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PEC Original Annotations
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Essentiality
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Class
|
non-essential
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References (PMID)
|
J Bacteriol. 1990;172(11):6518-28.
Metabolic relationships between pyridoxine (vitamin B6) and serine biosynthesis in Escherichia coli K-12.
Lam HM, Winkler ME.
(
2121717
)
|
|
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Deletion
|
OCR52
(D)
|
|
Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
serC
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Related strains
|
|
Strains Search
|
Search strains by gene name:
serC
Search strains by all related name:
serC b0907 ECK0898 JW0890 pdxC pdxF
|
General information (Go to Linear View: )
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Gene Name
|
serC
|
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Alternative name
|
b0907,ECK0898,JW0890,pdxC,pdxF
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Location, Length
|
956,876 - 957,964
(
+
)
;
20.62
min
;
1089
(bp) ,
362
(aa)
|
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Product
|
3-phosphoserine/phosphohydroxythreonine aminotransferase
|
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Operon Name
|
serC-aroA
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Note
|
3-phosphoserine aminotransferase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006564 - L-serine biosynthetic process
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Function
|
enzyme; Amino acid biosynthesis: Serine
|
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Gene Ontology
|
GO:0003824
;
catalytic activity ( serC )
GO:0004648
;
phosphoserine transaminase activity ( serC )
GO:0005515
;
protein binding ( serC )
GO:0005737
;
cytoplasm ( serC )
GO:0006564
;
L-serine biosynthetic process ( serC )
GO:0008152
;
metabolic process ( serC )
GO:0008483
;
transaminase activity ( serC )
GO:0008615
;
pyridoxine biosynthetic process ( serC )
GO:0008652
;
amino acid biosynthetic process ( serC )
GO:0016740
;
transferase activity ( serC )
GO:0030170
;
pyridoxal phosphate binding ( serC )
|
|
PID
|
1787136
|
EC number
(KEGG Pathway)
|
2.6.1.52
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
serC
(
EG10946
)
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Number of removed
|
1 aa cleaved
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SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
SERC_ECOLI
(
P23721
;
P78266
)
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- Protein name
|
Phosphoserine aminotransferase
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- Synonyms
|
EC 2.6.1.52, PSAT
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- Gene name
|
Name=serC; Synonyms=pdxF; OrderedLocusNames=b0907;
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- Comments
|
CATALYTIC ACTIVITY:O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate.
COFACTOR:Pyridoxal phosphate.
PATHWAY:Required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine.
SUBUNIT:Homodimer.
SUBCELLULAR LOCATION:Cytoplasmic.
SIMILARITY:Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
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Entry name(Acc.no)
|
SERC_ECOL6
(
Q8FJB7
)
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- Protein name
|
Phosphoserine aminotransferase
|
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- Synonyms
|
EC 2.6.1.52, PSAT
|
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- Gene name
|
Name=serC; OrderedLocusNames=c1045;
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- Comments
|
CATALYTIC ACTIVITY:O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate.
COFACTOR:Pyridoxal phosphate (By similarity).
PATHWAY:Required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine (By similarity).
SUBUNIT:Homodimer (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
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Entry name(Acc.no)
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SERC_ECO57
(
Q8XEA7
)
|
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- Protein name
|
Phosphoserine aminotransferase
|
|
- Synonyms
|
EC 2.6.1.52, PSAT
|
|
- Gene name
|
Name=serC; OrderedLocusNames=z1253, ECs0990;
|
|
- Comments
|
CATALYTIC ACTIVITY:O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate.
COFACTOR:Pyridoxal phosphate (By similarity).
PATHWAY:Required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine (By similarity).
SUBUNIT:Homodimer (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
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Homology Analysis
|
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BLAST
|
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Bacteria
|
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GTOP
|
serC
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain A, Phosphoserine Aminotransferase From Bacillus Circulans Subsp. Alkalophilus
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Phosphoserine aminotransferase
|
|
PSI-BLAST
|
Serine hydroxymethyltransferase
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
phosphoserine aminotransferase [Escherichia coli K12]
|
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Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
|
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AA_TRANSFER_CLASS_5
|
|
MMBR References
|
J Bacteriol. 1970;104(2):658-67.
Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants.
Dempsey WB, Ito H.
(
4321330
)
|
J Bacteriol. 1971;107(2):580-2.
Identification of the forms of vitamin B 6 present in the culture media of "vitamin B 6 control" mutants.
Dempsey WB, Arcement LJ.
(
4939769
)
|
Biochem J. 1986;234(1):49-57.
The serC-aroA operon of Escherichia coli. A mixed function operon encoding enzymes from two different amino acid biosynthetic pathways.
Duncan K, Coggins JR.
(
3518706
)
|
Mol Gen Genet. 1984;193(1):72-5.
Construction and analysis of plasmids containing the Escherichia coli serB gene.
Garnant MK, Stauffer GV.
(
6318053
)
|
Nucleic Acids Res. 1985;13:7025-7039
DNA sequence and characterization of the Escherichia coli serB gene.
Neuwald, A. F., and G. V. Stauffer.
|
J Bacteriol. 1978;134(3):944-9.
3-hydroxypyruvate substitutes for pyridoxine in serC mutants of Escherichia coli K-12.
Shimizu S, Dempsey WB.
(
350858
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MAQIFNFSSG PAMLPAEVLK QAQQELRDWN GLGTSVMEVS HRGKEFIQVA EEAEKDFRDL LNVPSNYKVL
0071 FCHGGGRGQF AAVPLNILGD KTTADYVDAG YWAASAIKEA KKYCTPNVFD AKVTVDGLRA VKPMREWQLS
0141 DNAAYMHYCP NETIDGIAID ETPDFGADVV VAADFSSTIL SRPIDVSRYG VIYAGAQKNI GPAGLTIVIV
0211 REDLLGKANI ACPSILDYSI LNDNGSMFNT PPTFAWYLSG LVFKWLKANG GVAEMDKINQ QKAELLYGVI
0281 DNSDFYRNDV AKANRSRMNV PFQLADSALD KLFLEESFAA GLHALKGHRV VGGMRASIYN AMPLEGVKAL
0351 TDFMVEFERR HG
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GTCACTGAAT GATAAAACCG ATAGCCACAG
-070 GAATAATGTA TTACCTGTGG TCGCAATCGA TTGACCGCGG GTTAATAGCA ACGCAACGTG GTGAGGGGAA
0001 atggctcaaa tcttcaattt tagttctggt ccggcaatgc taccggcaga ggtgcttaaa caggctcaac
0071 aggaactgcg cgactggaac ggtcttggta cgtcggtgat ggaagtgagt caccgtggca aagagttcat
0141 tcaggttgca gaggaagccg agaaggattt tcgcgatctt cttaatgtcc cctccaacta caaggtatta
0211 ttctgccatg gcggtggtcg cggtcagttt gctgcggtac cgctgaatat tctcggtgat aaaaccaccg
0281 cagattatgt tgatgccggt tactgggcgg caagtgccat taaagaagcg aaaaaatact gcacgcctaa
0351 tgtctttgac gccaaagtga ctgttgatgg tctgcgcgcg gttaagccaa tgcgtgaatg gcaactctct
0421 gataatgctg cttatatgca ttattgcccg aatgaaacca tcgatggtat cgccatcgac gaaacgccag
0491 acttcggcgc agatgtggtg gtcgccgctg acttctcttc aaccattctt tcccgtccga ttgacgtcag
0561 ccgttatggt gtaatttacg ctggcgcgca gaaaaatatc ggcccggctg gcctgacaat cgtcatcgtt
0631 cgtgaagatt tgctgggcaa agcgaatatc gcgtgtccgt cgattctgga ttattccatc ctcaacgata
0701 acggctccat gtttaacacg ccgccgacat ttgcctggta tctatctggt ctggtcttta aatggctgaa
0771 agcgaacggc ggtgtagctg aaatggataa aatcaatcag caaaaagcag aactgctata tggggtgatt
0841 gataacagcg atttctaccg caatgacgtg gcgaaagcta accgttcgcg gatgaacgtg ccgttccagt
0911 tggcggacag tgcgcttgac aaattgttcc ttgaagagtc ttttgctgct ggccttcatg cactgaaagg
0981 tcaccgtgtg gtcggcggaa tgcgcgcttc tatttataac gccatgccgc tggaaggcgt taaagcgctg
1051 acagacttca tggttgagtt cgaacgccgt cacggttaaT GCCGAAATTT TGCTTAATCC CCACAGCCAG
1121 CCTGTGGGGT TTTTATTTCT GTTGTAGAGA GTTGAGTTCA TGGAATCCCT GACGTTACAA CCCATCGCT
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