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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
DNA Cell Biol. 1990;9(9):631-5.
Nucleotide sequence and deletion analysis of the polB gene of Escherichia coli.
Chen H, Sun Y, Stark T, Beattie W, Moses RE.
(
2261080
)
|
|
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Deletion
|
OCR29
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
polB
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Related strains
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Strains Search
|
Search strains by gene name:
polB
Search strains by all related name:
polB b0060 dinA ECK0061 JW0059
|
General information (Go to Linear View: )
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Gene Name
|
polB
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Alternative name
|
b0060,dinA,ECK0061,JW0059
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Location, Length
|
63,429 - 65,780
(
-
)
;
1.37
min
;
2352
(bp) ,
783
(aa)
|
|
Product
|
DNA polymerase II
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Operon Name
|
polB
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication; GO_process: GO:0006281 - DNA repair; GO_process: GO:0009314 - response to radiation; GO_process: GO:0009432 - SOS response
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( polB )
GO:0003676
;
nucleic acid binding ( polB )
GO:0003677
;
DNA binding ( polB )
GO:0003887
;
DNA-directed DNA polymerase activity ( polB )
GO:0005515
;
protein binding ( polB )
GO:0006139
;
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process ( polB )
GO:0006260
;
DNA replication ( polB )
GO:0006281
;
DNA repair ( polB )
GO:0006974
;
response to DNA damage stimulus ( polB )
GO:0009432
;
SOS response ( polB )
GO:0016740
;
transferase activity ( polB )
GO:0016779
;
nucleotidyltransferase activity ( polB )
|
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PID
|
1786246
|
EC number
(KEGG Pathway)
|
2.7.7.7
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
polB
(
EG10747
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
DPO2_ECOLI
(
P21189
;
Q8KMX7; Q8KMX8
)
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- Protein name
|
DNA polymerase II
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- Synonyms
|
EC 2.7.7.7, Pol II
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- Gene name
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Name=polB; Synonyms=dinA; OrderedLocusNames=b0060;
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- Comments
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FUNCTION:Thought to be involved in DNA repair and/or mutagenesis.
CATALYTIC ACTIVITY:N deoxynucleoside triphosphate = N diphosphate + {DNA}(N).
ENZYME REGULATION:DNA polymerase II activity is regulated by the lexA gene during the SOS response.
SIMILARITY:Belongs to the DNA polymerase type-B family.
CAUTION:Ref.3 sequence differs from that shown due to a frameshift in position 238.
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Entry name(Acc.no)
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Q8FL91
(
Q8FL91
)
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- Protein name
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DNA polymerase II
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- Synonyms
|
EC 2.7.7.7
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- Gene name
|
Name=polB; OrderedLocusNames=c0071;
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- Comments
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CATALYTIC ACTIVITY:N deoxynucleoside triphosphate = N diphosphate + {DNA}(N).
SIMILARITY:Belongs to the DNA polymerase type-B family.
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Entry name(Acc.no)
|
Q8XA09
(
Q8XA09
)
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- Protein name
|
DNA polymerase II.
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- Synonyms
|
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- Gene name
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Name=polB; OrderedLocusNames=z0068;
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- Comments
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CATALYTIC ACTIVITY:N deoxynucleoside triphosphate = N diphosphate + {DNA}(N).
SIMILARITY:Belongs to the DNA polymerase type-B family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
polB
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain A, Crystal Structure Of Escherichia Coli Dna Polymerase Ii
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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DNA polymerase II
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PSI-BLAST
|
DNA polymerase II
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nr
(database updated :
2007.02.20
)
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BLAST
|
DNA polymerase II [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
DNA_POLYMERASE_B
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MMBR References
|
J Bacteriol. 1994;176:6221-6228
Involvement of Escherichia coli DNA polymerase II in response to oxidative damage and adaptive mutation.
Escarceller, M., J. Hicks, G. Gudmundsson, G. Trump, D. Touati, S. T. Lovett, P. L. Foster, K. McEntee, and M. F. Goodman.
|
Mol Gen Genet. 1991;226(1-2):24-33.
Escherichia coli DNA polymerase II is homologous to alpha-like DNA polymerases.
Iwasaki H, Ishino Y, Toh H, Nakata A, Shinagawa H.
(
2034216
)
|
J Bacteriol. 1992;174:3377-3385
Isolation of DNA damage-inducible promoters in Escherichia coli: regulation of polB (dinA), dinG, and dinH by LexA repressor.
Lewis, L. K., M. E. Jenkins, and D. W. Mount.
|
J Biol Chem. 1996;271:24662-24669
Mechanism of translesion DNA synthesis by DNA polymerase II. Comparison to DNA polymerases I and III core.
Paz-Elizur, T., M. Takeshita, M. Goodman, M. O'Donnell, and Z. Livneh.
|
Biochimie. 1991;73(4):433-5.
SOS-inducible DNA polymerase II of E coli is homologous to replicative DNA polymerase of eukaryotes.
Shinagawa H, Iwasaki H, Ishino Y, Nakata A.
(
1911943
)
|
Proc Natl Acad Sci U S A. 1990;87:7663-7667
DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli.
Bonner, C. A., S. Hays, K. McEntee, and M. F. Goodman.
|
J Biol Chem. 1989;264:20591-20595
Cloning the polB gene of Escherichia coli and identification of its product.
Chen, H., S. K. Bryan, and R. E. Moses.
|
DNA Cell Biol. 1990;9(9):631-5.
Nucleotide sequence and deletion analysis of the polB gene of Escherichia coli.
Chen H, Sun Y, Stark TF, Beattie WG, Moses RE.
(
2261080
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MAQAGFILTR HWRDTPQGTE VSFWLATDNG PLQVTLAPQE SVAFIPADQV PRAQHILQGE QGFRLTPLAL
0071 KDFHRQPVYG LYCRAHRQLM NYEKRLREGG VTVYEADVRP PERYLMERFI TSPVWVEGDM HNGTIVNARL
0141 KPHPDYRPPL KWVSIDIETT RHGELYCIGL EGCGQRIVYM LGPENGDASS LDFELEYVAS RPQLLEKLNA
0211 WFANYDPDVI IGWNVVQFDL RMLQKHAERY RLPLRLGRDN SELEWREHGF KNGVFFAQAK GRLIIDGIEA
0281 LKSAFWNFSS FSLETVAQEL LGEGKSIDNP WDRMDEIDRR FAEDKPALAT YNLKDCELVT QIFHKTEIMP
0351 FLLERATVNG LPVDRHGGSV AAFGHLYFPR MHRAGYVAPN LGEVPPHASP GGYVMDSRPG LYDSVLVLDY
0421 KSLYPSIIRT FLIDPVGLVE GMAQPDPEHS TEGFLDAWFS REKHCLPEIV TNIWHGRDEA KRQGNKPLSQ
0491 ALKIIMNAFY GVLGTTACRF FDPRLASSIT MRGHQIMRQT KALIEAQGYD VIYGDTDSTF VWLKGAHSEE
0561 EAAKIGRALV QHVNAWWAET LQKQRLTSAL ELEYETHFCR FLMPTIRGAD TGSKKRYAGL IQEGDKQRMV
0631 FKGLETVRTD WTPLAQQFQQ ELYLRIFRNE PYQEYVRETI DKLMAGELDA RLVYRKRLRR PLSEYQRNVP
0701 PHVRAARLAD EENQKRGRPL QYQNRGTIKY VWTTNGPEPL DYQRSPLDYE HYLTRQLQPV AEGILPFIED
0771 NFATLMTGQL GLF
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gcgcgaaggc atattacggg cagtaatgac
-070 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg gattttcagc
0001 gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca agggacagaa gtctccttct
0071 ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc accgcaagag tccgtggcgt ttattcccgc
0141 cgatcaggtt ccccgcgctc agcatatttt gcagggtgaa caaggctttc gcctgacacc gctggcgtta
0211 aaggattttc accgccagcc ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa
0281 agcgcctgcg tgaaggtggc gttaccgtct acgaggccga tgtgcgtccg ccagaacgct atctgatgga
0351 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa tgcccgtctg
0421 aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat tgaaaccacc cgccacggtg
0491 agctgtactg catcggcctg gaaggctgcg ggcagcgcat cgtttatatg ctggggccgg agaatggcga
0561 cgcctcctcg cttgatttcg aactggaata cgtcgccagc cgcccgcagt tgctggaaaa actcaacgcc
0631 tggtttgcca actacgatcc tgatgtgatc atcggttgga acgtggtgca gttcgatctg cgaatgctgc
0701 aaaaacatgc cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
0771 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg tatcgaggcg
0841 ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc tcaggagcta ttaggcgaag
0911 gaaaatctat cgataacccg tgggatcgaa tggacgaaat tgaccgccgt ttcgccgaag ataaacctgc
0981 gctggcaact tataacctga aagattgcga gctggtgacg cagatcttcc acaaaactga aatcatgcca
1051 tttttactcg aacgggcaac ggtgaacggc ctgccggtgg accgacacgg cggttcggtg gcggcatttg
1121 gtcatctcta ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
1191 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt gctggactat
1261 aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg gctggtggaa ggcatggcgc
1331 agcctgatcc agagcacagt accgaaggtt ttctcgatgc ctggttctcg cgagaaaaac attgcctgcc
1401 ggagattgtg actaacatct ggcacgggcg cgatgaagcc aaacgccagg gtaacaaacc gctgtcgcag
1471 gcgctgaaaa tcatcatgaa tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc
1541 ggctggcatc gtcgatcacc atgcgtggtc atcagatcat gcggcaaacc aaagcgttga ttgaagcaca
1611 gggctacgac gttatctacg gcgataccga ctcaacgttt gtctggctga aaggcgcaca ttcggaagaa
1681 gaagcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg ggcggaaacg ctgcaaaaac
1751 aacggctgac cagcgcatta gaactggagt atgaaaccca tttctgccgt tttctgatgc caaccattcg
1821 cggagccgat accggcagta aaaagcgtta tgccggactg attcaggagg gcgacaagca gcggatggtg
1891 tttaaagggc tggaaaccgt gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctatacc
1961 tgcgcatctt ccgcaacgag ccatatcagg aatatgtacg cgaaaccatc gacaaactga tggcgggtga
2031 actggatgcg cgactggttt accgtaaacg ccttcgccgt ccgctgagcg agtatcagcg taatgtgccg
2101 cctcatgtac gcgccgctcg ccttgccgat gaagaaaacc aaaagcgtgg tcgccccttg caatatcaga
2171 atcgcggcac cattaagtac gtatggacca ccaacggccc ggagccgctg gactaccaac gttcaccact
2241 ggattacgaa cactatctga cccgccagct acaacccgtg gcggagggaa tactcccttt tattgaggat
2311 aattttgcta cacttatgac cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat
2381 tttgatgcgt gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
2451 ca
|
