MG1655
W3110
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Gene Report : rbsA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Protein Sci. 1996;5(6):1093-9.
The proteins encoded by the rbs operon of Escherichia coli: I. Overproduction, purification, characterization, and functional analysis of RbsA.
Barroga CF, Zhang H, Wajih N, Bouyer JH, Hermodson MA. ( 8762140 )
     Deletion OCR01-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  rbsA
Related strains
     Strains Search Search strains by gene name:  rbsA   Search strains by all related name:  rbsA b3749 ECK3743 JW3728 o501 rbsP rbsT

General information  (Go to Linear View:)
 Gene Name rbsA  
 Alternative name b3749,ECK3743,JW3728,o501,rbsP,rbsT  
 Location, Length 3,931,801 - 3,933,306 (  +  ) ;   84.74 min ; 1506 (bp) ,   501 (aa) Go to Linear View
 Product fused D-ribose transporter subunits of ABC superfamily: ATP-binding components  
 Operon Name rbsDACBKR  
 Note ATP-binding component of D-ribose high-affinity transport system; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0000166 ; nucleotide binding ( rbsA )
GO:0005351 ; sugar:hydrogen ion symporter activity ( rbsA )
GO:0005515 ; protein binding ( rbsA )
GO:0005524 ; ATP binding ( rbsA )
GO:0005886 ; plasma membrane ( rbsA )
GO:0006810 ; transport ( rbsA )
GO:0008643 ; carbohydrate transport ( rbsA )
GO:0015407 ; monosaccharide-transporting ATPase activity ( rbsA )
GO:0016020 ; membrane ( rbsA )
GO:0016787 ; hydrolase activity ( rbsA )
GO:0016887 ; ATPase activity ( rbsA )
GO:0017111 ; nucleoside-triphosphatase activity ( rbsA )
 PID 1790190  
 EC number
  (KEGG Pathway)   		 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene rbsA ( EG10814 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) RBSB_ECOLI ( P02925 )
    -  Protein name D-ribose-binding periplasmic protein precursor.  
    -  Synonyms  
    -  Gene name Name=rbsB; Synonyms=rbsP, prlB; OrderedLocusNames=b3751;  
    -  Comments
  • FUNCTION:Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis.
  • SUBCELLULAR LOCATION:Periplasmic.
  • SIMILARITY:Belongs to the bacterial extracellular solute-binding protein family 2.  
    •   botton Entry name(Acc.no) RBSA_ECOLI ( P04983 )
        -  Protein name Ribose transport ATP-binding protein rbsA.  
        -  Synonyms  
        -  Gene name Name=rbsA; OrderedLocusNames=b3749;  
        -  Comments
  • FUNCTION:Part of the binding-protein-dependent transport system for ribose. Probably responsible for energy coupling to the transport system.
  • SUBCELLULAR LOCATION:Inner membrane-associated.
  • DOMAIN:Composed of two homologous domains.
  • SIMILARITY:Belongs to the ABC transporter family. MglA subfamily.  
    •   botton Entry name(Acc.no) Q8FBS3 ( Q8FBS3 )
        -  Protein name Ribose transport ATP-binding protein rbsA.  
        -  Synonyms  
        -  Gene name Name=rbsA; OrderedLocusNames=c4677;  
        -  Comments
  • SIMILARITY:Belongs to the ABC transporter family.  
    •   botton Entry name(Acc.no) Q8XAW7 ( Q8XAW7 )
        -  Protein name ATP-binding component of D-ribose high-affinity transport system.  
        -  Synonyms  
        -  Gene name Name=rbsA; OrderedLocusNames=z5250;  
        -  Comments
  • SIMILARITY:Belongs to the ABC transporter family.  

    •  Linear View (Whole Mode)
    View Location
       3920.0  –  3945.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP rbsA (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Rnase-L Inhibitor  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Ribose import ATP-binding protein rbsA  
             PSI-BLAST Ribose import ATP-binding protein rbsA
        nr     (database updated : 2007.02.20 )
             BLAST fused D-ribose transporter subunits of ABC superfamily: ATP-binding components [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    ATP_GTP_A    ABC_TRANSPORTER     

    Other Cross-Reference
        COG COG1129G 
        EcoCyc rbsA 

    MMBR References
    J Bacteriol. 1984;158(2):674-82.
    Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12.
    Iida A, Harayama S, Iino T, Hazelbauer GL. ( 6327617 )
    Protein Sci. 1996;5:1093-1099
    The proteins encoded by the rbs operon of Escherichia coli: I. Overproduction, purification, characterization, and functional analysis of RbsA.
    Barroga, C. F., H. Zhang, N. Wajih, J. H. Bouyer, and M. A. Hermodson.
    J Bacteriol. 1984;158(2):665-73.
    D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport.
    Lopilato JE, Garwin JL, Emr SD, Silhavy TJ, Beckwith JR. ( 6327616 )
    J Biol Chem. 1986;261:7652-7658
    The nucleotide sequences of the rbsD, rbsA and rbsC genes of Escherichia coli K12.
    Bell, A. W., S. D. Buckel, J. M. Groarke, J. N. Hope, D. H. Kingsley, and A. Hermodsen.

    Sequences
    Amino acid
    FASTA format 
    0001 MEALLQLKGI DKAFPGVKAL SGAALNVYPG RVMALVGENG AGKSTMMKVL TGIYTRDAGT LLWLGKETTF 
0071 TGPKSSQEAG IGIIHQELNL IPQLTIAENI FLGREFVNRF GKIDWKTMYA EADKLLAKLN LRFKSDKLVG 
0141 DLSIGDQQMV EIAKVLSFES KVIIMDEPTD ALTDTETESL FRVIRELKSQ GRGIVYISHR MKEIFEICDD 
0211 VTVFRDGQFI AEREVASLTE DSLIEMMVGR KLEDQYPHLD KAPGDIRLKV DNLCGPGVND VSFTLRKGEI 
0281 LGVSGLMGAG RTELMKVLYG ALPRTSGYVT LDGHEVVTRS PQDGLANGIV YISEDRKRDG LVLGMSVKEN 
0351 MSLTALRYFS RAGGSLKHAD EQQAVSDFIR LFNVKTPSME QAIGLLSGGN QQKVAIARGL MTRPKVLILD 
0421 EPTRGVDVGA KKEIYQLINQ FKADGLSIIL VSSEMPEVLG MSDRIIVMHE GHLSGEFTRE QATQEVLMAA 
0491 AVGKLNRVNQ E
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             AAACAACAAA CCGCAGAAAG TCAGGCGGTA 
-070 ATTCGCAGCG GAGAATGTTC TCCGTATGCG AATATCATTC TCTGTGCTGG CGTGACGTTC TGAGGCCGTC 
0001 atggaagcat tacttcagct taaaggcatc gataaagcct tcccgggcgt aaaagccctc tcgggcgcag 
0071 cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg cgaaaacggc gcgggtaaat ccaccatgat 
0141 gaaagtgctt actggcatct atactcgcga tgccggtacg cttttatggc tggggaaaga aacgacattt 
0211 accgggccaa aatcttccca ggaagccggg attgggatta tccatcagga actgaacctg atcccgcagt 
0281 tgaccattgc cgaaaacatt ttcctcggtc gtgagtttgt taatcgcttt ggcaaaattg actggaaaac 
0351 catgtatgcc gaagcggata aattgctggc taaacttaac ctgcgcttta aaagcgacaa gctggtgggc 
0421 gatctttcca tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag ctttgagtcg aaagtcatca 
0491 ttatggatga accgaccgat gcgctgaccg ataccgaaac cgaatccctg ttccgcgtca tccgcgagct 
0561 gaaatcgcaa ggccgcggta ttgtctatat ctcccaccgc atgaaagaaa tcttcgagat ttgcgatgac 
0631 gttaccgttt ttcgtgatgg gcaatttatt gctgagcgcg aagtggcatc actgaccgaa gattcgctga 
0701 ttgagatgat ggtgggtcgc aagctggaag atcaatatcc gcacctggac aaagcgccgg gagatatccg 
0771 cctgaaagtc gataatctct gcggacctgg cgttaacgat gtctctttta ctttacgcaa aggcgaaatt 
0841 cttggcgtct ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt gctctacggc gcactaccgc 
0911 gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt tacccgttca ccgcaggatg gcctggcaaa 
0981 cggcattgtg tatatctccg aagaccgtaa acgtgacggt ttagtgttgg gcatgtcagt aaaagagaac 
1051 atgtcgctga cagcgctgcg ctacttcagc cgcgctggcg gcagtttgaa gcatgccgat gaacagcagg 
1121 ctgtgagtga tttcattcgt ctgtttaatg tgaaaactcc gtcgatggaa caggcaattg gtctgctttc 
1191 cggtggcaat cagcaaaaag tggcgattgc ccgcggtctg atgacacgcc ccaaagtgtt gatccttgat 
1261 gaacctaccc gtggcgtaga tgtcggcgcg aaaaaagaga tctatcaact gattaaccag ttcaaagccg 
1331 atggcttgag catcattctg gtgtcatcgg agatgccaga agtattaggc atgagcgatc gcatcatcgt 
1401 catgcatgaa gggcatctca gcggggaatt tactcgtgag caggccaccc aggaagtgtt aatggctgcc 
1471 gctgtgggca agcttaatcg cgtgaatcag gagtaaAAAA ATGACAACCC AGACTGTCTC TGGTCGCCGT 
1541 TATTTCACGA AAGCGTGGCT GATGGAGCAG AAATCGCTTA TCGCTCTGCT GGTGCTGATC GCGATT