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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Eur J Biochem. 2000;267(3):666-76.
The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2Fe-2S].
van den Berg WA, Hagen WR, van Dongen WM.
(
10651802
)
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Deletion
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15
(LD)
,
15+dps
(LD)
,
OCR48,50-8 (LD 15D::Cm)
(D)
,
OCR49
(D)
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|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
hcp
|
|
Related strains
|
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Strains Search
|
Search strains by gene name:
hcp
Search strains by all related name:
hcp b0873 ECK0864 f552 JW0857 ybjW
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General information (Go to Linear View: )
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Gene Name
|
hcp
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Alternative name
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b0873,ECK0864,f552,JW0857,ybjW
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Location, Length
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911,385 - 913,037
(
-
)
;
19.64
min
;
1653
(bp) ,
550
(aa)
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Product
|
hybrid-cluster [4Fe-2S-2O] protein in anaerobic terminal reductases
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Operon Name
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hcp-hcr
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Note
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Function
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orf; Not classified
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Gene Ontology
|
GO:0003824
;
catalytic activity ( hcp )
GO:0005506
;
iron ion binding ( hcp )
GO:0005515
;
protein binding ( hcp )
GO:0005737
;
cytoplasm ( hcp )
GO:0006118
;
electron transport ( hcp )
GO:0016491
;
oxidoreductase activity ( hcp )
GO:0016661
;
oxidoreductase activity, acting on other nitrogenous compounds as donors ( hcp )
GO:0046872
;
metal ion binding ( hcp )
GO:0050418
;
hydroxylamine reductase activity ( hcp )
GO:0051536
;
iron-sulfur cluster binding ( hcp )
GO:0051537
;
2 iron, 2 sulfur cluster binding ( hcp )
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PID
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87081794
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
HCP_ECOLI
(
P75825
;
Q9R7R0; Q9R7R1
)
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- Protein name
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Hydroxylamine reductase
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- Synonyms
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EC 1.7.-.-, Hybrid-cluster protein, HCP
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- Gene name
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Name=hcp; OrderedLocusNames=b0873;
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- Comments
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FUNCTION:Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O. Is also able to reduce hydroxylamine analogs such as methylhydroxylamine and hydroxyquinone. Might have a role as a scavenger of potentially toxic by-products of nitrate metabolism.
COFACTOR:Binds 2 iron-sulfur clusters:1 2Fe-2S cluster and 1 hybrid 4Fe-2O-2S cluster.
ENZYME REGULATION:Inhibited by oxygen. Activated by cyanide except in the prolonged presence of excess cyanide, where the enzyme is inactivated.
SUBCELLULAR LOCATION:Cytoplasmic.
INDUCTION:Is expressed under anaerobic conditions in the presence of either nitrate or nitrite.
MISCELLANEOUS:Optimum pH for activity is 9.0.
SIMILARITY:Belongs to the HCP family.
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Entry name(Acc.no)
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HCP_ECOL6
(
Q8FJE0
)
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- Protein name
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Hydroxylamine reductase
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- Synonyms
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EC 1.7.-.-, Hybrid-cluster protein, HCP
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- Gene name
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Name=hcp; OrderedLocusNames=c1006;
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- Comments
|
FUNCTION:Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O (By similarity).
COFACTOR:Binds 2 iron-sulfur clusters:1 2Fe-2S cluster and 1 hybrid 4Fe-2O-2S cluster (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the HCP family.
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Entry name(Acc.no)
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HCP_ECO57
(
Q8X6L0
)
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- Protein name
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Hydroxylamine reductase
|
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- Synonyms
|
EC 1.7.-.-, Hybrid-cluster protein, HCP
|
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- Gene name
|
Name=hcp; OrderedLocusNames=z1107, ECs0959;
|
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- Comments
|
FUNCTION:Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O (By similarity).
COFACTOR:Binds 2 iron-sulfur clusters:1 2Fe-2S cluster and 1 hybrid 4Fe-2O-2S cluster (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the HCP family.
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| Sequences |
Amino acid
FASTA format
|
0001 MFCVQCEQTI RTPAGNGCSY AQGMCGKTAE TSDLQDLLIA ALQGLSAWAV KAREYGIINH DVDSFAPRAF
0071 FSTLTNVNFD SPRIVGYARE AIALREALKA QCLAVDANAR VDNPMADLQL VSDDLGELQR QAAEFTPNKD
0141 KAAIGENILG LRLLCLYGLK GAAAYMEHAH VLGQYDNDIY AQYHKIMAWL GTWPADMNAL LECSMEIGQM
0211 NFKVMSILDA GETGKYGHPT PTQVNVKATA GKCILISGHD LKDLYNLLEQ TEGTGVNVYT HGEMLPAHGY
0281 PELRKFKHLV GNYGSGWQNQ QVEFARFPGP IVMTSNCIID PTVGAYDDRI WTRSIVGWPG VRHLDGDDFS
0351 AVITQAQQMA GFPYSEIPHL ITVGFGRQTL LGAADTLIDL VSREKLRHIF LLGGCDGARG ERHYFTDFAT
0421 SVPDDCLILT LACGKYRFNK LEFGDIEGLP RLVDAGQCND AYSAIILAVT LAEKLGCGVN DLPLSLVLSW
0491 FEQKAIVILL TLLSLGVKNI VTGPTAPGFL TPDLLAVLNE KFGLRSITTV EEDMKQLLSA
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tcaatctccc ttaaagttgc atgaaaaatc
-070 ccttttatcc ccgcgttaag cgtcttaacc ttaaacatgt atattaaata taactttaaa aggtgtgatc
0001 atgttttgtg tgcaatgtga acaaactatc cgtactccgg caggaaacgg ctgctcatac gcgcagggga
0071 tgtgtggtaa aacggcggaa acttctgacc ttcaggattt actcatcgcg gcgctgcaag ggctttctgc
0141 ctgggcggta aaagcgcgtg aatacggcat catcaaccac gatgttgaca gcttcgcgcc acgtgccttt
0211 ttctcaaccc tgaccaacgt taacttcgat tctccgcgta ttgtcggcta cgctcgtgaa gcgattgccc
0281 tgcgcgaggc gctgaaagcg caatgcctgg ctgtagatgc caacgcgcgc gtcgataacc cgatggctga
0351 cctgcaactg gtgagcgacg atctcggcga gctgcaacgt caggcagcag aatttacccc taacaaagat
0421 aaagcggcga ttggcgaaaa cattctcggc ctgcgtctgc tgtgcctgta tggcctgaaa ggtgcggcgg
0491 cctatatgga acacgcgcat gttctcggtc aatacgacaa cgatatttat gcccagtacc ataaaatcat
0561 ggcgtggctg gggacctggc ctgccgatat gaacgcgctt cttgagtgtt caatggaaat cggccagatg
0631 aacttcaaag tgatgagcat tctggatgca ggcgaaaccg gtaaatacgg tcacccgacg ccaacccagg
0701 tcaacgtcaa agcgacggcg ggtaaatgca ttctgatttc cggtcacgat ctcaaagatc tctacaacct
0771 gctggaacag accgaaggca cgggcgttaa tgtctacacc cacggcgaaa tgctgcctgc gcatggctac
0841 ccggagctgc gtaaattcaa gcatctggtc ggtaactacg gcagcggctg gcagaatcag caagtggagt
0911 tcgctcgttt ccctggcccc atcgtgatga cctcgaactg catcatcgac ccaaccgtag gcgcttatga
0981 cgatcgtatc tggacccgca gcattgttgg ctggcctggc gtgcgtcatc tggacggtga tgatttctct
1051 gcggttatca cccaggcgca acagatggca ggcttcccgt acagcgaaat tccgcacctt atcaccgtgg
1121 gttttggtcg ccagacgctg cttggcgctg ctgatacgct gattgatctg gtgagccgtg aaaaactgcg
1191 tcatatcttc ctgcttggtg gctgtgacgg cgcacgcggc gagcgccact acttcaccga tttcgccacc
1261 agcgtgccgg atgactgcct gatcctgacc ctcgcctgtg gtaaatatcg ctttaacaaa cttgagtttg
1331 gcgatatcga aggtctgccg cgtctggtag atgcaggtca atgtaacgat gcttactcag cgattattct
1401 ggctgtcact ctggcagaga aactgggctg cggtgtcaac gatctgccgc tgtcgctggt gctctcctgg
1471 tttgaacaga aagcgatcgt cattctgctg acgctgcttt ctctgggcgt gaaaaatatc gtcaccggcc
1541 cgactgcacc tggtttcctg acaccagacc tgctggcggt gctgaacgag aaattcggcc tgcgttctat
1611 caccactgtt gaagaagaca tgaagcaact gttgagcgcg taaggagttt gtcgatgacg atgccaacga
1681 atcaatgccc gtggcggatg caggttcatc acattacgca agaaacgccg gatgtgtgga cgatttccct
1751 gat
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