccgt tgctctttcg aagttgccgc atttgaccag 
0211 ggcgcgcgcg ttacctattt agggccgagc ggcagccaga ttgggcataa agagtcaatt aaggacaccg 
0281 cgcgggttct cgggcggatg tatgacggca ttcagtatcg cggtcacggc caggaagtgg tcgaaacgct 
0351 ggcgcagtat gcgggcgtgc cggtgtggaa cgggctgacc aacgagttcc acccgaccca gctgctggcg 
0421 gacctgatga ccatgcagga gcacctgccg ggcaaggcgt ttaacgagat gacgctggtc tacgcgggcg 
0491 atgcgcgcaa caacatgggc aactcgatgc tggaagcggc ggcgctgacc gggctggatc tgcgcctgtt 
0561 ggccccgaaa gcctgctggc cggaagagag cctggtggcg gagtgcagcg cgctggcgga gaagcacggc 
0631 gggaaaatta ctctgacgga agacgtggcg gcaggcgtta agggcgcgga ctttatctat accgacgtgt 
0701 gggtgtcgat gggcgaggcc aaagagaagt gggcagagcg gattgcgctg ctgcgcgggt atcaggtgaa 
0771 cgcgcagatg atggcgctga ccgacaaccc gaacgtgaag ttcctgcact gtctgccggc gttccatgac 
0841 gaccagacta cgctcggcaa gcagatggcg aaggagttcg atctgcacgg cgggatggag gtgacggacg 
0911 aggtgtttga gtcggcggcg agcatcgtgt tcgaccaggc ggaaaaccgg atgcatacga ttaaggcggt 
0981 gatgatggca acgcttgggg agtga