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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Genetika. 1975;11(4):97-105.
[A further study of the nature of phenotypical reversions in thymidine phosphorylase deletion mutants of Escherichia coli K-12]
Mironov AS, Smirnov IuV, Sukhodolets VV.
(
776741
)
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Deletion
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OCR26
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
deoA
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Related strains
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Strains Search
|
Search strains by gene name:
deoA
Search strains by all related name:
deoA b4382 ECK4374 JW4345 TP tpp tpp-75 ttg
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General information (Go to Linear View: )
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Gene Name
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deoA
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Alternative name
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b4382,ECK4374,JW4345,TP,tpp,tpp-75,ttg
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Location, Length
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4,616,252 - 4,617,574
(
+
)
;
99.50
min
;
1323
(bp) ,
440
(aa)
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Product
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thymidine phosphorylase
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Operon Name
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deoCABD
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Note
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GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion
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Function
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enzyme; Salvage of nucleosides and nucleotides
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Gene Ontology
|
GO:0005515
;
protein binding ( deoA )
GO:0005624
;
membrane fraction ( deoA )
GO:0006206
;
pyrimidine base metabolic process ( deoA )
GO:0006213
;
pyrimidine nucleoside metabolic process ( deoA )
GO:0008152
;
metabolic process ( deoA )
GO:0009032
;
thymidine phosphorylase activity ( deoA )
GO:0016740
;
transferase activity ( deoA )
GO:0016757
;
transferase activity, transferring glycosyl groups ( deoA )
GO:0016763
;
transferase activity, transferring pentosyl groups ( deoA )
GO:0046104
;
thymidine metabolic process ( deoA )
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PID
|
1790842
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EC number
(KEGG Pathway)
|
2.4.2.4
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SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
TYPH_ECOLI
(
P07650
)
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- Protein name
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Thymidine phosphorylase
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- Synonyms
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EC 2.4.2.4, TdRPase
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- Gene name
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Name=deoA; Synonyms=tpp, ttg; OrderedLocusNames=b4382;
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- Comments
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FUNCTION:The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis.
CATALYTIC ACTIVITY:Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.
PATHWAY:Nucleotide and deoxyribonucleotide catabolism.
SUBUNIT:Homodimer.
SIMILARITY:Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
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Entry name(Acc.no)
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Q8FA52
(
Q8FA52
)
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- Protein name
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Thymidine phosphorylase
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- Synonyms
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EC 2.4.2.4
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- Gene name
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Name=deoA; OrderedLocusNames=c5466;
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- Comments
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FUNCTION:May play a role in the repair of endogenous alkylation damage (By similarity).
SIMILARITY:Belongs to the recA family.
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Entry name(Acc.no)
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Q8XB35
(
Q8XB35
)
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- Protein name
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Thymidine phosphorylase.
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- Synonyms
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- Gene name
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Name=deoA; OrderedLocusNames=z5984;
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- Comments
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FUNCTION:May play a role in the repair of endogenous alkylation damage (By similarity).
SIMILARITY:Belongs to the recA family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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deoA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain , Thymidine Phosphorylase (E.C.2.4.2.4)
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Thymidine phosphorylase
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PSI-BLAST
|
Thymidine phosphorylase
|
|
nr
(database updated :
2007.02.20
)
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BLAST
|
thymidine phosphorylase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
|
GLYCOSAMINOGLYCAN
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
PROKAR_LIPOPROTEIN
THYMID_PHOSPHORYLASE
|
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MMBR References
|
Gene. 1982;17(3):291-8.
The cloning of the Escherichia coli K-12 deoxyribonucleoside operon.
Fischer M, Short SA.
(
6286410
)
|
Mol Gen Genet. 1969;104(4):351-9.
A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxynucleosides in Escherichia coli.
Ahmad SI, Pritchard RH.
(
4904508
)
|
Genetika. 1996;32(1):42-52.
[Unequal crossing over in Escherichia coli: genetic and physical mapping of duplications isolated in conjugational matings]
Sukhodolets VV, Dukhii DE.
(
8647423
)
|
EMBO J. 1982;1:317-322.
The structure of tandem regulatory regions in the deo operon of Escherichia coli K12.
Valentin-Hansen, P., H. Aiba, and D. Schumperli.
|
Nucleic Acids Res. 1984;12:5211-5224
The internal regulated promoter of the deo operon of Escherichia coli K-12.
Valentin-Hansen, P., K. Hammer, E. L. Larsen, and I. Svendsen.
|
EMBO J. 1984;3:179-183
Structure and function of the intercistronic regulatory deoC-deoA element of Escherichia coli K-12.
Valentin-Hansen, P., K. Hammer-Jespersen, F. Boetius, and I. Svendsen.
|
J Mol Biol. 1979;133(1):1-17.
Evidence for the existence of three promoters for the deo operon of Escherichia coli K12 in vitro.
Valentin-Hansen P, Hammer-Jespersen K, Buxton RS.
(
231107
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MFLAQEIIRK KRDGHALSDE EIRFFINGIR DNTISEGQIA ALAMTIFFHD MTMPERVSLT MAMRDSGTVL
0071 DWKSLHLNGP IVDKHSTGGV GDVTSLMLGP MVAACGGYIP MISGRGLGHT GGTLDKLESI PGFDIFPDDN
0141 RFREIIKDVG VAIIGQTSSL APADKRFYAT RDITATVDSI PLITASILAK KLAEGLDALV MDVKVGSGAF
0211 MPTYELSEAL AEAIVGVANG AGVRTTALLT DMNQVLASSA GNAVEVREAV QFLTGEYRNP RLFDVTMALC
0281 VEMLISGKLA KDDAEARAKL QAVLDNGKAA EVFGRMVAAQ KGPTDFVENY AKYLPTAMLT KAVYADTEGF
0351 VSEMDTRALG MAVVAMGGGR RQASDTIDYS VGFTDMARLG DQVDGQRPLA VIHAKDENNW QEAAKAVKAA
0421 IKLADKAPES TPTVYRRISE
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCGCTTATCA GGCCTACGAG ACGTATCTAC
-070 CCGTAGGCCG GATAAGGCGT AGACGCATCC GGCAAAAGCC GCCTCATACT CTTTTCCTCG GGAGGTTACC
0001 ttgtttctcg cacaagaaat tattcgtaaa aaacgtgatg gtcatgcgct gagcgatgaa gaaattcgtt
0071 tctttatcaa cggtattcgc gacaacacta tctccgaagg gcagattgcc gccctcgcga tgaccatttt
0141 cttccacgat atgacaatgc ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc
0211 gactggaaaa gcctgcatct gaatggcccg attgttgata aacactccac cggtggcgtc ggcgatgtga
0281 cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg atgatctctg gtcgcggcct
0351 cggtcatact ggcggtacgc tcgacaaact ggaatccatc cctggcttcg acattttccc ggatgacaac
0421 cgtttccgcg aaattattaa agacgtcggc gtggcgatta tcggtcagac cagttcactg gctccggctg
0491 ataaacgttt ctacgcgacc cgtgatatta ccgcaaccgt ggactccatc ccgctgatca ccgcctctat
0561 tctggcgaag aaacttgcgg aaggtctgga cgcgctggtg atggacgtga aagtgggtag cggcgcgttt
0631 atgccgacct acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc gctggcgtgc
0701 gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca ggtaacgcgg ttgaagttcg
0771 tgaagcggtg cagttcctga cgggtgaata tcgtaacccg cgtctgtttg atgtcacgat ggcgctgtgc
0841 gtggagatgc tgatctccgg caaactggcg aaagatgacg ccgaagcgcg cgcgaaattg caggcggtgc
0911 tggacaacgg taaagcggca gaagtctttg gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt
0981 tgagaactac gcgaagtatc tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt
1051 gtcagtgaaa tggatacccg cgcgctgggg atggcagtgg ttgcaatggg cggcggacgc cgtcaggcat
1121 ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc gaccaggtag acggtcagcg
1191 tccgctggcg gttatccacg cgaaagacga aaacaactgg caggaagcgg cgaaagcggt gaaagcggca
1261 attaaacttg ccgataaagc accggaaagc acaccaactg tctatcgccg tatcagcgaa taaCGGTATA
1331 CTGATCTGAT CATTTAAATT TGAAGCACTG AGTACGGAGA ACATATGAAA CGTGCATTTA TTATGGTGCT
1401 GGACTCATTC GGCATCGGCG CTA
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