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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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9-1
(LD)
,
DE(gpt-lac)5
,
DE(argF-lac)169
,
OCR36,37-6 (LD 9C::Ap)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
lacY
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Related strains
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Strains Search
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Search strains by gene name:
lacY
Search strains by all related name:
lacY b0343 ECK0340 JW0334
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General information (Go to Linear View: )
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Gene Name
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lacY
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Alternative name
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b0343,ECK0340,JW0334
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Location, Length
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361,150 - 362,403
(
-
)
;
7.78
min
;
1254
(bp) ,
417
(aa)
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Product
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lactose permease
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Operon Name
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lacZYA
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Note
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galactoside permease (lactose permease, M protein) (MFS family); GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0016052 - carbohydrate catabolic process
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Function
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transport; Transport of small molecules: Carbohydrates, organic acids, alcohols,1.1.1 metabolism; carbon utilization; carbon compounds,4.2.A.1 transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Major Facili
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Gene Ontology
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GO:0005215
;
transporter activity ( lacY )
GO:0005351
;
sugar:hydrogen ion symporter activity ( lacY )
GO:0005403
;
hydrogen:sugar symporter activity ( lacY )
GO:0005886
;
plasma membrane ( lacY )
GO:0006810
;
transport ( lacY )
GO:0008643
;
carbohydrate transport ( lacY )
GO:0015293
;
symporter activity ( lacY )
GO:0016020
;
membrane ( lacY )
GO:0016021
;
integral to membrane ( lacY )
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PID
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1786538
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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LACY_ECOLI
(
P02920
)
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- Protein name
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Lactose permease
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- Synonyms
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Lactose-proton symport
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- Gene name
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Name=lacY; OrderedLocusNames=b0343;
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- Comments
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FUNCTION:Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
SUBUNIT:Monomer.
SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane.
MASS SPECTROMETRY:MW=47357; METHOD=Electrospray; RANGE=1-417; NOTE=Ref.11.
SIMILARITY:Belongs to the lacY/rafB family of permeases.
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Entry name(Acc.no)
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Q8FKG7
(
Q8FKG7
)
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- Protein name
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Lactose permease.
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- Synonyms
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- Gene name
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Name=lacY; OrderedLocusNames=c0458;
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- Comments
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COFACTOR:Zinc (By similarity).
SIMILARITY:Belongs to the zinc-containing alcohol dehydrogenase family.
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Entry name(Acc.no)
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Q8X4M4
(
Q8X4M4
)
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- Protein name
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Galactoside permease
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- Synonyms
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M protein
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- Gene name
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Name=lacY; OrderedLocusNames=z0439;
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- Comments
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SIMILARITY:Belongs to the ABC transporter family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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lacY
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Crystal Structure Of Lactose Permease
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Lactose permease
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PSI-BLAST
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High-affinity glucose transporter SNF3
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nr
(database updated :
2007.02.20
)
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BLAST
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Lactose permease [Escherichia coli CFT073]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
LACY_1
LACY_2
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MMBR References
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Nature. 1980;283(5747):537-40.
In vitro and in vivo products of E. coli lactose permease gene are identical.
Ehring R, Beyreuther K, Wright JK, Overath P.
(
6986561
)
|
Biochemistry. 1996;35(39):12909-14.
Cysteine-scanning mutagenesis of transmembrane domain XII and the flanking periplasmic loop in the lactose permease of EScherichia coli.
He MM, Sun J, Kaback HR.
(
8841135
)
|
Genetics 1963;48:157-169
The genetic basis of hypersynthesis of beta-galactoside.
Horiuchi, T., S. Horiuchi, and A. Novick.
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J. Microbiol. Serol. 1970;16:83-89
Use of autocytotoxic beta-D-galactosides for selective growth of Salmonella typhimurium in the presence of coliforms.
Johnston, M. A., and H. Pivnick.
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Proc Natl Acad Sci U S A. 1968;59(4):1259-64.
Mutants that mke more lac repressor.
Muller-Hill B, Crapo L, Gilbert W.
(
4870861
)
|
J Bacteriol. 1993;175:6269-6275
Lactose permease mutants which transport malto-oligosaccharides.
Olsen, S. G., K. M. Greene, and R. J. Brooker.
|
Proc Natl Acad Sci U S A. 1992;89:1524-1528
Functional complementation of internal deletion mutants in the lactose permease of Escherichia coli.
Bibi, E., and H. R. Kaback.
|
Prog Nucleic Acid Res Mol Biol. 1996;55:1-86.
Experimental analysis of global gene regulation in Escherichia coli.
Blumenthal RM, Borst DW, Matthews RG.
(
8787606
)
|
J Mol Biol. 1971;59(2):273-305.
The nature of lactose operator constitive mutations.
Smith TF, Sadler JR.
(
4935786
)
|
J Bacteriol. 1980;143(3):1374-83.
Pleiotropic mutations rendering Escherichia coli K-12 resistant to bacteriophage TP1.
Wandersman C, Moreno F, Schwartz M.
(
6997269
)
|
Nature. 1980;283(5747):541-5.
Sequence of the lactose permease gene.
Buchel DE, Gronenborn B, Muller-Hill B.
(
6444453
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MYYLKNTNFW MFGLFFFFYF FIMGAYFPFF PIWLHDINHI SKSDTGIIFA AISLFSLLFQ PLFGLLSDKL
0071 GLRKYLLWII TGMLVMFAPF FIFIFGPLLQ YNILVGSIVG GIYLGFCFNA GAPAVEAFIE KVSRRSNFEF
0141 GRARMFGCVG WALCASIVGI MFTINNQFVF WLGSGCALIL AVLLFFAKTD APSSATVANA VGANHSAFSL
0211 KLALELFRQP KLWFLSLYVI GVSCTYDVFD QQFANFFTSF FATGEQGTRV FGYVTTMGEL LNASIMFFAP
0281 LIINRIGGKN ALLLAGTIMS VRIIGSSFAT SALEVVILKT LHMFEVPFLL VGCFKYITSQ FEVRFSATIY
0351 LVCFCFFKQL AMIFMSVLAG NMYESIGFQG AYLVLGLVAL GFTLISVFTL SGPGPLSLLR RQVNEVA
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gctgagcgcc ggtcgctacc attaccagtt
-070 ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag gaaatccatt
0001 atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt tttatcatgg
0071 gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc agcaaaagtg atacgggtat
0141 tatttttgcc gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc
0211 gggctgcgca aatacctgct gtggattatt accggcatgt tagtgatgtt tgcgccgttc tttattttta
0281 tcttcgggcc actgttacaa tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg
0351 ttttaacgcc ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt
0421 ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc atgttcacca
0491 tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc gccgttttac tctttttcgc
0561 caaaacggat gcgccctctt ctgccacggt tgccaatgcg gtaggtgcca accattcggc atttagcctt
0631 aagctggcac tggaactgtt cagacagcca aaactgtggt ttttgtcact gtatgttatt ggcgtttcct
0701 gcacctacga tgtttttgac caacagtttg ctaatttctt tacttcgttc tttgctaccg gtgaacaggg
0771 tacgcgggta tttggctacg taacgacaat gggcgaatta cttaacgcct cgattatgtt ctttgcgcca
0841 ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc tggctggcac tattatgtct gtacgtatta
0911 ttggctcatc gttcgccacc tcagcgctgg aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc
0981 gttcctgctg gtgggctgct ttaaatatat taccagccag tttgaagtgc gtttttcagc gacgatttat
1051 ctggtctgtt tctgcttctt taagcaactg gcgatgattt ttatgtctgt actggcgggc aatatgtatg
1121 aaagcatcgg tttccagggc gcttatctgg tgctgggtct ggtggcgctg ggcttcacct taatttccgt
1191 gttcacgctt agcggccccg gcccgctttc cctgctgcgt cgtcaggtga atgaagtcgc ttaagcaatc
1261 aatgtcggat gcggcgcgag cgccttatcc gaccaacata tcataacgga gtgatcgcat tgaacatgcc
1331 aatgaccgaa agaataagag cagg
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