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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Mol Gen Genet. 1984;197(2):261-71.
Physical and genetic structure of the glpK-cpxA interval of the Escherichia coli K-12 chromosome.
Albin R, Silverman PM.
(
6097795
)
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Deletion
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OCR08-7
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
glpK
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Related strains
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Strains Search
|
Search strains by gene name:
glpK
Search strains by all related name:
glpK b3926 ECK3918 JW3897
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General information (Go to Linear View: )
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Gene Name
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glpK
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Alternative name
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b3926,ECK3918,JW3897
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Location, Length
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4,113,737 - 4,115,245
(
-
)
;
88.66
min
;
1509
(bp) ,
502
(aa)
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Product
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glycerol kinase
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Operon Name
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glpFKX
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Note
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GO_process: GO:0006071 - glycerol metabolic process
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Function
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enzyme; Central intermediary metabolism: Pool, multipurpose conversions
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Gene Ontology
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GO:0000166
;
nucleotide binding ( glpK )
GO:0004370
;
glycerol kinase activity ( glpK )
GO:0005524
;
ATP binding ( glpK )
GO:0005975
;
carbohydrate metabolic process ( glpK )
GO:0006071
;
glycerol metabolic process ( glpK )
GO:0006072
;
glycerol-3-phosphate metabolic process ( glpK )
GO:0008270
;
zinc ion binding ( glpK )
GO:0016301
;
kinase activity ( glpK )
GO:0016740
;
transferase activity ( glpK )
GO:0046872
;
metal ion binding ( glpK )
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PID
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1790361
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EC number
(KEGG Pathway)
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2.7.1.30
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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glpK
(
EG10398
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
|
GLPK_ECOLI
(
P08859
;
Q59381
)
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- Protein name
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Glycerol kinase
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- Synonyms
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EC 2.7.1.30, ATP:glycerol 3-phosphotransferase, Glycerokinase, GK
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- Gene name
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Name=glpK; OrderedLocusNames=b3926, z5471, ECs4851;
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- Comments
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FUNCTION:Key enzyme in the regulation of glycerol uptake and metabolism.
CATALYTIC ACTIVITY:ATP + glycerol = ADP + glycerol 3-phosphate.
COFACTOR:Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization.
ENZYME REGULATION:Activity of this regulatory enzyme is affected by several metabolites. The inhibition by fructose 1,6-biphosphate causes alterations in the quaternary structure of the enzyme. Phosphotransferase system phosphocarrier protein (IIIGlc or IIAGlc) inhibits activity of wild-type enzyme but activates the mutant enzyme.
PATHWAY:Glycerol utilization; rate-limiting step.
SUBUNIT:Homotetramer and homodimer (in equilibrium).
INDUCTION:By L-alpha-glycerol 3-phosphate.
MISCELLANEOUS:This enzyme is inactivated by modification with sulfhydryl reagents or the adenine nucleotide affinity label, 5'-P-(fluorosulfonyl)-benzoyl adenosine.
SIMILARITY:Belongs to the fucokinase / gluconokinase / glycerokinase / xylulokinase family.
DATABASE:NAME=Worthington enzyme manual; WWW="http://www.worthington-biochem.com/GKEC/".
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Entry name(Acc.no)
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GLPK_ECOL6
(
Q8FBC3
)
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- Protein name
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Glycerol kinase
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- Synonyms
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EC 2.7.1.30, ATP:glycerol 3-phosphotransferase, Glycerokinase, GK
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- Gene name
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Name=glpK; OrderedLocusNames=c4878;
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- Comments
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FUNCTION:Key enzyme in the regulation of glycerol uptake and metabolism.
CATALYTIC ACTIVITY:ATP + glycerol = ADP + glycerol 3-phosphate.
COFACTOR:Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization (By similarity).
PATHWAY:Glycerol utilization; first (rate-limiting) step.
SUBUNIT:Homotetramer and homodimer (in equilibrium) (By similarity).
SIMILARITY:Belongs to the fucokinase / gluconokinase / glycerokinase / xylulokinase family.
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MMBR References
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J Bacteriol. 1971;106(3):724-31.
Promoter-like mutant with increased expression of the glycerol kinase operon of Escherichia coli.
Berman-Kurtz M, Lin EC, Richey DP.
(
4934061
)
|
J Biol Chem. 1988;263:135-139
Escherichia coli glycerol kinase. Cloning and sequencing of the glpK gene and the primary structure of the enzyme.
Pettigrew, D. W., D.-P. Ma, C. A. Conrad, and J. R. Johnson.
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J Bacteriol. 1996;178:2846-2852
A single amino acid change in Escherichia coli glycerol kinase abolishes glucose control of glycerol utilization in vivo.
Pettigrew, D. W., W. Z. Liu, C. Holmes, N. D. Meadow, and S. Roseman.
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J Bacteriol. 1978;133(3):1358-67.
Permease-specific mutations in Salmonella typhimurium and Escherichia coli that release the glycerol, maltose, melibiose, and lactose transport systems from regulation by the phosphoenolpyruvate:sugar phosphotransferase system.
Saier MH Jr, Straud H, Massman LS, Judice JJ, Newman MJ, Feucht BU.
(
346569
)
|
J Bacteriol. 1966;92(4):1083-9.
Location of the maltose A and B loci on the genetic map of Escherichia coli.
Schwartz M.
(
5332865
)
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Mol Gen Genet. 1984;193(2):376-8.
Characterization of a glpK transducing phage.
Conrad CA, Stearns GW 3rd, Prater WE, Rheimer JA, Johnson JR.
(
6319974
)
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| Sequences |
Amino acid
FASTA format
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0001 MTEKKYIVAL DQGTTSSRAV VMDHDANIIS VSQREFEQIY PKPGWVEHDP MEIWATQSST LVEVLAKADI
0071 SSDQIAAIGI TNQRETTIVW EKETGKPIYN AIVWQCRRTA EICEHLKRDG LEDYIRSNTG LVIDPYFSGT
0141 KVKWILDHVE GSRERARRGE LLFGTVDTWL IWKMTQGRVH VTDYTNASRT MLFNIHTLDW DDKMLEVLDI
0211 PREMLPEVRR SSEVYGQTNI GGKGGTRIPI SGIAGDQQAA LFGQLCVKEG MAKNTYGTGC FMLMNTGEKA
0281 VKSENGLLTT IACGPTGEVN YALEGAVFMA GASIQWLRDE MKLINDAYDS EYFATKVQNT NGVYVVPAFT
0351 GLGAPYWDPY ARGAIFGLTR GVNANHIIRA TLESIAYQTR DVLEAMQADS GIRLHALRVD GGAVANNFLM
0421 QFQSDILGTR VERPEVREVT ALGAAYLAGL AVGFWQNLDE LQEKAVIERE FRPGIETTER NYRYAGWKKA
0491 VKRAMAWEEH DE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 cgccatttgc cttgcgatat ctgtgttgtg
-070 gaagaaaagg aaaccacaac tccttcagaa caaaaagctt cgctgtaata tgactacggg acaattaaac
0001 atgactgaaa aaaaatatat cgttgcgctc gaccagggca ccaccagctc ccgcgcggtc gtaatggatc
0071 acgatgccaa tatcattagc gtgtcgcagc gcgaatttga gcaaatctac ccaaaaccag gttgggtaga
0141 acacgaccca atggaaatct gggccaccca aagctccacg ctggtagaag tgctggcgaa agccgatatc
0211 agttccgatc aaattgcagc tatcggtatt acgaaccagc gtgaaaccac tattgtctgg gaaaaagaaa
0281 ccggcaagcc tatctataac gccattgtct ggcagtgccg tcgtaccgca gaaatctgcg agcatttaaa
0351 acgtgacggt ttagaagatt atatccgcag caataccggt ctggtgattg acccgtactt ttctggcacc
0421 aaagtgaagt ggatcctcga ccatgtggaa ggctctcgcg agcgtgcacg tcgtggtgaa ttgctgtttg
0491 gtacggttga tacgtggctt atctggaaaa tgactcaggg ccgtgtccat gtgaccgatt acaccaacgc
0561 ctctcgtacc atgttgttca acatccatac cctggactgg gacgacaaaa tgctggaagt gctggatatt
0631 ccgcgcgaga tgctgccaga agtgcgtcgt tcttccgaag tatacggtca gactaacatt ggcggcaaag
0701 gcggcacgcg tattccaatc tccgggatcg ccggtgacca gcaggccgcg ctgtttggtc agttgtgcgt
0771 gaaagaaggg atggcgaaga acacctatgg cactggctgc tttatgctga tgaacactgg cgagaaagcg
0841 gtgaaatcag aaaacggcct gctgaccacc atcgcctgcg gcccgactgg cgaagtgaac tatgcgttgg
0911 aaggtgcggt gtttatggca ggcgcatcca ttcagtggct gcgcgatgaa atgaagttga ttaacgacgc
0981 ctacgattcc gaatatttcg ccaccaaagt gcaaaacacc aatggtgtgt atgtggttcc ggcatttacc
1051 gggctgggtg cgccgtactg ggacccgtat gcgcgcgggg cgattttcgg tctgactcgt ggggtgaacg
1121 ctaaccacat tatacgcgcg acgctggagt ctattgctta tcagacgcgt gacgtgctgg aagcgatgca
1191 ggccgactct ggtatccgtc tgcacgccct gcgcgtggat ggtggcgcag tagcaaacaa tttcctgatg
1261 cagttccagt ccgatattct cggcacccgc gttgagcgcc cggaagtgcg cgaagtcacc gcattgggtg
1331 cggcctatct cgcaggcctg gcggttggct tctggcagaa cctcgacgag ctgcaagaga aagcggtgat
1401 tgagcgcgag ttccgtccag gcatcgaaac cactgagcgt aattaccgtt acgcaggctg gaaaaaagcg
1471 gttaaacgcg cgatggcgtg ggaagaacac gacgaataat gtaaatgccg aatgaagcgt ttatgccgca
1541 tccggtagtc ccgaaacgtg cgggggcaac cccgcacaca tcaataatcc ctcccttccc ctgtgctac
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