MG1655
W3110
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Gene Report : murF
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS. ( 9166795 )
     Deletion OCR30,31-3 (BK2-1 / AK1-1) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  murF
Related strains
     Strains Search Search strains by gene name:  murF   Search strains by all related name:  murF b0086 ECK0087 JW0084 mra

General information  (Go to Linear View:)
 Gene Name murF  
 Alternative name b0086,ECK0087,JW0084,mra  
 Location, Length 94,650 - 96,008 (  +  ) ;   2.04 min ; 1359 (bp) ,   452 (aa) Go to Linear View
 Product UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase  
 Operon Name mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0000270 - peptidoglycan metabolic process  
 Function enzyme; Murein sacculus, peptidoglycan,1.6.7 metabolism; macromolecules (cellular constituent) biosynthesis; peptidoglycan (murein),1.7.34 metabolism; central intermediary metabolism; peptidoglycan (murein),1.7.34 metabolism; central intermediary metaboli  
 Gene Ontology GO:0000166; nucleotide binding ( murF )
GO:0005524; ATP binding ( murF )
GO:0005737; cytoplasm ( murF )
GO:0007047; cell wall organization and biogenesis ( murF )
GO:0007049; cell cycle ( murF )
GO:0008360; regulation of cell shape ( murF )
GO:0008766; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ( murF )
GO:0009058; biosynthetic process ( murF )
GO:0009252; peptidoglycan biosynthetic process ( murF )
GO:0009273; peptidoglycan-based cell wall biogenesis ( murF )
GO:0016874; ligase activity ( murF )
GO:0047480; UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity ( murF )
GO:0051301; cell division ( murF )
 PID 1786274  
 EC number
  (KEGG Pathway)
 
6.3.2.10  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene murF ( EG10622 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) MURF_ECOLI ( P11880 ; O07100; P77636 )
    -  Protein name UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase  
    -  Synonyms EC 6.3.2.10, UDP-MurNAc-pentapeptide synthetase, D-alanyl-D- alanine-adding enzyme  
    -  Gene name Name=murF; Synonyms=mra; OrderedLocusNames=b0086;  
    -  Comments
  • FUNCTION:Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.
  • CATALYTIC ACTIVITY:ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
  • PATHWAY:Peptidoglycan biosynthesis.
  • SUBUNIT:Monomer.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • SIMILARITY:Belongs to the murCDEF family.  
  •   botton Entry name(Acc.no) Q8FL66 ( Q8FL66 )
        -  Protein name UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl- D-alanyl ligase  
        -  Synonyms EC 6.3.2.15  
        -  Gene name Name=murF; OrderedLocusNames=c0104;  
        -  Comments
  • SIMILARITY:Belongs to the murCDEF family.  
  •   botton Entry name(Acc.no) Q8X9Z1 ( Q8X9Z1 )
        -  Protein name D-alanine:D-alanine-adding enzyme.  
        -  Synonyms  
        -  Gene name Name=murF; OrderedLocusNames=z0096;  
        -  Comments
  • SIMILARITY:Belongs to the murCDEF family.  

  •  Linear View (Whole Mode)
    View Location
       85.0  –  110.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP murF (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Crystal Structure Of Escherichia Coli Udpmurnac-Tripeptide D-Alanyl-D-Alanine-Adding Enzyme (Murf) At 2.3 Angstrom Resolution  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase  
             PSI-BLAST UDP-N-acetylmuramate--L-alanine ligase
        nr     (database updated : 2007.02.20 )
             BLAST UDP-MurNAc-Tripeptide:D-Ala-D-Ala-Adding Enzyme [Escherichia coli]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AA_TRNA_LIGASE_II_2     

    Other Cross-Reference
        COG COG0770M 
        EcoCyc murF 

    MMBR References
    Biochemistry. 1996;35(50):16264-9.
    Kinetic mechanism of the Escherichia coli UDPMurNAc-tripeptide D-alanyl-D-alanine-adding enzyme: use of a glutathione S-transferase fusion.
    Anderson MS, Eveland SS, Onishi HR, Pompliano DL. ( 8973200 )
    Biochemistry. 1997;36(20):6223-9.
    Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
    Eveland SS, Pompliano DL, Anderson MS. ( 9166795 )
    J Bacteriol. 1989;171(11):6379-82.
    Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes.
    Jung HK, Ishino F, Matsuhashi M. ( 2553679 )
    J Bacteriol. 1989;171(11):6126-34.
    Organization of the murE-murG region of Escherichia coli: identification of the murD gene encoding the D-glutamic-acid-adding enzyme.
    Mengin-Lecreulx D, Parquet C, Desviat LR, Pla J, Flouret B, Ayala JA, van Heijenoort J. ( 2681153 )
    Nucleic Acids Res. 1989;17(13):5379
    Nucleotide sequence of the murF gene encoding the UDP-MurNAc-pentapeptide synthetase of Escherichia coli.
    Parquet C, Flouret B, Mengin-Lecreulx D, van Heijenoort J. ( 2668880 )

    Sequences
    Amino acid
    FASTA format
    0001 MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA HDFADQAKAG GAGALLVSRP 
    0071 LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA RVVALTGSSG KTSVKEMTAA ILSQCGNTLY TAGNLNNDIG 
    0141 VPMTLLRLTP EYDYAVIELG ANHQGEIAWT VSLTRPEAAL VNNLAAAHLE GFGSLAGVAK AKGEIFSGLP 
    0211 ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS PNAANSDFTA TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR 
    0281 HNIANALAAA ALSMSVGATL DAIKAGLANL KAVPGRLFPI QLAENQLLLD DSYNANVGSM TAAVQVLAEM 
    0351 PGYRVLVVGD MAELGAESEA CHVQVGEAAK AAGIDRVLSV GKQSHAISTA SGVGEHFADK TALITRLKLL 
    0421 IAEQQVITIL VKGSRSAAME EVVRALQENG TC
    
    
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp
    -100                                             TGGTCGCGGG CAAAGGCCAT GAAGATTACC 
    -070 AGATTGTTGG CAATCAGCGT CTGGACTACT CCGATCGCGT CACGGTGGCG CGTCTGCTGG GGGTGATTGC 
    0001 atgattagcg taacccttag ccaacttacc gacattctca acggtgaact gcaaggtgca gatatcaccc 
    0071 ttgatgctgt aaccactgat acccgaaaac tgacgccggg ctgcctgttt gttgccctga aaggcgaacg 
    0141 ttttgatgcc cacgattttg ccgaccaggc gaaagctggc ggcgcaggcg cactactggt tagccgtccg 
    0211 ctggacatcg acctgccgca gttaatcgtc aaggatacgc gtctggcgtt tggtgaactg gctgcatggg 
    0281 ttcgccagca agttccggcg cgcgtggttg ctctgacggg gtcctccggc aaaacctccg ttaaagagat 
    0351 gacggcggcg attttaagcc agtgcggcaa cacgctttat acggcaggca atctcaacaa cgacatcggt 
    0421 gtaccgatga cgctgttgcg cttaacgccg gaatacgatt acgcagttat tgaacttggc gcgaaccatc 
    0491 agggcgaaat agcctggact gtgagtctga ctcgcccgga agctgcgctg gtcaacaacc tggcagcggc 
    0561 gcatctggaa ggttttggct cgcttgcggg tgtcgcgaaa gcgaaaggtg aaatctttag cggcctgccg 
    0631 gaaaacggta tcgccattat gaacgccgac aacaacgact ggctgaactg gcagagcgta attggctcac 
    0701 gcaaagtgtg gcgtttctca cccaatgccg ccaacagcga tttcaccgcc accaatatcc atgtgacctc 
    0771 gcacggtacg gaatttaccc tacaaacccc aaccggtagc gtcgatgttc tgctgccgtt gccggggcgt 
    0841 cacaatattg cgaatgcgct ggcagccgct gcgctctcca tgtccgtggg cgcaacgctt gatgctatca 
    0911 aagcggggct ggcaaatctg aaagctgttc caggccgtct gttccccatc caactggcag aaaaccagtt 
    0981 gctgctcgac gactcctaca acgccaatgt cggttcaatg actgcagcag tccaggtact ggctgaaatg 
    1051 ccgggctacc gcgtgctggt ggtgggcgat atggcggaac tgggcgctga aagcgaagcc tgccatgtac 
    1121 aggtgggcga ggcggcaaaa gctgctggta ttgaccgcgt gttaagcgtg ggtaaacaaa gccatgctat 
    1191 cagcaccgcc agcggcgttg gcgaacattt tgctgataaa actgcgttaa ttacgcgtct taaattactg 
    1261 attgctgagc aacaggtaat tacgatttta gttaagggtt cacgtagtgc cgccatggaa gaggtagtac 
    1331 gcgctttaca ggagaatggg acatgttagT TTGGCTGGCC GAACATTTGG TCAAATATTA TTCCGGCTTT 
    1401 AACGTCTTTT CCTATCTGAC GTTTCGCGCC ATCGTCAGCC TGCTGACCGC GCTGTTCAT