MG1655
W3110
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Gene Report : lptD
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Mol Microbiol. 2002;45(5):1289-302.
Imp/OstA is required for cell envelope biogenesis in Escherichia coli.
Braun M, Silhavy TJ. ( 12207697 )
     Deletion OCR29 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  lptD
Related strains
     Strains Search Search strains by gene name:  lptD   Search strains by all related name:  lptD b0054 ECK0055 imp JW0053 ostA yabG

General information  (Go to Linear View:)
 Gene Name lptD  
 Alternative name b0054,ECK0055,imp,JW0053,ostA,yabG  
 Location, Length 54,755 - 57,109 (  -  ) ;   1.18 min ; 2355 (bp) ,   784 (aa) Go to Linear View
 Product LPS assembly OM complex LptDE, beta-barrel component  
 Operon Name lptD-surA-pdxA-rsmA-apaGH  
 Note organic solvent tolerance; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane  
 Function phenotype; Adaptations, atypical conditions  
 Gene Ontology GO:0009279 ; cell outer membrane ( imp )
GO:0010033 ; response to organic substance ( imp )
GO:0016020 ; membrane ( imp )
GO:0016044 ; membrane organization and biogenesis ( imp )
GO:0019867 ; outer membrane ( imp )
GO:0043165 ; outer membrane biogenesis (sensu Proteobacteria) ( imp )
 PID 1786239  
 EC number
  (KEGG Pathway)   		 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene imp ( EG11569 )  
    Number of removed 24 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) OSTA_ECOLI ( P31554 ; P75631 )
    -  Protein name Organic solvent tolerance protein precursor.  
    -  Synonyms  
    -  Gene name Name=imp; Synonyms=ostA; OrderedLocusNames=b0054;  
    -  Comments
  • FUNCTION:Determines N-hexane tolerance. Involved in outer membrane permeability. Essential for envelope biogenesis. Could be part of a targeting/usher system for outer membrane components.
  • SUBUNIT:Forms a high-molecular-weight complex in the outer membrane.
  • SUBCELLULAR LOCATION:Outer membrane.
  • PTM:Forms at least two disulfide briges, one inter- and one intramolecular.
  • SIMILARITY:Belongs to the imp/ostA family.  
    •   botton Entry name(Acc.no) OSTA_ECOL6 ( Q8CWE6 )
        -  Protein name Organic solvent tolerance protein precursor.  
        -  Synonyms  
        -  Gene name Name=imp; Synonyms=ostA; OrderedLocusNames=c0067;  
        -  Comments
  • FUNCTION:Determines N-hexane tolerance. Involved in outer membrane permeability. Essential for envelope biogenesis. Could be part of a targeting/usher system for outer membrane components (By similarity).
  • SUBCELLULAR LOCATION:Outer membrane (By similarity).
  • SIMILARITY:Belongs to the imp/ostA family.  
    •   botton Entry name(Acc.no) OSTA_ECO57 ( Q8XA13 )
        -  Protein name Organic solvent tolerance protein precursor.  
        -  Synonyms  
        -  Gene name Name=imp; Synonyms=ostA; OrderedLocusNames=z0063, ECs0059;  
        -  Comments
  • FUNCTION:Determines N-hexane tolerance. Involved in outer membrane permeability. Essential for envelope biogenesis. Could be part of a targeting/usher system for outer membrane components (By similarity).
  • SUBUNIT:Forms a high-molecular-weight complex in the outer membrane (By similarity).
  • SUBCELLULAR LOCATION:Outer membrane (By similarity).
  • PTM:Forms at least two disulfide briges, one inter- and one intramolecular (By similarity).
  • SIMILARITY:Belongs to the imp/ostA family.  

    •  Linear View (Whole Mode)
    View Location
       45.0  –  70.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP imp (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Structure At 1.9 A Resolution Of A Quinohemoprotein Alcohol Dehydrogenase From Pseudomonas Putida Hk5  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Organic solvent tolerance protein precursor  
             PSI-BLAST Organic solvent tolerance protein precursor
        nr     (database updated : 2007.02.20 )
             BLAST organic solvent tolerance protein precursor [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

    Other Cross-Reference
        COG COG1452M 
        EcoCyc lptD 

    MMBR References
    Genetics 1989;122:491-501
    Identification and characterization of a new gene of Escherichia coli K-12 involved in outer membrane permeability.
    Sampson, B. A., R. Misra, and S. A. Benson.

    Sequences
    Amino acid
    FASTA format 
    0001 MKKRIPTLLA TMIATALYSQ QGLAADLASQ CMLGVPSYDR PLVQGDTNDL PVTINADHAK GDYPDDAVFT 
0071 GSVDIMQGNS RLQADEVQLH QKEAPGQPEP VRTVDALGNV HYDDNQVILK GPKGWANLNT KDTNVWEGDY 
0141 QMVGRQGRGK ADLMKQRGEN RYTILDNGSF TSCLPGSDTW SVVGSEIIHD REEQVAEIWN ARFKVGPVPI 
0211 FYSPYLQLPV GDKRRSGFLI PNAKYTTTNY FEFYLPYYWN IAPNMDATIT PHYMHRRGNI MWENEFRYLS 
0281 QAGAGLMELD YLPSDKVYED EHPNDDSSRR WLFYWNHSGV MDQVWRFNVD YTKVSDPSYF NDFDNKYGSS 
0351 TDGYATQKFS VGYAVQNFNA TVSTKQFQVF SEQNTSSYSA EPQLDVNYYQ NDVGPFDTRI YGQAVHFVNT 
0421 RDDMPEATRV HLEPTINLPL SNNWGSINTE AKLLATHYQQ TNLDWYNSRN TTKLDESVNR VMPQFKVDGK 
0491 MVFERDMEML APGYTQTLEP RAQYLYVPYR DQSDIYNYDS SLLQSDYSGL FRDRTYGGLD RIASANQVTT 
0561 GVTSRIYDDA AVERFNISVG QIYYFTESRT GDDNITWEND DKTGSLVWAG DTYWRISERW GLRGGIQYDT 
0631 RLDNVATSNS SIEYRRDEDR LVQLNYRYAS PEYIQATLPK YYSTAEQYKN GISQVGAVAS WPIADRWSIV 
0701 GAYYYDTNAN KQADSMLGVQ YSSCCYAIRV GYERKLNGWD NDKQHAVYDN AIGFNIELRG LSSNYGLGTQ 
0771 EMLRSNILPY QNTL
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             ttcggacagg attaacacta gcgtagagat 
-070 gacgagtacg ttagtctctg accgtttgtc acgcgcaacg ttaccgatga tggaacaata aaatcaacgt 
0001 atgaaaaaac gtatccccac tctcctggcc accatgattg ccaccgccct ttatagtcaa cagggactgg 
0071 cagccgacct cgcctcacag tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac 
0141 caatgactta cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact 
0211 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat caaaaagagg 
0281 caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc cattacgacg ataaccaggt 
0351 gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc aaagatacca acgtctggga aggtgattac 
0421 cagatggtgg gtcgccaggg tcgcggtaaa gcggacctga tgaaacaacg tggcgaaaac cgctatacca 
0491 ttctggataa cggtagcttt acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat 
0561 tattcatgac cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc 
0631 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc ccgaacgcca 
0701 agtacaccac caccaactac tttgagttct acctgccata ttactggaac atcgcgccaa atatggatgc 
0771 caccatcacg ccgcattata tgcatcgtcg tggcaacatc atgtgggaga acgaattccg ctacctctcc 
0841 caggcgggcg ctggcttgat ggaactggac tatctgcctt cagataaagt ctatgaagat gaacacccga 
0911 acgatgacag ttcacgtcgt tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt 
0981 caacgtcgac tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt 
1051 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc accgtttcaa 
1121 ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca gagccgcagt tagacgttaa 
1191 ttactaccag aatgatgttg gtccgtttga tacgcgtatt tacggccagg cagtgcactt tgttaacacc 
1261 agagacgaca tgcctgaagc aacccgtgtt cacctggaac cgaccatcaa tttgccgctc tctaataact 
1331 ggggcagcat caataccgaa gcgaagttgc tggcaaccca ttatcagcaa accaatcttg actggtataa 
1401 ctccagaaac acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa 
1471 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg cgcgcgcagt 
1541 atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg tctctgctgc aatctgacta 
1611 ctctggcctg ttccgggacc ggacttacgg cggtcttgac cgtattgcct ccgctaacca ggtgacgacc 
1681 ggtgtcacat ctcgcatata tgatgatgct gccgttgaac gttttaatat ttccgttggt caaatctact 
1751 atttcacgga gtctcgcact ggcgatgaca acataacatg ggagaatgac gacaaaacgg gttcactggt 
1821 gtgggcaggc gatacttact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca 
1891 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt ctggtacagc 
1961 tgaattaccg ttacgccagc ccggaatata ttcaggctac gctgcctaag tactattcca ctgctgagca 
2031 atataagaat ggtatttcgc aggtaggtgc tgtcgccagc tggccaattg ccgatcgttg gtccattgtt 
2101 ggggcctact actacgacac caatgctaac aagcaagccg actctatgtt aggtgtgcaa tacagctcct 
2171 gctgctatgc aattcgcgtc ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt 
2241 atatgacaac gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa 
2311 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca cgtaatccgc 
2381 agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct tctcggtatc gccatgatcg 
2451 cgaat