|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Deletion
|
DE(deoD-arcA)253
,
OCR26,27-4
(D)
,
OCR26,27-5
(D)
,
OCR27-7
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
nadR
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
nadR
Search strains by all related name:
nadR b4390 ECK4382 JW5800 nadI o417b pnuA
|
General information (Go to Linear View: )
|
|
Gene Name
|
nadR
|
|
Alternative name
|
b4390,ECK4382,JW5800,nadI,o417b,pnuA
|
|
Location, Length
|
4,625,338 - 4,626,570
(
+
)
;
99.69
min
;
1233
(bp) ,
410
(aa)
|
|
Product
|
trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
|
|
Operon Name
|
serB-radA-nadR
|
|
Note
|
probable nadAB transcriptional regulator; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0009435 - NAD biosynthetic process; GO_process: GO:0006350 - transcription
|
|
Function
|
regulator; Biosynthesis of cofactors, carriers: Pyridine nucleotide
|
|
Gene Ontology
|
GO:0003677
;
DNA binding ( nadR )
GO:0003824
;
catalytic activity ( nadR )
GO:0005737
;
cytoplasm ( nadR )
GO:0006350
;
transcription ( nadR )
GO:0006355
;
regulation of transcription, DNA-dependent ( nadR )
GO:0006810
;
transport ( nadR )
GO:0009058
;
biosynthetic process ( nadR )
GO:0016020
;
membrane ( nadR )
GO:0019363
;
pyridine nucleotide biosynthetic process ( nadR )
GO:0030528
;
transcription regulator activity ( nadR )
GO:0043565
;
sequence-specific DNA binding ( nadR )
GO:0045449
;
regulation of transcription ( nadR )
|
|
PID
|
87082440
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
NADR_ECOLI
(
P27278
;
P76819
)
|
|
- Protein name
|
Transcriptional regulator nadR.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=nadR; Synonyms=nadI; OrderedLocusNames=b4390;
|
|
- Comments
|
FUNCTION:NadR is bifunctional; it interacts with pnuC at low internal NAD levels, permitting transport of NMN intact into the cell. As NAD levels increase within the cell, the affinity of nadR for the operator regions of nadA, nadB, and pncB increases, repressing the transcription of these genes.
SUBCELLULAR LOCATION:May loosely associate with the membrane through its interaction with the membrane-bound pnuC. In combination with corepressor (NAD?), the cytoplasmic form of nadR would be capable of acting as a transcriptional repressor.
SIMILARITY:Contains 1 HTH cro/C1-type DNA-binding domain.
|
|
Entry name(Acc.no)
|
Q8FA46
(
Q8FA46
)
|
|
- Protein name
|
Transcriptional regulator nadR.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=nadR; OrderedLocusNames=c5475;
|
|
- Comments
|
SIMILARITY:Belongs to the ABC transporter family.
|
|
Entry name(Acc.no)
|
Q8XB21
(
Q8XB21
)
|
|
- Protein name
|
Probable nadAB transcriptional regulator.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=nadR; OrderedLocusNames=z5991;
|
|
- Comments
|
SIMILARITY:Contains 1 response regulatory domain.
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
nadR
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Nadr Protein From Haemophilus Influenzae
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Transcriptional regulator nadR
|
|
PSI-BLAST
|
Lipid A export ATP-binding/permease protein msbA
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
transcription regulator nadR - Escherichia coli (strain K-12)
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
ATP_GTP_A
ALDEHYDE_DEHYDR_GLU
|
| Sequences |
Amino acid
FASTA format
|
0001 MSSFDYLKTA IKQQGCTLQQ VADASGMTKG YLSQLLNAKI KSPSAQKLEA LHRFLGLEFP RQKKTIGVVF
0071 GKFYPLHTGH IYLIQRACSQ VDELHIIMGF DDTRDRALFE DSAMSQQPTV PDRLRWLLQT FKYQKNIRIH
0141 AFNEEGMEPY PHGWDVWSNG IKKFMAEKGI QPDLIYTSEE ADAPQYMEHL GIETVLVDPK RTFMSISGAQ
0211 IRENPFRYWE YIPTEVKPFF VRTVAILGGE SSGKSTLVNK LANIFNTTSA WEYGRDYVFS HLGGDEIALQ
0281 YSDYDKIALG HAQYIDFAVK YANKVAFIDT DFVTTQAFCK KYEGREHPFV QALIDEYRFD LVILLENNTP
0351 WVADGLRSLG SSVDRKEFQN LLVEMLEENN IEFVRVEEED YDSRFLRCVE LVREMMGEQR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGAAAAAAGC GCCGGAAGGG ATGCAGATTT
-070 TTGGCGTTAA AAAACTCTCC GACGCGCTTA GCGTGTTCGA CGACTTATAA TGAGATATAC GGAGGGAGAT
0001 atgtcgtcat ttgattacct gaaaactgcc atcaagcaac agggctgcac gctacagcag gtagctgatg
0071 ccagcggtat gaccaaaggg tatttaagcc agttactgaa tgccaaaatc aaaagcccca gcgcgcaaaa
0141 gctggaggcg ttgcaccgtt ttttggggct tgagtttccc cggcagaaga aaacgatcgg tgtcgtattc
0211 ggtaagttct acccactgca taccggacat atctacctta tccagcgcgc ctgtagccag gttgacgagc
0281 tgcatatcat tatgggtttt gacgataccc gtgaccgcgc gttgttcgaa gacagtgcca tgtcgcagca
0351 gccgaccgtg ccggatcgtc tgcgttggtt attgcaaact tttaaatatc agaaaaatat tcgcattcat
0421 gctttcaacg aagagggcat ggagccgtat ccgcacggct gggatgtgtg gagcaacggc atcaaaaagt
0491 ttatggctga aaaagggatc cagccggatc tgatctacac ctcggaagaa gccgatgcgc cacagtatat
0561 ggaacatctg gggatcgaga cggtgctggt cgatccgaaa cgtaccttta tgagtatcag cggtgcgcag
0631 atccgcgaaa acccgttccg ctactgggaa tatattccta ccgaagtgaa gccgtttttt gtgcgtaccg
0701 tggcgatcct tggcggcgag tcgagcggta aatccaccct ggtaaacaaa cttgccaata tcttcaacac
0771 caccagtgcg tgggaatatg gccgcgatta tgtcttttca cacctcggcg gtgatgagat cgcattgcag
0841 tattctgact acgataaaat cgcgctgggc cacgctcaat acattgattt tgcggtgaaa tatgccaata
0911 aagtggcatt tatcgatacc gattttgtca ccactcaggc gttctgcaaa aagtacgaag ggcgggaaca
0981 tccgttcgtg caggcgctga ttgatgaata ccgtttcgat ctggtgatcc tgctggagaa caacacgccg
1051 tgggtggcgg atggtttacg cagcctcggc agttcggtgg atcgcaaaga gttccagaac ttgctggtgg
1121 agatgctcga agagaacaat atcgaatttg tgcgggttga agaggaagat tacgacagtc gtttcctgcg
1191 ctgcgtggaa ctggtgcggg agatgatggg ggagcagaga taaCCGTGAT GAAACTGCTC AAAGGCGAGG
1261 TATAAAATGA GTTTTTTTGA TGAGTTGAAA ACCTCTCTGG AAGAGGCTGT CGAGATTAAA CAAGGTTTGA
1331 AAA
|
