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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
Mol Gen Genet. 1996;250(4):455-65.
Surface signaling in transcriptional regulation of the ferric citrate transport system of Escherichia coli: mutational analysis of the alternative sigma factor FecI supports its essential role in fec transport gene transcription.
Ochs M, Angerer A, Enz S, Braun V.
(
8602163
)
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Deletion
|
1
(LD)
,
OCR20,24-1 (LD 1-1_CRS)
(D)
,
OCR20,24-2 (LD 1-1_P/O)
(D)
,
OCR21
(D)
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|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
fecA
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Related strains
|
|
Strains Search
|
Search strains by gene name:
fecA
Search strains by all related name:
fecA b4291 ECK4281 f774 JW4251
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General information (Go to Linear View: )
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Gene Name
|
fecA
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|
Alternative name
|
b4291,ECK4281,f774,JW4251
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Location, Length
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4,512,376 - 4,514,700
(
-
)
;
97.26
min
;
2325
(bp) ,
774
(aa)
|
|
Product
|
KpLE2 phage-like element; ferric citrate outer membrane transporter
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Operon Name
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fecABCDE
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Note
|
outer membrane receptor; citrate-dependent iron transport, outer membrane receptor; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0009279 - cell outer membrane; GO_process: GO:0006826 - iron ion transport
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Function
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membrane; Transport of small molecules: Cations
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Gene Ontology
|
GO:0004872
;
receptor activity ( fecA )
GO:0005215
;
transporter activity ( fecA )
GO:0005506
;
iron ion binding ( fecA )
GO:0006810
;
transport ( fecA )
GO:0006811
;
ion transport ( fecA )
GO:0006826
;
iron ion transport ( fecA )
GO:0009279
;
cell outer membrane ( fecA )
GO:0009935
;
nutrient import ( fecA )
GO:0015343
;
siderophore-iron transmembrane transporter activity ( fecA )
GO:0015891
;
siderophore transport ( fecA )
GO:0016020
;
membrane ( fecA )
GO:0019867
;
outer membrane ( fecA )
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PID
|
1790743
|
EC number
(KEGG Pathway)
|
|
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
fecA
(
EG10286
)
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Number of removed
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33 aa cleaved
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SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
FECA_ECOLI
(
P13036
)
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- Protein name
|
Iron(III) dicitrate transport protein fecA precursor.
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- Synonyms
|
|
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- Gene name
|
Name=fecA; OrderedLocusNames=b4291;
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- Comments
|
FUNCTION:FecA is the outer membrane receptor protein in the iron(III) dicitrate transport system.
SUBCELLULAR LOCATION:Outer membrane.
INDUCTION:For induction, the tonB and the exbB proteins have to be active. Regulation by the iron level mediated by the fur protein and induction by citrate plus iron suggest that the iron(III) dicitrate complex must enter the periplasm where it binds to a transmembrane protein, which regulates transcription of the fec genes directly or via a further inductor.
SIMILARITY:Belongs to the tonB-dependent receptor family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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fecA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain A, Crystal Structure Of The Outer Membrane Transporter Feca
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Iron
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PSI-BLAST
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Iron
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nr
(database updated :
2007.02.20
)
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BLAST
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KpLE2 phage-like element; ferric citrate outer membrane transporter [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
TONB_DEPENDENT_REC_1
TONB_DEPENDENT_REC_2
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MMBR References
|
Mol Microbiol. 1995;18(1):163-74.
Transcriptional regulation of ferric citrate transport in Escherichia coli K-12. Fecl belongs to a new subfamily of sigma 70-type factors that respond to extracytoplasmic stimuli.
Angerer A, Enz S, Ochs M, Braun V.
(
8596456
)
|
Eur J Biochem. 1981;117:431-437
Citrate-dependent iron transport system in Escherichia coli.
Hussein, S., K. Hantke, and V. Braun.
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J Bacteriol. 1988;170(6):2716-24.
Genetics of the iron dicitrate transport system of Escherichia coli.
Pressler U, Staudenmaier H, Zimmermann L, Braun V.
(
2836368
)
|
J Bacteriol. 1990;172(12):6749-58.
Novel two-component transmembrane transcription control: regulation of iron dicitrate transport in Escherichia coli K-12.
Van Hove B, Staudenmaier H, Braun V.
(
2254251
)
|
J Bacteriol. 1992;174(11):3838-9.
Localization of the entire fec region at 97.3 minutes on the Escherichia coli K-12 chromosome.
Veitinger S, Braun V.
(
1592838
)
|
J Bacteriol. 1981;145(1):156-63.
Ferric citrate transport in Escherichia coli requires outer membrane receptor protein fecA.
Wagegg W, Braun V.
(
7007312
)
|
J Bacteriol. 1984;159(1):271-7.
Exogenous induction of the iron dicitrate transport system of Escherichia coli K-12.
Zimmermann L, Hantke K, Braun V.
(
6376472
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MTPLRVFRKT TPLVNTIRLS LLPLAGLSFS AFAAQVNIAP GSLDKALNQY AAHSGFTLSV DASLTRGKQS
0071 NGLHGDYDVE SGLQQLLDGS GLQVKPLGNN SWTLEPAPAP KEDALTVVGD WLGDARENDV FEHAGARDVI
0141 RREDFAKTGA TTMREVLNRI PGVSAPENNG TGSHDLAMNF GIRGLNPRLA SRSTVLMDGI PVPFAPYGQP
0211 QLSLAPVSLG NMDAIDVVRG GGAVRYGPQS VGGVVNFVTR AIPQDFGIEA GVEGQLSPTS SQNNPKETHN
0281 LMVGGTADNG FGTALLYSGT RGSDWREHSA TRIDDLMLKS KYAPDEVHTF NSLLQYYDGE ADMPGGLSRA
0351 DYDADRWQST RPYDRFWGRR KLASLGYQFQ PDSQHKFNIQ GFYTQTLRSG YLEQGKRITL SPRNYWVRGI
0421 EPRYSQIFMI GPSAHEVGVG YRYLNESTHE MRYYTATSSG QLPSGSSPYD RDTRSGTEAH AWYLDDKIDI
0491 GNWTITPGMR FEHIESYQNN AITGTHEEVS YNAPLPALNV LYHLTDSWNL YANTEGSFGT VQYSQIGKAV
0561 QSGNVEPEKA RTWELGTRYD DGALTAEMGL FLINFNNQYD SNQTNDTVTA RGKTRHTGLE TQARYDLGTL
0631 TPTLDNVSIY ASYAYVNAEI REKGDTYGNL VPFSPKHKGT LGVDYKPGNW TFNLNSDFQS SQFADNANTV
0701 KESADGSTGR IPGFMLWGAR VAYDFGPQMA DLNLAFGVKN IFDQDYFIRS YDDNNKGIYA GQPRTLYMQG
0771 SLKF
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tttcaccact gtaaggaaaa taattcttat
-070 ttcgattgtc ctttttaccc ttctcgttcg actcatagct gaacacaaca aaaatgatga tggggaaggt
0001 atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg ttaacaccat tcgcctgagc ctgctgccgc
0071 tggccggtct ctcgttttcc gcttttgctg cacaggttaa tatcgcaccg ggatcgctcg ataaagcgct
0141 caatcagtat gccgcacaca gcggatttac cctctcggtt gacgccagcc tgacgcgcgg caagcagagc
0211 aacggcctgc acggcgatta cgacgtcgag agcggcctgc aacaactgct ggacggcagc ggactgcagg
0281 taaaaccgct gggaaataac agctggacgc tggagcccgc gcccgcacca aaagaagatg ccctgaccgt
0351 ggtcggcgac tggctgggtg atgcgcgtga aaacgacgta tttgaacatg ctggcgcgcg tgacgtgatc
0421 cgccgtgagg atttcgccaa aaccggcgca accaccatgc gtgaggtact taaccgcatc cctggcgtca
0491 gcgcgccgga aaacaacggc accggcagcc acgacctggc gatgaacttt ggcatccggg gcctgaaccc
0561 gcgcctcgcc agccgctcga ccgtcctgat ggacggcatc cccgtcccct tcgcccctta cggtcagccg
0631 cagctttcac tggctcccgt ttcgctcggc aacatggatg ccattgacgt ggtacgcggt ggtggtgcgg
0701 tgcgttacgg accgcagagc gtgggcggcg tggtgaactt tgttacccgt gccattccgc aggactttgg
0771 tatcgaggcg ggcgtggaag gtcagctcag cccaacctct tcacaaaaca acccgaaaga gacgcacaac
0841 ctgatggtgg gcggcacagc ggacaacggt tttggcaccg cgctgctcta ctccggcacg cgcggcagtg
0911 actggcgcga gcacagcgcc acccgcatcg acgacctgat gctgaaaagc aaatatgcgc cggatgaggt
0981 gcacaccttc aacagcctgc tgcaatatta cgacggtgaa gccgacatgc ccggtggcct gtctcgcgcg
1051 gattacgacg ccgatcgctg gcaatccacc cgcccgtatg accgcttctg gggtcgtcgc aagctggcga
1121 gcctgggcta ccagttccag ccagacagcc agcataaatt caacattcag gggttctaca cccaaaccct
1191 gcgcagcggc tacctggagc aaggcaaacg catcaccctc tcgccgcgta actactgggt gcgcggtatt
1261 gagccacgct acagccagat ctttatgatc ggcccttccg cgcacgaagt gggcgtgggc tatcgctatt
1331 tgaatgaatc aacgcatgaa atgcgttact acaccgccac cagcagcggg cagttgccgt ccggctcaag
1401 cccttacgac cgcgatacgc gttccggcac cgaggcgcac gcctggtatc tggatgacaa aatcgacatc
1471 ggcaactgga ccatcacgcc gggtatgcgt ttcgaacata tcgagtcata ccagaacaac gccatcacag
1541 gcacgcacga agaagtgagc tataacgcac cgcttccggc gttgaacgtg ctctatcacc tgactgacag
1611 ctggaatctt tatgcaaaca ctgaaggctc gttcggcacc gtacagtaca gccagattgg caaggctgtg
1681 caaagcggca atgttgaacc ggaaaaagcg cgaacctggg aactcggtac ccgctacgac gacggcgcgc
1751 tgacggcgga aatggggctg ttcctgatta actttaacaa tcagtacgac tccaaccaga ccaacgacac
1821 cgtcactgca cgtggcaaaa cgcgccatac cgggctggaa acgcaggcac gttacgatct gggtacgcta
1891 acgccaacgc ttgataacgt ttccatctac gccagctatg cgtatgtgaa cgcggaaatc cgcgagaaag
1961 gcgacaccta cggcaatctg gtaccattct ccccgaaaca taaaggcacg ctgggcgtgg actacaagcc
2031 aggaaactgg acgttcaatc tgaacagcga tttccagtcc agccagtttg cggataacgc caatacggtg
2101 aaagagagcg ccgacggcag taccggccgc attcccggct tcatgctctg gggcgcacgc gtggcgtatg
2171 actttggccc gcagatggca gatctgaacc tggcattcgg tgtgaaaaac atcttcgacc aggactactt
2241 catccgctct tatgacgaca acaacaaagg catctatgca ggccagccgc gcacgctgta tatgcagggg
2311 tcgttgaagt tctgatgtgt taacgcccgg cttgccgggc ttttagctgg aatgtgatta tgttggcatt
2381 tatccgtttt ctttttgcag gcctgctgct ggtgatcagc cacgc
|
