MG1655
W3110
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Gene Report : carA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR28 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  carA
Related strains
     Strains Search Search strains by gene name:  carA   Search strains by all related name:  carA arg arg+ura b0032 cap ECK0033 JW0030 pyrA

General information  (Go to Linear View:)
 Gene Name carA  
 Alternative name arg,arg+ura,b0032,cap,ECK0033,JW0030,pyrA  
 Location, Length 29,651 - 30,799 (  +  ) ;   0.64 min ; 1149 (bp) ,   382 (aa) Go to Linear View
 Product carbamoyl phosphate synthetase small subunit, glutamine amidotransferase  
 Operon Name carAB  
 Note GO_process: GO:0006526 - arginine biosynthetic process; GO_process: GO:0006221 - pyrimidine nucleotide biosynthetic process  
 Function enzyme; Pyrimidine ribonucleotide biosynthesis,1.5.1.3 metabolism; building block biosynthesis; amino acids; arginine,1.5.2.2 metabolism; building block biosynthesis; nucleotide; pyrimidine biosynthesis  
 Gene Ontology GO:0003824; catalytic activity ( carA )
GO:0004086; carbamoyl-phosphate synthase activity ( carA )
GO:0004088; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity ( carA )
GO:0005515; protein binding ( carA )
GO:0005524; ATP binding ( carA )
GO:0006221; pyrimidine nucleotide biosynthetic process ( carA )
GO:0006526; arginine biosynthetic process ( carA )
GO:0006541; glutamine metabolic process ( carA )
GO:0006807; nitrogen compound metabolic process ( carA )
GO:0008652; amino acid biosynthetic process ( carA )
GO:0009058; biosynthetic process ( carA )
GO:0016874; ligase activity ( carA )
 PID 1786215  
 EC number
  (KEGG Pathway)
 
6.3.5.5  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene carA ( EG10134 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) CARA_ECOLI ( P00907 )
    -  Protein name Carbamoyl-phosphate synthase small chain  
    -  Synonyms EC 6.3.5.5, Carbamoyl- phosphate synthetase glutamine chain  
    -  Gene name Name=carA; Synonyms=pyrA; OrderedLocusNames=b0032, z0037, ECs0035;  
    -  Comments
  • CATALYTIC ACTIVITY:2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
  • PATHWAY:Arginine biosynthesis.
  • PATHWAY:Pyrimidine biosynthesis; first step.
  • SUBUNIT:Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
  • SIMILARITY:Belongs to the carA family.
  • SIMILARITY:Contains 1 type-1 glutamine amidotransferase domain.  
  •   botton Entry name(Acc.no) CARB_ECOLI ( P00968 )
        -  Protein name Carbamoyl-phosphate synthase large chain  
        -  Synonyms EC 6.3.5.5, Carbamoyl- phosphate synthetase ammonia chain  
        -  Gene name Name=carB; Synonyms=pyrA; OrderedLocusNames=b0033;  
        -  Comments
  • CATALYTIC ACTIVITY:2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
  • COFACTOR:Binds 3 manganese ions per subunit.
  • PATHWAY:Arginine biosynthesis.
  • PATHWAY:Pyrimidine biosynthesis; first step.
  • SUBUNIT:Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
  • SIMILARITY:Belongs to the carB family.
  • SIMILARITY:Contains 2 ATP-grasp domains.  
  •   botton Entry name(Acc.no) CARA_ECOL6 ( Q8FLB1 )
        -  Protein name Carbamoyl-phosphate synthase small chain  
        -  Synonyms EC 6.3.5.5, Carbamoyl- phosphate synthetase glutamine chain  
        -  Gene name Name=carA; OrderedLocusNames=c0040;  
        -  Comments
  • CATALYTIC ACTIVITY:2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
  • PATHWAY:Arginine biosynthesis.
  • PATHWAY:Pyrimidine biosynthesis; first step.
  • SUBUNIT:Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate (By similarity).
  • SIMILARITY:Belongs to the carA family.
  • SIMILARITY:Contains 1 type-1 glutamine amidotransferase domain.  

  •  Linear View (Whole Mode)
    View Location
       20.0  –  45.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP carA (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain C, Carbamoyl Phosphate Synthetase From Escherichia Coli  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Carbamoyl-phosphate synthase small chain (Carbamoyl-phosphate synthetase glutamine chain)  
             PSI-BLAST Carbamoyl-phosphate synthase small chain
        nr     (database updated : 2007.02.20 )
             BLAST carbamoyl-phosphate synthase small subunit [Escherichia coli O157:H7 EDL933]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    GATASE_TYPE_I     

    Other Cross-Reference
        COG COG0505EF 
        EcoCyc carA 

    MMBR References
    J Bacteriol. 1980;143(2):914-20.
    Patterns of polarity in the Escherichia coli car AB gene cluster.
    Gigot D, Crabeel M, Feller A, Charlier D, Lissens W, Glansdorff N, Pierard A. ( 6162837 )
    Proc Natl Acad Sci U S A. 1984;81:4134-4138
    DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoylphosphate.
    Piette, J., H. Nyunoya, C. J. Lusty, R. Cunin, G. Weyens, M. Crabeel, D. Charlier, N. Glansdorff, and A. Pierard.
    Proc Natl Acad Sci U S A. 1984;81:4139-4143
    Multiple regulatory signals in the control region of the Escherichia coli carAB operon.
    Bouvier, J., J. C. Patte, and P. Stragier.
    J Bacteriol. 1980;143(2):921-5.
    Use of gene cloning to determine polarity of an operon: genes carAB of Escherichia coli.
    Crabeel M, Charlier D, Weyens G, Feller A, Pierard A, Glansdorff N. ( 6451616 )

    Sequences
    Amino acid
    FASTA format
    0001 MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR QIVTLTYPHI GNVGTNDADE 
    0071 ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS YLKRHNIVAI ADIDTRKLTR LLREKGAQNG CIIAGDNPDA 
    0141 ALALEKARAF PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG LPEAKKEDEL PFHVVAYDFG AKRNILRMLV 
    0211 DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN GPGDPAPCDY AITAIQKFLE TDIPVFGICL GHQLLALASG 
    0281 AKTVKMKFGH HGGNHPVKDV EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF 
    0351 QGHPEASPGP HDAAPLFDHF IELIEQYRKT AK
    
    
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp
    -100                                             GTTTGCCAGA AATTCGTCGG TAAGCAGATT 
    -070 TGCATTGATT TACGTCATCA TTGTGAATTA ATATGCAAAT AAAGTGAGTG AATATTCTCT GGAGGGTGTT 
    0001 ttgattaagt cagcgctatt ggttctggaa gacggaaccc agtttcacgg tcgggccata ggggcaacag 
    0071 gttcggcggt tggggaagtc gttttcaata cttcaatgac cggttatcaa gaaatcctca ctgatccttc 
    0141 ctattctcgt caaatcgtta ctcttactta tccccatatt ggcaatgtcg gcaccaatga cgccgatgaa 
    0211 gaatcttctc aggtacatgc acaaggtctg gtgattcgcg acctgccgct gattgccagc aacttccgta 
    0281 ataccgaaga cctctcttct tacctgaaac gccataacat cgtggcgatt gccgatatcg atacccgtaa 
    0351 gctgacgcgt ttactgcgcg agaaaggcgc acagaatggc tgcattatcg cgggcgataa cccggatgcg 
    0421 gcgctggcgt tagaaaaagc ccgcgcgttc ccaggtctga atggcatgga tctggcaaaa gaagtgacca 
    0491 ccgcagaagc ctatagctgg acacaaggga gctggacgtt gaccggtggc ctgccagaag cgaaaaaaga 
    0561 agacgagctg ccgttccacg tcgtggctta tgattttggt gccaagcgca acatcctgcg gatgctggtg 
    0631 gatagaggct gtcgcctgac catcgttccg gcgcaaactt ctgcggaaga tgtgctgaaa atgaatccag 
    0701 acggcatctt cctctccaac ggtcctggcg acccggcccc gtgcgattac gccattaccg ccatccagaa 
    0771 attcctcgaa accgatattc cggtattcgg catctgtctc ggtcatcagc tgctggcgct ggcgagcggt 
    0841 gcgaagactg tcaaaatgaa atttggtcac cacggcggca accatccggt taaagatgtg gagaaaaacg 
    0911 tggtaatgat caccgcccag aaccacggtt ttgcggtgga cgaagcaaca ttacctgcaa acctgcgtgt 
    0981 cacgcataaa tccctgttcg acggtacgtt acagggcatt catcgcaccg ataaaccggc attcagcttc 
    1051 caggggcacc ctgaagccag ccctggtcca cacgacgccg cgccgttgtt cgaccacttt atcgagttaa 
    1121 ttgagcagta ccgtaaaacc gctaagtaaT CAGGAGTAAA AGAGCCATGC CAAAACGTAC AGATATAAAA 
    1191 AGTATCCTGA TTCTGGGTGC GGGCCCGATT GTTATCGGTC AGGCGTGTGA GTTTGACTA