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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCL06
(D)
,
LD3-12-1
(LD)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
ilvB
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Related strains
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Strains Search
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Search strains by gene name:
ilvB
Search strains by all related name:
ilvB b3671 ECK3662 JW3646
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General information (Go to Linear View: )
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Gene Name
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ilvB
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Alternative name
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b3671,ECK3662,JW3646
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Location, Length
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3,849,119 - 3,850,807
(
-
)
;
82.96
min
;
1689
(bp) ,
562
(aa)
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Product
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acetolactate synthase I, large subunit
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Operon Name
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ivbL-ilvBN-uhpABC
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Note
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acetolactate synthase I,valine-sensitive, large subunit
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Function
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enzyme; Amino acid biosynthesis: Isoleucine, Valine
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Gene Ontology
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GO:0000287
;
magnesium ion binding ( ilvB )
GO:0003824
;
catalytic activity ( ilvB )
GO:0003984
;
acetolactate synthase activity ( ilvB )
GO:0008652
;
amino acid biosynthetic process ( ilvB )
GO:0009082
;
branched chain family amino acid biosynthetic process ( ilvB )
GO:0016740
;
transferase activity ( ilvB )
GO:0030976
;
thiamin pyrophosphate binding ( ilvB )
GO:0046872
;
metal ion binding ( ilvB )
GO:0050660
;
FAD binding ( ilvB )
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PID
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1790104
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EC number
(KEGG Pathway)
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2.2.1.6
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
|
ILVB_ECOLI
(
P08142
)
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- Protein name
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Acetolactate synthase isozyme I large subunit
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- Synonyms
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EC 2.2.1.6, AHAS-I, Acetohydroxy-acid synthase I large subunit, ALS-I
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- Gene name
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Name=ilvB; OrderedLocusNames=b3671;
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- Comments
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CATALYTIC ACTIVITY:2 pyruvate = 2-acetolactate + CO(2).
COFACTOR:Binds 1 thiamine pyrophosphate and 1 magnesium ion per subunit (By similarity).
PATHWAY:Valine and isoleucine biosynthesis; first step.
SUBUNIT:Dimer of large and small chains.
MISCELLANEOUS:E.coli contains genes for 3 AHAS isozymes:ilvBN, ilvGM and ilvIH.
MISCELLANEOUS:Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry (By similarity).
SIMILARITY:Belongs to the TPP enzyme family.
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Entry name(Acc.no)
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Q8FBX2
(
Q8FBX2
)
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- Protein name
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Acetolactate synthase isozyme I large subunit
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- Synonyms
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EC 4.1.3.18
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- Gene name
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Name=ilvB; OrderedLocusNames=c4596;
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- Comments
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CATALYTIC ACTIVITY:2 pyruvate = 2-acetolactate + CO(2).
COFACTOR:Binds 1 thiamine pyrophosphate and 1 magnesium ion per subunit (By similarity).
PATHWAY:Valine and isoleucine biosynthesis; first step.
SIMILARITY:Belongs to the TPP enzyme family.
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Entry name(Acc.no)
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Q8XC23
(
Q8XC23
)
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- Protein name
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Acetolactate synthase I,valine-sensitive, large subunit.
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- Synonyms
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- Gene name
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Name=ilvB; OrderedLocusNames=z5165;
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- Comments
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CATALYTIC ACTIVITY:2 pyruvate = 2-acetolactate + CO(2).
COFACTOR:Binds 1 thiamine pyrophosphate and 1 magnesium ion per subunit (By similarity).
PATHWAY:Valine and isoleucine biosynthesis; first step.
SIMILARITY:Belongs to the TPP enzyme family.
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MMBR References
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Nucleic Acids Res. 1985;13:3979-3993
The ilvB locus of Escherichia coli K-12 is an operon encoding both subunits of acetohydroxy acid synthase I.
Friden, P., J. Donegan, J. Mullen, P. Tsui, M. Freundlich, L. Eoyang, R. Weber, and P. M. Silverman.
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Proc Natl Acad Sci U S A. 1982;79:6156-6160
Nucleotide sequence of the ilvB promoter-regulatory region: a biosynthetic operon controlled by attenuation and cyclic AMP.
Friden, P., T. Newman, and M. Freundlich.
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J Bacteriol. 1982;149(1):387-90.
Acetohydroxy acid synthase is a target for leucine containing peptide toxicity in Escherichia coli.
Gollop N, Tavori H, Barak Z.
(
7033214
)
|
Nucleic Acids Res. 1983;11:127-139
Nucleotide sequence of the ilvB multivalent attenuator region of Escherichia coli K-12.
Hauser, C. A., and G. W. Hatfield.
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J Bacteriol. 1983;155(3):1052-61.
Genetic control of the hexose phosphate transport system of Escherichia coli: mapping of deletion and insertion mutations in the uhp region.
Kadner RJ, Shattuck-Eidens DM.
(
6309737
)
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Genetics 1980;96:59-77
A new map location for the ilvB locus of Escherichia coli.
Newman, T., and M. Levinthal.
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Mol Gen Genet. 1982;186(3):378-84.
Cloning and expression of the ilvB gene of Escherichia coli K-12.
Newman T, Friden P, Sutton A, Freundlich M.
(
6181375
)
|
J Bacteriol. 1973;114(1):183-94.
Isoleucine and valine metabolism in Escherichia coli. XXI.Mutations affecting derepression and valine resistance.
Pledger, W.J., H.E. Umbarger.
(
4569402
)
|
J Bacteriol. 1965;89:654-60.
REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES.
Ramakrishnan T, Adelberg EA.
(
14273640
)
|
J Bacteriol. 1983;155(3):1062-70.
Molecular cloning of the uhp region and evidence for a positive activator for expression of the hexose phosphate transport system of Escherichia coli.
Shattuck-Eidens DM, Kadner RJ.
(
6350260
)
|
J Bacteriol. 1981;148(3):998-1001.
Valine-resistant Escherichia coli K-12 strains with mutations in the ilvB operon.
Sutton A, Newman T, Francis M, Freundlich M.
(
7031038
)
|
Curr. Top. Microbiol. Immunol. 1969;1:57-76.
Regulation of the biosynthesis of the branched-chain amino acids.
Umbarger, H. E.
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Nucleic Acids Res. 1985;13:3995-4010
The nucleotide sequence of the ilvBN operon of Escherichia coli: sequence homologies of the acetohydroxy acid synthase isozyme.
Wek, R. C., C. A. Hauser, and G. W. Hatfield.
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J Bacteriol. 1991;173(17):5258-9.
Locations of the lip, poxB, and ilvBN genes on the physical map of Escherichia coli.
Chang YY, Cronan JE Jr, Li SJ, Reed K, Vanden Boom T, Wang AY.
(
1832150
)
|
Mol Gen Genet. 1977;156(3):239-49.
Valine-sensitive acetohydroxy acid synthases in Escherichia coli K-12: unique regulation modulated by multiple genetic sites.
Davis EJ, Blatt JM, Henderson EK, Whittaker JJ, Jackson JH.
(
340906
)
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| Sequences |
Amino acid
FASTA format
|
0001 MASSGTTSTR KRFTGAEFIV HFLEQQGIKI VTGIPGGSIL PVYDALSQST QIRHILARHE QGAGFIAQGM
0071 ARTDGKPAVC MACSGPGATN LVTAIADARL DSIPLICITG QVPASMIGTD AFQEVDTYGI SIPITKHNYL
0141 VRHIEELPQV MSDAFRIAQS GRPGPVWIDI PKDVQTAVFE IETQPAMAEK AAAPAFSEES IRDAAAMINA
0211 AKRPVLYLGG GVINAPARVR ELAEKAQLPT TMTLMALGML PKAHPLSLGM LGMHGVRSTN YILQEADLLI
0281 VLGARFDDRA IGKTEQFCPN AKIIHVDIDR AELGKIKQPH VAIQADVDDV LAQLIPLVEA QPRAEWHQLV
0351 ADLQREFPCP IPKACDPLSH YGLINAVAAC VDDNAIITTD VGQHQMWTAQ AYPLNRPRQW LTSGGLGTMG
0421 FGLPAAIGAA LANPDRKVLC FSGDGSLMMN IQEMATASEN QLDVKIILMN NEALGLVHQQ QSLFYEQGVF
0491 AATYPGKINF MQIAAGFGLE TCDLNNEADP QASLQEIINR PGPALIHVRI DAEEKVYPMV PPGAANTEMV
0561 GE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 ggaacaacac acgattccaa aaccccgccg
-070 gcgcaaaccg ggcggggttt ttcgtttaag cacctcccgg aaagtcggcc cagaagaaaa ggactggagc
0001 atggcaagtt cgggcacaac atcgacgcgt aagcgcttta ccggcgcaga atttatcgtt catttcctgg
0071 aacagcaggg cattaagatt gtgacaggca ttccgggcgg ttctatcctg cctgtttacg atgccttaag
0141 ccaaagcacg caaatccgcc atattctggc ccgtcatgaa cagggcgcgg gctttatcgc tcagggaatg
0211 gcgcgcaccg acggtaaacc ggcggtctgt atggcctgta gcggaccggg tgcgactaac ctggtgaccg
0281 ccattgccga tgcgcggctg gactccatcc cgctgatttg catcactggt caggttcccg cctcgatgat
0351 cggcaccgac gccttccagg aagtggacac ctacggcatc tctatcccca tcaccaaaca caactatctg
0421 gtcagacata tcgaagaact cccgcaggtc atgagcgatg ccttccgcat tgcgcaatca ggccgcccag
0491 gcccggtgtg gatagacatt cctaaggatg tgcaaacggc agtttttgag attgaaacac agcccgctat
0561 ggcagaaaaa gccgccgccc ccgcctttag cgaagaaagc attcgtgacg cagcggcgat gattaacgct
0631 gccaaacgcc cggtgcttta tctgggcggc ggtgtgatca atgcgcccgc acgggtgcgt gaactggcgg
0701 agaaagcgca actgcctacc accatgactt taatggcgct gggcatgttg ccaaaagcgc atccgttgtc
0771 gctgggtatg ctggggatgc acggcgtgcg cagcaccaac tatattttgc aggaggcgga tttgttgata
0841 gtgctcggtg cgcgttttga tgaccgggcg attggcaaaa ccgagcagtt ctgtccgaat gccaaaatca
0911 ttcatgtcga tatcgaccgt gcagagctgg gtaaaatcaa gcagccgcac gtggcgattc aggcggatgt
0981 tgatgacgtg ctggcgcagt tgatcccgct ggtggaagcg caaccgcgtg cagagtggca ccagttggta
1051 gcggatttgc agcgtgagtt tccgtgtcca atcccgaaag cgtgcgatcc gttaagccat tacggcctga
1121 tcaacgccgt tgccgcctgt gtcgatgaca atgcaattat caccaccgac gttggtcagc atcagatgtg
1191 gaccgcgcaa gcttatccgc tcaatcgccc acgccagtgg ctgacctccg gtgggctggg cacgatgggt
1261 tttggcctgc ctgcggcgat tggcgctgcg ctggcgaacc cggatcgcaa agtgttgtgt ttctccggcg
1331 acggcagcct gatgatgaat attcaggaga tggcgaccgc cagtgaaaat cagctggatg tcaaaatcat
1401 tctgatgaac aacgaagcgc tggggctggt gcatcagcaa cagagtctgt tctacgagca aggcgttttt
1471 gccgccacct atccgggcaa aatcaacttt atgcagattg ccgccggatt cggcctcgaa acctgtgatt
1541 tgaataacga agccgatccg caggcttcat tgcaggaaat catcaatcgc cctggcccgg cgctgatcca
1611 tgtgcgcatt gatgccgaag aaaaagttta cccgatggtg ccgccaggtg cggcgaatac tgaaatggtg
1681 ggggaataag ccatgcaaaa cacaactcat gacaacgtaa ttctggagct caccgttcgc aaccatccgg
1751 gcgtaatgac ccacgtttgt ggcctttttg cccgccgcg
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