MG1655
W3110
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Gene Report : lacA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 9-1 (LD)  ,  DE(gpt-lac)5  ,  DE(argF-lac)169  ,  OCR36,37-6 (LD 9C::Ap) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  lacA
Related strains
     Strains Search Search strains by gene name:  lacA   Search strains by all related name:  lacA b0342 ECK0339 f203 JW0333

General information  (Go to Linear View:)
 Gene Name lacA  
 Alternative name b0342,ECK0339,f203,JW0333  
 Location, Length 360,473 - 361,084 (  -  ) ;   7.77 min ; 612 (bp) ,   203 (aa) Go to Linear View
 Product thiogalactoside acetyltransferase  
 Operon Name lacZYA  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function enzyme; Degradation of small molecules: Carbon compounds  
 Gene Ontology GO:0005737; cytoplasm ( lacA )
GO:0005989; lactose biosynthetic process ( lacA )
GO:0008415; acyltransferase activity ( lacA )
GO:0008870; galactoside O-acetyltransferase activity ( lacA )
GO:0016740; transferase activity ( lacA )
 PID 1786537  
 EC number
  (KEGG Pathway)
 
2.3.1.18  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene lacA ( EG10524 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) THGA_ECOLI ( P07464 ; P77862 )
    -  Protein name Galactoside O-acetyltransferase  
    -  Synonyms EC 2.3.1.18, GAT, Thiogalactoside acetyltransferase  
    -  Gene name Name=lacA; OrderedLocusNames=b0342;  
    -  Comments
  • FUNCTION:May assist cellular detoxification by acetylating nonmetabolizable pyranosides, thereby preventing their reentry into the cell.
  • CATALYTIC ACTIVITY:Acetyl-CoA + a beta-D-galactoside = CoA + a 6-acetyl-beta-D-galactoside.
  • PATHWAY:Lactose biosynthesis.
  • SUBUNIT:Homodimer.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • PTM:The amino terminus of this protein is heterogenous because the initiator methionine is only partially cleaved.
  • SIMILARITY:Belongs to the transferase hexapeptide repeat family.  
  •   botton Entry name(Acc.no) Q8FKG8 ( Q8FKG8 )
        -  Protein name Galactoside O-acetyltransferase  
        -  Synonyms EC 2.3.1.18  
        -  Gene name Name=lacA; OrderedLocusNames=c0457;  
        -  Comments
  • COFACTOR:Zinc (By similarity).
  • SIMILARITY:Belongs to the zinc-containing alcohol dehydrogenase family.  
  •   botton Entry name(Acc.no) Q8X686 ( Q8X686 )
        -  Protein name Thiogalactoside acetyltransferase.  
        -  Synonyms  
        -  Gene name Name=lacA; OrderedLocusNames=z0438;  
        -  Comments
  • SIMILARITY:Contains 1 HTH lacI-type DNA-binding domain.  

  •  Linear View (Whole Mode)
    View Location
       350.0  –  375.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP lacA (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Galactoside Acetyltransferase In Complex With Coenzyme A  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Galactoside O-acetyltransferase  
             PSI-BLAST UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
        nr     (database updated : 2007.02.20 )
             BLAST galactoside O-acetyltransferase [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    HEXAPEP_TRANSFERASES     

    Other Cross-Reference
        COG COG0110R 
        EcoCyc lacA 

    MMBR References
    Proc Natl Acad Sci U S A. 1985;82:6414-6418
    DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region.
    Hediger, M. A., D. F. Johnson, D. P. Nierlich, and I. Zabin.
    Genetics 1963;48:157-169
    The genetic basis of hypersynthesis of beta-galactoside.
    Horiuchi, T., S. Horiuchi, and A. Novick.

    Sequences
    Amino acid
    FASTA format
    0001 MNMPMTERIR AGKLFTDMCE GLPEKRLRGK TLMYEFNHSH PSEVEKRESL IKEMFATVGE NAWVEPPVYF 
    0071 SYGSNIHIGR NFYANFNLTI VDDYTVTIGD NVLIAPNVTL SVTGHPVHHE LRKNGEMYSF PITIGNNVWI 
    0141 GSHVVINPGV TIGDNSVIGA GSIVTKDIPP NVVAAGVPCR VIREINDRDK HYYFKDYKVE SSV
    
    
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp
    -100                                             ccctgctgcg tcgtcaggtg aatgaagtcg 
    -070 cttaagcaat caatgtcgga tgcggcgcga gcgccttatc cgaccaacat atcataacgg agtgatcgca 
    0001 ttgaacatgc caatgaccga aagaataaga gcaggcaagc tatttaccga tatgtgcgaa ggcttaccgg 
    0071 aaaaaagact tcgtgggaaa acgttaatgt atgagtttaa tcactcgcat ccatcagaag ttgaaaaaag 
    0141 agaaagcctg attaaagaaa tgtttgccac ggtaggggaa aacgcctggg tagaaccgcc tgtctatttc 
    0211 tcttacggtt ccaacatcca tataggccgc aatttttatg caaatttcaa tttaaccatt gtcgatgact 
    0281 acacggtaac aatcggtgat aacgtactga ttgcacccaa cgttactctt tccgttacgg gacaccctgt 
    0351 acaccatgaa ttgagaaaaa acggcgagat gtactctttt ccgataacga ttggcaataa cgtctggatc 
    0421 ggaagtcatg tggttattaa tccaggcgtc accatcgggg ataattctgt tattggcgcg ggtagtatcg 
    0491 tcacaaaaga cattccacca aacgtcgtgg cggctggcgt tccttgtcgg gttattcgcg aaataaacga 
    0561 ccgggataag cactattatt tcaaagatta taaagttgaa tcgtcagttt aaattataaa aattgcctga 
    0631 tacgctgcgc ttatcaggcc tacaagttca gcgatctaca ttagccgcat ccggcatgaa caaagcgcag 
    0701 gaacaagcgt cg