MG1655
W3110
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Gene Report : leuO
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR30 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  leuO
Related strains
     Strains Search Search strains by gene name:  leuO   Search strains by all related name:  leuO b0076 ECK0078 JW0075

General information  (Go to Linear View:)
 Gene Name leuO  
 Alternative name b0076,ECK0078,JW0075  
 Location, Length 84,368 - 85,312 (  +  ) ;   1.82 min ; 945 (bp) ,   314 (aa) Go to Linear View
 Product DNA-binding transcriptional activator  
 Operon Name leuO  
 Note probable transcriptional activator for leuABCD operon; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_process: GO:0009098 - leucine biosynthetic process; GO_process: GO:0006350 - transcription  
 Function putative regulator; Amino acid biosynthesis: Leucine  
 Gene Ontology GO:0003677; DNA binding ( leuO )
GO:0003700; transcription factor activity ( leuO )
GO:0006350; transcription ( leuO )
GO:0006355; regulation of transcription, DNA-dependent ( leuO )
 PID 87081684  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) LEUO_ECOLI ( P10151 ; P75640 )
    -  Protein name Probable HTH-type transcriptional regulator leuO.  
    -  Synonyms  
    -  Gene name Name=leuO; OrderedLocusNames=b0076;  
    -  Comments
  • SIMILARITY:Contains 1 HTH lysR-type DNA-binding domain.  
  •   botton Entry name(Acc.no) Q8FL73 ( Q8FL73 )
        -  Protein name Probable activator protein in leuABCD operon.  
        -  Synonyms  
        -  Gene name Name=leuO; OrderedLocusNames=c0093;  
        -  Comments
  • SIMILARITY:Contains 1 HTH lysR-type DNA-binding domain.  
  •   botton Entry name(Acc.no) Q8X9Z7 ( Q8X9Z7 )
        -  Protein name Probable transcriptional activator for leuABCD operon.  
        -  Synonyms  
        -  Gene name Name=leuO; OrderedLocusNames=z0086;  
        -  Comments
  • SIMILARITY:Contains 1 HTH lysR-type DNA-binding domain.  

  •  Linear View (Whole Mode)
    View Location
       75.0  –  100.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP leuO (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Dntr From Burkholderia Sp. Strain Dnt In Complex With Thiocyanate  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Probable HTH-type transcriptional regulator leuO  
             PSI-BLAST Probable HTH-type transcriptional regulator leuO
        nr     (database updated : 2007.02.20 )
             BLAST COG0583: Transcriptional regulator [Escherichia coli 101-1]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    HTH_LYSR_FAMILY    AA_TRNA_LIGASE_II_2     

    Other Cross-Reference
        COG COG0583K 
        EcoCyc leuO 

    MMBR References
    Mol Microbiol. 1997;25(3):559-69.
    The LysR-like regulator LeuO in Escherichia coli is involved in the translational regulation of rpoS by affecting the expression of the small regulatory DsrA-RNA.
    Klauck E, Bohringer J, Hengge-Aronis R. ( 9302018 )
    J Bacteriol. 1995;177:810-814
    Effects of multicopy LeuO on the expression of the acid-inducible lysine decarboxylase gene in Escherichia coli.
    Shi, X., and G. N. Bennett.
    J Bacteriol. 1998;180:190-193
    The leuO gene product has a latent ability to relieve bgl silencing in Escherichia coli.
    Ueguchi, C., T. Ohta, C. Seto, T. Suzuki, and T. Mizuno.

    Sequences
    Amino acid
    FASTA format
    0001 MPEVQTDHPE TAELSKPQLR MVDLNLLTVF DAVMQEQNIT RAAHVLGMSQ PAVSNAVARL KVMFNDELFV 
    0071 RYGRGIQPTA RAFQLFGSVR QALQLVQNEL PGSGFEPASS ERVFHLCVCS PLDSILTSQI YNHIEQIAPN 
    0141 IHVMFKSSLN QNTEHQLRYQ ETEFVISYED FHRPEFTSVP LFKDEMVLVA SKNHPTIKGP LLKHDVYNEQ 
    0211 HAAVSLDRFA SFSQPWYDTV DKQASIAYQG MAMMSVLSVV SQTHLVAIAP RWLAEEFAES LELQVLPLPL 
    0281 KQNSRTCYLS WHEAAGRDKG HQWMEEQLVS ICKR
    
    
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp
    -100                                             CTTTTTCTTA TTTTATATGC ATGATAAATC 
    -070 ATATTCTTCA GGATTATTTC TCTGCATTCC AATAAGGGAA AGGGAGTTAA GTGTGACAGT GGAGTTAAGT 
    0001 atgccagagg tacaaacaga tcatccagag acggcggagt taagcaaacc acagctacgc atggtcgatc 
    0071 tcaacttatt aaccgttttc gatgccgtga tgcaggagca aaacattact cgtgccgctc atgttctggg 
    0141 aatgtcgcaa cctgcggtca gtaacgctgt tgcacgcctg aaggtgatgt ttaatgacga gctttttgtt 
    0211 cgttatggcc gtggtattca accgactgct cgcgcatttc aactttttgg ttcagttcgt caggcattgc 
    0281 aactagtaca aaatgaattg cctggttcag gttttgaacc cgcgagcagt gaacgtgtat ttcatctttg 
    0351 tgtttgcagc ccgttagaca gcattctgac ctcgcagatt tataatcaca ttgagcagat tgcgccaaat 
    0421 atacatgtta tgttcaagtc ttcattaaat cagaacactg aacatcagct gcgttatcag gaaacggagt 
    0491 ttgtgattag ttatgaagac ttccatcgtc ctgaatttac cagcgtacca ttatttaaag atgaaatggt 
    0561 gctggtagcc agcaaaaatc atccaacaat taagggcccg ttactgaaac atgatgttta taacgaacaa 
    0631 catgcggcgg tttcgctcga tcgtttcgcg tcatttagtc aaccttggta tgacacggta gataagcaag 
    0701 ccagtatcgc gtatcagggc atggcaatga tgagcgtact tagcgtggtg tcgcaaacgc atttggtcgc 
    0771 tattgcgccg cgttggctgg ctgaagagtt cgctgaatcc ttagaattac aggtattacc gctgccgtta 
    0841 aaacaaaaca gcagaacctg ttatctctcc tggcatgaag ctgccgggcg cgataaaggc catcagtgga 
    0911 tggaagagca attagtctca atttgcaaac gctaaCTGAT TGCAGAATAG GTCAGACATG AATGTCTGGT 
    0981 TTATTCTGCA TTTTTTATTG AATGTAGAAT TTTATTCTGA ATGTGTGGGC TCTCTATTTT AGGAT