|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Mol Microbiol. 1997;25(4):661-70.
Overinitiation of chromosome replication in the Escherichia coli dnaAcos mutant depends on activation of oriC function by the dam gene product.
Katayama T, Akimitsu N, Mizushima T, Miki T, Sekimizu K.
(
9379896
)
|
|
|
Deletion
|
OCL19,20-1
(D)
,
OCL19,20-2
(D)
,
OCL19,20-3
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
aroB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
aroB
Search strains by all related name:
aroB b3389 ECK3376 f362 JW3352
|
General information (Go to Linear View: )
|
|
Gene Name
|
aroB
|
|
Alternative name
|
b3389,ECK3376,f362,JW3352
|
|
Location, Length
|
3,515,420 - 3,516,508
(
-
)
;
75.77
min
;
1089
(bp) ,
362
(aa)
|
|
Product
|
3-dehydroquinate synthase
|
|
Operon Name
|
aroKB-damX-dam-rpe-gph-trpS
|
|
Note
|
GO_process: GO:0009423 - chorismate biosynthetic process
|
|
Function
|
enzyme; Amino acid biosynthesis: Chorismate
|
|
Gene Ontology
|
GO:0003856
;
3-dehydroquinate synthase activity ( aroB )
GO:0005737
;
cytoplasm ( aroB )
GO:0008652
;
amino acid biosynthetic process ( aroB )
GO:0009073
;
aromatic amino acid family biosynthetic process ( aroB )
GO:0016829
;
lyase activity ( aroB )
GO:0050897
;
cobalt ion binding ( aroB )
|
|
PID
|
1789791
|
EC number
(KEGG Pathway)
|
4.2.3.4
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
AROB_ECOLI
(
P07639
)
|
|
- Protein name
|
3-dehydroquinate synthase
|
|
- Synonyms
|
EC 4.2.3.4
|
|
- Gene name
|
Name=aroB; OrderedLocusNames=b3389;
|
|
- Comments
|
CATALYTIC ACTIVITY:3-deoxy-arabino-heptulosonate 7-phosphate = 3-dehydroquinate + phosphate.
COFACTOR:NAD and a divalent metal cation such as cobalt.
PATHWAY:Aromatic amino acids biosynthesis; shikimate pathway; second step.
SUBUNIT:Monomer.
SUBCELLULAR LOCATION:Cytoplasmic.
SIMILARITY:Belongs to the dehydroquinate synthase family.
|
|
Entry name(Acc.no)
|
AROB_ECOL6
(
Q8FCV7
)
|
|
- Protein name
|
3-dehydroquinate synthase
|
|
- Synonyms
|
EC 4.2.3.4
|
|
- Gene name
|
Name=aroB; OrderedLocusNames=c4159;
|
|
- Comments
|
CATALYTIC ACTIVITY:3-deoxy-arabino-heptulosonate 7-phosphate = 3-dehydroquinate + phosphate.
COFACTOR:NAD and a divalent metal cation such as cobalt (By similarity).
PATHWAY:Aromatic amino acids biosynthesis; shikimate pathway; second step.
SUBUNIT:Monomer (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (Probable).
SIMILARITY:Belongs to the dehydroquinate synthase family.
|
|
Entry name(Acc.no)
|
AROB_ECO57
(
Q8X824
)
|
|
- Protein name
|
3-dehydroquinate synthase
|
|
- Synonyms
|
EC 4.2.3.4
|
|
- Gene name
|
Name=aroB; OrderedLocusNames=z4742, ECs4231;
|
|
- Comments
|
CATALYTIC ACTIVITY:3-deoxy-arabino-heptulosonate 7-phosphate = 3-dehydroquinate + phosphate.
COFACTOR:NAD and a divalent metal cation such as cobalt (By similarity).
PATHWAY:Aromatic amino acids biosynthesis; shikimate pathway; second step.
SUBUNIT:Monomer (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (Probable).
SIMILARITY:Belongs to the dehydroquinate synthase family.
|
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
aroB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Staphlyococcus Aureus 3-Dehydroquinate Synthase (Dhqs) In Complex With Zn2+, Nad+ And Carbaphosphonate
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
3-dehydroquinate synthase
|
|
PSI-BLAST
|
3-dehydroquinate synthase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
3-dehydroquinate synthase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
FEBS Lett. 1986;200(1):11-7.
The complete amino acid sequence of 3-dehydroquinate synthase of Escherichia coli K12.
Millar G, Coggins JR.
(
3009224
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MERIVVTLGE RSYPITIASG LFNEPASFLP LKSGEQVMLV TNETLAPLYL DKVRGVLEQA GVNVDSVILP
0071 DGEQYKSLAV LDTVFTALLQ KPHGRDTTLV ALGGGVVGDL TGFAAASYQR GVRFIQVPTT LLSQVDSSVG
0141 GKTAVNHPLG KNMIGAFYQP ASVVVDLDCL KTLPPRELAS GLAEVIKYGI ILDGAFFNWL EENLDALLRL
0211 DGPAMAYCIR RCCELKAEVV AADERETGLR ALLNLGHTFG HAIEAEMGYG NWLHGEAVAA GMVMAARTSE
0281 RLGQFSSAET QRIITLLKRA GLPVNGPREM SAQAYLPHML RDKKVLAGEM RLILPLAIGK SEVRSGVSHE
0351 LVLNAIADCQ SA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 aagtggttgc aaaccagatt attcacatgc
-070 tggaaagcaa ctaattctgg ctttatatac actcgtctgc gggtacagta attaaggtgg atgtcgcgtt
0001 atggagagga ttgtcgttac tctcggggaa cgtagttacc caattaccat cgcatctggt ttgtttaatg
0071 aaccagcttc attcttaccg ctgaaatcgg gcgagcaggt catgttggtc accaacgaaa ccctggctcc
0141 tctgtatctc gataaggtcc gcggcgtact tgaacaggcg ggtgttaacg tcgatagcgt tatcctccct
0211 gacggcgagc agtataaaag cctggctgta ctcgataccg tctttacggc gttgttacaa aaaccgcatg
0281 gtcgcgatac tacgctggtg gcgcttggcg gcggcgtagt gggcgatctg accggcttcg cggcggcgag
0351 ttatcagcgc ggtgtccgtt tcattcaagt cccgacgacg ttactgtcgc aggtcgattc ctccgttggc
0421 ggcaaaactg cggtcaacca tcccctcggt aaaaacatga ttggcgcgtt ctaccaacct gcttcagtgg
0491 tggtggatct cgactgtctg aaaacgcttc ccccgcgtga gttagcgtcg gggctggcag aagtcatcaa
0561 atacggcatt attcttgacg gtgcgttttt taactggctg gaagagaatc tggatgcgtt gttgcgtctg
0631 gacggtccgg caatggcgta ctgtattcgc cgttgttgtg aactgaaggc agaagttgtc gccgccgacg
0701 agcgcgaaac cgggttacgt gctttactga atctgggaca cacctttggt catgccattg aagctgaaat
0771 ggggtatggc aattggttac atggtgaagc ggtcgctgcg ggtatggtga tggcggcgcg gacgtcggaa
0841 cgtctcgggc agtttagttc tgccgaaacg cagcgtatta taaccctgct caagcgggct gggttaccgg
0911 tcaatgggcc gcgcgaaatg tccgcgcagg cgtatttacc gcatatgctg cgtgacaaga aagtccttgc
0981 gggagagatg cgcttaattc ttccgttggc aattggtaag agtgaagttc gcagcggcgt ttcgcacgag
1051 cttgttctta acgccattgc cgattgtcaa tcagcgtaac aacaagaaag gtcaggccgc ttatcaagcg
1121 gtctattagc ttcaggttaa ttgcaacgtg gtaagcatta accttttagt ggggtgttaa atggatgaa
|
