MG1655
W3110
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Gene Report : pstA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCL02 (D)  ,  DE(OC3)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  pstA
Related strains
     Strains Search Search strains by gene name:  pstA   Search strains by all related name:  pstA b3726 ECK3719 JW3704 phoR2b phoT R2pho

General information  (Go to Linear View:)
 Gene Name pstA  
 Alternative name b3726,ECK3719,JW3704,phoR2b,phoT,R2pho  
 Location, Length 3,906,572 - 3,907,462 (  -  ) ;   84.20 min ; 891 (bp) ,   296 (aa) Go to Linear View
 Product phosphate transporter subunit  
 Operon Name pstSCAB-phoU  
 Note high-affinity phosphate-specific transport system; membrane component of ABC superfamily; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006793 - phosphorus metabolic process  
 Function transport; Transport of small molecules: Anions  
 Gene Ontology GO:0005215 ; transporter activity ( pstA )
GO:0005315 ; inorganic phosphate transmembrane transporter activity ( pstA )
GO:0005886 ; plasma membrane ( pstA )
GO:0006810 ; transport ( pstA )
GO:0006817 ; phosphate transport ( pstA )
GO:0009276 ; 1-2nm peptidoglycan-based cell wall ( pstA )
GO:0015114 ; phosphate transmembrane transporter activity ( pstA )
GO:0016020 ; membrane ( pstA )
GO:0016021 ; integral to membrane ( pstA )
 PID 1790163  
 EC number
  (KEGG Pathway)   		 
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) PSTA_ECOLI ( P07654 )
    -  Protein name Phosphate transport system permease protein pstA.  
    -  Synonyms  
    -  Gene name Name=pstA; Synonyms=phoT; OrderedLocusNames=b3726;  
    -  Comments
  • FUNCTION:Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.
  • SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane.
  • SIMILARITY:Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.  
    •   botton Entry name(Acc.no) PSTB_ECOLI ( P07655 )
        -  Protein name Phosphate import ATP-binding protein pstB  
        -  Synonyms EC 3.6.3.27, Phosphate- transporting ATPase, ABC phosphate transporter  
        -  Gene name OrderedLocusNames=b3725, c4649, z5216, ECs4661, SF3730, S4042;  
        -  Comments
  • FUNCTION:Part of the ABC transporter complex pstSACB involved in phosphate import. Responsible for energy coupling to the transport system.
  • CATALYTIC ACTIVITY:ATP + H(2)O + phosphate(Out) = ADP + phosphate + phosphate(In).
  • SUBUNIT:The complex is composed of two ATP-binding proteins (pstB), two transmembrane proteins (pstC and pstA) and a solute-binding protein (pstS) (Probable).
  • SUBCELLULAR LOCATION:Inner membrane-associated.
  • SIMILARITY:Belongs to the ABC transporter family. Phosphate importer (TC 3.A.1.7) subfamily.  
    •   botton Entry name(Acc.no) Q8FBT6 ( Q8FBT6 )
        -  Protein name Phosphate transport system permease protein pstA.  
        -  Synonyms  
        -  Gene name Name=pstA; OrderedLocusNames=c4651;  
        -  Comments
  • FUNCTION:Part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane (By similarity).
  • SUBCELLULAR LOCATION:Integral membrane protein (By similarity).
  • SIMILARITY:Belongs to the binding-protein-dependent transport system permease family.  
    •   botton Entry name(Acc.no) Q8XB05 ( Q8XB05 )
        -  Protein name High-affinity phosphate-specific transport system.  
        -  Synonyms  
        -  Gene name Name=pstA; OrderedLocusNames=z5217;  
        -  Comments
  • FUNCTION:Part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane (By similarity).
  • SUBCELLULAR LOCATION:Integral membrane protein (By similarity).
  • SIMILARITY:Belongs to the binding-protein-dependent transport system permease family.  

    •  Linear View (Whole Mode)
    View Location
       3895.0  –  3920.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP pstA (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
            PSI-BLAST  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Phosphate transport system permease protein pstA  
             PSI-BLAST Phosphate transport system permease protein pstA
        nr     (database updated : 2007.02.20 )
             BLAST phosphate transporter subunit [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    BPD_TRANSP_INN_MEMBR     

    Other Cross-Reference
        COG COG0581P 
        EcoCyc pstA 

    MMBR References
    In B. P. Rosen, and S. Silver (ed.), Ion transport in prokaryotes. Academic Press, Inc., New York, N.Y. 1987:205-246
    Phosphate transport in prokaryotes.
    Rosenberg, H.
    J Bacteriol. 1993;175:6797-6809
    Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon.
    Steed, P. M., and B. L. Wanner.
    J Bacteriol. 1985;161(1):189-98.
    Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships.
    Surin BP, Rosenberg H, Cox GB. ( 3881386 )
    J Bacteriol. 1982;152(2):692-701.
    Cloning of and complementation tests with alkaline phosphatase regulatory genes (phoS and phoT) of Escherichia coli.
    Amemura M, Shinagawa H, Makino K, Otsuji N, Nakata A. ( 6290447 )
    J Mol Biol. 1985;184(2):241-50.
    Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli.
    Amemura M, Makino K, Shinagawa H, Kobayashi A, Nakata A. ( 2993631 )

    Sequences
    Amino acid
    FASTA format 
    0001 MAMVEMQTTA ALAESRRKMQ ARRRLKNRIA LTLSMATMAF GLFWLIWILM STITRGIDGM SLALFTEMTP 
0071 PPNTEGGGLA NALAGSGLLI LWATVFGTPL GIMAGIYLAE YGRKSWLAEV IRFINDILLS APSIVVGLFV 
0141 YTIVVAQMEH FSGWAGVIAL ALLQVPIVIR TTENMLKLVP YSLREAAYAL GTPKWKMISA ITLKASVSGI 
0211 MTGILLAIAR IAGETAPLLF TALSNQFWST DMMQPIANLP VTIFKFAMSP FAEWQQLAWA GVLIITLCVL 
0281 LLNILARVVF AKNKHG
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             tgatggaact gggcctgatc ctgtttgtga 
-070 ttaccttcat cgtcctcgcc gcatcgaagt ttatgattat gcgcctggct aagaatgagg gggcacgcta 
0001 atggctatgg ttgaaatgca aaccactgcg gcgctggctg aatctcgccg caaaatgcag gcgcgtcgcc 
0071 gcctcaaaaa ccgtattgcg ctgacgctct cgatggcgac gatggccttc ggcctgttct ggctgatctg 
0141 gattttaatg tccaccatca ctcgcggtat cgacggtatg tcgctggcgc tgttcactga aatgacgccg 
0211 ccgcccaata cggaaggtgg tggtctggcg aacgctctgg cgggtagcgg gctgttaatt ttgtgggcca 
0281 cggtattcgg tacgccgctg ggcattatgg cggggattta tctggcggaa tatggtcgta aatcctggct 
0351 ggcagaagtg attcgcttca ttaacgacat tctgctctct gcgccgtcga ttgtggttgg tctgtttgtt 
0421 tacaccattg tggtggcgca gatggagcac ttctccggct gggcgggcgt gattgccctg gcgttgttgc 
0491 aggtgccgat tgttatccgc accaccgaga acatgctgaa actggtgccg tacagcctgc gtgaagcggc 
0561 ttatgcgctg ggtacaccga agtggaagat gatctctgcg attacgctga aagcgtcggt gtccgggatt 
0631 atgaccggta tcctgctggc gattgcccgt attgctggtg aaaccgcgcc gctgctgttt accgcgctct 
0701 ccaaccagtt ctggagcacg gacatgatgc agccgatcgc caacctgccg gtgacgatct ttaagtttgc 
0771 gatgagcccg tttgcggaat ggcagcaatt ggcctgggcc ggggtattga tcattaccct gtgcgtactg 
0841 ctgctgaaca ttctggcgcg cgttgttttt gcgaagaata aacacggttg atattgctga cacggttttc 
0911 ccctcaccct aaccctctcc ccagaggggc gaggggaccg accgagcgcc tttttgactc tgtacacggt 
0981 taacactttg c