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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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9-1
(LD)
,
DE(argF-lac)169
,
OCR36,37-6 (LD 9C::Ap)
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
lacZ
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Related strains
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Strains Search
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Search strains by gene name:
lacZ
Search strains by all related name:
lacZ b0344 ECK0341 JW0335
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General information (Go to Linear View: )
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Gene Name
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lacZ
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Alternative name
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b0344,ECK0341,JW0335
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Location, Length
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362,455 - 365,529
(
-
)
;
7.81
min
;
3075
(bp) ,
1024
(aa)
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Product
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beta-D-galactosidase
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Operon Name
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lacZYA
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process
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Function
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enzyme; Degradation of small molecules: Carbon compounds
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Gene Ontology
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GO:0003824
;
catalytic activity ( lacZ )
GO:0004553
;
hydrolase activity, hydrolyzing O-glycosyl compounds ( lacZ )
GO:0004565
;
beta-galactosidase activity ( lacZ )
GO:0005515
;
protein binding ( lacZ )
GO:0005975
;
carbohydrate metabolic process ( lacZ )
GO:0008152
;
metabolic process ( lacZ )
GO:0009341
;
beta-galactosidase complex ( lacZ )
GO:0016787
;
hydrolase activity ( lacZ )
GO:0016798
;
hydrolase activity, acting on glycosyl bonds ( lacZ )
GO:0030246
;
carbohydrate binding ( lacZ )
GO:0043169
;
cation binding ( lacZ )
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PID
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1786539
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EC number
(KEGG Pathway)
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3.2.1.23
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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lacZ
(
EG10527
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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BGAL_ECOLI
(
P00722
)
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- Protein name
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Beta-galactosidase
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- Synonyms
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EC 3.2.1.23, Lactase
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- Gene name
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Name=lacZ; OrderedLocusNames=b0344;
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- Comments
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CATALYTIC ACTIVITY:Hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
SUBUNIT:Homotetramer.
SIMILARITY:Belongs to family 2 of glycosyl hydrolases.
DATABASE:NAME=Worthington enzyme manual; WWW="http://www.worthington-biochem.com/BG/".
DATABASE:NAME=ProZyme technical fact sheet; WWW="http://www.prozyme.com/technical/bgaldata.html".
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Entry name(Acc.no)
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Q8FKG6
(
Q8FKG6
)
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- Protein name
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Beta-galactosidase
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- Synonyms
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EC 3.2.1.23
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- Gene name
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Name=lacZ; OrderedLocusNames=c0459;
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- Comments
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COFACTOR:Zinc (By similarity).
SIMILARITY:Belongs to the zinc-containing alcohol dehydrogenase family.
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Entry name(Acc.no)
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Q8X685
(
Q8X685
)
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- Protein name
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Beta-D-galactosidase.
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- Synonyms
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- Gene name
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Name=lacZ; OrderedLocusNames=z0440;
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- Comments
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SIMILARITY:Contains 1 HTH lacI-type DNA-binding domain.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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lacZ
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain I, Beta-Galactosidase (Chains I-P)
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Beta-galactosidase
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PSI-BLAST
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Beta-galactosidase
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nr
(database updated :
2007.02.20
)
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BLAST
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beta-D-galactosidase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
RGD
ATP_GTP_A
GLYCOSYL_HYDROL_F2_2
GLYCOSYL_HYDROL_F2_1
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MMBR References
|
Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC.
(
6307829
)
|
Genetics 1963;48:157-169
The genetic basis of hypersynthesis of beta-galactoside.
Horiuchi, T., S. Horiuchi, and A. Novick.
|
J. Microbiol. Serol. 1970;16:83-89
Use of autocytotoxic beta-D-galactosides for selective growth of Salmonella typhimurium in the presence of coliforms.
Johnston, M. A., and H. Pivnick.
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EMBO J. 1983;2(4):593-7.
Sequence of the lacZ gene of Escherichia coli.
Kalnins A, Otto K, Ruther U, Muller-Hill B.
(
6313347
)
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Mol Gen Genet. 1969;105(1):74-83.
Mutations conferring quantitative and qualitative increases in beta-galactosidase activity in Escherichia coli.
Langridge J.
(
4899399
)
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J Bacteriol. 1973;114(2):882-4.
Selective autocytotoxicity in a model system of Escherichia coli K-12 recombinants.
Martinez D, Whitehouse F Jr.
(
4574705
)
|
J Bacteriol. 1992;174:6256-6263
DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli.
Shi, W., Y. Zhou, J. Wild, J. Adler, and C. A. Gross.
|
J Bacteriol. 1994;176:1683-1688
An efficient Shine-Dalgarno sequence but not translation is necessary for lacZ mRNA stability in Escherichia coli.
Wagner, L. A., R. F. Gesteland, T. J. Dayhuff, and R. B. Weiss.
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| Sequences |
Amino acid
FASTA format
|
0001 MTMITDSLAV VLQRRDWENP GVTQLNRLAA HPPFASWRNS EEARTDRPSQ QLRSLNGEWR FAWFPAPEAV
0071 PESWLECDLP EADTVVVPSN WQMHGYDAPI YTNVTYPITV NPPFVPTENP TGCYSLTFNV DESWLQEGQT
0141 RIIFDGVNSA FHLWCNGRWV GYGQDSRLPS EFDLSAFLRA GENRLAVMVL RWSDGSYLED QDMWRMSGIF
0211 RDVSLLHKPT TQISDFHVAT RFNDDFSRAV LEAEVQMCGE LRDYLRVTVS LWQGETQVAS GTAPFGGEII
0281 DERGGYADRV TLRLNVENPK LWSAEIPNLY RAVVELHTAD GTLIEAEACD VGFREVRIEN GLLLLNGKPL
0351 LIRGVNRHEH HPLHGQVMDE QTMVQDILLM KQNNFNAVRC SHYPNHPLWY TLCDRYGLYV VDEANIETHG
0421 MVPMNRLTDD PRWLPAMSER VTRMVQRDRN HPSVIIWSLG NESGHGANHD ALYRWIKSVD PSRPVQYEGG
0491 GADTTATDII CPMYARVDED QPFPAVPKWS IKKWLSLPGE TRPLILCEYA HAMGNSLGGF AKYWQAFRQY
0561 PRLQGGFVWD WVDQSLIKYD ENGNPWSAYG GDFGDTPNDR QFCMNGLVFA DRTPHPALTE AKHQQQFFQF
0631 RLSGQTIEVT SEYLFRHSDN ELLHWMVALD GKPLASGEVP LDVAPQGKQL IELPELPQPE SAGQLWLTVR
0701 VVQPNATAWS EAGHISAWQQ WRLAENLSVT LPAASHAIPH LTTSEMDFCI ELGNKRWQFN RQSGFLSQMW
0771 IGDKKQLLTP LRDQFTRAPL DNDIGVSEAT RIDPNAWVER WKAAGHYQAE AALLQCTADT LADAVLITTA
0841 HAWQHQGKTL FISRKTYRID GSGQMAITVD VEVASDTPHP ARIGLNCQLA QVAERVNWLG LGPQENYPDR
0911 LTAACFDRWD LPLSDMYTPY VFPSENGLRC GTRELNYGPH QWRGDFQFNI SRYSQQQLME TSHRHLLHAE
0981 EGTWLNIDGF HMGIGGDDSW SPSVSAEFQL SAGRYHYQLV WCQK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tagctcactc attaggcacc ccaggcttta
-070 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
0001 atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
0071 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
0141 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
0211 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
0281 acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac
0351 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg
0421 cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc
0491 aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt
0561 gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc
0631 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg
0701 atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt
0771 aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc
0841 gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg
0911 ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga
0981 agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg
1051 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
1121 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc
1191 gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc
1261 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa
1331 tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc
1401 taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc
1471 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
1541 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg
1611 cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat
1681 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca
1751 acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct
1821 ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc
1891 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc
1961 actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg
2031 taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc
2101 gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg
2171 cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga
2241 tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
2311 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca
2381 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta
2451 ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct
2521 cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc
2591 aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg
2661 ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
2731 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
2801 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca
2871 gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa
2941 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag
3011 tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
3081 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa aaacacaaac
3151 ttttggatgt tcggtttatt ctttt
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