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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCR16,17-3 (BK2-17 / AK1-17)
(D)
,
OCR17
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
miaA
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Related strains
|
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Strains Search
|
Search strains by gene name:
miaA
Search strains by all related name:
miaA b4171 ECK4167 JW4129 trpX
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General information (Go to Linear View: )
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Gene Name
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miaA
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Alternative name
|
b4171,ECK4167,JW4129,trpX
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Location, Length
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4,397,275 - 4,398,225
(
+
)
;
94.78
min
;
951
(bp) ,
316
(aa)
|
|
Product
|
delta(2)-isopentenylpyrophosphate tRNA-adenosine transferase
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Operon Name
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yjeFE-amiB-mutL-miaA-hfq-hflXKC
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009451 - RNA modification
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( miaA )
GO:0004811
;
tRNA isopentenyltransferase activity ( miaA )
GO:0005524
;
ATP binding ( miaA )
GO:0008033
;
tRNA processing ( miaA )
GO:0016740
;
transferase activity ( miaA )
GO:0016779
;
nucleotidyltransferase activity ( miaA )
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PID
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1790613
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EC number
(KEGG Pathway)
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2.5.1.75
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
|
MIAA_ECOLI
(
P16384
)
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- Protein name
|
tRNA delta(2)-isopentenylpyrophosphate transferase
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- Synonyms
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EC 2.5.1.8, IPP transferase, Isopentenyl-diphosphate:tRNA isopentenyltransferase, IPTase, IPPT
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- Gene name
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Name=miaA; Synonyms=trpX; OrderedLocusNames=b4171;
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- Comments
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FUNCTION:Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A) adjacent to the anticodon of several tRNA species.
CATALYTIC ACTIVITY:Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine.
SUBUNIT:Homodimer (Potential).
INDUCTION:By 2-aminopurine.
MISCELLANEOUS:Specific tRNA undermodification may be a switch to higher mutation frequency when cells are subject to environmental stresses other than treatment with mutagens.
SIMILARITY:Belongs to the IPP transferase family.
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Entry name(Acc.no)
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MIAA_ECOL6
(
Q8CXV3
)
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- Protein name
|
tRNA delta(2)-isopentenylpyrophosphate transferase
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- Synonyms
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EC 2.5.1.8, IPP transferase, Isopentenyl-diphosphate:tRNA isopentenyltransferase, IPTase, IPPT
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- Gene name
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Name=miaA; OrderedLocusNames=c5255;
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- Comments
|
FUNCTION:Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A) adjacent to the anticodon of several tRNA species (By similarity).
CATALYTIC ACTIVITY:Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine.
SIMILARITY:Belongs to the IPP transferase family.
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Entry name(Acc.no)
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MIAA_ECO57
(
Q8XDN3
)
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- Protein name
|
tRNA delta(2)-isopentenylpyrophosphate transferase
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- Synonyms
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EC 2.5.1.8, IPP transferase, Isopentenyl-diphosphate:tRNA isopentenyltransferase, IPTase, IPPT
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- Gene name
|
Name=miaA; OrderedLocusNames=z5778, ECs5147;
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- Comments
|
FUNCTION:Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A) adjacent to the anticodon of several tRNA species (By similarity).
CATALYTIC ACTIVITY:Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine.
SIMILARITY:Belongs to the IPP transferase family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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miaA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain E, Crystal Structures Of The Hslvu Peptidase-Atpase Complex Reveal An Atp-Dependent Proteolysis Mechanism
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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tRNA delta
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PSI-BLAST
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tRNA delta
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|
nr
(database updated :
2007.02.20
)
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BLAST
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tRNA delta(2)-isopentenylpyrophosphate transferase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
RGD
ATP_GTP_A
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MMBR References
|
J Bacteriol. 1984;158(2):762-3.
Genetic mapping of pheU, an Escherichia coli gene for phenylalanine tRNA.
Gallagher PJ, Schwartz I, Elseviers D.
(
6373735
)
|
J Bacteriol. 1996;178:5719-5731
Transcription of the mutL repair, miaA tRNA modification, hfq pleiotropic regulator, and hflA region protease genes of Escherichia coli K-12 from clustered E sigma32-specific promoters during heat shock.
Tsui, H.-C. T., G. Feng, and M. E. Winkler.
|
Mol Microbiol. 1994;11(1):189-202.
The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase.
Tsui HC, Zhao G, Feng G, Leung HC, Winkler ME.
(
7511774
)
|
Mol Microbiol. 1994;13(1):35-49.
Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12.
Tsui HC, Leung HC, Winkler ME.
(
7984093
)
|
J Bacteriol. 1993;175:7209-7215
tRNA modification activity is necessary for Tet(M)-mediated tetracycline resistance.
Burdett, V.
|
J Bacteriol. 1988;170(9):4147-52.
Molecular cloning of the Escherichia coli miaA gene involved in the formation of delta 2-isopentenyl adenosine in tRNA.
Caillet J, Droogmans L.
(
3045085
)
|
J Bacteriol. 1989;171(6):3233-46.
Genetic and physiological relationships among the miaA gene, 2-methylthio-N6-(delta 2-isopentenyl)-adenosine tRNA modification, and spontaneous mutagenesis in Escherichia coli K-12.
Connolly DM, Winkler ME.
(
2656644
)
|
J Bacteriol. 1991;173(5):1711-21.
Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations.
Connolly DM, Winkler ME.
(
1999389
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSDISKASLP KAIFLMGPTA SGKTALAIEL RKILPVELIS VDSALIYKGM DIGTAKPNAE ELLAAPHRLL
0071 DIRDPSQAYS AADFRRDALA EMADITAAGR IPLLVGGTML YFKALLEGLS PLPSADPEVR ARIEQQAAEQ
0141 GWESLHRQLQ EVDPVAAARI HPNDPQRLSR ALEVFFISGK TLTELTQTSG DALPYQVHQF AIAPASRELL
0211 HQRIEQRFHQ MLASGFEAEV RALFARGDLH TDLPSIRCVG YRQMWSYLEG EISYDEMVYR GVCATRQLAK
0281 RQITWLRGWE GVHWLDSEKP EQARDEVLQV VGAIAG
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CTGGCGGACG TGGAACGGTT ATGTCCGCAA
-070 CTTGTGAAAA CGCCGCCGGG TGGTCTGTTA CAATCTGTTG ATTTACATCC GGCGATAAAA GCCCTGAAAG
0001 atgagtgata tcagtaaggc gagcctgcct aaggcgattt ttttgatggg gccgacggcc tccggtaaaa
0071 cggcgttagc cattgagctg cgtaaaattt taccagtaga gttgataagc gttgattctg cccttattta
0141 caaagggatg gatatcggga cggcgaagcc gaacgctgaa gagttactcg ccgcgccgca ccgattgctg
0211 gatattcgcg atccgtcgca ggcttactcg gctgctgatt ttcgccgcga tgcgctggcg gaaatggccg
0281 atatcaccgc ggcggggcgg atcccactgt tagtgggcgg tacgatgttg tatttcaagg cattgctgga
0351 agggttgtcg ccgctaccgt cggcagaccc ggaagtacgg gccagaattg agcaacaggc ggcagagcaa
0421 ggttgggagt cattgcatcg tcaacttcag gaggtagatc cggttgcggc agcaaggatt catccaaatg
0491 atccacaaag gctttcccgg gcactggaag tttttttcat ttcgggtaaa actttaacgg aactgacgca
0561 aacgtcagga gacgctctac cgtatcaggt gcatcagttc gccatcgccc cggcgagccg tgaactgctc
0631 catcaacgca ttgagcagcg ttttcatcag atgttggctt caggttttga agcagaagtc cgggcgcttt
0701 ttgcccgagg agatttgcat acggacttgc cttccattcg ttgcgtgggt tatcgccaga tgtggtctta
0771 ccttgaaggc gaaatctcat acgatgaaat ggtttatcga ggtgtttgcg ccacgagaca gttggcgaag
0841 cggcagataa cctggctgcg tggttgggaa ggggttcact ggcttgacag tgaaaaacca gaacaggcgc
0911 gtgacgaagt attacaggtt gttggtgcta tcgcaggctg aATGTGTACA ATTGAGACGT ATCGTGCGCA
0981 ATTTTTTCAG AATCGAAAGG TTCAAAGTAC AAATAAGCAT ATAAGGAAAA GAGAGAATGG CTAAGGGGCA
1051 A
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