MG1655
W3110
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Gene Report : mtlA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL09 (D)  ,  OCL09-2 (D)  ,  OCL09-9 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  mtlA
Related strains
     Strains Search Search strains by gene name:  mtlA   Search strains by all related name:  mtlA b3599 ECK3588 JW3573 mtlC

General information  (Go to Linear View:)
 Gene Name mtlA  
 Alternative name b3599,ECK3588,JW3573,mtlC  
 Location, Length 3,770,304 - 3,772,217 (  +  ) ;   81.26 min ; 1914 (bp) ,   637 (aa) Go to Linear View
 Product fused mannitol-specific PTS enzymes: IIAcomponents/IIB components/IIC components  
 Operon Name mtlADR  
 Note PTS system, mannitol-specific enzyme IIABC components; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0005215 ; transporter activity ( mtlA )
GO:0005351 ; sugar:hydrogen ion symporter activity ( mtlA )
GO:0005515 ; protein binding ( mtlA )
GO:0005886 ; plasma membrane ( mtlA )
GO:0006810 ; transport ( mtlA )
GO:0008643 ; carbohydrate transport ( mtlA )
GO:0008982 ; protein-N(PI)-phosphohistidine-sugar phosphotransferase activity ( mtlA )
GO:0009401 ; phosphoenolpyruvate-dependent sugar phosphotransferase system ( mtlA )
GO:0016020 ; membrane ( mtlA )
GO:0016021 ; integral to membrane ( mtlA )
GO:0016740 ; transferase activity ( mtlA )
 PID 1790027  
 EC number
  (KEGG Pathway)   		2.7.1.69  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) PTMA_ECOLI ( P00550 )
    -  Protein name PTS system, mannitol-specific IIABC component  
    -  Synonyms EIIABC-Mtl, Mannitol- permease IIABC component, Phosphotransferase enzyme II, ABC component, EC 2.7.1.69, EII-Mtl  
    -  Gene name Name=mtlA; OrderedLocusNames=b3599;  
    -  Comments
  • FUNCTION:This is a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system. The IICD domains contain the sugar binding site and the transmembrane channel; the IIA domain contains the primary phosphorylation site (the donor is phospho-HPr); IIA transfers its phosphoryl group to the IIB domain which finally transfers it to the sugar.
  • CATALYTIC ACTIVITY:Protein N-phosphohistidine + sugar = protein histidine + sugar phosphate.
  • SUBUNIT:Homodimer.
  • SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane.
  • PTM:An intramolecular phosphotransfer takes places between His-554 and Cys-384.
  • SIMILARITY:Contains 1 PTS EIIA domain.
  • SIMILARITY:Contains 1 PTS EIIB domain.
  • SIMILARITY:Contains 1 PTS EIIC domain.  
    •   botton Entry name(Acc.no) Q8FCB8 ( Q8FCB8 )
        -  Protein name PTS system, mannitol-specific IIABC component.  
        -  Synonyms  
        -  Gene name Name=mtlA; OrderedLocusNames=c4416;  
        -  Comments
  • SIMILARITY:Contains 1 HTH tetR-type DNA-binding domain.  
    •   botton Entry name(Acc.no) Q8XDH1 ( Q8XDH1 )
        -  Protein name PTS system, mannitol-specific enzyme IIABC components.  
        -  Synonyms  
        -  Gene name Name=mtlA; OrderedLocusNames=z5023;  
        -  Comments
  • SIMILARITY:Contains 1 HTH gntR-type DNA-binding domain.  

    •  Linear View (Whole Mode)
    View Location
       3760.0  –  3785.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP mtlA (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Structure Of Iib Domain Of The Mannitol-Specific Permease Enzyme Ii  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST PTS system mannitol-specific EIICBA component  
             PSI-BLAST PTS system mannitol-specific EIICBA component
        nr     (database updated : 2007.02.20 )
             BLAST fused mannitol-specific PTS enzymes: IIA components/IIB components/IIC components [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    PTS_EIIA_2     

    Other Cross-Reference
        COG COG2213G  COG4668G 
        EcoCyc mtlA 

    MMBR References
    Mol Microbiol. 1990;4(11):2003-6.
    Corrected sequence of the mannitol (mtl) operon in Escherichia coli.
    Jiang W, Wu LF, Tomich J, Saier MH Jr, Niehaus WG. ( 1964486 )
    J Bacteriol. 1985;161(3):1156-61.
    Escherichia coli 6S RNA is not essential for growth or protein secretion.
    Lee CA, Fournier MJ, Beckwith J. ( 2579059 )
    Proc Natl Acad Sci U S A. 1981;78:7336-7340
    Plasmid-directed synthesis of enzymes required for D-mannitol transport and utilization in Escherichia coli.
    Lee, L. G., G. R. Jacobson, and M. H. Saier.
    Mol Microbiol. 1988;2(3):405-12.
    Nucleotide sequence of the mannitol (mtl) operon in Escherichia coli.
    Davis T, Yamada M, Elgort M, Saier MH Jr. ( 3135464 )

    Sequences
    Amino acid
    FASTA format 
    0001 MSSDIKIKVQ SFGRFLSNMV MPNIGAFIAW GIITALFIPT GWLPNETLAK LVGPMITYLL PLLIGYTGGK 
0071 LVGGERGGVV GAITTMGVIV GADMPMFLGS MIAGPLGGWC IKHFDRWVDG KIKSGFEMLV NNFSAGIIGM 
0141 ILAILAFLGI GPIVEALSKM LAAGVNFMVV HDMLPLASIF VEPAKILFLN NAINHGIFSP LGIQQSHELG 
0211 KSIFFLIEAN PGPGMGVLLA YMFFGRGSAK QSAGGAAIIH FLGGIHEIYF PYVLMNPRLI LAVILGGMTG 
0281 VFTLTILGGG LVSPASPGSI LAVLAMTPKG AYFANIAGVC AAMAVSFVVS AILLKTSKVK EEDDIEAATR 
0351 RMQDMKAESK GASPLSAGDV TNDLSHVRKI IVACDAGMGS SAMGAGVLRK KIQDAGLSQI SVTNSAINNL 
0421 PPDVDLVITH RDLTERAMRQ VPQAQHISLT NFLDSGLYTS LTERLVAAQR HTANEEKVKD SLKDSFDDSS 
0491 ANLFKLGAEN IFLGRKAATK EEAIRFAGEQ LVKGGYVEPE YVQAMLDREK LTPTYLGESI AVPHGTVEAK 
0561 DRVLKTGVVF CQYPEGVRFG EEEDDIARLV IGIAARNNEH IQVITSLTNA LDDESVIERL AHTTSVDEVL 
0631 ELLAGRK
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             TTCGCAGTAT CTACAAGGTC CGGCTACCTC 
-070 TGCCGCCACA TTAACAAAAA ACCTCGGGCT TCCAGCCTGC GCGACAGCAA ACATAAGAAG GGGTGTTTTT 
0001 atgtcatccg atattaagat caaagtgcaa agctttggtc gtttcctcag caacatggtg atgccaaata 
0071 tcggcgcgtt tatcgcgtgg ggtatcatca ccgcgttatt tattccaaca gggtggttac cgaacgagac 
0141 gctggcgaag ctggtcgggc cgatgatcac ttatctcctg ccgctgctga tcggttatac cggtggtaag 
0211 ctggtaggcg gcgaacgtgg cggcgtagtc ggtgccatca ccaccatggg cgttatcgtc ggcgcagaca 
0281 tgccgatgtt cctcggttct atgattgcag gtccgctggg cggctggtgc attaagcact tcgaccgctg 
0351 ggtagacggt aagatcaaat ccggttttga gatgctggtg aataacttct ccgcaggcat catcgggatg 
0421 atcctcgcta ttctggcatt cctcggcatt ggcccgattg ttgaagccct gtccaaaatg ctggctgcgg 
0491 gcgttaactt catggttgtc catgacatgc tgccgctggc gtctatcttt gttgaaccgg cgaaaatcct 
0561 gttcctcaac aacgccatta accacggtat cttctcgccg ctgggtattc agcagtccca tgaactgggt 
0631 aaatcaatct tcttcctgat tgaagctaac ccaggtccag gtatgggcgt gctgctggcg tacatgttct 
0701 ttggtcgtgg tagcgctaaa cagtctgcgg gcggtgcggc aatcatccac ttcctggggg gtatccacga 
0771 aatctacttc ccgtatgtgc tgatgaatcc gcgtctgatc ctcgcagtca tcctcggcgg tatgactggc 
0841 gtgttcacgc tgactatcct gggcggtggt ctggtttctc cggcatctcc gggttctatc cttgctgtac 
0911 tggcgatgac accaaaaggt gcttacttcg ctaacatcgc gggtgtgtgt gcggcgatgg ctgtctcctt 
0981 cgttgtctct gctattttgc tgaaaaccag caaagtgaaa gaagaagatg atattgaagc agcaactcgt 
1051 cgtatgcagg acatgaaagc tgagtctaaa ggcgcatctc cgctgtctgc tggcgatgtg actaacgacc 
1121 tgagccacgt acgtaaaatc atcgttgcct gtgacgccgg tatgggttcc agtgcgatgg gcgcaggcgt 
1191 tctgcgtaag aaaattcagg atgcaggtct gtcgcagatt tctgttacta acagcgcgat caacaacctg 
1261 ccgccagatg tggacctcgt catcactcac cgtgacctga ccgaacgcgc tatgcgccag gttccgcagg 
1331 cacagcatat ttcgctgacc aacttcctcg acagcggcct gtacaccagc ctgaccgaac gtctggttgc 
1401 tgcccaacgc cacacggcaa acgaagagaa agtaaaagac agcctgaaag acagctttga cgattccagt 
1471 gctaacctgt tcaagctagg cgcggagaac atcttcctcg gtcgcaaagc ggcaaccaaa gaagaagcga 
1541 ttcgttttgc tggcgagcag ctggtgaaag gcggttacgt tgagccggaa tacgttcagg cgatgctgga 
1611 tcgtgaaaaa ctgaccccga cttatctggg tgagtctatc gcggtgccac acggtacggt tgaagcgaaa 
1681 gatcgcgtac tgaaaacggg cgtcgtgttc tgccagtacc cggaaggcgt gcgcttcggt gaagaagaag 
1751 atgacattgc ccgtctggtg attggtattg ctgcccgtaa caacgagcac attcaggtta tcaccagcct 
1821 gaccaatgca ctggatgatg agtccgtcat cgagcgtctg gcacacacca ccagcgtgga tgaagtgctg 
1891 gaactgctgg caggtcgtaa gtaaTCCAAT CCCACCCTCT CCACATGGAG AAGGTGGGGT TAATTGCCTG 
1961 ATGCGCTACG CTTATCAGGC CTACAGGATG CATCACAATT TGTTGAATTT GCAC