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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCR47,48-1
(D)
,
OCR47-3
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
nagE
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Related strains
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Strains Search
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Search strains by gene name:
nagE
Search strains by all related name:
nagE b0679 ECK0667 JW0665 pstN ptsN
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General information (Go to Linear View: )
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Gene Name
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nagE
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Alternative name
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b0679,ECK0667,JW0665,pstN,ptsN
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Location, Length
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703,167 - 705,113
(
+
)
;
15.16
min
;
1947
(bp) ,
648
(aa)
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Product
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fused N-acetyl glucosamine specific PTS enzyme: IIC, IIB, and IIA components
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Operon Name
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nagE
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Note
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PTS system, N-acetylglucosamine-specific enzyme IIABC; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009310 - amine catabolic process; GO_process: GO:0046349 - amino sugar biosynthetic process
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Function
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enzyme; Transport of small molecules: Amino acids, amines
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Gene Ontology
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GO:0005215
;
transporter activity ( nagE )
GO:0005215
;
transporter activity ( ptsN )
GO:0005351
;
sugar:hydrogen ion symporter activity ( nagE )
GO:0005351
;
sugar:hydrogen ion symporter activity ( ptsN )
GO:0005515
;
protein binding ( ptsN )
GO:0005737
;
cytoplasm ( ptsN )
GO:0005886
;
plasma membrane ( nagE )
GO:0006810
;
transport ( nagE )
GO:0006810
;
transport ( ptsN )
GO:0008643
;
carbohydrate transport ( nagE )
GO:0008982
;
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity ( nagE )
GO:0008982
;
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity ( ptsN )
GO:0009401
;
phosphoenolpyruvate-dependent sugar phosphotransferase system ( nagE )
GO:0009401
;
phosphoenolpyruvate-dependent sugar phosphotransferase system ( ptsN )
GO:0016020
;
membrane ( nagE )
GO:0016021
;
integral to membrane ( nagE )
GO:0016740
;
transferase activity ( nagE )
GO:0016740
;
transferase activity ( ptsN )
GO:0019866
;
organelle inner membrane ( nagE )
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PID
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1786894
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EC number
(KEGG Pathway)
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2.7.1.69
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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PTAA_ECOLI
(
P09323
)
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- Protein name
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PTS system, N-acetylglucosamine-specific IIABC component
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- Synonyms
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EIIABC-Nag, N-acetylglucosamine-permease IIABC component, Phosphotransferase enzyme II, ABC component, EC 2.7.1.69, EII-Nag
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- Gene name
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Name=nagE; Synonyms=pstN; OrderedLocusNames=b0679;
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- Comments
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FUNCTION:This is a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system. The IICD domains contain the sugar binding site and the transmembrane channel; the IIA domain contains the primary phosphorylation site (the donor is phospho-HPr); IIA transfers its phosphoryl group to the IIB domain which finally transfers it to the sugar.
CATALYTIC ACTIVITY:Protein N-phosphohistidine + sugar = protein histidine + sugar phosphate.
SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane.
SIMILARITY:Contains 1 PTS EIIA domain.
SIMILARITY:Contains 1 PTS EIIB domain.
SIMILARITY:Contains 1 PTS EIIC domain.
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Entry name(Acc.no)
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PTSN_ECOLI
(
P31222
)
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- Protein name
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Nitrogen regulatory IIA protein
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- Synonyms
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EC 2.7.1.69, Enzyme IIA-NTR, Phosphotransferase enzyme II, A component
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- Gene name
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Name=ptsN; Synonyms=rpoP; OrderedLocusNames=b3204, SF3244, S3462;
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- Comments
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FUNCTION:Seems to have a role in linking carbon and nitrogen assimilation.
CATALYTIC ACTIVITY:Protein N-phosphohistidine + sugar = protein histidine + sugar phosphate.
SUBCELLULAR LOCATION:Cytoplasmic.
SIMILARITY:BELONGS TO THE PTS IIA FAMILY.
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Entry name(Acc.no)
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Q8FD70
(
Q8FD70
)
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- Protein name
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Nitrogen regulatory IIA protein
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- Synonyms
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EC 2.7.1.69
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- Gene name
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Name=ptsN; OrderedLocusNames=c3964;
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- Comments
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SIMILARITY:Contains 1 histidine kinase domain.
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Entry name(Acc.no)
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Q8FJX5
(
Q8FJX5
)
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- Protein name
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PTS system, N-acetylglucosamine-specific IIABC component.
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- Synonyms
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- Gene name
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Name=nagE; OrderedLocusNames=c0755;
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- Comments
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SIMILARITY:Contains 1 HTH deoR-type DNA-binding domain.
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Entry name(Acc.no)
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Q8X9H9
(
Q8X9H9
)
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- Protein name
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PTS system, N-acetylglucosamine-specific enzyme IIABC.
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- Synonyms
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- Gene name
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Name=nagE; OrderedLocusNames=z0826;
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- Comments
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SIMILARITY:Contains 1 response regulatory domain.
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Entry name(Acc.no)
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Q8X9I8
(
Q8X9I8
)
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- Protein name
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Phosphotransferase system enzyme IIA, regulates N metabolism.
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- Synonyms
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- Gene name
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Name=ptsN; OrderedLocusNames=z4567;
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- Comments
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SUBCELLULAR LOCATION:Integral membrane protein (By similarity).
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MMBR References
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J Gen Microbiol. 1980;117(2):369-76.
Amino-sugar transport systems of Escherichia coli K12.
Jones-Mortimer MC, Kornberg HL.
(
6252281
)
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Mol Gen Genet. 1980;179(1):49-54.
Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin.
Lengeler J.
(
6450313
)
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Biochemistry. 1988;27(16):6054-61.
Sequence of cloned enzyme IIN-acetylglucosamine of the phosphoenolpyruvate:N-acetylglucosamine phosphotransferase system of Escherichia coli.
Peri KG, Waygood EB.
(
3056518
)
|
Biochem Cell Biol. 1990;68(1):123-37.
Cloning and characterization of the N-acetylglucosamine operon of Escherichia coli.
Peri KG, Goldie H, Waygood EB.
(
2190615
)
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Mol Gen Genet. 1987;209(3):618-20.
Organisation of the Escherichia coli chromosome between genes glnS and glnU, V.
Plumbridge J.
(
2828887
)
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Gene. 1988;62(2):197-207.
Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and for glucosamine-6-phosphate deaminase.
Rogers MJ, Ohgi T, Plumbridge J, Soll D.
(
3284790
)
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Mol Gen Genet. 1989;219(1-2):97-105.
Analysis of the nag regulon from Escherichia coli K12 and Klebsiella pneumoniae and of its regulation.
Vogler AP, Lengeler JW.
(
2693951
)
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Mol Gen Genet. 1991;230(1-2):270-6.
Comparison of the sequences of the nagE operons from Klebsiella pneumoniae and Escherichia coli K12: enhanced variability of the enzyme IIN-acetylglucosamine in regions connecting functional domains.
Vogler AP, Lengeler JW.
(
1745234
)
|
Biochem J. 1970;118(1):81-7.
An examination of the inhibitory effects of N-iodoacetylglucosamine on Escherichia coli and isolation of resistant mutants.
White RJ, Kent PW.
(
4919471
)
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| Sequences |
Amino acid
FASTA format
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0001 MNILGFFQRL GRALQLPIAV LPVAALLLRF GQPDLLNVAF IAQAGGAIFD NLALIFAIGV ASSWSKDSAG
0071 AAALAGAVGY FVLTKAMVTI NPEINMGVLA GIITGLVGGA AYNRWSDIKL PDFLSFFGGK RFVPIATGFF
0141 CLVLAAIFGY VWPPVQHAIH AGGEWIVSAG ALGSGIFGFI NRLLIPTGLH QVLNTIAWFQ IGEFTNAAGT
0211 VFHGDINRFY AGDGTAGMFM SGFFPIMMFG LPGAALAMYF AAPKERRPMV GGMLLSVAVT AFLTGVTEPL
0281 EFLFMFLAPL LYLLHALLTG ISLFVATLLG IHAGFSFSAG AIDYALMYNL PAASQNVWML LVMGVIFFAI
0351 YFVVFSLVIR MFNLKTPGRE DKEDEIVTEE ANSNTEEGLT QLATNYIAAV GGTDNLKAID ACITRLRLTV
0421 ADSARVNDTM CKRLGASGVV KLNKQTIQVI VGAKAESIGD AMKKVVARGP VAAASAEATP ATAAPVAKPQ
0491 AVPNAVSIAE LVSPITGDVV ALDQVPDEAF ASKAVGDGVA VKPTDKIVVS PAAGTIVKIF NTNHAFCLET
0561 EKGAEIVVHM GIDTVALEGK GFKRLVEEGA QVSAGQPILE MDLDYLNANA RSMISPVVCS NIDDFSGLII
0631 KAQGHIVAGQ TPLYEIKK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GATCTAACAA TCTGATTACA GAACATCGGC
-070 AGTACAATTT GCAGCAAAAT AAAAATACGG CTTGAAACGA GCCAAATAGG GTTCTCGTAG GGGGAATAAG
0001 atgaatattt taggtttttt ccagcgactc ggtagggcgt tacagctccc tatcgcggtg ctgccggtgg
0071 cggcactgtt gctgcgattc ggtcagccag atttacttaa cgttgcgttt attgcccagg cgggcggtgc
0141 gatttttgat aacctcgcat taatcttcgc catcggtgtg gcatccagct ggtcgaaaga cagcgctggt
0211 gcggcggcgc tggcgggtgc ggtaggttac tttgtgttaa ccaaagcgat ggtgaccatc aacccagaaa
0281 ttaacatggg tgtactggcg ggtatcatta ccggtctggt tggtggcgca gcctataacc gttggtccga
0351 tattaaactg ccggacttcc tgagcttctt cggcggcaaa cgctttgtgc cgattgccac cggattcttc
0421 tgcctggtgc tggcggccat ttttggttac gtctggccgc cggtacagca cgctatccat gcaggcggcg
0491 agtggatcgt ttctgcgggc gcgctgggtt ccggtatctt tggtttcatc aaccgtctgc tgatcccaac
0561 cggtctgcat caggtactga acaccatcgc ctggttccag attggtgaat tcaccaacgc ggcgggtacg
0631 gttttccacg gtgacattaa ccgcttctat gccggtgacg gcaccgcggg gatgttcatg tccggcttct
0701 tcccgatcat gatgttcggt ctgccgggtg cggcgctggc gatgtacttc gcagcaccga aagagcgtcg
0771 tccgatggtt ggcggtatgc tgctttctgt tgctgttact gcgttcctga ccggtgtgac tgagccgctg
0841 gaattcctgt tcatgttcct tgctccgctg ctgtacctcc tgcacgcact gctgaccggt atcagcctgt
0911 ttgtggcaac gctgctgggt atccacgcgg gcttctcttt ctctgcgggg gctatcgact acgcgttgat
0981 gtataacctg ccggccgcca gccagaacgt ctggatgctg ctggtgatgg gcgttatctt cttcgctatc
1051 tacttcgtgg tgttcagttt ggttatccgc atgttcaacc tgaaaacgcc gggtcgtgaa gataaagaag
1121 acgagatcgt tactgaagaa gccaacagca acactgaaga aggtctgact caactggcaa ccaactatat
1191 tgctgcggtt ggcggcactg acaacctgaa agcgattgac gcctgtatca cccgtctgcg ccttacagtg
1261 gctgactctg cccgcgttaa cgatacgatg tgtaaacgtc tgggtgcttc tggggtagtg aaactgaaca
1331 aacagactat tcaggtgatt gttggcgcga aagcagaatc catcggcgat gcgatgaaga aagtcgttgc
1401 ccgtggtccg gtagccgctg cgtcagctga agcaactccg gcaactgccg cgcctgtagc aaaaccgcag
1471 gctgtaccaa acgcggtatc tatcgcggag ctggtatcgc cgattaccgg tgatgtcgtg gcactggatc
1541 aggttcctga cgaagcattc gccagcaaag cggtgggtga cggtgtggcg gtgaaaccga cagataaaat
1611 cgtcgtatca ccagccgcag ggacaatcgt gaaaatcttc aacaccaacc acgcgttctg cctggaaacc
1681 gaaaaaggcg cggagatcgt cgtccatatg ggtatcgaca ccgtagcgct ggaaggtaaa ggctttaaac
1751 gtctggtgga agagggtgcg caggtaagcg cagggcaacc gattctggaa atggatctgg attacctgaa
1821 cgctaacgcc cgctcgatga ttagcccggt ggtttgcagc aatatcgacg atttcagtgg cttgatcatt
1891 aaagctcagg gccatattgt ggcgggtcaa acaccgctgt atgaaatcaa aaagtaaTCT GCTTTATGCC
1961 TGATGCGACG CTTGAGCGTC GCATCCAACA ATGACAAGCG GTGGAGATCT TCTCTGCCGC TTTTTTTTTC
2031 ATCAATCATC CCCATAA
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