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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(pgl-grxA)502
,
OCR48,49-18
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
sucA
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Related strains
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Strains Search
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Search strains by gene name:
sucA
Search strains by all related name:
sucA b0726 ECK0714 JW0715 lys
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General information (Go to Linear View: )
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Gene Name
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sucA
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Alternative name
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b0726,ECK0714,JW0715,lys
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Location, Length
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757,929 - 760,730
(
+
)
;
16.34
min
;
2802
(bp) ,
933
(aa)
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Product
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2-oxoglutarate decarboxylase, thiamin-requiring
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Operon Name
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sdhCDAB-sucABCD
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Note
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2-oxoglutarate dehydrogenase (decarboxylase component); GO_process: GO:0006099 - tricarboxylic acid cycle
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Function
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enzyme; Energy metabolism, carbon: TCA cycle
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Gene Ontology
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GO:0004591
;
oxoglutarate dehydrogenase (succinyl-transferring) activity ( sucA )
GO:0006096
;
glycolysis ( sucA )
GO:0008152
;
metabolic process ( sucA )
GO:0016491
;
oxidoreductase activity ( sucA )
GO:0016624
;
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor ( sucA )
GO:0030976
;
thiamin pyrophosphate binding ( sucA )
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PID
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1786945
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EC number
(KEGG Pathway)
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1.2.4.2
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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ODO1_ECOLI
(
P07015
;
P78225
)
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- Protein name
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2-oxoglutarate dehydrogenase E1 component
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- Synonyms
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EC 1.2.4.2, Alpha- ketoglutarate dehydrogenase
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- Gene name
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Name=sucA; OrderedLocusNames=b0726, c0803, z0880, ECs0751;
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- Comments
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FUNCTION:The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
CATALYTIC ACTIVITY:2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2).
COFACTOR:Thiamine pyrophosphate.
SUBUNIT:Homodimer.
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MMBR References
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J Bacteriol. 1997;179:4138-4142
Aerobic regulation of the sucABCD genes of Escherichia coli, which encode alpha-ketoglutarate dehydrogenase and succinyl coenzyme A synthetase: roles of ArcA, Fnr, and the upstream sdhCDAB promoter.
Park, S.-J., G. Chao, and R. P. Gunsalus.
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J Bacteriol. 1982;151(2):542-52.
Molecular cloning of four tricarboxylic acid cyclic genes of Escherichia coli.
Spencer ME, Guest JR.
(
6284701
)
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J Gen Microbiol. 1986;132(12):3239-51.
Transcript analysis of the citrate synthase and succinate dehydrogenase genes of Escherichia coli K12.
Wilde RJ, Guest JR.
(
3309132
)
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Biochem J. 1984;222(2):519-34.
Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli.
Wood D, Darlison MG, Wilde RJ, Guest JR.
(
6383359
)
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J Gen Microbiol. 1986;132(6):1753-62.
Cloning and expression of the succinyl-CoA synthetase genes of Escherichia coli K12.
Buck D, Spencer ME, Guest JR.
(
3543212
)
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Biochem J. 1984;223(2):507-17.
Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli.
Darlison MG, Guest JR.
(
6388571
)
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Eur J Biochem. 1984;141:351-359
Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12.
Darlison, M. G., M. E. Spencer, and J. R. Guest.
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| Sequences |
Amino acid
FASTA format
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0001 MQNSALKAWL DSSYLSGANQ SWIEQLYEDF LTDPDSVDAN WRSTFQQLPG TGVKPDQFHS QTREYFRRLA
0071 KDASRYSSTI SDPDTNVKQV KVLQLINAYR FRGHQHANLD PLGLWQQDKV ADLDPSFHDL TEADFQETFN
0141 VGSFASGKET MKLGELLEAL KQTYCGPIGA EYMHITSTEE KRWIQQRIES GRATFNSEEK KRFLSELTAA
0211 EGLERYLGAK FPGAKRFSLE GGDALIPMLK EMIRHAGNSG TREVVLGMAH RGRLNVLVNV LGKKPQDLFD
0281 EFAGKHKEHL GTGDVKYHMG FSSDFQTDGG LVHLALAFNP SHLEIVSPVV IGSVRARLDR LDEPSSNKVL
0351 PITIHGDAAV TGQGVVQETL NMSKARGYEV GGTVRIVINN QVGFTTSNPL DARSTPYCTD IGKMVQAPIF
0421 HVNADDPEAV AFVTRLALDF RNTFKRDVFI DLVCYRRHGH NEADEPSATQ PLMYQKIKKH PTPRKIYADK
0491 LEQEKVATLE DATEMVNLYR DALDAGDCVV AEWRPMNMHS FTWSPYLNHE WDEEYPNKVE MKRLQELAKR
0561 ISTVPEAVEM QSRVAKIYGD RQAMAAGEKL FDWGGAENLA YATLVDEGIP VRLSGEDSGR GTFFHRHAVI
0631 HNQSNGSTYT PLQHIHNGQG AFRVWDSVLS EEAVLAFEYG YATAEPRTLT IWEAQFGDFA NGAQVVIDQF
0701 ISSGEQKWGR MCGLVMLLPH GYEGQGPEHS SARLERYLQL CAEQNMQVCV PSTPAQVYHM LRRQALRGMR
0771 RPLVVMSPKS LLRHPLAVSS LEELANGTFL PAIGEIDELD PKGVKRVVMC SGKVYYDLLE QRRKNNQHDV
0841 AIVRIEQLYP FPHKAMQEVL QQFAHVKDFV WCQEEPLNQG AWYCSQHHFR EVIPFGASLR YAGRPASASP
0911 AVGYMSVHQK QQQDLVNDAL NVE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 ACGTAGACAA GAGCTCGCAA GTGAACCCCG
-070 GCACGCACAT CACTGTGCGT GGTAGTATCC ACGGCGAAGT AAGCATAAAA AAGATGCTTA AGGGATCACG
0001 atgcagaaca gcgctttgaa agcctggttg gactcttctt acctctctgg cgcaaaccag agctggatag
0071 aacagctcta tgaagacttc ttaaccgatc ctgactcggt tgacgctaac tggcgttcga cgttccagca
0141 gttacctggt acgggagtca aaccggatca attccactct caaacgcgtg aatatttccg ccgcctggcg
0211 aaagacgctt cacgttactc ttcaacgatc tccgaccctg acaccaatgt gaagcaggtt aaagtcctgc
0281 agctcattaa cgcataccgc ttccgtggtc accagcatgc gaatctcgat ccgctgggac tgtggcagca
0351 agataaagtg gccgatctgg atccgtcttt ccacgatctg accgaagcag acttccagga gaccttcaac
0421 gtcggttcat ttgccagcgg caaagaaacc atgaaactcg gcgagctgct ggaagccctc aagcaaacct
0491 actgcggccc gattggtgcc gagtatatgc acattaccag caccgaagaa aaacgctgga tccaacagcg
0561 tatcgagtct ggtcgcgcga ctttcaatag cgaagagaaa aaacgcttct taagcgaact gaccgccgct
0631 gaaggtcttg aacgttacct cggcgcaaaa ttccctggcg caaaacgctt ctcgctggaa ggcggtgacg
0701 cgttaatccc gatgcttaaa gagatgatcc gccacgctgg caacagcggc acccgcgaag tggttctcgg
0771 gatggcgcac cgtggtcgtc tgaacgtgct ggtgaacgtg ctgggtaaaa aaccgcaaga cttgttcgac
0841 gagttcgccg gtaaacataa agaacacctc ggcacgggtg acgtgaaata ccacatgggc ttctcgtctg
0911 acttccagac cgatggcggc ctggtgcacc tggcgctggc gtttaacccg tctcaccttg agattgtaag
0981 cccggtagtt atcggttctg ttcgtgcccg tctggacaga cttgatgagc cgagcagcaa caaagtgctg
1051 ccaatcacca tccacggtga cgccgcagtg accgggcagg gcgtggttca ggaaaccctg aacatgtcga
1121 aagcgcgtgg ttatgaagtt ggcggtacgg tacgtatcgt tatcaacaac caggttggtt tcaccacctc
1191 taatccgctg gatgcccgtt ctacgccgta ctgtactgat atcggtaaga tggttcaggc cccgattttc
1261 cacgttaacg cggacgatcc ggaagccgtt gcctttgtga cccgtctggc gctcgatttc cgtaacacct
1331 ttaaacgtga tgtcttcatc gacctggtgt gctaccgccg tcacggccac aacgaagccg acgagccgag
1401 cgcaacccag ccgctgatgt atcagaaaat caaaaaacat ccgacaccgc gcaaaatcta cgctgacaag
1471 ctggagcagg aaaaagtggc gacgctggaa gatgccaccg agatggttaa cctgtaccgc gatgcgctgg
1541 atgctggcga ttgcgtagtg gcagagtggc gtccgatgaa catgcactct ttcacctggt cgccgtacct
1611 caaccacgaa tgggacgaag agtacccgaa caaagttgag atgaagcgcc tgcaggagct ggcgaaacgc
1681 atcagcacgg tgccggaagc agttgaaatg cagtctcgcg ttgccaagat ttatggcgat cgccaggcga
1751 tggctgccgg tgagaaactg ttcgactggg gcggtgcgga aaacctcgct tacgccacgc tggttgatga
1821 aggcattccg gttcgcctgt cgggtgaaga ctccggtcgc ggtaccttct tccaccgcca cgcggtgatc
1891 cacaaccagt ctaacggttc cacttacacg ccgctgcaac atatccataa cgggcagggc gcgttccgtg
1961 tctgggactc cgtactgtct gaagaagcag tgctggcgtt tgaatatggt tatgccaccg cagaaccacg
2031 cactctgacc atctgggaag cgcagttcgg tgacttcgcc aacggtgcgc aggtggttat cgaccagttc
2101 atctcctctg gcgaacagaa atggggccgg atgtgtggtc tggtgatgtt gctgccgcac ggttacgaag
2171 ggcaggggcc ggagcactcc tccgcgcgtc tggaacgtta tctgcaactt tgtgctgagc aaaacatgca
2241 ggtttgcgta ccgtctaccc cggcacaggt ttaccacatg ctgcgtcgtc aggcgctgcg cgggatgcgt
2311 cgtccgctgg tcgtgatgtc gccgaaatcc ctgctgcgtc atccgctggc ggtttccagc ctcgaagaac
2381 tggcgaacgg caccttcctg ccagccatcg gtgaaatcga cgagcttgat ccgaagggcg tgaagcgcgt
2451 agtgatgtgt tctggtaagg tttattacga cctgctggaa cagcgtcgta agaacaatca acacgatgtc
2521 gccattgtgc gtatcgagca actctacccg ttcccgcata aagcgatgca ggaagtgttg cagcagtttg
2591 ctcacgtcaa ggattttgtc tggtgccagg aagagccgct caaccagggc gcatggtact gcagccagca
2661 tcatttccgt gaagtgattc cgtttggggc ttctctgcgt tatgcaggcc gcccggcctc cgcctctccg
2731 gcggtagggt atatgtccgt tcaccagaaa cagcaacaag atctggttaa tgacgcgctg aacgtcgaat
2801 aaATAAAGGA TACACAATGA GTAGCGTAGA TATTCTGGTC CCTGACCTGC CTGAATCCGT AGCCGATGCC
2871 ACCGTCGCAA CCTGGCATAA AAAACCCGGC GA
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