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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS.
(
9166795
)
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Deletion
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OCR30,31-3 (BK2-1 / AK1-1)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
murC
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Related strains
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Strains Search
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Search strains by gene name:
murC
Search strains by all related name:
murC b0091 ECK0092 JW0089
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General information (Go to Linear View: )
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Gene Name
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murC
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Alternative name
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b0091,ECK0092,JW0089
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Location, Length
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100,765 - 102,240
(
+
)
;
2.17
min
;
1476
(bp) ,
491
(aa)
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Product
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UDP-N-acetylmuramate:L-alanine ligase
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Operon Name
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mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC
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Note
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GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_process: GO:0009252 - peptidoglycan biosynthetic process
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Function
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enzyme; Murein sacculus, peptidoglycan
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Gene Ontology
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GO:0000166
;
nucleotide binding ( murC )
GO:0003824
;
catalytic activity ( murC )
GO:0005488
;
binding ( murC )
GO:0005524
;
ATP binding ( murC )
GO:0005737
;
cytoplasm ( murC )
GO:0007047
;
cell wall organization and biogenesis ( murC )
GO:0007049
;
cell cycle ( murC )
GO:0008152
;
metabolic process ( murC )
GO:0008360
;
regulation of cell shape ( murC )
GO:0008763
;
UDP-N-acetylmuramate-L-alanine ligase activity ( murC )
GO:0009058
;
biosynthetic process ( murC )
GO:0009252
;
peptidoglycan biosynthetic process ( murC )
GO:0009273
;
peptidoglycan-based cell wall biogenesis ( murC )
GO:0016874
;
ligase activity ( murC )
GO:0051301
;
cell division ( murC )
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PID
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1786279
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EC number
(KEGG Pathway)
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6.3.2.8
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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murC
(
EG10619
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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MURC_ECOLI
(
P17952
;
O07099
)
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- Protein name
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UDP-N-acetylmuramate--L-alanine ligase
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- Synonyms
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EC 6.3.2.8, UDP-N- acetylmuramoyl-L-alanine synthetase
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- Gene name
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Name=murC; OrderedLocusNames=b0091;
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- Comments
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FUNCTION:Cell wall formation.
CATALYTIC ACTIVITY:ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine.
PATHWAY:Peptidoglycan biosynthesis.
SUBCELLULAR LOCATION:Cytoplasmic (Probable).
SIMILARITY:Belongs to the murCDEF family.
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Entry name(Acc.no)
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MURC_ECOL6
(
Q8X9Y7
)
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- Protein name
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UDP-N-acetylmuramate--L-alanine ligase
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- Synonyms
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EC 6.3.2.8, UDP-N- acetylmuramoyl-L-alanine synthetase
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- Gene name
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Name=murC; OrderedLocusNames=c0109, z0101, ECs0095;
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- Comments
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FUNCTION:Cell wall formation.
CATALYTIC ACTIVITY:ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine.
PATHWAY:Peptidoglycan biosynthesis.
SUBCELLULAR LOCATION:Cytoplasmic (Probable).
SIMILARITY:Belongs to the murCDEF family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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murC
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Murc - Crystal Structure Of The Apo-Enzyme From Haemophilus Influenzae
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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UDP-N-acetylmuramate--L-alanine ligase
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PSI-BLAST
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UDP-N-acetylmuramate--L-alanine ligase
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nr
(database updated :
2007.02.20
)
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BLAST
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UDP-N-acetylmuramate--L-alanine ligase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
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MMBR References
|
Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS.
(
9166795
)
|
Nucleic Acids Res. 1990;18(13):4014
Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli.
Ikeda M, Wachi M, Jung HK, Ishino F, Matsuhashi M.
(
2197603
)
|
Biochemistry. 1996;35(5):1423-31.
Structural studies of Escherichia coli UDP-N-acetylmuramate:L-alanine ligase.
Jin H, Emanuele JJ Jr, Fairman R, Robertson JG, Hail ME, Ho HT, Falk PJ, Villafranca JJ.
(
8634272
)
|
J Bacteriol. 1989;171(11):6379-82.
Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes.
Jung HK, Ishino F, Matsuhashi M.
(
2553679
)
|
J Bacteriol. 1980;142(2):615-20.
Organization of genes in the ftsA-envA region of the Escherichia coli genetic map and identification of a new fts locus (ftsZ).
Lutkenhaus JF, Wolf-Watz H, Donachie WD.
(
6991482
)
|
J Bacteriol. 1980;143(3):1281-8.
Determination of transcriptional units and gene products from the ftsA region of Escherichia coli.
Lutkenhaus JF, Wu HC.
(
6447690
)
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| Sequences |
Amino acid
FASTA format
|
0001 MNTQQLAKLR SIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD LAPNPVTQQL MNLGATIYFN
0071 HRPENVRDAS VVVVSSAISA DNPEIVAAHE ARIPVIRRAE MLAELMRFRH GIAIAGTHGK TTTTAMVSSI
0141 YAEAGLDPTF VNGGLVKAAG VHARLGHGRY LIAEADESDA SFLHLQPMVA IVTNIEADHM DTYQGDFENL
0211 KQTFINFLHN LPFYGRAVMC VDDPVIRELL PRVGRQTTTY GFSEDADVRV EDYQQIGPQG HFTLLRQDKE
0281 PMRVTLNAPG RHNALNAAAA VAVATEEGID DEAILRALES FQGTGRRFDF LGEFPLEPVN GKSGTAMLVD
0351 DYGHHPTEVD ATIKAARAGW PDKNLVMLFQ PHRFTRTRDL YDDFANVLTQ VDTLLMLEVY PAGEAPIPGA
0421 DSRSLCRTIR GRGKIDPILV PDPARVAEML APVLTGNDLI LVQGAGNIGK IARSLAEIKL KPQTPEEEQH
0491 D
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CCACCGAGCG AGTGGCAAAT GAAGTGAGCC
-070 GGGTTGCCCG GGCGTAATTG TAGCGATGCC TTTTGCATCG TATGAATTTA AGAAGTTAAT GGCGTAAAGA
0001 atgaatacac aacaattggc aaaactgcgt tccatcgtgc ccgaaatgcg tcgcgttcgg cacatacatt
0071 ttgtcggcat tggtggtgcc ggtatgggcg gtattgccga agttctggcc aatgaaggtt atcagatcag
0141 tggttccgat ttagcgccaa atccggtcac gcagcagtta atgaatctgg gtgcgacgat ttatttcaac
0211 catcgcccgg aaaacgtacg tgatgccagc gtggtcgttg tttccagcgc gatttctgcc gataacccgg
0281 aaattgtcgc cgctcatgaa gcgcgtattc cggtgatccg tcgtgccgaa atgctggctg agttaatgcg
0351 ttttcgtcat ggcatcgcca ttgccggaac gcacggcaaa acgacaacca ccgcgatggt ttccagcatc
0421 tacgcagaag cggggctcga cccaaccttc gttaacggcg ggctggtaaa agcggcgggg gttcatgcgc
0491 gtttggggca tggtcggtac ctgattgccg aagcagatga gagtgatgca tcgttcctgc atctgcaacc
0561 gatggtggcg attgtcacca atatcgaagc cgaccacatg gatacctacc agggcgactt tgagaattta
0631 aaacagactt ttattaattt tctgcacaac ctgccgtttt acggtcgtgc ggtgatgtgt gttgatgatc
0701 cggtgatccg cgaattgtta ccgcgagtgg ggcgtcagac cacgacttac ggcttcagcg aagatgccga
0771 cgtgcgtgta gaagattatc agcagattgg cccgcagggg cactttacgc tgctgcgcca ggacaaagag
0841 ccgatgcgcg tcaccctgaa tgcgccaggt cgtcataacg cgctgaacgc cgcagctgcg gttgcggttg
0911 ctacggaaga gggcattgac gacgaggcta ttttgcgggc gcttgaaagc ttccagggga ctggtcgccg
0981 ttttgatttc ctcggtgaat tcccgctgga gccagtgaat ggtaaaagcg gtacggcaat gctggtcgat
1051 gactacggcc accacccgac ggaagtggac gccaccatta aagcggcgcg cgcaggctgg ccggataaaa
1121 acctggtaat gctgtttcag ccgcaccgtt ttacccgtac gcgcgacctg tatgatgatt tcgccaatgt
1191 gctgacgcag gttgataccc tgttgatgct ggaagtgtat ccggctggcg aagcgccaat tccgggagcg
1261 gacagccgtt cgctgtgtcg cacaattcgt ggacgtggga aaattgatcc cattctggtg ccggatccgg
1331 cgcgggtagc cgagatgctg gcaccggtat taaccggtaa cgacctgatt ctcgttcagg gggctggtaa
1401 tattggaaaa attgcccgtt ctttagctga aatcaaactg aagccgcaaa ctccggagga agaacaacat
1471 gactgaTAAA ATCGCGGTCC TGTTGGGTGG GACCTCCGCT GAGCGGGAAG TTTCTCTGAA TTCTGGCGCA
1541 GCGGTGTTAG CCGGACTGCG TGAAGGCGGT ATTGAC
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