MG1655
W3110
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Gene Report : malZ
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR39 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  malZ
Related strains
     Strains Search Search strains by gene name:  malZ   Search strains by all related name:  malZ b0403 ECK0397 JW0393

General information  (Go to Linear View:)
 Gene Name malZ  
 Alternative name b0403,ECK0397,JW0393  
 Location, Length 421,742 - 423,556 (  +  ) ;   9.09 min ; 1815 (bp) ,   604 (aa) Go to Linear View
 Product maltodextrin glucosidase  
 Operon Name malZ  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0000272 - polysaccharide catabolic process  
 Function enzyme; Degradation of small molecules: Carbon compounds  
 Gene Ontology GO:0000023 ; maltose metabolic process ( malZ )
GO:0003824 ; catalytic activity ( malZ )
GO:0004558 ; alpha-glucosidase activity ( malZ )
GO:0005737 ; cytoplasm ( malZ )
GO:0005975 ; carbohydrate metabolic process ( malZ )
GO:0008152 ; metabolic process ( malZ )
GO:0016787 ; hydrolase activity ( malZ )
GO:0016798 ; hydrolase activity, acting on glycosyl bonds ( malZ )
GO:0043169 ; cation binding ( malZ )
 PID 345297171  
 EC number
  (KEGG Pathway)   		3.2.1.20  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) MALZ_ECOLI ( P21517 )
    -  Protein name Maltodextrin glucosidase  
    -  Synonyms EC 3.2.1.20, Alpha-glucosidase  
    -  Gene name Name=malZ; OrderedLocusNames=b0403;  
    -  Comments
  • FUNCTION:May play a role in regulating the intracellular level of maltotriose. Cleaves glucose from the reducing end of maltotriose and longer maltodextrins with a chain length of up to 7 glucose units.
  • CATALYTIC ACTIVITY:Hydrolysis of terminal, non-reducing 1,4-linked D-glucose residues with release of D-glucose.
  • SUBUNIT:Monomer.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • INDUCTION:Under positive control of malT.
  • SIMILARITY:Belongs to family 13 of glycosyl hydrolases.  
    •   botton Entry name(Acc.no) Q8FKC7 ( Q8FKC7 )
        -  Protein name Maltodextrin glucosidase  
        -  Synonyms EC 3.2.1.20  
        -  Gene name Name=malZ; OrderedLocusNames=c0513;  
        -  Comments
  • FUNCTION:Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid (By similarity).
  • CATALYTIC ACTIVITY:(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH.
  • SIMILARITY:Belongs to the ketopantoate reductase family.  
    •   botton Entry name(Acc.no) Q8XE98 ( Q8XE98 )
        -  Protein name Maltodextrin glucosidase.  
        -  Synonyms  
        -  Gene name Name=malZ; OrderedLocusNames=z0501;  
        -  Comments
  • SUBCELLULAR LOCATION:Integral membrane protein (By similarity).  

    •  Linear View (Whole Mode)
    View Location
       410.0  –  435.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP malZ (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Crystal Structure Of A Maltogenic Amylase  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Maltodextrin glucosidase  
             PSI-BLAST Maltodextrin glucosidase
        nr     (database updated : 2007.02.20 )
             BLAST maltodextrin glucosidase [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

    Other Cross-Reference
        EcoCyc malZ 

    MMBR References
    J Bacteriol. 1992;174(5):1709-10.
    Alignment of genes from the 9-minute region (araJ to tsx) of the Escherichia coli K-12 linkage map to the physical map.
    Lucht JM, Boos W, Bremer E. ( 1537816 )
    J Biol Chem. 1996;271:10681-10689
    The MalT-dependent and malZ-encoded maltodextrin glucosidase of Escherichia coli can be converted into a dextrinyltransferase by a single mutation.
    Peist, R., C. Schneider-Fresenius, and W. Boos.
    J Biol Chem. 1991;266:19450-19458
    The malZ gene of Escherichia coli, a member of the maltose regulon, encodes a maltodextrin glucosidase.
    Tapio, S., F. Yeh, H. Shuman, and W. Boos.

    Sequences
    Amino acid
    FASTA format 
    0001 MLNAWHLPVP PFVKQSKDQL LITLWLTGED PPQRIMLRTE HDNEEMSVPM HKQRSQPQPG VTAWRAAIDL 
0071 SSGQPRRRYS FKLLWHDRQR WFTPQGFSRM PPARLEQFAV DVPDIGPQWA ADQIFYQIFP DRFARSLPRE 
0141 AEQDHVYYHH AAGQEIILRD WDEPVTAQAG GSTFYGGDLD GISEKLPYLK KLGVTALYLN PVFKAPSVHK 
0211 YDTEDYRHVD PQFGGDGALL RLRHNTQQLG MRLVLDGVFN HSGDSHAWFD RHNRGTGGAC HNPESPWRDW 
0281 YSFSDDGTAL DWLGYASLPK LDYQSESLVN EIYRGEDSIV RHWLKAPWNM DGWRLDVVHM LGEAGGARNN 
0351 MQHVAGITEA AKETQPEAYI VGEHFGDARQ WLQADVEDAA MNYRGFTFPL WGFLANTDIS YDPQQIDAQT 
0421 CMAWMDNYRA GLSHQQQLRM FNQLDSHDTA RFKTLLGRDI ARLPLAVVWL FTWPGVPCIY YGDEVGLDGK 
0491 NDPFCRKPFP WQVEKQDTAL FALYQRMIAL RKKSQALRHG GCQVLYAEDN VVVFVRVLNQ QRVLVAINRG 
0561 EACEVVLPAS PFLNAVQWQC KEGHGQLTDG ILALPAISAT VWMN
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             ATCCGCCCCC GTCCTCCTCC CCCAAATATC 
-070 CTTCAGATGA TGAGTGATCC TGCATTAGGC TATGGCAAGG TGATCAGATT TTCATCACAG GGGAATTATG 
0001 atgttaaatg catggcacct gccggtgccc ccatttgtta aacaaagcaa agatcaactg ctcattacac 
0071 tgtggctgac gggcgaagac ccaccgcagc gcattatgct gcgtacagaa cacgataacg aagaaatgtc 
0141 agtaccgatg cataagcagc gcagtcagcc gcagcctggc gtcaccgcat ggcgtgcggc gattgatctc 
0211 tccagcggac aaccccggcg gcgttacagt ttcaaactgc tgtggcacga tcgccagcgt tggtttacac 
0281 cgcagggctt cagccgaatg ccgccggcac gactggagca gtttgccgtc gatgtaccgg atatcggccc 
0351 acaatgggct gcggatcaga ttttttatca gatcttccct gatcgttttg cgcgtagtct tcctcgtgaa 
0421 gctgaacagg atcatgtcta ttaccatcat gcagccggac aagagatcat cttgcgtgac tgggatgaac 
0491 cggtcacggc gcaggcgggc ggatcaacgt tctatggcgg cgatctggac gggataagcg aaaaactgcc 
0561 gtatctgaaa aagcttggcg tgacagcgct gtatctcaat ccggtgttta aagctcccag cgtacataaa 
0631 tacgataccg aggattatcg ccatgtcgat ccgcagtttg gcggtgatgg ggcgttgctg cgtttgcgac 
0701 acaatacgca gcagctggga atgcggctgg tgctggacgg cgtgtttaac cacagtggcg attcccatgc 
0771 ctggtttgac aggcataatc gtggcacggg tggtgcttgt cacaaccccg aatcgccctg gcgcgactgg 
0841 tactcgttta gtgatgatgg cacggcgctc gactggcttg gctatgccag cttgccgaag ctggattatc 
0911 agtcggaaag tctggtgaat gaaatttatc gcggggaaga cagtattgtc cgccactggc tgaaagcgcc 
0981 gtggaatatg gacggctggc ggctggatgt ggtgcatatg ctgggggagg cgggtggggc gcgcaataat 
1051 atgcagcacg ttgccgggat caccgaagcg gcgaaagaaa cccagccgga agcgtatatt gtcggcgaac 
1121 attttggcga tgcacggcaa tggttacagg ccgatgtgga agatgccgcc atgaactatc gtggcttcac 
1191 attcccgttg tggggatttc ttgccaatac cgatatctct tacgatccgc agcaaattga tgcccaaacc 
1261 tgtatggcct ggatggataa ttaccgcgca gggctttctc atcaacaaca attacgtatg tttaatcagc 
1331 tcgacagcca cgatactgcg cgatttaaaa cgctgctcgg tcgggatatt gcgcgcctgc cgctggcggt 
1401 ggtctggctg ttcacctggc ctggtgtacc gtgcatttat tacggtgatg aagtaggact ggatggcaaa 
1471 aacgatccgt tttgccgtaa accgttcccc tggcaggtgg aaaagcagga tacggcgtta ttcgcgctgt 
1541 accagcgaat gattgcgctg cgtaagaaaa gtcaggcgct acgtcatggc ggctgtcagg tgctgtatgc 
1611 ggaagataac gtggtggtat ttgtccgcgt gctgaatcag caacgtgtac tggtggcaat caaccgtggc 
1681 gaggcctgtg aagtggtgct acccgcgtca ccgtttctca atgccgtgca atggcaatgc aaagaagggc 
1751 atgggcaact gactgacggg attctggctt tgcctgccat ttcggctacg gtatggatga actaaCGTTT 
1821 TATAACGCCT TGCGTGACGC CTGCGCCATC ATCCGCGGAT AAAACTGCCA GAAGCGGGTT TCGAGGGCGT 
1891 CATAATGAGC GTCTAAATCG TACCA