|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
DE(pgl-grxA)502
,
OCR48,49-12
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
sdhA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
sdhA
Search strains by all related name:
sdhA b0723 ECK0712 JW0713
|
General information (Go to Linear View: )
|
|
Gene Name
|
sdhA
|
|
Alternative name
|
b0723,ECK0712,JW0713
|
|
Location, Length
|
755,130 - 756,896
(
+
)
;
16.28
min
;
1767
(bp) ,
588
(aa)
|
|
Product
|
succinate dehydrogenase, flavoprotein subunit
|
|
Operon Name
|
sdhCDAB-sucABCD
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006099 - tricarboxylic acid cycle; GO_process: GO:0009060 - aerobic respiration
|
|
Function
|
enzyme; Energy metabolism, carbon: TCA cycle
|
|
Gene Ontology
|
GO:0000104
;
succinate dehydrogenase activity ( sdhA )
GO:0005515
;
protein binding ( sdhA )
GO:0005886
;
plasma membrane ( sdhA )
GO:0006099
;
tricarboxylic acid cycle ( sdhA )
GO:0006118
;
electron transport ( sdhA )
GO:0006810
;
transport ( sdhA )
GO:0016020
;
membrane ( sdhA )
GO:0016491
;
oxidoreductase activity ( sdhA )
GO:0016627
;
oxidoreductase activity, acting on the CH-CH group of donors ( sdhA )
GO:0050660
;
FAD binding ( sdhA )
|
|
PID
|
1786942
|
EC number
(KEGG Pathway)
|
1.3.99.1
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
sdhA
(
EG10931
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
DHSA_ECOLI
(
P10444
;
P78282
)
|
|
- Protein name
|
Succinate dehydrogenase flavoprotein subunit
|
|
- Synonyms
|
EC 1.3.99.1
|
|
- Gene name
|
Name=sdhA; OrderedLocusNames=b0723, c0801, z0877, ECs0748;
|
|
- Comments
|
FUNCTION:Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.
CATALYTIC ACTIVITY:Succinate + acceptor = fumarate + reduced acceptor.
PATHWAY:Tricarboxylic acid cycle.
SUBUNIT:Part of an enzyme complex containing four subunits:a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein.
SIMILARITY:Belongs to the FAD-dependent oxidoreductase family 2. FRD/SDH subfamily.
|
|
MMBR References
|
Mol Microbiol. 1995;15(3):473-82.
Regulation of succinate dehydrogenase (sdhCDAB) operon expression in Escherichia coli in response to carbon supply and anaerobiosis: role of ArcA and Fnr.
Park SJ, Tseng CP, Gunsalus RP.
(
7783618
)
|
J Gen Microbiol. 1986;132(12):3239-51.
Transcript analysis of the citrate synthase and succinate dehydrogenase genes of Escherichia coli K12.
Wilde RJ, Guest JR.
(
3309132
)
|
Biochem J. 1984;222(2):519-34.
Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli.
Wood D, Darlison MG, Wilde RJ, Guest JR.
(
6383359
)
|
J Bacteriol. 1991;173(5):1569-70.
E. coli map. Physical map locations of genes encoding components of the aerobic respiratory chain of Escherichia coli.
Calhoun MW, Newton G, Gennis RB.
(
1847904
)
|
J Gen Microbiol. 1977;102(1):183-94.
Suppression of the succinate requirement of lipoamide dehydrogenase mutants of Escherichia coli by mutations affecting succinate dehydrogenase activity.
Creaghan IT, Guest JR.
(
335020
)
|
Biochem J. 1984;223(2):507-17.
Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli.
Darlison MG, Guest JR.
(
6388571
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKLPVREFDA VVIGAGGAGM RAALQISQSG QTCALLSKVF PTRSHTVSAQ GGITVALGNT HEDNWEWHMY
0071 DTVKGSDYIG DQDAIEYMCK TGPEAILELE HMGLPFSRLD DGRIYQRPFG GQSKNFGGEQ AARTAAAADR
0141 TGHALLHTLY QQNLKNHTTI FSEWYALDLV KNQDGAVVGC TALCIETGEV VYFKARATVL ATGGAGRIYQ
0211 STTNAHINTG DGVGMAIRAG VPVQDMEMWQ FHPTGIAGAG VLVTEGCRGE GGYLLNKHGE RFMERYAPNA
0281 KDLAGRDVVA RSIMIEIREG RGCDGPWGPH AKLKLDHLGK EVLESRLPGI LELSRTFAHV DPVKEPIPVI
0351 PTCHYMMGGI PTKVTGQALT VNEKGEDVVV PGLFAVGEIA CVSVHGANRL GGNSLLDLVV FGRAAGLHLQ
0421 ESIAEQGALR DASESDVEAS LDRLNRWNNN RNGEDPVAIR KALQECMQHN FSVFREGDAM AKGLEQLKVI
0491 RERLKNARLD DTSSEFNTQR VECLELDNLM ETAYATAVSA NFRTESRGAH SRFDFPDRDD ENWLCHSLYL
0561 PESESMTRRS VNMEPKLRPA FPPKIRTY
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ACGTTAAACC GCTGGCTTTG CGCCTGATGC
-070 TGCAACTGGT GATTGTCGTT GCACTGGTGG TTTACGTGAT TTATGGATTC GTTGTGGTGT GGGGTGTGTG
0001 atgaaattgc cagtcagaga atttgatgca gttgtgattg gtgccggtgg cgcaggtatg cgcgcggcgc
0071 tgcaaatttc ccagagcggc cagacctgtg cgctgctctc taaagtcttc ccgacccgtt cccataccgt
0141 ttctgcgcaa ggcggcatta ccgttgcgct gggtaatacc catgaagata actgggaatg gcatatgtac
0211 gacaccgtga aagggtcgga ctatatcggt gaccaggacg cgattgaata tatgtgtaaa accgggccgg
0281 aagcgattct ggaactcgaa cacatgggcc tgccgttctc gcgtctcgat gatggtcgta tctatcaacg
0351 tccgtttggc ggtcagtcga aaaacttcgg cggcgagcag gcggcacgca ctgcggcagc agctgaccgt
0421 accggtcacg cactgttgca cacgctttat cagcagaacc tgaaaaacca caccaccatt ttctccgagt
0491 ggtatgcgct ggatctggtg aaaaaccagg atggcgcggt ggtgggttgt accgcactgt gcatcgaaac
0561 cggtgaagtg gtttatttca aagcccgcgc taccgtgctg gcgactggcg gagcagggcg tatttatcag
0631 tccaccacca acgcccacat taacaccggc gacggtgtcg gcatggctat ccgtgccggc gtaccggtgc
0701 aggatatgga aatgtggcag ttccacccga ccggcattgc cggtgcgggc gtactggtca ccgaaggttg
0771 ccgtggtgaa ggcggttatc tgctgaacaa acatggcgaa cgttttatgg agcgttatgc gccgaacgcc
0841 aaagacctgg cgggccgtga cgtggttgcg cgttccatca tgatcgaaat ccgtgaaggt cgcggctgtg
0911 atggtccgtg ggggccacac gcgaaactga aactcgatca cctgggtaaa gaagttctcg aatcccgtct
0981 gccgggtatc ctggagcttt cccgtacctt cgctcacgtc gatccggtga aagagccgat tccggttatc
1051 ccaacctgtc actacatgat gggcggtatt ccgaccaaag ttaccggtca ggcactgact gtgaatgaga
1121 aaggcgaaga tgtggttgtt ccgggactgt ttgccgttgg tgaaatcgct tgtgtatcgg tacacggcgc
1191 taaccgtctg ggcggcaact cgctgctgga cctggtggtc tttggtcgcg cggcaggtct gcatctgcaa
1261 gagtctatcg ccgagcaggg cgcactgcgc gatgccagcg agtctgatgt tgaagcgtct ctggatcgcc
1331 tgaaccgctg gaacaataat cgtaacggtg aagatccggt ggcgatccgt aaagcgctgc aagaatgtat
1401 gcagcataac ttctcggtct tccgtgaagg tgatgcgatg gcgaaagggc ttgagcagtt gaaagtgatc
1471 cgcgagcgtc tgaaaaatgc ccgtctggat gacacttcca gcgagttcaa cacccagcgc gttgagtgcc
1541 tggaactgga taacctgatg gaaacggcgt atgcaacggc tgtttctgcc aacttccgta ccgaaagccg
1611 tggcgcgcat agccgcttcg acttcccgga tcgtgatgat gaaaactggc tgtgccactc cctgtatctg
1681 ccagagtcgg aatccatgac gcgccgaagc gtcaacatgg aaccgaaact gcgcccggca ttcccgccga
1751 agattcgtac ttactaaTGC GGAGACAGGA AAATGAGACT CGAGTTTTCA ATTTATCGCT ATAACCCGGA
1821 TGTTGATGAT GCTCCGCGTA TGCAGGATTA CACCCTGGAA GCGGATG
|
