MG1655
W3110
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Gene Report : argH
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR10 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  argH
Related strains
     Strains Search Search strains by gene name:  argH   Search strains by all related name:  argH b3960 ECK3951 JW3932

General information  (Go to Linear View:)
 Gene Name argH  
 Alternative name b3960,ECK3951,JW3932  
 Location, Length 4,154,873 - 4,156,246 (  +  ) ;   89.55 min ; 1374 (bp) ,   457 (aa) Go to Linear View
 Product argininosuccinate lyase  
 Operon Name argCBH  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006526 - arginine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Arginine  
 Gene Ontology GO:0003824 ; catalytic activity ( argH )
GO:0004056 ; argininosuccinate lyase activity ( argH )
GO:0005737 ; cytoplasm ( argH )
GO:0006526 ; arginine biosynthetic process ( argH )
GO:0008652 ; amino acid biosynthetic process ( argH )
GO:0016829 ; lyase activity ( argH )
GO:0042450 ; arginine biosynthetic process via ornithine ( argH )
 PID 1790398  
 EC number
  (KEGG Pathway)   		4.3.2.1  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) ARLY_ECOLI ( P11447 ; Q47060 )
    -  Protein name Argininosuccinate lyase  
    -  Synonyms EC 4.3.2.1, Arginosuccinase, ASAL  
    -  Gene name Name=argH; OrderedLocusNames=b3960;  
    -  Comments
  • CATALYTIC ACTIVITY:N-(L-arginino)succinate = fumarate + L-arginine.
  • PATHWAY:Arginine biosynthesis; eighth (last) step.
  • SUBCELLULAR LOCATION:Cytoplasmic (Probable).
  • SIMILARITY:Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.  
    •   botton Entry name(Acc.no) ARLY_ECOL6 ( Q8FB96 )
        -  Protein name Argininosuccinate lyase  
        -  Synonyms EC 4.3.2.1, Arginosuccinase, ASAL  
        -  Gene name Name=argH; OrderedLocusNames=c4919;  
        -  Comments
  • CATALYTIC ACTIVITY:N-(L-arginino)succinate = fumarate + L-arginine.
  • PATHWAY:Arginine biosynthesis; eighth (last) step.
  • SUBCELLULAR LOCATION:Cytoplasmic (Probable).
  • SIMILARITY:Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.  
    •   botton Entry name(Acc.no) ARLY_ECO57 ( Q8X730 )
        -  Protein name Argininosuccinate lyase  
        -  Synonyms EC 4.3.2.1, Arginosuccinase, ASAL  
        -  Gene name Name=argH; OrderedLocusNames=z5518, ECs4889;  
        -  Comments
  • CATALYTIC ACTIVITY:N-(L-arginino)succinate = fumarate + L-arginine.
  • PATHWAY:Arginine biosynthesis; eighth (last) step.
  • SUBCELLULAR LOCATION:Cytoplasmic (Probable).
  • SIMILARITY:Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.  

    •  Linear View (Whole Mode)
    View Location
       4145.0  –  4170.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP argH (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Crystal Structure Of S283a Duck Delta 2 Crystallin Mutant  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Argininosuccinate lyase  
             PSI-BLAST Argininosuccinate lyase
        nr     (database updated : 2007.02.20 )
             BLAST argininosuccinate lyase [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    ATP_GTP_A    FUMARATE_LYASES     

    Other Cross-Reference
        COG COG0165E 
        EcoCyc argH 

    MMBR References
    J Bacteriol. 1992;174(11):3836-7.
    Physical map location of the argFGH operon of Escherichia coli.
    Hendrickson W, Rudd KE. ( 1592837 )
    In K. D. MacDonald (ed.), Proceedings of the Second International Symposium on Genetics and Industrial Microorganisms. Academic Press, London, United Kingdom. 1976:369-389
    Genetic control of arginine metabolism in prokaryotes.
    Baumberg, S.
    Gene. 1979;8(1):25-34.
    Clustered arg genes on a BamHI segment of the Escherichia coli chromosome.
    Moran MC, Mazaitis AJ, Vogel RH, Vogel HJ. ( 231541 )
    Gene. 1979;5(3):207-31.
    Cloning and endonuclease restriction analysis of argF and of the control region of the argECBH bipolar operon in Escherichia coli.
    Crabeel M, Charlier D, Cunin R, Glansdorff N. ( 381103 )

    Sequences
    Amino acid
    FASTA format 
    0001 MALWGGRFTQ AADQRFKQFN DSLRFDYRLA EQDIVGSVAW SKALVTVGVL TAEEQAQLEE ALNVLLEDVR 
0071 ARPQQILESD AEDIHSWVEG KLIDKVGQLG KKLHTGRSRN DQVATDLKLW CKDTVSELLT ANRQLQSALV 
0141 ETAQNNQDAV MPGYTHLQRA QPVTFAHWCL AYVEMLARDE SRLQDALKRL DVSPLGCGAL AGTAYEIDRE 
0211 QLAGWLGFAS ATRNSLDSVS DRDHVLELLS AAAIGMVHLS RFAEDLIFFN TGEAGFVELS DRVTSGSSLM 
0281 PQKKNPDALE LIRGKCGRVQ GALTGMMMTL KGLPLAYNKD MQEDKEGLFD ALDTWLDCLH MAALVLDGIQ 
0351 VKRPRCQEAA QQGYANATEL ADYLVAKGVP FREAHHIVGE AVVEAIRQGK PLEDLPLSEL QKFSQVIDED 
0421 VYPILSLQSC LDKRAAKGGV SPQQVAQAIA FAQARLG
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             GTTTAACGGT ATGCCGATGG GTACGCGGAT 
-070 TTTAGCTTAA GTTTTGTTGG CCGGAGGCGC AGCTTTCCGG CATTGAATTT CAAAATAAGG AAACAGAGTT 
0001 atggcacttt ggggcgggcg ttttacccag gcagcagatc aacggttcaa acaattcaac gactcactgc 
0071 gctttgatta ccgtctggcg gagcaggata ttgttggctc tgtggcctgg tccaaagccc tggtcacggt 
0141 aggcgtgtta accgcagaag agcaggcgca actggaagag gcgctgaacg tgttgctgga agatgttcgc 
0211 gccaggccac aacaaatcct tgaaagcgac gccgaagata tccatagctg ggtggaaggc aaactgatcg 
0281 acaaagtggg ccagttaggc aaaaagctgc ataccgggcg tagccgtaat gatcaggtag cgactgacct 
0351 gaaactgtgg tgcaaagata ccgttagcga gttactgacg gctaaccggc agctgcaatc ggcgctggtg 
0421 gaaaccgcac aaaacaatca ggacgcggta atgccaggtt acactcacct gcaacgcgcc cagccggtga 
0491 cgttcgcgca ctggtgcctg gcctatgttg agatgctggc gcgtgatgaa agccgtttgc aggatgcgct 
0561 taagcgtctg gatgtcagcc cgctaggctg tggcgcgctg gcgggaacgg cctatgaaat cgaccgtgaa 
0631 cagttagcag gctggctggg ctttgcttcg gcgacccgta acagtctcga cagcgtttct gaccgtgacc 
0701 atgtgttgga actgctttct gctgccgcta tcggcatggt gcatctgtcg cgttttgctg aagatctgat 
0771 tttctttaac accggcgaag cggggtttgt ggagctttct gaccgcgtga cttccggttc atcattaatg 
0841 ccgcagaaga aaaacccgga tgcgctggag ctgattcgcg gtaaatgcgg ccgggtgcag ggggcgttaa 
0911 ccggcatgat gatgacgctg aaaggtttgc cgctggctta caacaaagat atgcaggaag acaaagaagg 
0981 tctgttcgac gcgctcgata cctggctgga ctgcctgcat atggcggcgc tggtgctgga cggcattcag 
1051 gtgaaacgtc cacgttgcca ggaagcggct cagcagggtt acgccaacgc caccgaactg gcggattatc 
1121 tggtggcgaa aggcgtaccg ttccgcgagg cgcaccatat tgttggtgaa gcggtggtgg aagccattcg 
1191 tcagggcaaa ccgctggaag atctgccgct cagtgagttg cagaaattca gtcaggtgat tgacgaagat 
1261 gtctatccga ttctgtcgct gcaatcgtgc ctcgacaagc gtgcggcaaa aggcggcgtc tcaccgcagc 
1331 aggtggcgca ggcgattgct tttgcgcagg ctcggttagg gtaaGAACAT TTATATGTAT AAATTTGAGC 
1401 CTGGCTTATC GCCGGGCTTT TTTATGGCAA AAAAAAGCGG ATCCTGGAGA TCCGCAAAAG TTCACGTTGG 
1471 CTTT