MG1655
W3110
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Gene Report : leuB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR30 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  leuB
Related strains
     Strains Search Search strains by gene name:  leuB   Search strains by all related name:  leuB b0073 ECK0075 JW5807

General information  (Go to Linear View:)
 Gene Name leuB  
 Alternative name b0073,ECK0075,JW5807  
 Location, Length 80,867 - 81,958 (  -  ) ;   1.74 min ; 1092 (bp) ,   363 (aa) Go to Linear View
 Product 3-isopropylmalate dehydrogenase, NAD(+)-dependent  
 Operon Name leuLABCD  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009098 - leucine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Leucine  
 Gene Ontology GO:0000287 ; magnesium ion binding ( leuB )
GO:0003862 ; 3-isopropylmalate dehydrogenase activity ( leuB )
GO:0005737 ; cytoplasm ( leuB )
GO:0008152 ; metabolic process ( leuB )
GO:0008652 ; amino acid biosynthetic process ( leuB )
GO:0009082 ; branched chain family amino acid biosynthetic process ( leuB )
GO:0009098 ; leucine biosynthetic process ( leuB )
GO:0016491 ; oxidoreductase activity ( leuB )
GO:0016616 ; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor ( leuB )
GO:0030145 ; manganese ion binding ( leuB )
GO:0046872 ; metal ion binding ( leuB )
 PID 87081683  
 EC number
  (KEGG Pathway)   		1.1.1.85  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene leuB ( EG11577 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) LEU3_ECOLI ( P30125 ; P78043 )
    -  Protein name 3-isopropylmalate dehydrogenase  
    -  Synonyms EC 1.1.1.85, Beta-IPM dehydrogenase, IMDH, 3-IPM-DH  
    -  Gene name Name=leuB; OrderedLocusNames=b0073;  
    -  Comments
  • FUNCTION:Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
  • CATALYTIC ACTIVITY:3-carboxy-2-hydroxy-4-methylpentanoate + NAD(+) = 3-carboxy-4-methyl-2-oxopentanoate + NADH.
  • PATHWAY:Leucine biosynthesis; third step.
  • SUBUNIT:Homodimer.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • SIMILARITY:Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB subfamily 1.  
    •   botton Entry name(Acc.no) LEU3_ECOL6 ( Q8FL76 )
        -  Protein name 3-isopropylmalate dehydrogenase  
        -  Synonyms EC 1.1.1.85, Beta-IPM dehydrogenase, IMDH, 3-IPM-DH  
        -  Gene name Name=leuB; OrderedLocusNames=c0090;  
        -  Comments
  • FUNCTION:Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
  • CATALYTIC ACTIVITY:3-carboxy-2-hydroxy-4-methylpentanoate + NAD(+) = 3-carboxy-4-methyl-2-oxopentanoate + NADH.
  • PATHWAY:Leucine biosynthesis; third step.
  • SUBUNIT:Homodimer (By similarity).
  • SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
  • SIMILARITY:Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB subfamily 1.  
    •   botton Entry name(Acc.no) LEU3_ECO57 ( Q8X9Z9 )
        -  Protein name 3-isopropylmalate dehydrogenase  
        -  Synonyms EC 1.1.1.85, Beta-IPM dehydrogenase, IMDH, 3-IPM-DH  
        -  Gene name Name=leuB; OrderedLocusNames=z0082, ECs0077;  
        -  Comments
  • FUNCTION:Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
  • CATALYTIC ACTIVITY:3-carboxy-2-hydroxy-4-methylpentanoate + NAD(+) = 3-carboxy-4-methyl-2-oxopentanoate + NADH.
  • PATHWAY:Leucine biosynthesis; third step.
  • SUBUNIT:Homodimer (By similarity).
  • SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
  • SIMILARITY:Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB subfamily 1.  

    •  Linear View (Whole Mode)
    View Location
       70.0  –  95.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP leuB (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, 3-Isopropylmalate Dehydrogenase From Escherichia Coli  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST 3-isopropylmalate dehydrogenase  
             PSI-BLAST 3-isopropylmalate dehydrogenase
        nr     (database updated : 2007.02.20 )
             BLAST COG0473: Isocitrate/isopropylmalate dehydrogenase [Escherichia coli E24377A]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    IDH_IMDH     

    Other Cross-Reference
        COG COG0473CE 
        EcoCyc leuB 

    MMBR References
    J Bacteriol. 1977;129(2):1078-90.
    Isolation and characterization of lambda pleu bacteriophages.
    Davis MG, Calvo JM. ( 320178 )

    Sequences
    Amino acid
    FASTA format 
    0001 MSKNYHIAVL PGDGIGPEVM TQALKVLDAV RNRFAMRITT SHYDVGGAAI DNHGQPLPPA TVEGCEQADA 
0071 VLFGSVGGPK WEHLPPDQQP ERGALLPLRK HFKLFSNLRP AKLYQGLEAF CPLRADIAAN GFDILCVREL 
0141 TGGIYFGQPK GREGSGQYEK AFDTEVYHRF EIERIARIAF ESARKRRHKV TSIDKANVLQ SSILWREIVN 
0211 EIATEYPDVE LAHMYIDNAT MQLIKDPSQF DVLLCSNLFG DILSDECAMI TGSMGMLPSA SLNEQGFGLY 
0281 EPAGGSAPDI AGKNIANPIA QILSLALLLR YSLDADDAAC AIERAINRAL EEGIRTGDLA RGAAAVSTDE 
0351 MGDIIARYVA EGV
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             ccatggtgca cgttctgaac aatatctggc 
-070 gtgccgcaga agtcgaaaaa gagttgcaac gcaaagctca acacaacgaa aacaacaagg aaaccgtgtg 
0001 atgtcgaaga attaccatat tgccgtattg ccgggggacg gtattggtcc ggaagtgatg acccaggcgc 
0071 tgaaagtgct ggatgccgtg cgcaaccgct ttgcgatgcg catcaccacc agccattacg atgtaggcgg 
0141 cgcagccatt gataaccacg ggcaaccact gccgcctgcg acggttgaag gttgtgagca agccgatgcc 
0211 gtgctgtttg gctcggtagg cggcccgaag tgggaacatt taccaccaga ccagcaacca gaacgcggcg 
0281 cgctgctgcc tctgcgtaag cacttcaaat tattcagcaa cctgcgcccg gcaaaactgt atcaggggct 
0351 ggaagcattc tgtccgctgc gtgcagacat tgccgcaaac ggcttcgaca tcctgtgtgt gcgcgaactg 
0421 accggcggca tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca atatgaaaaa gcctttgata 
0491 ccgaggtgta tcaccgtttt gagatcgaac gtatcgcccg catcgcgttt gaatctgctc gcaagcgtcg 
0561 ccacaaagtg acgtcgatcg ataaagccaa cgtgctgcaa tcctctattt tatggcggga gatcgttaac 
0631 gagatcgcca cggaataccc ggatgtcgaa ctggcgcata tgtacatcga caacgccacc atgcagctga 
0701 ttaaagatcc atcacagttt gacgttctgc tgtgctccaa cctgtttggc gacattctgt ctgacgagtg 
0771 cgcaatgatc actggctcga tggggatgtt gccttccgcc agcctgaacg agcaaggttt tggactgtat 
0841 gaaccggcgg gcggctcggc accagatatc gcaggcaaaa acatcgccaa cccgattgca caaatccttt 
0911 cgctggcact gctgctgcgt tacagcctgg atgccgatga tgcggcttgc gccattgaac gcgccattaa 
0981 ccgcgcatta gaagaaggca ttcgcaccgg ggatttagcc cgtggcgctg ccgccgttag taccgatgaa 
1051 atgggcgata tcattgcccg ctatgtagca gaaggggtgt aatcatggct aagacgttat acgaaaaatt 
1121 gttcgacgct cacgttgtgt acgaagccga aaacgaaacc ccactgttat atatcgaccg ccacctggtg 
1191 ca