MG1655
W3110
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Gene Report : mraY
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1998;180(23):6429-32.
mraY is an essential gene for cell growth in Escherichia coli.
Boyle DS, Donachie WD. ( 9829961 )
     Deletion OCR30,31-3 (BK2-1 / AK1-1) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  mraY
Related strains
     Strains Search Search strains by gene name:  mraY   Search strains by all related name:  mraY b0087 ECK0088 JW0085 murX

General information  (Go to Linear View:)
 Gene Name mraY  
 Alternative name b0087,ECK0088,JW0085,murX  
 Location, Length 96,002 - 97,084 (  +  ) ;   2.07 min ; 1083 (bp) ,   360 (aa) Go to Linear View
 Product phospho-N-acetylmuramoyl-pentapeptide transferase  
 Operon Name mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC  
 Note phospho-N-acetylmuramoyl-pentapeptide transferase?; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009252 - peptidoglycan biosynthetic process  
 Function enzyme; Murein sacculus, peptidoglycan  
 Gene Ontology GO:0000287 ; magnesium ion binding ( mraY )
GO:0005886 ; plasma membrane ( mraY )
GO:0007047 ; cell wall organization and biogenesis ( mraY )
GO:0007049 ; cell cycle ( mraY )
GO:0008360 ; regulation of cell shape ( mraY )
GO:0008963 ; phospho-N-acetylmuramoyl-pentapeptide-transferase activity ( mraY )
GO:0009252 ; peptidoglycan biosynthetic process ( mraY )
GO:0016020 ; membrane ( mraY )
GO:0016021 ; integral to membrane ( mraY )
GO:0016740 ; transferase activity ( mraY )
GO:0030145 ; manganese ion binding ( mraY )
GO:0051301 ; cell division ( mraY )
 PID 1786275  
 EC number
  (KEGG Pathway)   		2.7.8.13  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) MRAY_ECOLI ( P15876 )
    -  Protein name Phospho-N-acetylmuramoyl-pentapeptide-transferase  
    -  Synonyms EC 2.7.8.13, UDP- MurNAc-pentapeptide phosphotransferase  
    -  Gene name Name=mraY; Synonyms=murX; OrderedLocusNames=b0087, SF0084, S0086;  
    -  Comments
  • FUNCTION:First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan.
  • CATALYTIC ACTIVITY:UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol.
  • COFACTOR:Magnesium or manganese.
  • PATHWAY:Peptidoglycan biosynthesis.
  • SUBCELLULAR LOCATION:Integral membrane protein.
  • SIMILARITY:Belongs to the glycosyltransferase family 4. MraY subfamily.  
    •   botton Entry name(Acc.no) MRAY_ECOL6 ( Q8X9Z0 )
        -  Protein name Phospho-N-acetylmuramoyl-pentapeptide-transferase  
        -  Synonyms EC 2.7.8.13, UDP- MurNAc-pentapeptide phosphotransferase  
        -  Gene name Name=mraY; OrderedLocusNames=c0105, z0097, ECs0091;  
        -  Comments
  • FUNCTION:First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan (By similarity).
  • CATALYTIC ACTIVITY:UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol.
  • PATHWAY:Peptidoglycan biosynthesis.
  • SUBCELLULAR LOCATION:Integral membrane protein (By similarity).
  • SIMILARITY:Belongs to the glycosyltransferase family 4. MraY subfamily.  

    •  Linear View (Whole Mode)
    View Location
       85.0  –  110.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP mraY (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
            PSI-BLAST  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Phospho-N-acetylmuramoyl-pentapeptide-transferase (UDP-MurNAc-pentapeptide phosphotransferase)  
             PSI-BLAST Phospho-N-acetylmuramoyl-pentapeptide-transferase
        nr     (database updated : 2007.02.20 )
             BLAST phospho-N-acetylmuramoyl-pentapeptide-transferase [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    MYRISTYL     

    Other Cross-Reference
        COG COG0472M 
        EcoCyc mraY 

    MMBR References
    Nucleic Acids Res. 1990;18(4):1058
    Nucleotide sequence involving murD and an open reading frame ORF-Y spacing murF and ftsW in Escherichia coli.
    Ikeda M, Wachi M, Ishino F, Matsuhashi M. ( 2179861 )
    J Bacteriol. 1991;173(3):1021-6.
    The Escherichia coli mraY gene encoding UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase.
    Ikeda M, Wachi M, Jung HK, Ishino F, Matsuhashi M. ( 1846850 )

    Sequences
    Amino acid
    FASTA format 
    0001 MLVWLAEHLV KYYSGFNVFS YLTFRAIVSL LTALFISLWM GPRMIAHLQK LSFGQVVRND GPESHFSKRG 
0071 TPTMGGIMIL TAIVISVLLW AYPSNPYVWC VLVVLVGYGV IGFVDDYRKV VRKDTKGLIA RWKYFWMSVI 
0141 ALGVAFALYL AGKDTPATQL VVPFFKDVMP QLGLFYILLA YFVIVGTGNA VNLTDGLDGL AIMPTVFVAG 
0211 GFALVAWATG NMNFASYLHI PYLRHAGELV IVCTAIVGAG LGFLWFNTYP AQVFMGDVGS LALGGALGII 
0281 AVLLRQEFLL VIMGGVFVVE TLSVILQVGS FKLRGQRIFR MAPIHHHYEL KGWPEPRVIV RFWIISLMLV 
0351 LIGLATLKVR
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             AATTACTGAT TGCTGAGCAA CAGGTAATTA 
-070 CGATTTTAGT TAAGGGTTCA CGTAGTGCCG CCATGGAAGA GGTAGTACGC GCTTTACAGG AGAATGGGAC 
0001 atgttagttt ggctggccga acatttggtc aaatattatt ccggctttaa cgtcttttcc tatctgacgt 
0071 ttcgcgccat cgtcagcctg ctgaccgcgc tgttcatctc attgtggatg ggcccgcgta tgattgctca 
0141 tttgcaaaaa ctttcctttg gtcaggtggt gcgtaacgac ggtcctgaat cacacttcag caagcgcggt 
0211 acgccgacca tgggcgggat tatgatcctg acggcgattg tgatctccgt actgctgtgg gcttacccgt 
0281 ccaatccgta cgtctggtgc gtgttggtgg tgctggtagg ttacggtgtt attggctttg ttgatgatta 
0351 tcgcaaagtg gtgcgtaaag acaccaaagg gttgatcgct cgttggaagt atttctggat gtcggtcatt 
0421 gcgctgggtg tcgccttcgc cctgtacctt gccggcaaag acacgcccgc aacgcagctg gtggtcccat 
0491 tctttaaaga tgtgatgccg cagctggggc tgttctacat tctgctggct tacttcgtca ttgtgggtac 
0561 tggcaacgcg gtaaacctga ccgatggtct cgacggcctg gcaattatgc cgaccgtatt tgtcgccggt 
0631 ggttttgcgc tggtggcgtg ggcgaccggc aatatgaact ttgccagcta cttgcatata ccgtatctgc 
0701 gacacgccgg ggaactggtt attgtctgta ccgcgatagt cggggcagga ctgggcttcc tgtggtttaa 
0771 cacctatccg gcgcaggtct ttatgggcga tgtaggttcg ctggcgttag gtggtgcgtt aggcattatc 
0841 gccgtactgc tacgtcagga attcctgctg gtgattatgg ggggcgtgtt cgtggtagaa acgctttctg 
0911 tcatcctgca ggtcggctcc tttaaactgc gcggacaacg tattttccgc atggcaccga ttcatcacca 
0981 ctatgaactg aaaggctggc cggaaccgcg cgtcattgtg cgtttctgga ttatttcgct gatgctggtt 
1051 ctgattggtc tggcaacgct gaaggtacgt taaTCATGGC TGATTATCAG GGTAAAAATG TCGTCATTAT 
1121 CGGCCTGGGC CTCACCGGGC TTTCCTGCGT GGACTTTTTC CTCGCTCGCG GTGTGACGCC GCG