MG1655
W3110
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Gene Report : hemG
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
DNA Res. 1995;2(1):1-8.
Cloning and identification of the hemG gene encoding protoporphyrinogen oxidase (PPO) of Escherichia coli K-12.
Nishimura K, Nakayashiki T, Inokuchi H. ( 7788523 )
     Deletion OCR05 (D)  ,  OCR05-12-1 (D)  ,  OCR05-13 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  hemG
Related strains
     Strains Search Search strains by gene name:  hemG   Search strains by all related name:  hemG b3850 ECK3842 JW3827 o181 yihB

General information  (Go to Linear View:)
 Gene Name hemG  
 Alternative name b3850,ECK3842,JW3827,o181,yihB  
 Location, Length 4,032,631 - 4,033,176 (  +  ) ;   86.92 min ; 546 (bp) ,   181 (aa) Go to Linear View
 Product protoporphyrin oxidase, flavoprotein  
 Operon Name pepQ-yigZ-trkH-hemG  
 Note protoporphyrin oxidase  
 Function enzyme; Biosynthesis of cofactors, carriers: Heme, porphyrin  
 Gene Ontology GO:0004729 ; protoporphyrinogen oxidase activity ( hemG )
GO:0006118 ; electron transport ( hemG )
GO:0006779 ; porphyrin biosynthetic process ( hemG )
GO:0010181 ; FMN binding ( hemG )
GO:0016491 ; oxidoreductase activity ( hemG )
 PID 1790285  
 EC number
  (KEGG Pathway)   		1.3.3.4  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene hemG ( EG11485 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) HEMG_ECOLI ( P27863 )
    -  Protein name Protoporphyrinogen oxidase  
    -  Synonyms EC 1.3.3.4, PPO  
    -  Gene name Name=hemG; OrderedLocusNames=b3850, c4797, z5372, ECs4778;  
    -  Comments
  • FUNCTION:Catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Does not use oxygen directly. Is coupled to the respiratory chain.
  • CATALYTIC ACTIVITY:Protoporphyrinogen-IX + 1.5 O(2) = protoporphyrin-IX + 3 H(2)O.
  • PATHWAY:Porphyrin biosynthesis; penultimate step.
  • SUBUNIT:Belongs to a multi-protein complex.
  • SIMILARITY:Contains 1 flavodoxin-like domain.
  • CAUTION:Ref.2 sequence differs from that shown due to frameshifts.  

    •  Linear View (Whole Mode)
    View Location
       4020.0  –  4045.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP hemG (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain , Crystal Structure Of Oxidized Flavodoxin From A Red Alga Chondrus crispus Refined At 1.8 Angstroms Resolution: Description Of The Flavin Mononucleotide Binding Site  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Protoporphyrinogen oxidase (PPO)  
             PSI-BLAST Protoporphyrinogen oxidase
        nr     (database updated : 2007.02.20 )
             BLAST protoporphyrinogen oxidase [Escherichia coli O157:H7 EDL933]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    FLAVODOXIN     

    Other Cross-Reference
        COG COG4635CH 
        EcoCyc hemG 

    MMBR References
    DNA Res. 1995;2(1):1-8.
    Cloning and identification of the hemG gene encoding protoporphyrinogen oxidase (PPO) of Escherichia coli K-12.
    Nishimura K, Nakayashiki T, Inokuchi H. ( 7788523 )
    Can J Microbiol. 1993;39(12):1155-61.
    Nucleotide sequence of the hemG gene involved in the protoporphyrinogen oxidase activity of Escherichia coli K12.
    Sasarman A, Letowski J, Czaika G, Ramirez V, Nead MA, Jacobs JM, Morais R. ( 7916647 )
    J Gen Microbiol. 1979;113(2):297-303.
    Mapping of a new hem gene in Escherichia coli K12.
    Sasarman A, Chartrand P, Lavoie M, Tardif D, Proschek R, Lapointe C. ( 390093 )
    Proc Natl Acad Sci U S A. 1996;93:2459-2463
    Non-iron porphyrins cause tumbling to blue light by an Escherichia coli mutant defective in hemG.
    Yang, H., A. Sasarman, H. Inokuchi, and J. Adler.

    Sequences
    Amino acid
    FASTA format 
    0001 MKTLILFSTR DGQTREIASY LASELKELGI QADVANVHRI EEPQWENYDR VVIGASIRYG HYHSAFQEFV 
0071 KKHATRLNSM PSAFYSVNLV ARKPEKRTPQ TNSYARKFLM NSQWRPDRCA VIAGALRYPR YRWYDRFMIK 
0141 LIMKMSGGET DTRKEVVYTD WEQVANFARE IAHLTDKPTL K
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             AATGGATCCT GATTGCCAAC ATGCTGTTTG 
-070 GTCGTCTCGA GGTCTTTACA TTGCTGGTGC TCTTTACCCC GACTTTCTGG CGTGAATGAT GGAGTAATAC 
0001 gtgaaaacat taattctttt ctcaacaagg gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg 
0071 aactgaaaga actggggatc caggcggatg tcgccaatgt gcaccgcatt gaagaaccac agtgggaaaa 
0141 ctatgaccgt gtggtcattg gtgcttctat tcgctatggt cactaccatt cagcgttcca ggaatttgtc 
0211 aaaaaacatg cgacgcggct gaattcgatg ccgagcgcct tttactccgt gaatctggtg gcgcgcaaac 
0281 cggagaagcg tactccacag accaacagct acgcgcgcaa gtttctgatg aactcgcaat ggcgtcccga 
0351 tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt tatcgctggt acgaccgttt tatgatcaag 
0421 ctgattatga agatgtcagg cggtgaaacg gatacgcgca aagaagttgt ctataccgat tgggagcagg 
0491 tggcgaattt cgcccgagaa atcgcccatt taaccgacaa accgacgctg aaataaGCAT AAAGAATAAA 
0561 AAATGCGCGG TCAGAAAATT ATTTTAAATT TCCTCTTGTC AGGCCGGAAT AACTCCCTAT AATGCGCCAC 
0631 CACTGACACG GAACAA