MG1655
W3110
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Gene Report : carB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR28 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  carB
Related strains
     Strains Search Search strains by gene name:  carB   Search strains by all related name:  carB arg+ura b0033 cap ECK0034 JW0031 pyrA

General information  (Go to Linear View:)
 Gene Name carB  
 Alternative name arg+ura,b0033,cap,ECK0034,JW0031,pyrA  
 Location, Length 30,817 - 34,038 (  +  ) ;   0.66 min ; 3222 (bp) ,   1073 (aa) Go to Linear View
 Product carbamoyl-phosphate synthase large subunit  
 Operon Name carAB  
 Note GO_process: GO:0006526 - arginine biosynthetic process; GO_process: GO:0006221 - pyrimidine nucleotide biosynthetic process  
 Function enzyme; Pyrimidine ribonucleotide biosynthesis,1.5.1.3 metabolism; building block biosynthesis; amino acids; arginine,1.5.2.2 metabolism; building block biosynthesis; nucleotide; pyrimidine biosynthesis  
 Gene Ontology GO:0000166; nucleotide binding ( carB )
GO:0003824; catalytic activity ( carB )
GO:0004086; carbamoyl-phosphate synthase activity ( carB )
GO:0004088; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity ( carB )
GO:0005515; protein binding ( carB )
GO:0005524; ATP binding ( carB )
GO:0006221; pyrimidine nucleotide biosynthetic process ( carB )
GO:0006526; arginine biosynthetic process ( carB )
GO:0006807; nitrogen compound metabolic process ( carB )
GO:0008152; metabolic process ( carB )
GO:0008652; amino acid biosynthetic process ( carB )
GO:0016874; ligase activity ( carB )
GO:0030145; manganese ion binding ( carB )
GO:0046872; metal ion binding ( carB )
 PID 1786216  
 EC number
  (KEGG Pathway)
 
6.3.5.5  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene carB ( EG10135 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) CARA_ECOLI ( P00907 )
    -  Protein name Carbamoyl-phosphate synthase small chain  
    -  Synonyms EC 6.3.5.5, Carbamoyl- phosphate synthetase glutamine chain  
    -  Gene name Name=carA; Synonyms=pyrA; OrderedLocusNames=b0032, z0037, ECs0035;  
    -  Comments
  • CATALYTIC ACTIVITY:2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
  • PATHWAY:Arginine biosynthesis.
  • PATHWAY:Pyrimidine biosynthesis; first step.
  • SUBUNIT:Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
  • SIMILARITY:Belongs to the carA family.
  • SIMILARITY:Contains 1 type-1 glutamine amidotransferase domain.  
  •   botton Entry name(Acc.no) CARB_ECOLI ( P00968 )
        -  Protein name Carbamoyl-phosphate synthase large chain  
        -  Synonyms EC 6.3.5.5, Carbamoyl- phosphate synthetase ammonia chain  
        -  Gene name Name=carB; Synonyms=pyrA; OrderedLocusNames=b0033;  
        -  Comments
  • CATALYTIC ACTIVITY:2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
  • COFACTOR:Binds 3 manganese ions per subunit.
  • PATHWAY:Arginine biosynthesis.
  • PATHWAY:Pyrimidine biosynthesis; first step.
  • SUBUNIT:Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
  • SIMILARITY:Belongs to the carB family.
  • SIMILARITY:Contains 2 ATP-grasp domains.  
  •   botton Entry name(Acc.no) YAAV_ECOLI ( P46145 )
        -  Protein name Hypothetical protein yaaV.  
        -  Synonyms  
        -  Gene name Name=yaaV; OrderedLocusNames=b0033.1;  
        -  Comments
  • SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane (Potential).
  • SIMILARITY:Belongs to the sugar transporter family.
  • CAUTION:Ref.1 sequence differs from that shown due to frameshifts in positions 142 and 232 that produce two separate ORFs.  
  •   botton Entry name(Acc.no) CARB_ECOL6 ( Q8FLB0 )
        -  Protein name Carbamoyl-phosphate synthase large chain  
        -  Synonyms EC 6.3.5.5, Carbamoyl- phosphate synthetase ammonia chain  
        -  Gene name Name=carB; OrderedLocusNames=c0041;  
        -  Comments
  • CATALYTIC ACTIVITY:2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
  • COFACTOR:Binds 3 manganese ions per subunit (By similarity).
  • PATHWAY:Arginine biosynthesis.
  • PATHWAY:Pyrimidine biosynthesis; first step.
  • SUBUNIT:Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4 (By similarity).
  • SIMILARITY:Belongs to the carB family.
  • SIMILARITY:Contains 2 ATP-grasp domains.  
  •   botton Entry name(Acc.no) CARB_ECO57 ( Q8XA38 )
        -  Protein name Carbamoyl-phosphate synthase large chain  
        -  Synonyms EC 6.3.5.5, Carbamoyl- phosphate synthetase ammonia chain  
        -  Gene name Name=carB; OrderedLocusNames=z0038, ECs0036, SF0030, S0032;  
        -  Comments
  • CATALYTIC ACTIVITY:2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
  • COFACTOR:Binds 3 manganese ions per subunit (By similarity).
  • PATHWAY:Arginine biosynthesis.
  • PATHWAY:Pyrimidine biosynthesis; first step.
  • SUBUNIT:Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4 (By similarity).
  • SIMILARITY:Belongs to the carB family.
  • SIMILARITY:Contains 2 ATP-grasp domains.  

  •  Linear View (Whole Mode)
    View Location
       20.0  –  45.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP carB (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Carbamoyl Phosphate Synthetase From Escherichis Coli With Complexed With The Allosteric Ligand Imp  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Carbamoyl-phosphate synthase large chain  
             PSI-BLAST Carbamoyl-phosphate synthase large chain
        nr     (database updated : 2007.02.20 )
             BLAST carbamoyl-phosphate synthase large subunit [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    PROKAR_LIPOPROTEIN    ATP_GTP_A    CPSASE_1    CPSASE_2     

    Other Cross-Reference
        COG COG0458EF 
        EcoCyc carB 

    MMBR References
    J Bacteriol. 1980;143(2):914-20.
    Patterns of polarity in the Escherichia coli car AB gene cluster.
    Gigot D, Crabeel M, Feller A, Charlier D, Lissens W, Glansdorff N, Pierard A. ( 6162837 )
    Proc Natl Acad Sci U S A. 1983;80:4629-4633
    The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.
    Nyunoya, H., and C. J. Lusty.
    J Bacteriol. 1980;143(2):921-5.
    Use of gene cloning to determine polarity of an operon: genes carAB of Escherichia coli.
    Crabeel M, Charlier D, Weyens G, Feller A, Pierard A, Glansdorff N. ( 6451616 )

    Sequences
    Amino acid
    FASTA format
    0001 MPKRTDIKSI LILGAGPIVI GQACEFDYSG AQACKALREE GYRVILVNSN PATIMTDPEM ADATYIEPIH 
    0071 WEVVRKIIEK ERPDAVLPTM GGQTALNCAL ELERQGVLEE FGVTMIGATA DAIDKAEDRR RFDVAMKKIG 
    0141 LETARSGIAH TMEEALAVAA DVGFPCIIRP SFTMGGSGGG IAYNREEFEE ICARGLDLSP TKELLIDESL 
    0211 IGWKEYEMEV VRDKNDNCII VCSIENFDAM GIHTGDSITV APAQTLTDKE YQIMRNASMA VLREIGVETG 
    0281 GSNVQFAVNP KNGRLIVIEM NPRVSRSSAL ASKATGFPIA KVAAKLAVGY TLDELMNDIT GGRTPASFEP 
    0351 SIDYVVTKIP RFNFEKFAGA NDRLTTQMKS VGEVMAIGRT QQESLQKALR GLEVGATGFD PKVSLDDPEA 
    0421 LTKIRRELKD AGADRIWYIA DAFRAGLSVD GVFNLTNIDR WFLVQIEELV RLEEKVAEVG ITGLNADFLR 
    0491 QLKRKGFADA RLAKLAGVRE AEIRKLRDQY DLHPVYKRVD TCAAEFATDT AYMYSTYEEE CEANPSTDRE 
    0561 KIMVLGGGPN RIGQGIEFDY CCVHASLALR EDGYETIMVN CNPETVSTDY DTSDRLYFEP VTLEDVLEIV 
    0631 RIEKPKGVIV QYGGQTPLKL ARALEAAGVP VIGTSPDAID RAEDRERFQH AVERLKLKQP ANATVTAIEM 
    0701 AVEKAKEIGY PLVVRPSYVL GGRAMEIVYD EADLRRYFQT AVSVSNDAPV LLDHFLDDAV EVDVDAICDG 
    0771 EMVLIGGIME HIEQAGVHSG DSACSLPAYT LSQEIQDVMR QQVQKLAFEL QVRGLMNVQF AVKNNEVYLI 
    0841 EVNPRAARTV PFVSKATGVP LAKVAARVMA GKSLAEQGVT KEVIPPYYSV KEVVLPFNKF PGVDPLLGPE 
    0911 MRSTGEVMGV GRTFAEAFAK AQLGSNSTMK KHGRALLSVR EGDKERVVDL AAKLLKQGFE LDATHGTAIV 
    0981 LGEAGINPRL VNKVHEGRPH IQDRIKNGEY TYIINTTSGR RAIEDSRVIR RSALQYKVHY DTTLNGGFAT 
    1051 AMALNADATE KVISVQEMHA QIK
    
    
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp
    -100                                             CCAGCCCTGG TCCACACGAC GCCGCGCCGT 
    -070 TGTTCGACCA CTTTATCGAG TTAATTGAGC AGTACCGTAA AACCGCTAAG TAATCAGGAG TAAAAGAGCC 
    0001 atgccaaaac gtacagatat aaaaagtatc ctgattctgg gtgcgggccc gattgttatc ggtcaggcgt 
    0071 gtgagtttga ctactctggc gcgcaagcgt gtaaagccct gcgtgaagag ggttaccgcg tcattctggt 
    0141 gaactccaac ccggcgacca tcatgaccga cccggaaatg gctgatgcaa cctacatcga gccgattcac 
    0211 tgggaagttg tacgcaagat tattgaaaaa gagcgcccgg acgcggtgct gccaacgatg ggcggtcaga 
    0281 cggcgctgaa ctgcgcgctg gagctggaac gtcagggcgt gttggaagag ttcggtgtca ccatgattgg 
    0351 tgccactgcc gatgcgattg ataaagcaga agaccgccgt cgtttcgacg tagcgatgaa gaaaattggt 
    0421 ctggaaaccg cgcgttccgg tatcgcacac acgatggaag aagcgctggc ggttgccgct gacgtgggct 
    0491 tcccgtgcat tattcgccca tcctttacca tgggcggtag cggcggcggt atcgcttata accgtgaaga 
    0561 gtttgaagaa atttgcgccc gcggtctgga tctctctccg accaaagagt tgctgattga tgagtcgctg 
    0631 atcggctgga aagagtacga gatggaagtg gtgcgtgata aaaacgacaa ctgcatcatc gtctgctcta 
    0701 tcgaaaactt cgatgcgatg ggcatccaca ccggtgactc catcactgtc gcgccagccc aaacgctgac 
    0771 cgacaaagaa tatcaaatca tgcgtaacgc ctcgatggcg gtgctgcgtg aaatcggcgt tgaaaccggt 
    0841 ggttccaacg ttcagtttgc ggtgaacccg aaaaacggtc gtctgattgt tatcgaaatg aacccacgcg 
    0911 tgtcccgttc ttcggcgctg gcgtcgaaag cgaccggttt cccgattgct aaagtggcgg cgaaactggc 
    0981 ggtgggttac accctcgacg aactgatgaa cgacatcact ggcggacgta ctccggcctc cttcgagccg 
    1051 tccatcgact atgtggttac taaaattcct cgcttcaact tcgaaaaatt cgccggtgct aacgaccgtc 
    1121 tgaccactca gatgaaatcg gttggcgaag tgatggcgat tggtcgcacg cagcaggaat ccctgcaaaa 
    1191 agcgctgcgc ggcctggaag tcggtgcgac tggattcgac ccgaaagtga gcctggatga cccggaagcg 
    1261 ttaaccaaaa tccgtcgcga actgaaagac gcaggcgcag atcgtatctg gtacatcgcc gatgcgttcc 
    1331 gtgcgggcct gtctgtggac ggcgtcttca acctgaccaa cattgaccgc tggttcctgg tacagattga 
    1401 agagctggtg cgtctggaag agaaagtggc ggaagtgggc atcactggcc tgaacgctga cttcctgcgc 
    1471 cagctgaaac gcaaaggctt tgccgatgcg cgcttggcaa aactggcggg cgtacgcgaa gcggaaatcc 
    1541 gtaagctgcg tgaccagtat gacctgcacc cggtttataa gcgcgtggat acctgtgcgg cagagttcgc 
    1611 caccgacacc gcttacatgt actccactta tgaagaagag tgcgaagcga atccgtctac cgaccgtgaa 
    1681 aaaatcatgg tgcttggcgg cggcccgaac cgtatcggtc agggtatcga attcgactac tgttgcgtac 
    1751 acgcctcgct ggcgctgcgc gaagacggtt acgaaaccat tatggttaac tgtaacccgg aaaccgtctc 
    1821 caccgactac gacacttccg accgcctcta cttcgagccg gtaactctgg aagatgtgct ggaaatcgtg 
    1891 cgtatcgaga agccgaaagg cgttatcgtc cagtacggcg gtcagacccc gctgaaactg gcgcgcgcgc 
    1961 tggaagctgc tggcgtaccg gttatcggca ccagcccgga tgctatcgac cgtgcagaag accgtgaacg 
    2031 cttccagcat gcggttgagc gtctgaaact gaaacaaccg gcgaacgcca ccgttaccgc tattgaaatg 
    2101 gcggtagaga aggcgaaaga gattggctac ccgctggtgg tacgtccgtc ttacgttctc ggcggtcggg 
    2171 cgatggaaat cgtctatgac gaagctgacc tgcgtcgcta cttccagacg gcggtcagcg tgtctaacga 
    2241 tgcgccagtg ttgctggacc acttcctcga tgacgcggta gaagttgacg tggatgccat ctgcgacggc 
    2311 gaaatggtgc tgattggcgg catcatggag catattgagc aggcgggcgt gcactccggt gactccgcat 
    2381 gttctctgcc agcctacacc ttaagtcagg aaattcagga tgtgatgcgc cagcaggtgc agaaactggc 
    2451 cttcgaattg caggtgcgcg gcctgatgaa cgtgcagttt gcggtgaaaa acaacgaagt ctacctgatt 
    2521 gaagttaacc cgcgtgcggc gcgtaccgtt ccgttcgtct ccaaagccac cggcgtaccg ctggcaaaag 
    2591 tggcggcgcg cgtgatggct ggcaaatcgc tggctgagca gggcgtaacc aaagaagtta tcccgccgta 
    2661 ctactcggtg aaagaagtgg tgctgccgtt caataaattc ccgggcgttg acccgctgtt agggccagaa 
    2731 atgcgctcta ccggggaagt catgggcgtg ggccgcacct tcgctgaagc gtttgccaaa gcgcagctgg 
    2801 gcagcaactc caccatgaag aaacacggtc gtgcgctgct ttccgtgcgc gaaggcgata aagaacgcgt 
    2871 ggtggacctg gcggcaaaac tgctgaaaca gggcttcgag ctggatgcga cccacggcac ggcgattgtg 
    2941 ctgggcgaag caggtatcaa cccgcgtctg gtaaacaagg tgcatgaagg ccgtccgcac attcaggacc 
    3011 gtatcaagaa tggcgaatat acctacatca tcaacaccac ctcaggccgt cgtgcgattg aagactcccg 
    3081 cgtgattcgt cgcagtgcgc tgcaatataa agtgcattac gacaccaccc tgaacggcgg ctttgccacc 
    3151 gcgatggcgc tgaatgccga tgcgactgaa aaagtaattt cggtgcagga aatgcacgca cagatcaaat 
    3221 aaTAGCGTGT CATGGCAGAT ATTTTTCATC CGCTAATTTG ATCGAATAAC TAATACGGTT CTCTGATGAG 
    3291 GACCGTTTTT TTTTGCCCAT TAAGTAAATC TT