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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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OCR07
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
rhaD
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Related strains
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Strains Search
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Search strains by gene name:
rhaD
Search strains by all related name:
rhaD b3902 ECK3895 JW3873
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General information (Go to Linear View: )
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Gene Name
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rhaD
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Alternative name
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b3902,ECK3895,JW3873
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Location, Length
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4,091,471 - 4,092,295
(
-
)
;
88.18
min
;
825
(bp) ,
274
(aa)
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Product
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rhamnulose-1-phosphate aldolase
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Operon Name
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rhaBAD
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Note
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rhamnulose-phosphate aldolase; GO_process: GO:0016052 - carbohydrate catabolic process
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Function
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enzyme; Degradation of small molecules: Carbon compounds
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Gene Ontology
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GO:0005737
;
cytoplasm ( rhaD )
GO:0008270
;
zinc ion binding ( rhaD )
GO:0008994
;
rhamnulose-1-phosphate aldolase activity ( rhaD )
GO:0016829
;
lyase activity ( rhaD )
GO:0019299
;
rhamnose metabolic process ( rhaD )
GO:0046872
;
metal ion binding ( rhaD )
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PID
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1790336
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EC number
(KEGG Pathway)
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4.1.2.19
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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RHAD_ECOLI
(
P32169
)
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- Protein name
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Rhamnulose-1-phosphate aldolase
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- Synonyms
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EC 4.1.2.19
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- Gene name
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Name=rhaD; Synonyms=rhuA; OrderedLocusNames=b3902;
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- Comments
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FUNCTION:Catalyzes the reversible cleavage of L-rhamnulose-1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde.
CATALYTIC ACTIVITY:L-rhamnulose 1-phosphate = glycerone phosphate + (S)-lactaldehyde.
COFACTOR:Binds 1 zinc ion per subunit.
PATHWAY:Metabolism of L-rhamnose; third step.
SUBUNIT:Homotetramer.
SUBCELLULAR LOCATION:Cytoplasmic.
SIMILARITY:Belongs to the aldolase class II family. RhaD subfamily.
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Entry name(Acc.no)
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RHAD_ECOL6
(
Q8FBE1
)
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- Protein name
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Rhamnulose-1-phosphate aldolase
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- Synonyms
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EC 4.1.2.19
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- Gene name
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Name=rhaD; OrderedLocusNames=c4851;
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- Comments
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FUNCTION:Catalyzes the reversible cleavage of L-rhamnulose-1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde (By similarity).
CATALYTIC ACTIVITY:L-rhamnulose 1-phosphate = glycerone phosphate + (S)-lactaldehyde.
COFACTOR:Binds 1 zinc ion per subunit (By similarity).
PATHWAY:Metabolism of L-rhamnose; third step.
SUBUNIT:Homotetramer (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the aldolase class II family. RhaD subfamily.
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Entry name(Acc.no)
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RHAD_ECO57
(
Q8X8A1
)
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- Protein name
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Rhamnulose-1-phosphate aldolase
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- Synonyms
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EC 4.1.2.19
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- Gene name
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Name=rhaD; OrderedLocusNames=z5446, ECs4829;
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- Comments
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FUNCTION:Catalyzes the reversible cleavage of L-rhamnulose-1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde (By similarity).
CATALYTIC ACTIVITY:L-rhamnulose 1-phosphate = glycerone phosphate + (S)-lactaldehyde.
COFACTOR:Binds 1 zinc ion per subunit (By similarity).
PATHWAY:Metabolism of L-rhamnose; third step.
SUBUNIT:Homotetramer (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the aldolase class II family. RhaD subfamily.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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rhaD
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, L-Rhamnulose-1-Phosphate Aldolase From Escherichia Coli
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Rhamnulose-1-phosphate aldolase
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PSI-BLAST
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Rhamnulose-1-phosphate aldolase
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nr
(database updated :
2007.02.20
)
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BLAST
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rhamnulose-1-phosphate aldolase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
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MMBR References
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Nucleic Acids Res. 1993;21:3391-3398
Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes.
Plunkett, G., V. D. Burland, D. L. Daniels, and F. R. Blattner.
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J Mol Biol. 1987;196(4):789-99.
Positive regulation of the Escherichia coli L-rhamnose operon is mediated by the products of tandemly repeated regulatory genes.
Tobin JF, Schleif RF.
(
3316663
)
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| Sequences |
Amino acid
FASTA format
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0001 MQNITQSWFV QGMIKATTDA WLKGWDERNG GNLTLRLDDA DIAPYHDNFH QQPRYIPLSQ PMPLLANTPF
0071 IVTGSGKFFR NVQLDPAANL GIVKVDSDGA GYHILWGLTN EAVPTSELPA HFLSHCERIK ATNGKDRVIM
0141 HCHATNLIAL TYVLENDTAV FTRQLWEGST ECLVVFPDGV GILPWMVPGT DEIGQATAQE MQKHSLVLWP
0211 FHGVFGSGPT LDETFGLIDT AEKSAQVLVK VYSMGGMKQT ISREELIALG KRFGVTPLAS ALAL
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 cagcgtcgca tcaggcattg attgccggat
-070 gcggcgcgag cgccttatcc ggcctacggg tcgtgcatcc gacaacaccg aatttacagg aacacagaac
0001 atgcaaaaca ttactcagtc ctggtttgtc cagggaatga tcaaagccac caccgacgcc tggctgaaag
0071 gctgggatga gcgcaacggc ggcaacctga cgctacgcct ggatgacgcc gatatcgcac catatcacga
0141 caatttccac caacaaccgc gctatatccc gctcagccag cccatgcctt tactggcaaa tacaccgttt
0211 attgtcaccg gctcgggcaa attcttccgt aacgtccagc ttgatcctgc ggctaactta ggcatcgtaa
0281 aagtcgacag cgacggcgcg ggctaccaca ttctttgggg gttaaccaac gaagccgtcc ccacttccga
0351 acttccggct cacttccttt cccactgcga gcgcattaaa gccaccaacg gcaaagatcg ggtgatcatg
0421 cactgccacg ccaccaacct gatcgccctc acctatgtac ttgaaaacga caccgcggtc ttcactcgcc
0491 aactgtggga aggcagcacc gagtgtctgg tggtattccc ggatggcgtt ggcattttgc cgtggatggt
0561 gcccggcacg gacgaaatcg gccaggcgac cgcacaagag atgcaaaaac attcgctggt gttgtggccc
0631 ttccacggcg tcttcggcag cggaccgacg ctggatgaaa ccttcggttt aatcgacacc gcagaaaaat
0701 cagcacaagt attagtgaag gtttattcga tgggcggcat gaaacagacc atcagccgtg aagagttgat
0771 agcgctcggc aagcgtttcg gcgttacgcc actcgccagt gcgctggcgc tgtaaggagc aaacatgatc
0841 cgcaaagcct ttgtcatgca ggtaaacccc gacgcccacg aagagtatca gcgtcggcat aatcccatct
0911 ggccagaact ggaag
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