MG1655
W3110
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Gene Report : murG
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1991;173(15):4625-36.
The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis.
Mengin-Lecreulx D, Texier L, Rousseau M, van Heijenoort J. ( 1649817 )
     Deletion OCR30,31-3 (BK2-1 / AK1-1) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  murG
Related strains
     Strains Search Search strains by gene name:  murG   Search strains by all related name:  murG b0090 ECK0091 JW0088

General information  (Go to Linear View:)
 Gene Name murG  
 Alternative name b0090,ECK0091,JW0088  
 Location, Length 99,644 - 100,711 (  +  ) ;   2.15 min ; 1068 (bp) ,   355 (aa) Go to Linear View
 Product N-acetylglucosaminyl transferase  
 Operon Name mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009252 - peptidoglycan biosynthetic process  
 Function enzyme; Murein sacculus, peptidoglycan,1.6.7 metabolism; macromolecules (cellular constituent) biosynthesis; peptidoglycan (murein),7.3 location of gene products; inner membrane  
 Gene Ontology GO:0005886 ; plasma membrane ( murG )
GO:0005975 ; carbohydrate metabolic process ( murG )
GO:0007047 ; cell wall organization and biogenesis ( murG )
GO:0007049 ; cell cycle ( murG )
GO:0008360 ; regulation of cell shape ( murG )
GO:0009252 ; peptidoglycan biosynthetic process ( murG )
GO:0009276 ; 1-2nm peptidoglycan-based cell wall ( murG )
GO:0016020 ; membrane ( murG )
GO:0016740 ; transferase activity ( murG )
GO:0016757 ; transferase activity, transferring glycosyl groups ( murG )
GO:0016758 ; transferase activity, transferring hexosyl groups ( murG )
GO:0019277 ; UDP-N-acetylgalactosamine biosynthetic process ( murG )
GO:0030246 ; carbohydrate binding ( murG )
GO:0030259 ; lipid glycosylation ( murG )
GO:0050511 ; undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity ( murG )
GO:0051301 ; cell division ( murG )
 PID 1786278  
 EC number
  (KEGG Pathway)   		2.4.1.227  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene murG ( EG10623 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) MURG_ECOLI ( P17443 )
    -  Protein name UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase  
    -  Synonyms EC 2.4.1.227, Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase  
    -  Gene name Name=murG; OrderedLocusNames=b0090;  
    -  Comments
  • FUNCTION:Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).
  • CATALYTIC ACTIVITY:UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol.
  • PATHWAY:Peptidoglycan biosynthesis; last step.
  • SUBCELLULAR LOCATION:Inner membrane-associated.
  • SIMILARITY:Belongs to the glycosyltransferase family 28. MurG subfamily.  
    •   botton Entry name(Acc.no) MURG_ECOL6 ( Q8FL64 )
        -  Protein name UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase  
        -  Synonyms EC 2.4.1.227, Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase  
        -  Gene name Name=murG; OrderedLocusNames=c0108;  
        -  Comments
  • FUNCTION:Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) (By similarity).
  • CATALYTIC ACTIVITY:UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol.
  • PATHWAY:Peptidoglycan biosynthesis; last step.
  • SUBCELLULAR LOCATION:Inner membrane-associated (By similarity).
  • SIMILARITY:Belongs to the glycosyltransferase family 28. MurG subfamily.  
    •   botton Entry name(Acc.no) MURG_ECO57 ( Q8X9Y8 )
        -  Protein name UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase  
        -  Synonyms EC 2.4.1.227, Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase  
        -  Gene name Name=murG; OrderedLocusNames=z0100, ECs0094;  
        -  Comments
  • FUNCTION:Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) (By similarity).
  • CATALYTIC ACTIVITY:UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol.
  • PATHWAY:Peptidoglycan biosynthesis; last step.
  • SUBCELLULAR LOCATION:Inner membrane-associated (By similarity).
  • SIMILARITY:Belongs to the glycosyltransferase family 28. MurG subfamily.  

    •  Linear View (Whole Mode)
    View Location
       90.0  –  115.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP murG (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, The 1.9 Angstrom Crystal Structure Of E. Coli Murg  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST UDP-N-acetylglucosamine--N-acetylmuramyl-  
             PSI-BLAST UDP-N-acetylglucosamine--N-acetylmuramyl-
        nr     (database updated : 2007.02.20 )
             BLAST Chain A, The 1.9 Angstrom Crystal Structure Of E. Coli Murg  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    MICROBODIES_CTER     

    Other Cross-Reference
        COG COG0707M 
        EcoCyc murG 

    MMBR References
    Nucleic Acids Res. 1990;18(13):4014
    Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli.
    Ikeda M, Wachi M, Jung HK, Ishino F, Matsuhashi M. ( 2197603 )
    J Bacteriol. 1989;171(11):6379-82.
    Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes.
    Jung HK, Ishino F, Matsuhashi M. ( 2553679 )
    J Bacteriol. 1989;171(11):6126-34.
    Organization of the murE-murG region of Escherichia coli: identification of the murD gene encoding the D-glutamic-acid-adding enzyme.
    Mengin-Lecreulx D, Parquet C, Desviat LR, Pla J, Flouret B, Ayala JA, van Heijenoort J. ( 2681153 )
    J Bacteriol. 1991;173(15):4625-36.
    The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis.
    Mengin-Lecreulx D, Texier L, Rousseau M, van Heijenoort J. ( 1649817 )
    J Bacteriol. 1980;144(1):438-40.
    Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell envelope gene murG.
    Salmond GP, Lutkenhaus JF, Donachie WD. ( 6998962 )

    Sequences
    Amino acid
    FASTA format 
    0001 MSGQGKRLMV MAGGTGGHVF PGLAVAHHLM AQGWQVRWLG TADRMEADLV PKHGIEIDFI RISGLRGKGI 
0071 KALIAAPLRI FNAWRQARAI MKAYKPDVVL GMGGYVSGPG GLAAWSLGIP VVLHEQNGIA GLTNKWLAKI 
0141 ATKVMQAFPG AFPNAEVVGN PVRTDVLALP LPQQRLAGRE GPVRVLVVGG SQGARILNQT MPQVAAKLGD 
0211 SVTIWHQSGK GSQQSVEQAY AEAGQPQHKV TEFIDDMAAA YAWADVVVCR SGALTVSEIA AAGLPALFVP 
0281 FQHKDRQQYW NALPLEKAGA AKIIEQPQLS VDAVANTLAG WSRETLLTMA ERARAASIPD ATERVANEVS 
0351 RVARA
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             GTTCGAGCTT ACTGATTATG TCGACAGCCA 
-070 TCATGATGCT GTTGCGTATT GATTATGAAA CGCGTCTGGA GAAAGCGCAG GCGTTTGTAC GAGGTTCACG 
0001 atgagtggtc aaggaaagcg attaatggtg atggcaggcg gaaccggtgg acatgtattc ccgggactgg 
0071 cggttgcgca ccatctaatg gctcagggtt ggcaagttcg ctggctgggg actgccgacc gtatggaagc 
0141 ggacttagtg ccaaaacatg gcatcgaaat tgatttcatt cgtatctctg gtctgcgtgg aaaaggtata 
0211 aaagcactga tagctgcccc gctgcgtatc ttcaacgcct ggcgtcaggc gcgggcgatt atgaaagcgt 
0281 acaaacctga cgtggtgctc ggtatgggag gctacgtgtc aggtccaggt ggtctggccg cgtggtcgtt 
0351 aggcattccg gttgtacttc atgaacaaaa cggtattgcg ggcttaacca ataaatggct ggcgaagatt 
0421 gccaccaaag tgatgcaggc gtttccaggt gctttcccta atgcggaagt agtgggtaac ccggtgcgta 
0491 ccgatgtgtt ggcgctgccg ttgccgcagc aacgtttggc tggacgtgaa ggtccggttc gtgtgctggt 
0561 agtgggtggt tctcagggcg cacgcattct taaccagaca atgccgcagg ttgctgcgaa actgggtgat 
0631 tcagtcacta tctggcatca gagcggcaaa ggttcgcaac aatccgttga acaggcgtat gccgaagcgg 
0701 ggcaaccgca gcataaagtg acggaattta ttgatgatat ggcggcggcg tatgcgtggg cggatgtcgt 
0771 cgtttgccgc tccggtgcgt taacggtgag tgaaatcgcc gcggcaggac taccggcgtt gtttgtgccg 
0841 tttcaacata aagaccgcca gcaatactgg aatgcgctac cgctggaaaa agcgggcgca gccaaaatta 
0911 tcgagcagcc acagcttagc gtggatgctg tcgccaacac cctggccggg tggtcgcgag aaaccttatt 
0981 aaccatggca gaacgcgccc gcgctgcatc cattccggat gccaccgagc gagtggcaaa tgaagtgagc 
1051 cgggttgccc gggcgtaaTT GTAGCGATGC CTTTTGCATC GTATGAATTT AAGAAGTTAA TGGCGTAAAG 
1121 AATGAATACA CAACAATTGG CAAAACTGCG TTCCATCGTG CCCGAAAT